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公开(公告)号：US07183061B2
公开(公告)日：2007-02-27
申请号：US10606060
申请日：2003-06-25
IPC分类号： G01N33/53
CPC分类号： C12N15/8237 , C07K14/43563 , C07K14/721 , C07K2319/00 , C12N15/8238
摘要： The invention relates to an insect steroid receptor protein which is capable of acting as a gene switch which is responsive to a chemical inducer enabling external control of the gene.
摘要翻译： 本发明涉及昆虫类固醇受体蛋白质，其能够作为对能够对基因进行外部控制的化学诱导剂有反应的基因开关。
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公开(公告)号：US06610828B1
公开(公告)日：2003-08-26
申请号：US09564418
申请日：2000-05-03
IPC分类号： C07K100
CPC分类号： C12N15/8237 , C07K14/43563 , C07K14/721 , C07K2319/00 , C12N15/8238
摘要： The invention relates to an insect steroid receptor protein which is capable of acting as a gene switch which is responsive to a chemical inducer enabling external control of the gene.
摘要翻译： 本发明涉及昆虫类固醇受体蛋白质，其能够作为对能够对基因进行外部控制的化学诱导剂有反应的基因开关。
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公开(公告)号：US06379945B1
公开(公告)日：2002-04-30
申请号：US08653648
申请日：1996-05-24
IPC分类号： C12N510
CPC分类号： C07K14/721 , A61K38/00 , C07K2319/00 , C12N15/8237 , C12N15/8238
摘要： The invention relates to an insect steroid receptor protein which is capable of acting as a gene switch which is responsive to a chemical inducer enabling external control of the gene.
摘要翻译： 本发明涉及昆虫类固醇受体蛋白质，其能够作为对能够对基因进行外部控制的化学诱导剂有反应的基因开关。
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公开(公告)号：US5965387A
公开(公告)日：1999-10-12
申请号：US718751
申请日：1996-09-23
CPC分类号： C12N15/8238
摘要： A chemically inducible gene promoter sequence, and particularly, but not exclusively, a chemically inducible gene promoter sequence based on cis regulatory elements from the maize glutathione S-transferase 27 (GST-27) gene. In a preferred embodiment, the promoter sequence is operatively linked or fused to a gene or series of genes whereby expression of the gene or series of genes may be controlled by application of an effective exogenous inducer.
摘要翻译： 基于来自玉米谷胱甘肽S-转移酶27（GST-27）基因的顺式调节元件的化学诱导型基因启动子序列，特别地但不排他地是基于顺式调节元件的化学诱导型基因启动子序列。 在优选的实施方案中，启动子序列可操作地连接或融合到基因或一系列基因，从而通过施用有效的外源性诱导物来控制基因或一系列基因的表达。
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公开(公告)号：US06228643B1
公开(公告)日：2001-05-08
申请号：US08821994
申请日：1997-03-22
IPC分类号： C12N514
CPC分类号： C12N15/8237 , C12N9/50 , C12N15/8234 , C12N15/8238 , C12N15/8261 , Y02A40/146
摘要： A promoter comprising the DNA sequence of an oil seed rape cysteine protease gene promoter of class 1, 2 or 6 is described. The promoter may be used in an expression system for at least the tissue or tissues of a germinating seedling or developing grain or plant (e.g. in the root, cotyledons, leaves and stem). In a preferred embodiment, the expression system comprises a disrupter gene fused to a promoter according to the present invention.
