公开(公告)号：US20110196050A1

公开(公告)日：2011-08-11

申请号：US13086741

申请日：2011-04-14

申请人： Hun-joo LEE ,  Ji-na NAMGOONG ,  Soo-suk LEE ,  Joon-ho KIM

发明人： Hun-joo LEE ,  Ji-na NAMGOONG ,  Soo-suk LEE ,  Joon-ho KIM

IPC分类号： B01J41/12 ,  C08G73/02

CPC分类号： C08J5/2218 ,  B01J39/20 ,  B01J41/14 ,  B01J47/016 ,  B01J47/12 ,  B01L3/5027 ,  C08J5/20

摘要： An ion exchangeable mixture containing a polymeric compound consisting of an ion exchange resin, an acrylamide mixture containing at least one bisacrylamide and at least one acrylamide, and a copolymer obtained by reacting the polymeric compound with the acrylamide mixture, and a method of producing the same are provided. The ion exchangeable membrane produced by using the ion exchangeable mixture has significantly smaller electric resistance than conventional ion exchangeable membranes, and has excellent selective permeability because the ion exchangeable membrane is electrically charged. The ion exchangeable membrane can be produced under very mild production conditions, and thus can be produced very easily. Furthermore, the ion exchangeable membrane can be also formed into film during a crosslinking reaction in a solvent of water, and thus is advantageous in that the ion exchangeable membrane can be freely produced into desired sizes, shapes and forms.

摘要翻译： 含有由离子交换树脂，含有至少一种双丙烯酰胺和至少一种丙烯酰胺的丙烯酰胺混合物组成的聚合物的离子交换性混合物和通过使聚合物与丙烯酰胺混合物反应得到的共聚物及其制造方法 被提供。 通过使用离子交换性混合物制造的离子交换膜的电阻比常规的离子交换膜的电阻小得多，并且由于可离子交换膜带电而具有优异的选择性渗透性。 离子交换膜可以在非常温和的生产条件下制备，因此可以非常容易地制备。 此外，在水的溶剂中的交联反应期间，也可以将离子交换膜形成为膜，因此有利的是可离子交换膜可以自由地制成所需的尺寸，形状和形状。

公开(公告)号：US20080073214A1

公开(公告)日：2008-03-27

申请号：US11737448

申请日：2007-04-19

申请人： Hun-joo LEE ,  Soo-suk LEE ,  Joon-ho KIM ,  Sung-ouk JUNG ,  In-ho LEE

发明人： Hun-joo LEE ,  Soo-suk LEE ,  Joon-ho KIM ,  Sung-ouk JUNG ,  In-ho LEE

IPC分类号： B01J47/00 ,  C02F1/42 ,  C02F1/469

CPC分类号： B01D61/422 ,  B01D2311/243 ,  B01D2313/02 ,  B01D2313/10 ,  B01D2313/26 ,  B01D2313/30 ,  B01D2313/50 ,  B01D2321/185

摘要： An electrodialysis apparatus includes a sample chamber including first and second dialysis membranes and filled with a sample between the first dialysis membrane and the second dialysis membrane, an anode chamber including an anode and filled with a first chamber solution between the anode and the first dialysis membrane, and a cathode chamber including a cathode and filled with a second chamber solution between the cathode and the second dialysis membrane. In particular, when a voltage is applied to the anode and the cathode, ionic materials of the sample move to the anode and cathode chambers.

摘要翻译： 电渗析装置包括：样品室，包括第一和第二透析膜，并在第一透析膜和第二透析膜之间填充样品;阳极室，包括阳极，并在阳极和第一透析膜之间填充第一室溶液 以及包括阴极并在阴极和第二透析膜之间填充有第二室溶液的阴极室。 特别地，当向阳极和阴极施加电压时，样品的离子材料移动到阳极室和阴极室。

公开(公告)号：US20100329931A1

公开(公告)日：2010-12-30

申请号：US12833095

申请日：2010-07-09

申请人： Kyu-tae YOO ,  Joon-ho KIM ,  Jun-hong MIN ,  Sung-ouk JUNG ,  Ji-na NAMGOONG ,  Kui-hyun KIM ,  Jeo-young SHIM ,  Kak NAMKOONG

发明人： Kyu-tae YOO ,  Joon-ho KIM ,  Jun-hong MIN ,  Sung-ouk JUNG ,  Ji-na NAMGOONG ,  Kui-hyun KIM ,  Jeo-young SHIM ,  Kak NAMKOONG

