公开(公告)号：US09023384B2

公开(公告)日：2015-05-05

申请号：US11666736

申请日：2005-11-02

申请人： Ikuo Morita ,  Kazunari Akiyoshi ,  Shinichiro Nomura

发明人： Ikuo Morita ,  Kazunari Akiyoshi ,  Shinichiro Nomura

IPC分类号： A61K9/127 ,  A61K47/42 ,  C12N5/00 ,  A61K38/17

CPC分类号： A61K38/177 ,  A61K9/127

摘要： It is intended to efficiently inject into a target cell, a substance charged within a liposome. The present inventors have found that connexin synthesized within a liposome is introduced as connexon having a gap junction function into the liposome membrane. Specifically, the liposome according to the present invention is a liposome in which connexon composed of connexin synthesized by an in-vitro protein synthesis system is incorporated in a state of having a gap junction function.

摘要翻译： 旨在有效地注射到靶细胞中，该细胞是置于脂质体内的物质。 本发明人已经发现，将在脂质体内合成的连接蛋白作为具有间隙连接功能的连接子引入脂质体膜。 具体地说，本发明的脂质体是脂质体，其中通过体外蛋白质合成系统合成的由连接蛋白组成的连接体以具有间隙连接功能的状态结合。

公开(公告)号：US20080279918A1

公开(公告)日：2008-11-13

申请号：US11666736

申请日：2005-11-02

申请人： Ikuo Morita ,  Kazunari Akiyoshi ,  Shinichiro Nomura

发明人： Ikuo Morita ,  Kazunari Akiyoshi ,  Shinichiro Nomura

IPC分类号： A61K9/127 ,  A61K47/42 ,  C12N5/00

CPC分类号： A61K38/177 ,  A61K9/127

摘要： It is intended to efficiently inject into a target cell, a substance charged within a liposome. The present inventors have found that connexin synthesized within a liposome is introduced as connexon having a gap junction function into the liposome membrane. Specifically, the liposome according to the present invention is a liposome in which connexon composed of connexin synthesized by an in-vitro protein synthesis system is incorporated in a state of having a gap junction function.

摘要翻译： 旨在有效地注射到靶细胞中，该细胞是置于脂质体内的物质。 本发明人已经发现，将在脂质体内合成的连接蛋白作为具有间隙连接功能的连接子引入脂质体膜。 具体地说，本发明的脂质体是脂质体，其中通过体外蛋白质合成系统合成的由连接蛋白组成的连接体以具有间隙连接功能的状态结合。

公开(公告)号：US5326785A

公开(公告)日：1994-07-05

申请号：US040604

申请日：1993-03-31

申请人： Hidetsura Cho ,  Mie Tamaoka ,  Seiitsu Murota ,  Ikuo Morita

发明人： Hidetsura Cho ,  Mie Tamaoka ,  Seiitsu Murota ,  Ikuo Morita

IPC分类号： A61K31/275 ,  A61K31/34 ,  A61K31/341 ,  A61K31/38 ,  A61K31/381 ,  A61K31/415 ,  A61K31/44 ,  A61K31/4402 ,  A61K31/4406 ,  A61K31/4418 ,  A61P9/00 ,  A61P9/10 ,  C07C255/23 ,  C07C255/41 ,  C07C271/44 ,  C07C323/16 ,  C07C323/18 ,  C07D213/30 ,  C07D233/60 ,  C07D307/42 ,  C07D333/16 ,  C07D521/00 ,  C07D317/08 ,  A61K31/21

CPC分类号： C07D213/30 ,  C07C255/23 ,  C07D231/12 ,  C07D233/56 ,  C07D249/08 ,  C07D307/42 ,  C07D333/16

摘要： Described are caffeic acid derivatives represented by the following formula: ##STR1## wherein either one of R.sup.1 and R.sup.2 is a hydrogen atom, a C.sub.1 -C.sub.6 alkyl group, a group --CO.sub.2 R.sup.4, or a group --CONR.sup.5 R.sup.6, R.sup.4, R.sup.5 and R.sup.6 being either a hydrogen atom or a particular group, and the other is the group --CO.sub.2 R.sup.4 or the group --CONR.sup.5 R.sup.6, or R.sup.1 and R.sup.2 are coupled together to represent a 5-membered ring so formed, Y represents a particular group or atom, X represents a substituted or unsubstituted C.sub.6 -C.sub.10 aryl, C.sub.7 -C.sub.12 aralkyl, heterocyclic or heterocyclic ring-alkyl group, m and n stand for particular integers, and R.sup.3 represents a hydrogen atom, a hydroxyl group, a group --OCO.sub.2 R.sup.7, R.sup.7 being a hydrogen atom or a particular group, or a group --OCONR.sup.8 R.sup.9, R.sup.8 representing a C.sub.1 -C.sub.6 alkyl group and R.sup.9 representing a hydrogen atom or a C.sub.1 -C.sub.6 alkyl group, and pharmaceutically acceptable salts thereof. Therapeutic agents containing the derivatives or salts are effective for diseases of the circulatory system.

