公开(公告)号：US11543340B2

公开(公告)日：2023-01-03

申请号：US16574693

申请日：2019-09-18

Applicant: Iris International, Inc.

Inventor： Bart J. Wanders ,  Brett Jordan ,  Gregory A. Farrell ,  Thomas H. Adams ,  Warren Groner

IPC: G01N15/14 ,  G01N33/50 ,  G01N15/06 ,  G01N21/53 ,  G02B7/36 ,  G01N33/80 ,  G01N33/49 ,  G01N15/00 ,  G01N15/10 ,  G01N21/05 ,  G02B21/24 ,  G06T7/00 ,  G02B7/28 ,  G01N1/30

Abstract: Particles such as blood cells can be categorized and counted by a digital image processor. A digital microscope camera can be directed into a flowcell defining a symmetrically narrowing flowpath in which the sample stream flows in a ribbon flattened by flow and viscosity parameters between layers of sheath fluid. A contrast pattern for autofocusing is provided on the flowcell, for example at an edge of a rear illumination opening. The image processor assesses focus accuracy from pixel data contrast. A positioning motor moves the microscope and/or flowcell along the optical axis for autofocusing on the contrast pattern target. The processor then displaces microscope and flowcell by a known distance between the contrast pattern and the sample stream, thus focusing on the sample stream. Blood cell images are collected from that position until autofocus is reinitiated, periodically, by input signal, or when detecting temperature changes or focus inaccuracy in the image data.

公开(公告)号：US20140273068A1

公开(公告)日：2014-09-18

申请号：US14216811

申请日：2014-03-17

Applicant: IRIS International, Inc.

Inventor： Bart J. Wanders ,  Brett Jordan ,  Gregory A. Farrell ,  Thomas H. Adams ,  Warren Groner

IPC: G01N33/50

Abstract: Particles such as blood cells can be categorized and counted by a digital image processor. A digital microscope camera can be directed into a flowcell defining a symmetrically narrowing flowpath in which the sample stream flows in a ribbon flattened by flow and viscosity parameters between layers of sheath fluid. A contrast pattern for autofocusing is provided on the flowcell, for example at an edge of a rear illumination opening. The image processor assesses focus accuracy from pixel data contrast. A positioning motor moves the microscope and/or flowcell along the optical axis for autofocusing on the contrast pattern target. The processor then displaces microscope and flowcell by a known distance between the contrast pattern and the sample stream, thus focusing on the sample stream. Blood cell images are collected from that position until autofocus is reinitiated, periodically, by input signal, or when detecting temperature changes or focus inaccuracy in the image data.

Abstract translation: 可以通过数字图像处理器对诸如血细胞的颗粒进行分类和计数。 数字显微镜照相机可以被引导到限定对称变窄的流动路径的流动池中，其中样品流在由鞘液层之间的流动和粘度参数平坦化的带中流动。 在流动池上提供用于自动聚焦的对比度图案，例如在后部照明开口的边缘。 图像处理器从像素数据对比度评估焦点精度。 定位电机沿着光轴移动显微镜和/或流动池，用于自动聚焦在对比度图案目标上。 然后，处理器将显微镜和流动池移位在对比度图案和样品流之间已知的距离，从而聚焦于样品流。 从该位置收集血细胞图像，直到通过输入信号周期性重新开始自动聚焦，或当检测图像数据中的温度变化或聚焦不准确时。

公开(公告)号：US20200080926A1

公开(公告)日：2020-03-12

申请号：US16574693

申请日：2019-09-18

Applicant: Iris International, Inc.

