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    Kinetic exclusion amplification of nucleic acid libraries
    2.
    发明授权
    Kinetic exclusion amplification of nucleic acid libraries 有权

    公开(公告)号：US10808277B2

    公开(公告)日：2020-10-20

    申请号：US15844051

    申请日：2017-12-15

    Applicant: Illumina, Inc. ,  Illumina Cambridge Limited

    Inventor： Shaun Hunter ,  Peter McInerney ,  Jonathan Boutell ,  Claire Bevis-Mott

    IPC: C12Q1/68 ,  B01J19/00 ,  B01L3/00 ,  C12Q1/6848 ,  C12Q1/6874 ,  C12Q1/6806

    Abstract: An example method includes reacting a first solution and a different, second solution on a flow cell by flowing the first solution over amplification sites on the flow cell and subsequently flowing the second solution over the amplification sites. The first solution includes target nucleic acids and a first reagent mixture that comprises nucleoside triphosphates and replication enzymes. The target nucleic acids in the first solution transport to and bind to the amplification sites at a transport rate. The first reagent mixture amplifies the target nucleic acids that are bound to the amplification sites to produce clonal populations of amplicons originating from corresponding target nucleic acids. The amplicons are produced at an amplification rate that exceeds the transport rate. The second solution includes a second reagent mixture and lacks the target nucleic acids. The second solution is to increase a number of the amplicons at the amplification sites.

    ON-SEQUENCER FLOWCELL REUSE
    3.
    发明公开
    ON-SEQUENCER FLOWCELL REUSE 审中-公开

    公开(公告)号：US20240309423A1

    公开(公告)日：2024-09-19

    申请号：US18595944

    申请日：2024-03-05

    Applicant: Illumina, Inc.

    Inventor： Jonathan Boutell ,  Katharina Mueller-Ott ,  Jason Betley ,  Xiaolin Wu ,  Wayne George ,  Pietro Gatti Lafranconi ,  Andrew Brown

    IPC: C12Q1/44 ,  C12Q1/6806

    CPC classification number: C12Q1/44 ,  C12Q1/6806

    Abstract: Automated methods conducted in a sequencing flowcell, and kits for reusing a flowcell, are provided herein. In some examples, an automated method conducted in a sequencing flowcell may include, at a surface of the sequencing flowcell coupled to a first moiety, using a reagent to decouple a first complex from the first moiety. In some examples, the first complex may include a second moiety which couples to the first moiety and a polynucleotide coupled to the second moiety. In some examples, the method may further include using a nuclease to polynucleotides in the sequencing flowcell. The method may include, after using the reagent and after using the nuclease, coupling a second complex to the first moiety. The second complex may include a third moiety which couples with the first moiety and an oligonucleotide coupled to the third moiety.

    METHODS FOR IMPROVING NUCLEIC ACID CLUSTER CLONALITY
    6.
    发明申请
    METHODS FOR IMPROVING NUCLEIC ACID CLUSTER CLONALITY 有权

    公开(公告)号：US20220333178A1

    公开(公告)日：2022-10-20

    申请号：US17699573

    申请日：2022-03-21

    Applicant: Illumina Cambridge Limited ,  Illumina, Inc.

    Inventor： Gary Mark Skinner ,  Geraint Evans ,  Niall Gormley ,  Jonathan Boutell ,  Matthew W. Kellinger ,  Michael Previte ,  Molly He

    IPC: C12Q1/6848 ,  C12Q1/70

    Abstract: A method for seeding and amplifying target nucleic acids derived from a sample in a cluster at a site on a surface of a substrate includes retaining at least a portion of the target nucleic acids in an inactive form that cannot seed to provide a relatively low concentration of active form target nucleic acids available for seeding. As the active form target nucleic acids seed on the surface of the substrate, they may be amplified. Because the concentration of active form target nucleic acids is low, the likelihood is low that a second active form target nucleic acid will seed at the same site within the same cluster before the first active form target nucleic acid is sufficiently amplified to dominate. Accordingly, the likelihood that the cluster will pass filters is increased relative to traditional seeding and amplification methods employing a higher concentration of active form target nucleic acids.

    KINETIC EXCLUSION AMPLIFICATION OF NUCLEIC ACID LIBRARIES
    7.
    发明申请
    KINETIC EXCLUSION AMPLIFICATION OF NUCLEIC ACID LIBRARIES 有权

    公开(公告)号：US20210010071A1

    公开(公告)日：2021-01-14

    申请号：US17033133

    申请日：2020-09-25

    Applicant: Illumina, Inc. ,  Illumina Cambridge Limited

    Inventor： Shaun Hunter ,  Peter McInerney ,  Jonathan Boutell ,  Claire Bevis-Mott

    IPC: C12Q1/6848 ,  B01J19/00 ,  C12Q1/6874 ,  B01L3/00 ,  C12Q1/6806

    Abstract: An example method includes reacting a first solution and a different, second solution on a flow cell by flowing the first solution over amplification sites on the flow cell and subsequently flowing the second solution over the amplification sites. The first solution includes target nucleic acids and a first reagent mixture that comprises nucleoside triphosphates and replication enzymes. The target nucleic acids in the first solution transport to and bind to the amplification sites at a transport rate. The first reagent mixture amplifies the target nucleic acids that are bound to the amplification sites to produce clonal populations of amplicons originating from corresponding target nucleic acids. The amplicons are produced at an amplification rate that exceeds the transport rate. The second solution includes a second reagent mixture and lacks the target nucleic acids. The second solution is to increase a number of the amplicons at the amplification sites.

    ON-SEQUENCER IMAGING FLOWCELL REUSE
    8.
    发明申请
    ON-SEQUENCER IMAGING FLOWCELL REUSE 有权

    公开(公告)号：US20250115957A1

    公开(公告)日：2025-04-10

    申请号：US18897718

    申请日：2024-09-26

    Applicant: Illumina, Inc.

    Inventor： Claudia Battistella ,  Gabriele Canzi ,  Katharina Mueller-Ott ,  Wayne George ,  Jonathan Boutell

    IPC: C12Q1/6874

    Abstract: Some examples herein provide a sequencing flowcell that includes an imaging sensor; a hydrogel disposed on the imaging sensor and comprising a moiety; and a first complex non-covalently coupled to the moiety, the first complex comprising a first oligonucleotide. A method of using the sequencing flowcell may include decoupling the first complex from the moiety; and coupling a second complex to the moiety, the second complex comprising a second oligonucleotide. Methods of forming the flowcell are also provided.

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