公开(公告)号：US5510327A

公开(公告)日：1996-04-23

申请号：US198893

申请日：1994-02-18

申请人： Hitoshi Hayasaka ,  Nobuyuki Kawashima ,  Masatsugu Ueda ,  Eitaro Kumazawa

发明人： Hitoshi Hayasaka ,  Nobuyuki Kawashima ,  Masatsugu Ueda ,  Eitaro Kumazawa

IPC分类号： A61K9/08 ,  A61K38/00 ,  A61K38/18 ,  A61K47/02 ,  A61K47/12 ,  A61K47/18 ,  A61K47/42 ,  A61P1/16 ,  A61P13/12 ,  A61P17/02 ,  A61P35/00 ,  C07K14/52 ,  A61K38/17

CPC分类号： A61K38/1816 ,  A61K38/1833 ,  A61K47/02 ,  A61K47/12 ,  A61K47/183 ,  A61K9/0019 ,  C07K14/52 ,  Y10S514/97

摘要： This invention provides pharmaceutical preparations containing highly concentrated tumor cytotoxic factor (TCF). The pharmaceutical preparations of the present invention contain highly concentrated TCF and a basic amino acid or their salts, or an organic or inorganic salt as a solubizer. The resultant compositions described in the present invention dissolve TCF at high concentrations of 10 mg/ml or over under about neutral and isotonic conditions. The resultant compositions are stable and are suitable for injection preparations.

摘要翻译： 本发明提供含有高度浓缩的肿瘤细胞毒性因子（TCF）的药物制剂。 本发明的药物制剂含有高度浓缩的TCF和碱性氨基酸或其盐，或作为增溶剂的有机或无机盐。 本发明中所述的所得组合物在大约中性和等渗条件下以高浓度10mg / ml或更高浓度溶解TCF。 所得组合物是稳定的并且适合于注射制剂。

公开(公告)号：US4904601A

公开(公告)日：1990-02-27

申请号：US309567

申请日：1989-02-13

申请人： Takashi Mano ,  Akihiko Murakami ,  Masatsugu Ueda ,  Eitaro Kumazawa

发明人： Takashi Mano ,  Akihiko Murakami ,  Masatsugu Ueda ,  Eitaro Kumazawa

IPC分类号： C12M1/06 ,  C12M1/09 ,  C12M3/02

CPC分类号： C12M29/04 ,  C12M25/16 ,  C12M27/02

摘要： Here is disclosed a cell culture tank comprising a cylindrical screen mounted upright within the cell culture tank and having upper and lower openings through which culture medium flows, a pipe for supply of oxygen or oxygen containing gas and an agitator adapted to produce a flow of the culture medium, both the pipe and the agitator being provided within the cylindrical screen, wherein the agitator drives the culture medium to flow downward through the lower opening of the cylindrical screen, then to flow upward into a space defined between the cylindrical screen and the inner wall of the cell culture tank, and a relationship between an ascending velocity of the culture medium and a sinking velocity of the adherent carriers produces in the space between the cylindrical screen and the inner wall of the cell culture tank a floating region within which the adherent carriers float in the culture medium and a separating region within which the adherent carriers are separated from the culture medium.

公开(公告)号：US06399744B1

公开(公告)日：2002-06-04

申请号：US08700519

申请日：1996-08-26

申请人： Masahiko Kinosaki ,  Kyoji Yamaguchi ,  Fumie Kobayashi ,  Masaaki Goto ,  Akihiko Murakami ,  Masatsugu Ueda ,  Yasushi Yamashita ,  Kanji Higashio

发明人： Masahiko Kinosaki ,  Kyoji Yamaguchi ,  Fumie Kobayashi ,  Masaaki Goto ,  Akihiko Murakami ,  Masatsugu Ueda ,  Yasushi Yamashita ,  Kanji Higashio

IPC分类号： C07K1400

CPC分类号： C07K14/4753 ,  A61K38/00

摘要： The present invention relates to a TCF mutant having a novel amino acid sequence which is obtained by mutagenesis of one or more amino acid between N-terminus and the first kringle of the amino acid sequence of native TCF and has lowered affinity to heparin and/or elevated biological activity. The present TCF mutant is prepared by gene manipulation of TCF. The TCF mutants of the present invention have proliferative activity and/or growth stimulative activity in hepatocyte and beneficial as a therapeutic agent for various hepatic diseases and an antitumor agent.

