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公开(公告)号：US11543340B2
公开(公告)日：2023-01-03
申请号：US16574693
申请日：2019-09-18
IPC分类号： G01N15/14 , G01N33/50 , G01N15/06 , G01N21/53 , G02B7/36 , G01N33/80 , G01N33/49 , G01N15/00 , G01N15/10 , G01N21/05 , G02B21/24 , G06T7/00 , G02B7/28 , G01N1/30
摘要： Particles such as blood cells can be categorized and counted by a digital image processor. A digital microscope camera can be directed into a flowcell defining a symmetrically narrowing flowpath in which the sample stream flows in a ribbon flattened by flow and viscosity parameters between layers of sheath fluid. A contrast pattern for autofocusing is provided on the flowcell, for example at an edge of a rear illumination opening. The image processor assesses focus accuracy from pixel data contrast. A positioning motor moves the microscope and/or flowcell along the optical axis for autofocusing on the contrast pattern target. The processor then displaces microscope and flowcell by a known distance between the contrast pattern and the sample stream, thus focusing on the sample stream. Blood cell images are collected from that position until autofocus is reinitiated, periodically, by input signal, or when detecting temperature changes or focus inaccuracy in the image data.
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公开(公告)号：US11525766B2
公开(公告)日：2022-12-13
申请号：US16533006
申请日：2019-08-06
发明人： Thomas H. Adams , Bart J. Wanders , John Roche , Harvey L. Kasdan
IPC分类号： G01N15/14 , G01N33/50 , G01N15/06 , G01N21/53 , G02B7/36 , G01N33/80 , G01N33/49 , G01N15/00 , G01N15/10 , G01N21/05 , G02B21/24 , G06T7/00 , G02B7/28 , G01N1/30
摘要： For analyzing a sample containing particles of at least two categories, such as a sample containing blood cells, a particle counter subject to a detection limit is coupled with an analyzer capable of discerning particle number ratios, such as a visual analyzer, and a processor. A first category of particles can be present beyond detection range limits while a second category of particles is present within respective detection range limits. The concentration of the second category of particles is determined by the particle counter. A ratio of counts of the first category to the second category is determined on the analyzer. The concentration of particles in the first category is calculated on the processor based on the ratio and the count or concentration of particles in the second category.
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3.发明授权公开(公告)号：US10794900B2
公开(公告)日：2020-10-06
申请号：US15819298
申请日：2017-11-21
发明人： Bart J. Wanders , Eric Chapoulaud , Brett Jordan
摘要： The present disclosure relates to apparatus, systems, compositions, and methods for analyzing a sample containing particles. A particle imaging system or analyzer can include a flowcell through which a urine sample containing particles is caused to flow, and a high optical resolution imaging device which captures images for image analysis. A contrast pattern for autofocusing is provided on the flowcell. The image processor assesses focus accuracy from pixel data contrast. A positioning motor moves the microscope and/or flowcell along the optical axis for autofocusing on the contrast pattern target. The processor then displaces microscope and flowcell by a known distance between the contrast pattern and the sample stream, thus focusing on the sample stream. Cell or particle images are collected from that position until autofocus is reinitiated, periodically, by input signal, or when detecting temperature changes or focus inaccuracy in the image data.
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公开(公告)号：US10705011B2
公开(公告)日：2020-07-07
申请号：US16336623
申请日：2017-10-05
发明人： Jiuliu Lu , Bian Qian , Bart J. Wanders , Ken Good , John Riley
摘要： A method for dynamic focusing is presented that can be performed by a dynamic focusing controller that can receive images from the image capture device, and for each image, determine a border of the particle within the image, and calculate a pixel intensity ratio of the image based on the border of the particle. The dynamic focusing controller can also calculate a median pixel intensity ratio from the pixel intensity ratios for each image, determine a focal distance direction based on the median pixel intensity ratio, calculate a focal distance based on the median pixel intensity ratio when the focal distance direction is positive, and calculate the focal distance based on a median border width when the focal distance direction is negative. The autofocusing controller can then send an instruction to the focusing mechanism to adjust the image capture device by the focal distance in the focal distance direction.
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公开(公告)号：US20160109372A1
公开(公告)日：2016-04-21
申请号：US14517421
申请日：2014-10-17
发明人： Bart J. Wanders
CPC分类号： G01N15/1463 , A61B5/0059 , G01N15/1404 , G01N15/1425 , G01N15/1459 , G01N21/05 , G01N21/85 , G01N2015/0065 , G01N2015/1409 , G01N2015/144
摘要： Systems and methods for imaging a plurality of blood fluid samples or other types of samples include processing at least a portion of a sample to enhance imageability of certain particles in that portion and subsequently imaging the sample portion. In some instances, processing and imaging of various samples may be staged in a manner to optimize throughput of the system or method.
摘要翻译： 用于对多个血液样品或其他类型的样品进行成像的系统和方法包括处理样品的至少一部分以增强该部分中某些颗粒的成像能力，随后对样品部分进行成像。 在一些情况下，可以以优化系统或方法的吞吐量的方式分级各种样品的处理和成像。
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6.发明申请公开(公告)号：US20140273068A1
公开(公告)日：2014-09-18
申请号：US14216811
申请日：2014-03-17
IPC分类号： G01N33/50
摘要： Particles such as blood cells can be categorized and counted by a digital image processor. A digital microscope camera can be directed into a flowcell defining a symmetrically narrowing flowpath in which the sample stream flows in a ribbon flattened by flow and viscosity parameters between layers of sheath fluid. A contrast pattern for autofocusing is provided on the flowcell, for example at an edge of a rear illumination opening. The image processor assesses focus accuracy from pixel data contrast. A positioning motor moves the microscope and/or flowcell along the optical axis for autofocusing on the contrast pattern target. The processor then displaces microscope and flowcell by a known distance between the contrast pattern and the sample stream, thus focusing on the sample stream. Blood cell images are collected from that position until autofocus is reinitiated, periodically, by input signal, or when detecting temperature changes or focus inaccuracy in the image data.