摘要翻译： 描述的是由下式表示的咖啡酸衍生物：其中R 1和R 2中的任一个是氢原子，C 1 -C 6烷基，-CO 2 R 4基或-CONR 5 R 6基，R 4，R 5和R 6是 另一个是-CO 2 R 4或-CONR 5 R 6基团，或者R 1和R 2连接在一起以表示如此形成的5元环，Y表示特定的基团或原子，X表示 取代或未取代的C 6 -C 10芳基，C 7 -C 12芳烷基，杂环或杂环环烷基，m和n表示特定整数，R 3表示氢原子，羟基，-OCO 2 R 7基，R 7为氢 或基团-OCONR 8 R 9，R 8表示C 1 -C 6烷基，R 9表示氢原子或C 1 -C 6烷基，及其药学上可接受的盐。 含有衍生物或盐的治疗剂对于循环系统的疾病是有效的。

公开(公告)号：US08367411B2

公开(公告)日：2013-02-05

申请号：US11597975

申请日：2005-05-31

申请人： Ikuo Morita ,  Hideyuki Miyake

发明人： Ikuo Morita ,  Hideyuki Miyake

IPC分类号： C12N5/07

CPC分类号： C12N5/069 ,  A61F2/06 ,  A61L27/507 ,  C12N5/0068 ,  C12N5/0691 ,  C12N2535/10

摘要： An artificial tissue capable of carrying necessary nutrients for maintaining activities of cells and tissues, and a method of manufacturing an artificial blood vessel. A plurality of forms of blood vessels are extracted from an image of a living tissue and made into a blood vessel form image. Each of the blood vessel forms of the blood vessel form image is adjusted and a blood vessel formation pattern is formed. A blood vessel cell culturing pattern of forming is formed, in a cell culturing layer. The blood vessel cell culturing pattern includes: a cell adhesion portion having adhesive properties with a blood vessel cell and formed to the blood vessel formation pattern; and a cell adhesion-inhibiting portion having cell adhesion-inhibiting properties for inhibiting adhesion with a blood vessel cell and formed in an area other than the cell adhesion portion. A blood vessel cell is adhered to the cell adhesion portion, and cultured into a tissue.

摘要翻译： 能够携带用于维持细胞和组织的活性的必需营养物的人造组织，以及制造人造血管的方法。 从活体组织的图像中提取多个形式的血管，并制成血管形成图像。 调整血管形成图像的血管形式，形成血管形成图案。 在细胞培养层中形成血管细胞培养图案。 血管细胞培养图案包括：细胞粘附部，其与血管细胞具有粘附性，形成于血管形成图案; 以及细胞粘附抑制部，其具有抑制与血管细胞粘附并形成于细胞粘附部以外的区域的细胞粘附抑制性。 将血管细胞粘附到细胞粘附部分，并培养成组织。

公开(公告)号：US20120040571A1

公开(公告)日：2012-02-16

申请号：US13263365

申请日：2010-06-18

申请人： Nobuaki Yoshioka ,  Ikuo Morita

发明人： Nobuaki Yoshioka ,  Ikuo Morita

IPC分类号： H01R13/405 ,  H01R13/52

CPC分类号： H01R13/504 ,  H01R43/24

摘要： There is provided a method of integrally molding a connector in which the number of components can be reduced, and productivity can be enhanced.The method of integrally molding a connector according to the invention includes a first step of injection molding elastic resin 15 around a connection part 9 and an end part of a cover part 5 of an electric wire which is positioned adjacent to the connection part 9 thereby to bond the cover part 5 and the elastic resin 15 to each other, and a second step of injection molding resin 17 around the elastic resin 15 so as to compress the elastic resin 15 thereby to press-fit the elastic resin 15 to a terminal 11, and at the same time, to bond the elastic resin 15 and the resin 17 to each other.