摘要翻译： 本发明涉及具有新氨基酸序列的TCF突变体，其通过在天然TCF的氨基酸序列的N末端与第一个三环体之间的一个或多个氨基酸的诱变获得，并且具有降低的对肝素和/或 升高的生物活性。 本发明的TCF突变体通过TCF的基因操作来制备。 本发明的TCF突变体在肝细胞中具有增殖活性和/或生长刺激活性，并且作为各种肝脏疾病和抗肿瘤剂的治疗剂是有益的。

公开(公告)号：US5648233A

公开(公告)日：1997-07-15

申请号：US290937

申请日：1994-09-29

申请人： Kyoji Yamaguchi ,  Nobuyuki Shima ,  Akihiko Murakami ,  Masaaki Goto ,  Eisuke Tsuda ,  Hiroaki Masunaga ,  Reiko Takahira ,  Fumiko Oogaki ,  Masatsugu Ueda ,  Kanji Higashio

发明人： Kyoji Yamaguchi ,  Nobuyuki Shima ,  Akihiko Murakami ,  Masaaki Goto ,  Eisuke Tsuda ,  Hiroaki Masunaga ,  Reiko Takahira ,  Fumiko Oogaki ,  Masatsugu Ueda ,  Kanji Higashio

IPC分类号： C12N15/09 ,  A61K38/00 ,  A61P35/00 ,  C07K14/475 ,  C07K14/52 ,  C12N1/21 ,  C12N15/12 ,  C12P21/02 ,  C12R1/91 ,  C12P21/06 ,  C07K1/00 ,  C12N1/20

CPC分类号： C07K14/4753 ,  A61K38/00

摘要： Modified TCF in which one or more amino-acid residues of the wild type TCF responsible for glycosylation are substituted or deleted so that at least one N-linked oligosaccharide chain is removed. The modified TCFs have a longer biological half-lives without loss of their biological activities. The modified TCFs are therapeutically important as agents for liver diseases or as anti-cancer drugs.

摘要翻译： PCT No.PCT / JP93 / 01905 Sec。 371日期1994年9月29日 102（e）1994年9月29日PCT 1993年12月27日PCT公布。 第WO94 / 14845号公报 日期1994年7月7日修改TCF，其中负责糖基化的野生型TCF的一个或多个氨基酸残基被取代或缺失，使得至少一个N-连接的寡糖链被除去。 修改过的TCF具有较长的生物半衰期而不丧失生物活性。 修饰的TCF作为肝脏疾病或抗癌药物具有治疗作用。

公开(公告)号：US20060282907A1

公开(公告)日：2006-12-14

申请号：US10543886

申请日：2003-01-30

申请人： Kazuhiro Saeki ,  Kazuya Matsumoto ,  Mikio Kinoshita ,  Iwane Suzuki ,  Yoshitomo Taguchi ,  Koji Mikami ,  Masatsugu Ueda ,  Yoshihiro Hosoi ,  Norio Murata ,  Akira Iritani ,  Kouichiro Kano

发明人： Kazuhiro Saeki ,  Kazuya Matsumoto ,  Mikio Kinoshita ,  Iwane Suzuki ,  Yoshitomo Taguchi ,  Koji Mikami ,  Masatsugu Ueda ,  Yoshihiro Hosoi ,  Norio Murata ,  Akira Iritani ,  Kouichiro Kano

IPC分类号： A01K67/027 ,  C12N15/09 ,  C12N5/06

CPC分类号： C12N15/8509 ,  A01K67/0275 ,  A01K2217/05 ,  A01K2227/105 ,  A01K2227/108 ,  A01K2267/02 ,  A01K2267/0362 ,  A01K2267/0375 ,  C12N9/0032 ,  C12N9/0083

摘要： To provide meat that is beneficial to human health, for the purpose to produce transgenic animals in which the content of unsaturated fatty acids increase is increased, transgenic animals characterized by increased content of unsaturated fatty acids that are beneficial to human health is provided by the present invention. Furthermore, the present invention also provides a method to enhance levels of unsaturated fatty acid in animals.