Inventor： Bart J. Wanders ,  Brett Jordan ,  Gregory A. Farrell ,  Thomas H. Adams ,  Warren Groner

IPC: G01N15/14 ,  G01N33/80 ,  G01N33/50 ,  G01N33/49 ,  G01N21/53 ,  G01N15/06 ,  G01N1/30

Abstract: Particles such as blood cells can be categorized and counted by a digital image processor. A digital microscope camera can be directed into a flowcell defining a symmetrically narrowing flowpath in which the sample stream flows in a ribbon flattened by flow and viscosity parameters between layers of sheath fluid. A contrast pattern for autofocusing is provided on the flowcell, for example at an edge of a rear illumination opening. The image processor assesses focus accuracy from pixel data contrast. A positioning motor moves the microscope and/or flowcell along the optical axis for autofocusing on the contrast pattern target. The processor then displaces microscope and flowcell by a known distance between the contrast pattern and the sample stream, thus focusing on the sample stream. Blood cell images are collected from that position until autofocus is reinitiated, periodically, by input signal, or when detecting temperature changes or focus inaccuracy in the image data.

公开(公告)号：US10060846B2

公开(公告)日：2018-08-28

申请号：US15645710

申请日：2017-07-10

Applicant: Iris International, Inc.

Inventor： Bart J. Wanders ,  Gregory A. Farrell ,  Thomas H. Adams ,  Warren Groner ,  Xiaodong Zhao ,  Brett Jordan ,  Jack Cremins ,  Carol Quon

IPC: G01N15/14 ,  G01N33/50 ,  G01N33/49 ,  G01N21/53 ,  G01N1/30 ,  G01N33/80 ,  G01N21/05 ,  G01N15/10 ,  G01N15/00

CPC classification number: G01N15/1404 ,  G01N1/30 ,  G01N15/06 ,  G01N15/1434 ,  G01N15/1463 ,  G01N15/1468 ,  G01N15/147 ,  G01N15/1475 ,  G01N21/53 ,  G01N33/49 ,  G01N33/4915 ,  G01N33/5091 ,  G01N33/5094 ,  G01N33/80 ,  G01N2015/0065 ,  G01N2015/1037 ,  G01N2015/1411 ,  G01N2015/1413 ,  G01N2015/1452 ,  G01N2015/1486 ,  G01N2021/058

Abstract: Aspects and embodiments of the instant disclosure provide a particle and/or intracellular organelle alignment agent for a particle analyzer used to analyze particles contained in a sample. An exemplary particle and/or intracellular organelle alignment agent includes an aqueous solution, a viscosity modifier, and/or a buffer. Embodiments also encompass systems, compositions, and methods for analyzing a sample containing particles. Parrticles such as blood cells can be categorized and counted by a digital image processor. A digital microscope camera can be directed, for example using certain focusing techniques, into a flowcell defining a symmetrically narrowing flowpath in which the sample stream flows in a ribbon flattened by flow and viscosity parameters between layers of sheath fluid. Blood cell images can be collected and analyzed using dynamic range extension processes and systems.

公开(公告)号：US20160187246A1

公开(公告)日：2016-06-30

申请号：US15064129

申请日：2016-03-08

Applicant: IRIS International, Inc.

Inventor： Gregory A. Farrell ,  Bart J. Wanders ,  Thomas H. Adams ,  Warren Groner ,  Xiaodong Zhao

IPC: G01N15/14 ,  G01N33/50

CPC classification number: G01N33/4915 ,  G01N15/1404 ,  G01N15/1434 ,  G01N15/1436 ,  G01N15/1459 ,  G01N15/1463 ,  G01N15/1468 ,  G01N15/147 ,  G01N15/1475 ,  G01N21/05 ,  G01N33/5091 ,  G01N2015/0065 ,  G01N2015/0073 ,  G01N2015/008 ,  G01N2015/0084 ,  G01N2015/1006 ,  G01N2015/1037 ,  G01N2015/1409 ,  G01N2015/1411 ,  G01N2015/1413 ,  G01N2015/1452 ,  G01N2015/1486 ,  G01N2021/058 ,  G01N2201/12

Abstract: Aspects and embodiments of the instant disclosure provide a particle and/or intracellular organelle alignment agent for a particle analyzer used to analyze particles contained in a sample. An exemplary particle and/or intracellular organelle alignment agent includes an aqueous solution, a viscosity modifier, and/or a buffer.

