公开(公告)号：US20160199832A1

公开(公告)日：2016-07-14

申请号：US14912394

申请日：2014-08-29

申请人： ILLUMINA, INC. ,  ADVANCED LIQUID LOGIC FRANCE SAS

发明人： Arash Jamshidi ,  Yan-You Lin ,  Alex Aravanis ,  Cyril Delattre ,  Arnaud Rival ,  Jennifer Foley ,  Poorya Sabounchi ,  Tarun Khurana ,  Majid Babazadeh ,  Hamed Amini ,  Bala Murali Venkatesan ,  M. Shane Bowen ,  Steven M. Barnard ,  Maria Candelaria Rogert Bacigalupo ,  Dietrich Dehlinger

IPC分类号： B01L3/00

CPC分类号： B01L3/50273 ,  B01F13/0071 ,  B01F13/0076 ,  B01F13/0084 ,  B01L3/502792 ,  B01L2200/06 ,  B01L2300/0636 ,  B01L2300/0819 ,  B01L2300/0858 ,  B01L2300/087 ,  B01L2300/161 ,  B01L2300/166 ,  B01L2400/0427 ,  B41J2/04 ,  G01N15/1463 ,  G01N15/1475 ,  G01N2015/1006

摘要： Provided herein is a droplet actuator including (a) first and second substrates separated by a droplet-operations gap, the first and second substrates including respective hydrophobic surfaces that face the droplet-operations gap; (b) a plurality of electrodes coupled to at least one of the first substrate and the second substrate, the electrodes arranged along the droplet-operations gap to control movement of a droplet along the hydrophobic surfaces within the droplet-operations gap; and (c) a hydrophilic or variegated-hydrophilic surface exposed to the droplet-operations gap, the hydrophilic or variegated-hydrophilic surface being positioned to contact the droplet when the droplet is at a select position within the droplet-operations gap.

摘要翻译： 本文提供的液滴致动器包括（a）由液滴操作间隙分离的第一和第二基板，第一和第二基板包括面对液滴操作间隙的各个疏水表面; （b）耦合到第一衬底和第二衬底中的至少一个的多个电极，电极沿着液滴操作间隙排列，以控制液滴沿着液滴操作间隙内的疏水表面的运动; 和（c）暴露于液滴操作间隙的亲水或杂色亲水表面，当液滴在液滴操作间隙内的选择位置时，亲水或杂色亲水表面定位成接触液滴。

公开(公告)号：US11123733B2

公开(公告)日：2021-09-21

申请号：US16691405

申请日：2019-11-21

申请人： Illumina, Inc.

发明人： Hamed Amini ,  Arash Jamshidi ,  Tarun Kumar Khurana ,  Foad Mashayekhi ,  Yir-Shyuan Wu

IPC分类号： B01L3/00 ,  B01F3/08 ,  B01F13/00

摘要： Described are microfluidic devices and methods for providing a predetermined number of microspheres or beads, together with a cell, within a fluid droplet being processed. The system may provide each droplet with a single bead and a single cell, and the bead may contain DNA or other reagents for later identifying the specific cell associated with that bead.

公开(公告)号：US20200086322A1

公开(公告)日：2020-03-19

申请号：US16691405

申请日：2019-11-21

申请人： Illumina, Inc.

发明人： Hamed Amini ,  Arash Jamshidi ,  Tarun Kumar Khurana ,  Foad Mashayekhi ,  Yir-Shyuan Wu

IPC分类号： B01L3/00 ,  B01F13/00 ,  B01F3/08

摘要： Described are microfluidic devices and methods for providing a predetermined number of microspheres or beads, together with a cell, within a fluid droplet being processed. The system may provide each droplet with a single bead and a single cell, and the bead may contain DNA or other reagents for later identifying the specific cell associated with that bead.

公开(公告)号：US20170128940A1

公开(公告)日：2017-05-11

申请号：US15347709

申请日：2016-11-09

申请人： Illumina, Inc.

发明人： Hamed Amini ,  Arash Jamshidi ,  Tarun Kumar Khurana ,  Foad Mashayekhi ,  Yir-Shyuan Wu

IPC分类号： B01L3/00

CPC分类号： B01L3/502761 ,  B01F3/0807 ,  B01F13/0062 ,  B01J2219/00468 ,  B01J2219/005 ,  B01J2219/00722 ,  B01L3/502776 ,  B01L3/502784 ,  B01L2200/0636 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2300/0816 ,  B01L2300/0858 ,  B01L2300/0867 ,  B01L2300/0883

摘要： Described are microfluidic devices and methods for providing a predetermined number of microspheres or beads, together with a cell, within a fluid droplet being processed. The system may provide each droplet with a single bead and a single cell, and the bead may contain DNA or other reagents for later identifying the specific cell associated with that bead.