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    Plant gene construct encoding a protein capable of disrupting the biogenesis of viable pollen
    3.
    发明授权
    Plant gene construct encoding a protein capable of disrupting the biogenesis of viable pollen 失效
    编码能够破坏活花粉生物发生的蛋白质的植物基因构建体

    公开(公告)号：US06172279B2

    公开(公告)日：2001-01-09

    申请号：US08554811

    申请日：1995-11-07

    申请人： Ian George Bridges ,  Simon William Jonathan Bright ,  Andrew James Greenland ,  Wolfgang Walter Schuch

    发明人： Ian George Bridges ,  Simon William Jonathan Bright ,  Andrew James Greenland ,  Wolfgang Walter Schuch

    IPC分类号： C12N1511

    CPC分类号： C12N9/1088 ,  C07K14/005 ,  C07K14/415 ,  C07K14/4703 ,  C07K2319/00 ,  C12N9/88 ,  C12N15/10 ,  C12N15/64 ,  C12N15/73 ,  C12N15/81 ,  C12N15/8216 ,  C12N15/8217 ,  C12N15/8222 ,  C12N15/8231 ,  C12N15/8237 ,  C12N15/8238 ,  C12N15/8289 ,  C12N2795/10022 ,  Y10S47/01

    摘要： Male sterility is imparted to a plant by a cascade of gene sequences which expresses a protein which disrupts the biosynthesis of viable pollen. Expression of the disrupter protein is restricted to male parts of the plant by an upstream promoter sequence which is specific to male flowers, the male specific promoter being under control of an operator sequence. The cascade also includes a gene encoding a repressor protein specific for that operator. Expression of the repressor protein is under control of a chemically inducible promoter which is inducible by the application to the plant by, spraying or like process, of an exogenous chemical. In the absence of the exogenous chemical inducer, no repressor protein is expressed, resulting in expression of the disrupter protein and, consequently, male sterility. Fertility may be restored to the plant, when required for maintenance of the line, by spraying with the inducer, resulting in expression of the repressor which binds the operator and inhibits expression of the disrupter protein.

    摘要翻译： 通过表达破坏活花粉生物合成的蛋白质的基因序列的级联赋予植物雄性不育。 破坏蛋白的表达通过对雄性花特异性的上游启动子序列限制在植物的雄性部分，雄性特异性启动子由操作者序列控制。 级联还包括编码针对该操作者特异性的阻遏物蛋白的基因。 抑制蛋白的表达受化学诱导型启动子的控制，其可通过外源化学品的喷雾或类似方法施用于植物而诱导。 在没有外源性化学诱导剂的情况下，没有表达阻抑蛋白，导致破坏蛋白的表达，从而导致雄性不育。 生殖力可以恢复到植物，当需要维持生产线时，通过喷洒诱导剂，导致阻遏物的表达，其结合操作者并抑制破坏蛋白的表达。