Abstract translation: 本公开的方面和实施方案提供用于分析样品中包含的颗粒的颗粒分析仪的颗粒和/或细胞内细胞器取向剂。 示例性的颗粒和/或细胞内细胞器取向剂包括水溶液，粘度调节剂和/或缓冲液。

公开(公告)号：US20140273067A1

公开(公告)日：2014-09-18

申请号：US14216533

申请日：2014-03-17

Applicant: IRIS International, Inc.

Inventor： Bart J. Wanders ,  Thomas H. Adams ,  Gregory A. Farrell ,  Warren Groner ,  Xiaodong Zhao

IPC: G01N33/50

CPC classification number: G01N15/1404 ,  G01N1/30 ,  G01N15/1434 ,  G01N15/1463 ,  G01N15/1468 ,  G01N15/147 ,  G01N15/1475 ,  G01N21/53 ,  G01N33/49 ,  G01N33/4915 ,  G01N33/5091 ,  G01N33/5094 ,  G01N33/80 ,  G01N2015/0065 ,  G01N2015/1037 ,  G01N2015/1411 ,  G01N2015/1413 ,  G01N2015/1452 ,  G01N2015/1486 ,  G01N2021/058

Abstract: The present disclosure relates to apparatus, systems, compositions, and methods for analyzing a sample containing particles. In some aspects the system comprises an analyzer which may be a visual analyzer. In one aspect, this disclosure relates to a particle imaging system comprising a flowcell through which a sample containing particles is caused to flow, and a high optical resolution imaging device which captures images for image analysis of samples. Other compositions, methods and features of this disclosure are disclosed herein.

Abstract translation: 本公开涉及用于分析含有颗粒的样品的装置，系统，组合物和方法。 在一些方面，该系统包括可以是视觉分析仪的分析器。 一方面，本发明涉及一种粒子成像系统，该粒子成像系统包括流过细胞，通过该流通池使含有颗粒的样品流动;以及高光学分辨率成像装置，其捕获用于样品的图像分析的图像。 本公开的其它组合物，方法和特征在此公开。

公开(公告)号：US10451612B2

公开(公告)日：2019-10-22

申请号：US15263001

申请日：2016-09-12

Applicant: IRIS International, Inc.

Inventor： Gregory A. Farrell ,  Bart J. Wanders ,  Thomas H. Adams ,  Warren Groner ,  Xiaodong Zhao

IPC: G01N33/49 ,  G01N33/50 ,  G01N15/14 ,  G01N21/05 ,  G01N15/00 ,  G01N15/10

Abstract: Aspects and embodiments of the instant disclosure provide a particle and/or intracellular organelle alignment agent for a particle analyzer used to analyze particles contained in a sample. An exemplary particle and/or intracellular organelle alignment agent includes an aqueous solution, a viscosity modifier, and/or a buffer.

公开(公告)号：US20170370820A1

公开(公告)日：2017-12-28

申请号：US15645710

申请日：2017-07-10

Applicant: Iris International, Inc.

Inventor： Bart J. Wanders ,  Gregory A. Farrell ,  Thomas H. Adams ,  Warren Groner ,  Xiaodong Zhao ,  Brett Jordan ,  Jack Cremins ,  Carol Quon

IPC: G01N15/14 ,  G01N21/53 ,  G01N33/80 ,  G01N33/49 ,  G01N33/50 ,  G01N1/30 ,  G01N21/05 ,  G01N15/00 ,  G01N15/10

CPC classification number: G01N15/1404 ,  G01N1/30 ,  G01N15/1434 ,  G01N15/1463 ,  G01N15/1468 ,  G01N15/147 ,  G01N15/1475 ,  G01N21/53 ,  G01N33/49 ,  G01N33/4915 ,  G01N33/5091 ,  G01N33/5094 ,  G01N33/80 ,  G01N2015/0065 ,  G01N2015/1037 ,  G01N2015/1411 ,  G01N2015/1413 ,  G01N2015/1452 ,  G01N2015/1486 ,  G01N2021/058

Abstract: Aspects and embodiments of the instant disclosure provide a particle and/or intracellular organelle alignment agent for a particle analyzer used to analyze particles contained in a sample. An exemplary particle and/or intracellular organelle alignment agent includes an aqueous solution, a viscosity modifier, and/or a buffer. Embodiments also encompass systems, compositions, and methods for analyzing a sample containing particles. Parrticles such as blood cells can be categorized and counted by a digital image processor. A digital microscope camera can be directed, for example using certain focusing techniques, into a flowcell defining a symmetrically narrowing flowpath in which the sample stream flows in a ribbon flattened by flow and viscosity parameters between layers of sheath fluid. Blood cell images can be collected and analyzed using dynamic range extension processes and systems.