摘要翻译： 描述了包含1,2或6类油菜半胱氨酸蛋白酶基因启动子的DNA序列的启动子。 启动子可以用于表达系统中至少用于发芽幼苗或发育中的谷物或植物（例如在根，子叶，叶和茎中）的组织或组织。 在优选的实施方案中，表达系统包含与根据本发明的启动子融合的破坏子基因。
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公开(公告)号：US6066456A
公开(公告)日：2000-05-23
申请号：US49289
申请日：1998-03-26
申请人： Ian George Bridges , Simon William Jonathan Bright , Andrew James Greenland , David Charles Holt , Ian Jepson , Wolfgang Walter Schuch
发明人： Ian George Bridges , Simon William Jonathan Bright , Andrew James Greenland , David Charles Holt , Ian Jepson , Wolfgang Walter Schuch
CPC分类号： C12N9/1088 , C12N15/8274
摘要： The chemically-inducible 27 kD subunit of the enzyme glutathione-S-transferase, isoform II (GST-II-27) and sequences encoding it are provided. In particular, a genomic DNA sequence encoding the gene promoter for the GST-II-27 subunit is provided. Then linked to an exogenous gene and introduced into a plant by transformation, and GST-II-27 promoter provides a means for the external regulation of expression of that exogenous gene. Transformation with DNA encoding glutathione-S-transferase polypeptides produces herbicide resistance transgenic plants.
摘要翻译： 提供酶谷胱甘肽-S-转移酶，同种型II（GST-II-27）的化学诱导型27kD亚基和编码它的序列。 特别地，提供了编码GST-II-27亚基的基因启动子的基因组DNA序列。 然后与外源基因连接并通过转化引入植物，GST-II-27启动子为外源基因的表达提供了一种手段。 用编码谷胱甘肽-S-转移酶多肽的DNA转化产生除草剂抗性转基因植物。
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公开(公告)号：US5866792A
公开(公告)日：1999-02-02
申请号：US664855
申请日：1996-06-17
申请人： Ian George Bridges , Simon William Jonathan Bright , Andrew James Greenland , David Charles Holt , Ian Jepson , Wolfgang Walter Schuch
发明人： Ian George Bridges , Simon William Jonathan Bright , Andrew James Greenland , David Charles Holt , Ian Jepson , Wolfgang Walter Schuch
IPC分类号： A01H5/00 , C12N5/10 , C12N9/10 , C12N15/09 , C12N15/29 , C12N15/54 , C12N15/82 , A01H5/10 , C12N15/52 , C12N15/85
CPC分类号： C12N9/1088 , C12N15/8238 , C12N15/8274
摘要： The chemically-inducible 27 kD subunit of the enzyme glutathione-S-transferase, isoform II (GST-II-27) and sequences encoding it are provided. In particular, a genomic DNA sequence encoding the gene promoter for the GST-II-27 subunit is provided. Then linked to an exogenous gene and introduced into a plant by transformation, and GST-II-27 promoter provides a means for the external regulation of expression of that exogenous gene. Transformation with DNA encoding glutathione-S-transferase polypeptides produces herbicide resistance transgenic plants.
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公开(公告)号：US5808034A
公开(公告)日：1998-09-15
申请号：US293422
申请日：1994-08-22
申请人： Ian George Bridges , Simon William Jonathan Bright , Andrew James Greenland , Wolfgang Walter Schuch
发明人： Ian George Bridges , Simon William Jonathan Bright , Andrew James Greenland , Wolfgang Walter Schuch
IPC分类号： A01H5/00 , C07K14/005 , C07K14/415 , C07K14/47 , C12N1/19 , C12N5/10 , C12N9/10 , C12N9/88 , C12N15/09 , C12N15/10 , C12N15/64 , C12N15/73 , C12N15/81 , C12N15/82 , C12P21/02 , C12Q1/68 , C12R1/01 , C12N15/11 , A01H1/00
CPC分类号： C12N9/1088 , C07K14/005 , C07K14/415 , C07K14/4703 , C12N15/10 , C12N15/64 , C12N15/73 , C12N15/81 , C12N15/8216 , C12N15/8217 , C12N15/8222 , C12N15/8231 , C12N15/8237 , C12N15/8238 , C12N15/8289 , C12N9/88 , C07K2319/00 , C12N2795/10022 , Y10S47/01
摘要： Male sterility is imparted to a plant by a cascade of gene sequences which expresses a protein which disrupts the biosynthesis of viable pollen. Expression of the disrupter protein is restricted to male parts of the plant by an upstream promoter sequence which is specific to male flowers, the male specific promoter being under control of an operator sequence. The cascade also includes a gene encoding a repressor protein specific for that operator. Expression of the repressor protein is under control of a chemically inducible promoter which is inducible by the application to the plant by, spraying or like process, of an exogenous chemical. In the absence of the exogenous chemical inducer, no repressor protein is expressed, resulting in expression of the disrupter protein and, consequently, male sterility. Fertility may be restored to the plant, when required for maintenance of the line, by spraying with the inducer, resulting in expression of the repressor which binds the operator and inhibits expression of the disrupter protein.