摘要翻译： 为了提供有益于人类健康的肉类，为了产生其中不饱和脂肪酸含量增加的转基因动物增加的目的，本发明提供了以对人体健康有益的不饱和脂肪酸含量增加为特征的转基因动物 发明。 此外，本发明还提供了增强动物中不饱和脂肪酸水平的方法。

公开(公告)号：US06919434B1

公开(公告)日：2005-07-19

申请号：US09338063

申请日：1999-06-23

申请人： Masaaki Goto ,  Eisuke Tsuda ,  Shin'ichi Mochizuki ,  Kazuki Yano ,  Fumie Kobayashi ,  Nobuyuki Shima ,  Hisataka Yasuda ,  Nobuaki Nakagawa ,  Tomonori Morinaga ,  Masatsugu Ueda ,  Kanji Higashio

发明人： Masaaki Goto ,  Eisuke Tsuda ,  Shin'ichi Mochizuki ,  Kazuki Yano ,  Fumie Kobayashi ,  Nobuyuki Shima ,  Hisataka Yasuda ,  Nobuaki Nakagawa ,  Tomonori Morinaga ,  Masatsugu Ueda ,  Kanji Higashio

IPC分类号： C07K14/705 ,  C12N5/08 ,  C07K16/00 ,  C12Q1/00 ,  G01N33/53

CPC分类号： C07K14/70578 ,  Y10S435/81

摘要： A protein which inhibits osteoclast diffraction and/or maturation and a method for producing the protein. The protein is produced by human embryonic lung fibroblasts and has a molecular weight of about 60 kD and about 120 kD under non-reducing conditions and about 60 kD under reducing conditions an SDS-polyacrylamide gel electrophoresis. The protein can be isolated and purified from the culture medium of fibroblasts. Furthermore, the protein can be produced by gene engineering. The present invention includes cDNA for producing the protein by gene engineering, antibodies having specific affinity for the protein or a method for determining protein concentration using these antibodies.

摘要翻译： 抑制破骨细胞衍生和/或成熟的蛋白质和产生蛋白质的方法。 蛋白质由人类胚胎肺成纤维细胞产生，在非还原条件下分子量约60kD，约120kD，还原条件下分子量约为60kD，SDS-聚丙烯酰胺凝胶电泳。 蛋白质可以从成纤维细胞的培养基中分离和纯化。 此外，蛋白质可以通过基因工程产生。 本发明包括通过基因工程产生蛋白质的cDNA，对蛋白质具有特异性亲和性的抗体或使用这些抗体测定蛋白质浓度的方法。

公开(公告)号：US06855808B2

公开(公告)日：2005-02-15

申请号：US10232858

申请日：2002-09-03

申请人： Masaaki Goto ,  Eisuke Tsuda ,  Shin'ichi Mochizuki ,  Kazuki Yano ,  Fumie Kobayashi ,  Nobuyuki Shima ,  Hisataka Yasuda ,  Nobuaki Nakagawa ,  Tomonori Morinaga ,  Masatsugu Ueda ,  Kanji Higashio

发明人： Masaaki Goto ,  Eisuke Tsuda ,  Shin'ichi Mochizuki ,  Kazuki Yano ,  Fumie Kobayashi ,  Nobuyuki Shima ,  Hisataka Yasuda ,  Nobuaki Nakagawa ,  Tomonori Morinaga ,  Masatsugu Ueda ,  Kanji Higashio