公开(公告)号：US09322752B2

公开(公告)日：2016-04-26

申请号：US14216533

申请日：2014-03-17

Applicant: IRIS International, Inc.

Inventor： Bart J. Wanders ,  Thomas H. Adams ,  Gregory A. Farrell ,  Warren Groner ,  Xiaodong Zhao

IPC: G01N33/00 ,  G01N31/00 ,  G01N1/30 ,  G01N33/50 ,  G01N33/80 ,  G01N21/53 ,  G01N15/14 ,  G01N15/00 ,  G01N15/10 ,  G01N21/05

CPC classification number: G01N15/1404 ,  G01N1/30 ,  G01N15/1434 ,  G01N15/1463 ,  G01N15/1468 ,  G01N15/147 ,  G01N15/1475 ,  G01N21/53 ,  G01N33/49 ,  G01N33/4915 ,  G01N33/5091 ,  G01N33/5094 ,  G01N33/80 ,  G01N2015/0065 ,  G01N2015/1037 ,  G01N2015/1411 ,  G01N2015/1413 ,  G01N2015/1452 ,  G01N2015/1486 ,  G01N2021/058

Abstract: The present disclosure relates to apparatus, systems, compositions, and methods for analyzing a sample containing particles. In some aspects the system comprises an analyzer which may be a visual analyzer. In one aspect, this disclosure relates to a particle imaging system comprising a flowcell through which a sample containing particles is caused to flow, and a high optical resolution imaging device which captures images for image analysis of samples. Other compositions, methods and features of this disclosure are disclosed herein.

Abstract translation: 本公开涉及用于分析含有颗粒的样品的装置，系统，组合物和方法。 在一些方面，该系统包括可以是视觉分析仪的分析器。 一方面，本发明涉及一种粒子成像系统，该粒子成像系统包括流过细胞，通过该流通池使含有颗粒的样品流动;以及高光学分辨率成像装置，其捕获用于样品的图像分析的图像。 本公开的其它组合物，方法和特征在此公开。

公开(公告)号：US20140315238A1

公开(公告)日：2014-10-23

申请号：US14215834

申请日：2014-03-17

Applicant: IRIS International, Inc.

Inventor： Gregory A. Farrell ,  Bart J. Wanders ,  Thomas H. Adams ,  Warren Groner ,  Xiaodong Zhao

IPC: G01N33/50

CPC classification number: G01N33/4915 ,  G01N15/1404 ,  G01N15/1434 ,  G01N15/1436 ,  G01N15/1459 ,  G01N15/1463 ,  G01N15/1468 ,  G01N15/147 ,  G01N15/1475 ,  G01N21/05 ,  G01N33/5091 ,  G01N2015/0065 ,  G01N2015/0073 ,  G01N2015/008 ,  G01N2015/0084 ,  G01N2015/1006 ,  G01N2015/1037 ,  G01N2015/1409 ,  G01N2015/1411 ,  G01N2015/1413 ,  G01N2015/1452 ,  G01N2015/1486 ,  G01N2021/058 ,  G01N2201/12

Abstract: Aspects and embodiments of the instant disclosure provide a particle and/or intracellular organelle alignment agent for a particle analyzer used to analyze particles contained in a sample. An exemplary particle and/or intracellular organelle alignment agent includes an aqueous solution, a viscosity modifier, and/or a buffer.

Abstract translation: 本公开的方面和实施方案提供用于分析样品中包含的颗粒的颗粒分析仪的颗粒和/或细胞内细胞器取向剂。 示例性的颗粒和/或细胞内细胞器取向剂包括水溶液，粘度调节剂和/或缓冲液。