IPC分类号： G01N27/00

CPC分类号： G01N33/54373

摘要： Provided are a sensing switch and a sensing method using the same. The sensing switch includes: a substrate; a supporter on the substrate; a sensing plate that is connected to a side of the supporter and is in parallel with the substrate by a predetermined distance; a receptor binding region on an upper surface of an end portion of the sensing plate; an electric or magnetic field generation device that induces deflection of the sensing plate when a receptor bound to the receptor binding region is selectively bound to an electrically or magnetically active ligand; and a pair of switching electrodes that are separated by a predetermined distance and is connected when the sensing plate contacts the substrate due to the deflection of the sensing plate. A target material need not be labelled, a signal processing of a fluorescent or electrical detection signal using an analysis apparatus is not required, and a signal can be directly decoded by confirming whether a current flows through the switch.

摘要翻译： 提供了一种感测开关和使用该感测开关的感测方法。 感测开关包括：基板; 底物上的支撑物; 感测板，其与所述支撑体的一侧连接并且与所述基板平行预定距离; 在感测板的端部的上表面上的受体结合区域; 当与受体结合区结合的受体选择性地结合到电或磁性活性配体时，引起感测板偏转的电场或磁场产生装置; 以及一对分离预定距离的开关电极，并且当感测板由于感测板的偏转而与基板接触时被连接。 目标材料不需要标注，不需要使用分析装置的荧光或电检测信号的信号处理，并且可以通过确认电流是否流过开关直接解码信号。

公开(公告)号：US20090170211A1

公开(公告)日：2009-07-02

申请号：US12272183

申请日：2008-11-17

申请人： Kyu-tae YOO ,  Joon-ho KIM ,  Jun-hong MIN ,  Sung-ouk JUNG ,  Ji-na NAMGOONG ,  Kui-hyun KIM ,  Jeo-young SHIM ,  Kak NAMKOONG

发明人： Kyu-tae YOO ,  Joon-ho KIM ,  Jun-hong MIN ,  Sung-ouk JUNG ,  Ji-na NAMGOONG ,  Kui-hyun KIM ,  Jeo-young SHIM ,  Kak NAMKOONG

IPC分类号： G01N27/00

CPC分类号： G01N33/54373

摘要： Provided are a sensing switch and a sensing method using the same. The sensing switch includes: a substrate; a supporter on the substrate; a sensing plate that is connected to a side of the supporter and is in parallel with the substrate by a predetermined distance; a receptor binding region on an upper surface of an end portion of the sensing plate; an electric or magnetic field generation device that induces deflection of the sensing plate when a receptor bound to the receptor binding region is selectively bound to an electrically or magnetically active ligand; and a pair of switching electrodes that are separated by a predetermined distance and is connected when the sensing plate contacts the substrate due to the deflection of the sensing plate. A target material need not be labelled, a signal processing of a fluorescent or electrical detection signal using an analysis apparatus is not required, and a signal can be directly decoded by confirming whether a current flows through the switch.

摘要翻译： 提供了一种感测开关和使用该感测开关的感测方法。 感测开关包括：基板; 底物上的支撑物; 感测板，其与所述支撑体的一侧连接并且与所述基板平行预定距离; 在感测板的端部的上表面上的受体结合区域; 当与受体结合区结合的受体选择性地结合到电或磁性活性配体时，引起感测板偏转的电场或磁场产生装置; 以及一对分离预定距离的开关电极，并且当感测板由于感测板的偏转而与基板接触时被连接。 目标材料不需要标注，不需要使用分析装置的荧光或电检测信号的信号处理，并且可以通过确认电流是否流过开关直接解码信号。

公开(公告)号：US20120103832A1

公开(公告)日：2012-05-03

申请号：US13348168

申请日：2012-01-11

申请人： Hun-joo LEE ,  Joon-ho KIM ,  Chin-sung PARK ,  Jung-im HAN ,  Seung-yeon YANG ,  Shin-i YOO

发明人： Hun-joo LEE ,  Joon-ho KIM ,  Chin-sung PARK ,  Jung-im HAN ,  Seung-yeon YANG ,  Shin-i YOO

IPC分类号： C25B15/02 ,  B81B1/00

CPC分类号： B01L3/5027 ,  B01L2200/06

摘要： A microfluidic device for electrochemically regulating the pH of a fluid includes: an ion-exchange material; an anode chamber having a surface defined by a surface of the ion-exchange material and an anode electrode disposed along an edge of the surface of the anode chamber; and a cathode chamber having a surface defined by an opposite surface of the ion-exchange material and a cathode electrode disposed along an edge of the surface of the cathode chamber.