IPC分类号： C07K14/52 ,  A61K38/19 ,  A61P19/10 ,  C07K14/705 ,  C07K16/24 ,  C12N1/21 ,  C12N5/08 ,  C12N15/12 ,  C12N15/19 ,  C12P21/02 ,  C12P21/08 ,  C07K14/51

CPC分类号： C07K14/70578

摘要： A protein which inhibits osteoclast differentiation and/or maturation and a method for producing the protein. The protein is produced by human embryonic lung fibroblasts and has a molecular weight of about 60 kD and about 120 kD under non-reducing conditions and about 60 kD under reducing conditions on SDS-polyacrylamide gel electrophoresis[[, respectively]]. The protein can be isolated and purified from the culture medium of fibroblasts. Furthermore, the protein can be produced by gene engineering. The present invention includes cDNA for producing the protein by gene engineering, antibodies having specific affinity for the protein or a method for determining protein concentration using these antibodies.

摘要翻译： 抑制破骨细胞分化和/或成熟的蛋白质和制备蛋白质的方法。 该蛋白质由人类胚胎肺成纤维细胞产生，在非还原条件下分子量约60kD，约120kD，还原条件下SDS-聚丙烯酰胺凝胶电泳约60kD [分别]。 蛋白质可以从成纤维细胞的培养基中分离和纯化。 此外，蛋白质可以通过基因工程产生。 本发明包括通过基因工程产生蛋白质的cDNA，对蛋白质具有特异性亲和性的抗体或使用这些抗体测定蛋白质浓度的方法。

公开(公告)号：US07468268B2

公开(公告)日：2008-12-23

申请号：US10929748

申请日：2004-08-31

申请人： Masaaki Goto ,  Eisuke Tsuda ,  Shin'ichi Mochizuki ,  Kazuki Yano ,  Fumie Kobayashi ,  Nobuyuki Shima ,  Hisataka Yasuda ,  Nobuaki Nakagawa ,  Tomonori Morinaga ,  Masatsugu Ueda ,  Kanji Higashio

发明人： Masaaki Goto ,  Eisuke Tsuda ,  Shin'ichi Mochizuki ,  Kazuki Yano ,  Fumie Kobayashi ,  Nobuyuki Shima ,  Hisataka Yasuda ,  Nobuaki Nakagawa ,  Tomonori Morinaga ,  Masatsugu Ueda ,  Kanji Higashio

IPC分类号： C12N1/15 ,  C12N1/21 ,  C12N5/10 ,  C12N15/66 ,  C12N15/70 ,  C12N15/74 ,  C12N15/79 ,  C12N15/80 ,  C12N15/85 ,  C12N15/12

CPC分类号： C07K14/70578

摘要： A protein which inhibits osteoclast differentiation and/or maturation and a method for producing the protein. The protein is produced by human embryonic lung fibroblasts and has a molecular weight of about 60 kD and about 120 kD under non-reducing conditions and about 60 kD under reducing conditions on SDS-polyacrylamide gel electrophoresis. The protein can be isolated and purified from the culture medium of fibroblasts. Furthermore, the protein can be produced by gene engineering. The present invention includes cDNA for producing the protein by gene engineering, antibodies having specific affinity for the protein or a method for determining protein concentration using these antibodies.

摘要翻译： 抑制破骨细胞分化和/或成熟的蛋白质和制备蛋白质的方法。 蛋白质由人类胚胎肺成纤维细胞产生，在非还原条件下分子量约为60kD，约120kD，SDS-聚丙烯酰胺凝胶电泳时还原条件下分子量约为60kD。 蛋白质可以从成纤维细胞的培养基中分离和纯化。 此外，蛋白质可以通过基因工程产生。 本发明包括通过基因工程产生蛋白质的cDNA，对蛋白质具有特异性亲和性的抗体或使用这些抗体测定蛋白质浓度的方法。