摘要翻译： 通过表达破坏活花粉生物合成的蛋白质的基因序列的级联赋予植物雄性不育。 破坏蛋白的表达通过对雄性花特异性的上游启动子序列限制在植物的雄性部分，雄性特异性启动子由操作者序列控制。 级联还包括编码针对该操作者特异性的阻遏物蛋白的基因。 抑制蛋白的表达受化学诱导型启动子的控制，其可通过外源化学品的喷雾或类似方法施用于植物而诱导。 在没有外源性化学诱导剂的情况下，没有表达阻抑蛋白，导致破坏蛋白的表达，从而导致雄性不育。 生殖力可以恢复到植物，当需要维持生产线时，通过喷洒诱导剂，导致阻遏物的表达，其结合操作者并抑制破坏蛋白的表达。
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公开(公告)号：US6037524A
公开(公告)日：2000-03-14
申请号：US930894
申请日：1997-10-09
IPC分类号： A01H5/00 , C12N5/10 , C12N9/14 , C12N15/00 , C12N15/09 , C12N15/29 , C12N15/55 , C12N15/82 , C12N15/84 , C12R1/91
CPC分类号： C12N15/8216 , C12N15/8243 , C12N15/8282 , C12N9/14
摘要： A promoter derived from an SHH gene, especially the SHH gene of Arabidopsis thaliana which is capable of directing expression on a variety of operator genes in both monocotyledonous and dicotyledonous plants. The promoter of the invention may be used for directing expression of pathogen resistance genes to disease or wound sites.
摘要翻译： PCT No.PCT / GB96 / 00882 Sec。 371 1997年10月9日第 102（e）日期1997年10月9日PCT 1996年4月10日PCT PCT。 WO96 / 32488 PCT公开号 日期1996年10月17日来自SHH基因的启动子，特别是拟南芥的SHH基因，其能够指导在单子叶植物和双子叶植物中的多种运算子基因的表达。 本发明的启动子可用于将病原体抗性基因的表达指导到疾病或伤口部位。
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公开(公告)号：US06599514B1
公开(公告)日：2003-07-29
申请号：US09446472
申请日：1999-12-21
IPC分类号： A01N2534
CPC分类号： A23L3/3571 , A01N65/00 , A01N65/08 , A01N65/12 , A01N65/20 , A01N65/44 , A23L3/3472 , A23L3/3508 , A23L3/3526 , A01N31/08 , A01N31/16 , A01N37/06 , A01N37/10 , A01N37/36 , A01N37/40 , A01N37/44 , A01N61/00
摘要： Antifungal compositions having enhanced antifungal activity comprising an antifungal agent and a food additive are described. Also described are a method for inhibiting fungal growth using the compositions with enhanced activity and the use of a food additive to enhance the antifungal properties of an antifungal agent.
摘要翻译： 描述了具有增强的抗真菌活性的抗真菌组合物，其包含抗真菌剂和食品添加剂。 还描述了使用具有增强的活性的组合物以及使用食品添加剂来增强抗真菌剂的抗真菌性质来抑制真菌生长的方法。
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