摘要翻译： 可以通过数字图像处理器对诸如血细胞的颗粒进行分类和计数。 数字显微镜照相机可以被引导到限定对称变窄的流动路径的流动池中，其中样品流在由鞘液层之间的流动和粘度参数平坦化的带中流动。 在流动池上提供用于自动聚焦的对比度图案，例如在后部照明开口的边缘。 图像处理器从像素数据对比度评估焦点精度。 定位电机沿着光轴移动显微镜和/或流动池，用于自动聚焦在对比度图案目标上。 然后，处理器将显微镜和流动池移位在对比度图案和样品流之间已知的距离，从而聚焦于样品流。 从该位置收集血细胞图像，直到通过输入信号周期性重新开始自动聚焦，或当检测图像数据中的温度变化或聚焦不准确时。
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公开(公告)号：US20230152203A1
公开(公告)日：2023-05-18
申请号：US17984116
申请日：2022-11-09
发明人： Thomas H. Adams , Bart J. Wanders , John Roche , Harvey L. Kasdan
CPC分类号： G01N15/1404 , G01N33/5094 , G01N15/06 , G01N15/1468 , G01N15/1434 , G01N15/147 , G01N15/1463 , G01N15/1475 , G01N21/53 , G01N15/1459 , G01N15/1429 , G01N15/1436 , G02B7/36 , G01N33/5091 , G01N33/80 , G01N1/30 , G01N33/49 , G01N33/4915 , G01N2015/1452 , G01N2015/0065
摘要： For analyzing a sample containing particles of at least two categories, such as a sample containing blood cells, a particle counter subject to a detection limit is coupled with an analyzer capable of discerning particle number ratios, such as a visual analyzer, and a processor. A first category of particles can be present beyond detection range limits while a second category of particles is present within respective detection range limits. The concentration of the second category of particles is determined by the particle counter. A ratio of counts of the first category to the second category is determined on the analyzer. The concentration of particles in the first category is calculated on the processor based on the ratio and the count or concentration of particles in the second category.
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公开(公告)号：US10451612B2
公开(公告)日：2019-10-22
申请号：US15263001
申请日：2016-09-12
摘要： Aspects and embodiments of the instant disclosure provide a particle and/or intracellular organelle alignment agent for a particle analyzer used to analyze particles contained in a sample. An exemplary particle and/or intracellular organelle alignment agent includes an aqueous solution, a viscosity modifier, and/or a buffer.
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9.发明申请公开(公告)号：US20180143182A1
公开(公告)日：2018-05-24
申请号：US15819298
申请日：2017-11-21
发明人： Bart J. Wanders , Eric Chapoulaud , Brett Jordan
摘要： The present disclosure relates to apparatus, systems, compositions, and methods for analyzing a sample containing particles. A particle imaging system or analyzer can include a flowcell through which a urine sample containing particles is caused to flow, and a high optical resolution imaging device which captures images for image analysis. A contrast pattern for autofocusing is provided on the flowcell. The image processor assesses focus accuracy from pixel data contrast. A positioning motor moves the microscope and/or flowcell along the optical axis for autofocusing on the contrast pattern target. The processor then displaces microscope and flowcell by a known distance between the contrast pattern and the sample stream, thus focusing on the sample stream. Cell or particle images are collected from that position until autofocus is reinitiated, periodically, by input signal, or when detecting temperature changes or focus inaccuracy in the image data.
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公开(公告)号：US20170370820A1
公开(公告)日：2017-12-28
申请号：US15645710
申请日：2017-07-10
发明人： Bart J. Wanders , Gregory A. Farrell , Thomas H. Adams , Warren Groner , Xiaodong Zhao , Brett Jordan , Jack Cremins , Carol Quon
IPC分类号： G01N15/14 , G01N21/53 , G01N33/80 , G01N33/49 , G01N33/50 , G01N1/30 , G01N21/05 , G01N15/00 , G01N15/10
CPC分类号： G01N15/1404 , G01N1/30 , G01N15/1434 , G01N15/1463 , G01N15/1468 , G01N15/147 , G01N15/1475 , G01N21/53 , G01N33/49 , G01N33/4915 , G01N33/5091 , G01N33/5094 , G01N33/80 , G01N2015/0065 , G01N2015/1037 , G01N2015/1411 , G01N2015/1413 , G01N2015/1452 , G01N2015/1486 , G01N2021/058
摘要： Aspects and embodiments of the instant disclosure provide a particle and/or intracellular organelle alignment agent for a particle analyzer used to analyze particles contained in a sample. An exemplary particle and/or intracellular organelle alignment agent includes an aqueous solution, a viscosity modifier, and/or a buffer. Embodiments also encompass systems, compositions, and methods for analyzing a sample containing particles. Parrticles such as blood cells can be categorized and counted by a digital image processor. A digital microscope camera can be directed, for example using certain focusing techniques, into a flowcell defining a symmetrically narrowing flowpath in which the sample stream flows in a ribbon flattened by flow and viscosity parameters between layers of sheath fluid. Blood cell images can be collected and analyzed using dynamic range extension processes and systems.
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