摘要翻译： 用于电化学调节流体pH的微流体装置包括：离子交换材料; 具有由所述离子交换材料的表面限定的表面的阳极室和沿着所述阳极室的表面的边缘设置的阳极电极; 以及阴极室，其具有由离子交换材料的相对表面限定的表面和沿着阴极室的表面的边缘设置的阴极电极。

公开(公告)号：US20080044880A1

公开(公告)日：2008-02-21

申请号：US11841191

申请日：2007-08-20

申请人： Jung-im HAN ,  Joon-ho KIM ,  Sung-young JEONG ,  Young-sun LEE ,  Kyu-youn HWANG ,  Hun-joo LEE

发明人： Jung-im HAN ,  Joon-ho KIM ,  Sung-young JEONG ,  Young-sun LEE ,  Kyu-youn HWANG ,  Hun-joo LEE

IPC分类号： C12N13/00 ,  C12M1/00

CPC分类号： C12N1/02 ,  B01D61/48

摘要： A method of separating microorganisms from a sample includes introducing a sample into an apparatus which controls a concentration of at least one salt, the apparatus includes a reaction chamber defined between a cation exchange membrane and an anion exchange membrane, a first electrode chamber defined between the anion exchange membrane and a first electrode, the first electrode chamber containing a first ion exchange medium, a second electrode chamber defined between the cation exchange membrane and a second electrode, the second electrode chamber containing a second ion exchange medium, applying a voltage between the first electrode and the second electrode to electrodialyze the sample in the reaction chamber and reduce the concentration of the at least one salt in the sample and allowing the sample having the reduced concentration of the at least one salt to contact a microorganism capturing means.

摘要翻译： 从样品中分离微生物的方法包括将样品引入到控制至少一种盐浓度的装置中，该装置包括界定在阳离子交换膜和阴离子交换膜之间的反应室，第一电极室 阴离子交换膜和第一电极，第一电极室包含第一离子交换介质，第二电极室限定在阳离子交换膜和第二电极之间，第二电极室包含第二离子交换介质， 第一电极和第二电极，使反应室中的样品电渗析，并降低样品中至少一种盐的浓度，并允许具有降低的至少一种盐浓度的样品接触微生物捕获装置。

公开(公告)号：US20080044864A1

公开(公告)日：2008-02-21

申请号：US11841107

申请日：2007-08-20

申请人： Sung-young JEONG ,  Kyu-youn HWANG ,  Joon-ho KIM ,  Jung-im HAN ,  Hun-joo LEE

发明人： Sung-young JEONG ,  Kyu-youn HWANG ,  Joon-ho KIM ,  Jung-im HAN ,  Hun-joo LEE

IPC分类号： C12P19/34

CPC分类号： C12N15/1006 ,  B01L7/52 ,  B01L2300/0636 ,  C12N2533/30 ,  C12Q1/6806

摘要： Provided is a method of amplifying nucleic acid from a comprising: contacting a cell-containing sample with a nonplanar solid substrate in a liquid medium having a pH range of 3.0-6.0 to attach the cell to the solid substrate; washing the nonplanar solid substrate to remove materials that are not attached thereto; and performing PCR using the nucleic acid from the cell attached to the nonplanar solid substrate as a template sample to amplify nucleic acid from the cell, wherein the contacting, washing and PCR processes are performed in a single vessel.

摘要翻译： 本发明提供一种扩增来自a的核酸的方法，其特征在于：在pH范围为3.0-6.0的液体介质中使含细胞的样品与非平面固体基质接触，以将该细胞附着在固体基质上; 洗涤非平面固体基质以除去未附着于其上的材料; 以及使用来自附着在所述非平面固体底物上的细胞的核酸作为模板样品进行PCR以扩增来自所述细胞的核酸，其中所述接触，洗涤和PCR过程在单个容器中进行。

公开(公告)号：US20070237680A1

公开(公告)日：2007-10-11

申请号：US11670002

申请日：2007-02-01

申请人： Hun-joo LEE ,  Joon-ho KIM ,  Sung-young JEONG ,  Kyu-youn HWANG

发明人： Hun-joo LEE ,  Joon-ho KIM ,  Sung-young JEONG ,  Kyu-youn HWANG

IPC分类号： G01N33/00

CPC分类号： C12M47/02 ,  C12M47/06

摘要： A microfluidic device for concentrating a sample including cells or viruses and lysing the cells or viruses, the device including an anode chamber including an anode electrode, a cathode chamber including a cathode electrode and an ion exchange membrane separating the anode chamber and the cathode chamber. The cathode chamber includes a solid support therein. A method of producing the microfluidic device and a method of concentrating a sample including cells or viruses and lysing the cells or viruses therein using the microfluidic device.