公开(公告)号：US07205397B2

公开(公告)日：2007-04-17

申请号：US09062113

申请日：1998-04-17

申请人： Masaaki Goto ,  Eisuke Tsuda ,  Shin′ichi Mochizuki ,  Kazuki Yano ,  Fumie Kobayashi ,  Nobuyuki Shima ,  Hisataka Yasuda ,  Nobuaki Nakagawa ,  Tomonori Morinaga ,  Masatsugu Ueda ,  Kanji Higashio

发明人： Masaaki Goto ,  Eisuke Tsuda ,  Shin′ichi Mochizuki ,  Kazuki Yano ,  Fumie Kobayashi ,  Nobuyuki Shima ,  Hisataka Yasuda ,  Nobuaki Nakagawa ,  Tomonori Morinaga ,  Masatsugu Ueda ,  Kanji Higashio

IPC分类号： C07H21/04 ,  C07K14/51

CPC分类号： C07K14/70578

摘要： A protein which inhibits osteoclast differentiation and/or maturation and a method for producing the protein. The protein is produced by human embryonic lung fibroblasts and has a molecular weight of about 60 kD and about 120 kD under non-reducing conditions and about 60 kD under reducing conditions on SDS-polyacrylamide gel electrophoresis. The protein can be isolated and purified from the culture medium of fibroblasts. Furthermore, the protein can be produced by gene engineering. The present invention includes cDNA for producing the protein by gene engineering, antibodies having specific affinity for the protein or a method for determining protein concentration using these antibodies.

摘要翻译： 抑制破骨细胞分化和/或成熟的蛋白质和制备蛋白质的方法。 该蛋白质由人类胚胎肺成纤维细胞产生，在非还原条件下分子量约为60kD，约120kD，SDS-聚丙烯酰胺凝胶电泳时还原条件下分子量约为60kD。 蛋白质可以从成纤维细胞的培养基中分离和纯化。 此外，蛋白质可以通过基因工程产生。 本发明包括通过基因工程产生蛋白质的cDNA，对蛋白质具有特异性亲和性的抗体或使用这些抗体测定蛋白质浓度的方法。

公开(公告)号：US07125686B1

公开(公告)日：2006-10-24

申请号：US08915004

申请日：1997-08-20

申请人： Masaaki Goto ,  Eisuke Tsuda ,  Shin'ichi Mochizuki ,  Kazuki Yano ,  Fumie Kobayashi ,  Nobuyuki Shima ,  Hisataka Yasuda ,  Nobuaki Nakagawa ,  Tomonori Morinaga ,  Masatsugu Ueda ,  Kanji Higashio

发明人： Masaaki Goto ,  Eisuke Tsuda ,  Shin'ichi Mochizuki ,  Kazuki Yano ,  Fumie Kobayashi ,  Nobuyuki Shima ,  Hisataka Yasuda ,  Nobuaki Nakagawa ,  Tomonori Morinaga ,  Masatsugu Ueda ,  Kanji Higashio

IPC分类号： C07K14/00

CPC分类号： C07K14/70578

摘要： A protein which inhibits osteoclast differentiation and/or maturation and a method for producing the protein. The protein is produced by human embryonic lung fibroblasts and has a molecular weight of about 60 kD and about 120 kD under non-reducing conditions and about 60 kD under reducing conditions on SDS-polyacrylamide gel electrophoresis. The protein can be isolated and purified from the culture medium of fibroblasts. Furthermore, the protein can be produced by gene engineering. The present invention includes cDNA for producing the protein by gene engineering, antibodies having specific affinity for the protein or a method for determining protein concentration using these antibodies.

摘要翻译： 抑制破骨细胞分化和/或成熟的蛋白质和制备蛋白质的方法。 蛋白质由人类胚胎肺成纤维细胞产生，在非还原条件下分子量约为60kD，约120kD，SDS-聚丙烯酰胺凝胶电泳时还原条件下分子量约为60kD。 蛋白质可以从成纤维细胞的培养基中分离和纯化。 此外，蛋白质可以通过基因工程产生。 本发明包括通过基因工程产生蛋白质的cDNA，对蛋白质具有特异性亲和性的抗体或使用这些抗体测定蛋白质浓度的方法。