摘要翻译： 提供了一种整体模制连接器的方法，其中可以减少部件数量，并且可以提高生产率。 根据本发明的一体模制连接器的方法包括：将邻近连接部分9定位的电线的连接部9和盖部分5的端部周围的弹性树脂15注塑成型的第一步骤， 将盖部5和弹性树脂15彼此接合，并且在弹性树脂15周围注塑树脂17的第二步骤，以压缩弹性树脂15，从而将弹性树脂15压配合到端子11， 并且同时将弹性树脂15和树脂17彼此粘合。

公开(公告)号：US20080085544A1

公开(公告)日：2008-04-10

申请号：US11597975

申请日：2005-05-31

申请人： Ikuo Morita ,  Hideyuki Miyake

发明人： Ikuo Morita ,  Hideyuki Miyake

IPC分类号： C12N13/00 ,  C12N5/00 ,  C12N5/02

CPC分类号： C12N5/069 ,  A61F2/06 ,  A61L27/507 ,  C12N5/0068 ,  C12N5/0691 ,  C12N2535/10

摘要： An artificial tissue capable of carrying necessary nutrients for maintaining activities of cells and tissues, and a method of manufacturing an artificial blood vessel. A plurality of forms of blood vessels are extracted from an image of a living tissue and made into a blood vessel form image. Each of the blood vessel forms of the blood vessel form image is adjusted and a blood vessel formation pattern is formed. A blood vessel cell culturing pattern of forming is formed, in a cell culturing layer. The blood vessel cell culturing pattern includes: a cell adhesion portion having adhesive properties with a blood vessel cell and formed to the blood vessel formation pattern; and a cell adhesion-inhibiting portion having cell adhesion-inhibiting properties for inhibiting adhesion with a blood vessel cell and formed in an area other than the cell adhesion portion. A blood vessel cell is adhered to the cell adhesion portion, and cultured into a tissue.

摘要翻译： 能够携带用于维持细胞和组织的活性的必需营养物的人造组织，以及制造人造血管的方法。 从活体组织的图像中提取多个形式的血管，并制成血管形成图像。 调整血管形成图像的血管形式，形成血管形成图案。 在细胞培养层中形成血管细胞培养图案。 血管细胞培养图案包括：细胞粘附部，其与血管细胞具有粘附性，形成于血管形成图案; 以及细胞粘附抑制部，其具有抑制与血管细胞粘附并形成于细胞粘附部以外的区域的细胞粘附抑制性。 将血管细胞粘附到细胞粘附部分，并培养成组织。

公开(公告)号：US20070122901A1

公开(公告)日：2007-05-31

申请号：US10574687

申请日：2004-10-15

申请人： Ikuo Morita ,  Makoto Nakamura ,  Hideyuki Miyake ,  Hideshi Hattori ,  Hironori Kobayashi ,  Masaaki Kurihara

发明人： Ikuo Morita ,  Makoto Nakamura ,  Hideyuki Miyake ,  Hideshi Hattori ,  Hironori Kobayashi ,  Masaaki Kurihara

IPC分类号： C12N5/06

CPC分类号： C12N5/0068 ,  A61L27/38 ,  C12N2533/00

摘要： The present invention relates to a method for culturing cells, which comprises the steps of: causing cells to adhere to the surface of a cell array substrate having a cell adhesiveness variation pattern that comprises regions having good cell adhesiveness and regions having inhibited cell adhesiveness patterned on a substrate; transferring the adhered cells to a cell culture substrate in such patterned state; and culturing the transferred cells.

摘要翻译： 本发明涉及一种培养细胞的方法，包括以下步骤：使细胞粘附到具有细胞粘附性变化图案的细胞阵列基板的表面，所述细胞粘附变化图案包含具有良好细胞粘附性的区域和图案化的细胞粘附性抑制区域 底物; 以这样的图案化状态将粘附的细胞转移到细胞培养基质; 并培养转移的细胞。

公开(公告)号：US5232941A

公开(公告)日：1993-08-03

申请号：US750396

申请日：1991-08-27

申请人： Hidetsura Cho ,  Mie Tamaoka ,  Seiitsu Murota ,  Ikuo Morita

发明人： Hidetsura Cho ,  Mie Tamaoka ,  Seiitsu Murota ,  Ikuo Morita

IPC分类号： A61K31/275 ,  A61K31/34 ,  A61K31/341 ,  A61K31/38 ,  A61K31/381 ,  A61K31/415 ,  A61K31/44 ,  A61K31/4402 ,  A61K31/4406 ,  A61K31/4418 ,  A61P9/00 ,  A61P9/10 ,  C07C255/23 ,  C07C255/41 ,  C07C271/44 ,  C07C323/16 ,  C07C323/18 ,  C07D213/30 ,  C07D233/60 ,  C07D307/42 ,  C07D333/16 ,  C07D521/00