摘要翻译： 一种用于浓缩包含细胞或病毒的样品并裂解细胞或病毒的微流体装置，所述装置包括包括阳极电极的阳极室，包括阴极的阴极室和分离阳极室和阴极室的离子交换膜。 阴极室包括固体支撑体。 微流体装置的制造方法以及浓缩包含细胞或病毒的样品并使用微流体装置使细胞或病毒裂解的方法。

公开(公告)号：US20120255871A1

公开(公告)日：2012-10-11

申请号：US13495342

申请日：2012-06-13

申请人： Hun-joo LEE ,  Jin-tae KIM ,  Sung-young JEONG ,  Joon-ho KIM ,  Hee-kyun LIM

发明人： Hun-joo LEE ,  Jin-tae KIM ,  Sung-young JEONG ,  Joon-ho KIM ,  Hee-kyun LIM

IPC分类号： C25B15/00

CPC分类号： G05D21/02

摘要： A pH adjusting apparatus includes an electrolytic chip receiving a solution, an electrolytic chip loading station receiving the electrolytic chip, an input unit inputting electrolysis conditions, a control unit receiving the electrolysis conditions and controlling electrolysis performed in the electrolytic chip, and a display unit displaying the electrolysis conditions and a progress of the electrolysis. Thus, the pH of a solution can be adjusted easily and accurately, by precisely controlling a constant current, a constant voltage, and current and voltage application times, thereby enabling useful application in various biological assays such as cell lysis. Furthermore, the pH adjusting apparatus has small size and weight and can be operated for a long time after charging once due to low power consumption.

摘要翻译： pH调节装置包括：接收溶液的电解芯片，接收电解芯片的电解芯片装载站，输入电解条件的输入单元，接收电解条件的控制单元以及控制电解芯片中进行的电解;以及显示单元， 电解条件和电解进度。 因此，通过精确地控制恒定电流，恒定电压，电流和电压施加时间，可以容易且精确地调节溶液的pH，从而可用于各种生物测定如细胞裂解中的有用应用。 此外，pH调节装置的体积小，重量轻，能够在低功耗的情况下一次充电后长时间运转。

公开(公告)号：US20070151864A1

公开(公告)日：2007-07-05

申请号：US11559924

申请日：2006-11-15

申请人： Hun-joo LEE ,  Jin-tae KIM ,  Sung-young JEONG ,  Joon-ho KIM ,  Hee-kyun LIM

发明人： Hun-joo LEE ,  Jin-tae KIM ,  Sung-young JEONG ,  Joon-ho KIM ,  Hee-kyun LIM

IPC分类号： C25B1/00

CPC分类号： G05D21/02

摘要： A pH adjusting apparatus includes an electrolytic chip receiving a solution, an electrolytic chip loading station receiving the electrolytic chip, an input unit inputting electrolysis conditions, a control unit receiving the electrolysis conditions and controlling electrolysis performed in the electrolytic chip, and a display unit displaying the electrolysis conditions and a progress of the electrolysis. Thus, the pH of a solution can be adjusted easily and accurately, by precisely controlling a constant current, a constant voltage, and current and voltage application times, thereby enabling useful application in various biological assays such as cell lysis. Furthermore, the pH adjusting apparatus has small size and weight and can be operated for a long time after charging once due to low power consumption.

摘要翻译： pH调节装置包括：接收溶液的电解芯片，接收电解芯片的电解芯片装载站，输入电解条件的输入单元，接收电解条件的控制单元以及控制电解芯片中进行的电解;以及显示单元， 电解条件和电解进度。 因此，通过精确地控制恒定电流，恒定电压，电流和电压施加时间，可以容易且精确地调节溶液的pH，从而可用于各种生物测定如细胞裂解中的有用应用。 此外，pH调节装置的体积小，重量轻，能够在低功耗的情况下一次充电后长时间运转。