CPC分类号： C07D213/30 ,  C07C255/23 ,  C07D231/12 ,  C07D233/56 ,  C07D249/08 ,  C07D307/42 ,  C07D333/16

摘要： Described are caffeic acid derivatives represented by the following formula: ##STR1## wherein either one of R.sup.1 and R.sup.2 is a hydrogen atom, a C.sub.1 -C.sub.6 alkyl group, a group --CO.sub.2 R.sup.4, or a group --CONR.sup.5 R.sup.6, R.sup.4, R.sup.5 and R.sup.6 being either a hydrogen atom or a particular group, and the other is the group --CO.sub.2 R.sub.4 or the group --CONF.sup.5 R.sup.6, or R.sup.1 and R.sup.2 are coupled together to represent a 5-membered ring so formed, Y represents a particular group or atom, X represents a substituted or unsubstituted C.sub.6 -C.sub.10 aryl, C.sub.7 -C.sub.12 aralkyl, heterocyclic or heterocyclic ring-alkyl group, m and n stand for particular integers, and R.sub.3 represents a hydrogen atom, a hydroxyl group, a group --OCO.sub.2 R.sup.7, R.sup.7 being a hydrogen atom or a particular group, or a group --OCONR.sup.8 R.sup.9, R.sup.8 representing a C.sub.1 -C.sub.6 alkyl group and R.sup.9 representing a hydrogen atom or a C.sub.1 -C.sub.6 alkyl group, and pharmaceutically acceptable salts thereof. Therapeutic agents containing the derivatives or salts are effective for diseases of the circulatory system.

公开(公告)号：US08851935B2

公开(公告)日：2014-10-07

申请号：US13395908

申请日：2011-01-24

申请人： Nobuaki Yoshioka ,  Yasuhiro Yamaguchi ,  Ikuo Morita

发明人： Nobuaki Yoshioka ,  Yasuhiro Yamaguchi ,  Ikuo Morita

IPC分类号： H01R13/04 ,  H01R13/405 ,  H01R4/02 ,  H01R24/28 ,  H01R105/00

CPC分类号： H01R13/405 ,  H01R4/023 ,  H01R24/28 ,  H01R2105/00

摘要： A connector includes a plurality of terminal metal fittings that are arranged in parallel to one another and respectively have electric wire connection sections to which electric wires are electrically connected, and a connector housing that accommodates the plurality of terminal metal fittings therein. The plurality of terminal metal fittings are configured that the lengths of the electric wire connection sections are made longer in accordance with the arrangement thereof from one side toward the other side in an arrangement direction of the plurality of terminal metal fittings. The electric wires are connected to the electric wire connection sections respectively so that the electric wires extend from one end portions of the electric wire connection sections toward the one side and intersect with a longitudinal direction of the electric wire connection section.

摘要翻译： 连接器包括多个端子金属配件，它们彼此平行地布置并且分别具有电线连接的电线连接部分和容纳多个端子金属配件的连接器壳体。 多个端子金属配件被构造成使得电线连接部分的长度根据其在多个端子金属配件的排列方向上的一侧朝向另一侧的布置而更长。 电线分别连接到电线连接部分，使得电线从电线连接部分的一个端部朝向一侧延伸，并与电线连接部分的纵向方向相交。

公开(公告)号：US08556422B2

公开(公告)日：2013-10-15

申请号：US13071136

申请日：2011-03-24

申请人： Takuya Kishimoto ,  Sakuya Tamada ,  Akio Yasuda ,  Kyoko Ohno ,  Ikuo Morita

发明人： Takuya Kishimoto ,  Sakuya Tamada ,  Akio Yasuda ,  Kyoko Ohno ,  Ikuo Morita

IPC分类号： A61B3/14 ,  A61B3/00

CPC分类号： A61B3/1225 ,  A61B3/1015

摘要： A fluorescence image acquisition method including: irradiating an eyeground with a short-pulse beam of light for exciting a fluorescent dye; setting the time point of emission of the light as a reference, measuring the intensities of luminescence of the fluorescent dye at two different times which are predetermined periods of time after the reference, determining the ratio between the intensities of luminescence at the two different times, and detecting the intensity of luminescence of the fluorescent dye having marked a target by using the ratio; and generating a fluorescence image of the fluorescent dye having marked the target, based on the results of detection by the detecting step.

摘要翻译： 一种荧光图像获取方法，包括：用短脉冲光束照射眼睛以激发荧光染料; 将发光的时间点设定为参考，测量在参考后预定时间段的两个不同时间的荧光染料的发光强度，确定两个不同时间的发光强度之间的比率， 并通过使用该比例来检测标记有靶标的荧光染料的发光强度; 以及基于检测步骤的检测结果产生标记了目标物的荧光染料的荧光图像。