公开(公告)号：US11788139B2

公开(公告)日：2023-10-17

申请号：US17307959

申请日：2021-05-04

申请人： Illumina, Inc.

发明人： Ryan Kelley ,  Gordon Bean ,  Eric Vermaas

IPC分类号： C12Q1/6874 ,  G16B30/00 ,  C12N15/10 ,  C40B40/06 ,  C12Q1/6811 ,  C12Q1/6869 ,  G16B30/10

CPC分类号： C12Q1/6874 ,  C12N15/1065 ,  C12Q1/6811 ,  C12Q1/6869 ,  C40B40/06 ,  G16B30/00 ,  G16B30/10 ,  C12Q1/6869 ,  C12Q2525/191 ,  C12Q2535/122 ,  C12Q2537/143 ,  C12Q2563/179

摘要： The disclosed embodiments concern index oligonucleotides configured to identify sources of samples of nucleic acids and methods, apparatus, systems and computer program products for identifying and making the index oligonucleotides. In some implementations, the index oligonucleotides include a set of index sequences, a Hamming distance between any two index sequences of the set of index sequences meeting one or more criteria. System, apparatus, and computer program products are also provided for determining a sequence of interest using the index oligonucleotides.

公开(公告)号：US20210230689A1

公开(公告)日：2021-07-29

申请号：US17227639

申请日：2021-04-12

申请人： Illumina, Inc. ,  Illumina Cambridge Limited

发明人： Eric Vermaas ,  Mahdieh Khosroheidari ,  Angela Kalbande ,  Vincent Peter Smith ,  Claire Bevis-Mott ,  Jonathan Mark Boutell ,  Michael Chesney

IPC分类号： C12Q1/6874 ,  C12Q1/6809 ,  C12Q1/6816 ,  C12Q1/6834 ,  C12Q1/6855 ,  C12Q1/6806

摘要： The present invention is concerned with compositions and methods for improving the rate of correct sample identification in indexed nucleic acid library preparations for multiplex next generation sequencing by blocking the 3′ ends of pooled indexed polynucleotides from multiple samples prior to amplification and sequencing, by exonuclease treatment and optionally blocking the 3′ ends of pooled indexed polynucleotides from multiple samples prior to amplification and sequencing, by exonuclease treatment after protective adapters are ligated to target polynucleotides to degrade unincorporated adapters prior to amplification and sequencing, and/or by modifying or blocking 5′ and 3′ ends of pooled indexed polynucleotides from multiple samples, with an optional exonuclease treatment, prior to amplification and sequencing.

公开(公告)号：US11814678B2

公开(公告)日：2023-11-14

申请号：US17307973

申请日：2021-05-04

申请人： Illumina, Inc.

发明人： Tatjana Singer ,  Ryan Kelley ,  Gordon Bean ,  Eric Vermaas

IPC分类号： C12Q1/6874 ,  G16B25/00 ,  C12Q1/6869 ,  G16B30/00 ,  G16B30/10 ,  G16B25/20

CPC分类号： C12Q1/6874 ,  C12Q1/6869 ,  G16B25/00 ,  G16B30/00 ,  G16B30/10 ,  G16B25/20 ,  C12Q1/6869 ,  C12Q2525/191 ,  C12Q2535/122 ,  C12Q2537/143 ,  C12Q2563/179

摘要： The disclosed embodiments concern index oligonucleotides configured to identify sources of samples of nucleic acids and methods, apparatus, systems and computer program products for identifying and making the index oligonucleotides. In some implementations, the index oligonucleotides include a set of index sequences, a Hamming distance between any two index sequences of the set of index sequences meeting one or more criteria. System, apparatus, and computer program products are also provided for determining a sequence of interest using the index oligonucleotides.

公开(公告)号：US20220082569A1

公开(公告)日：2022-03-17

申请号：US17459459

申请日：2021-08-27

申请人： ILLUMINA, INC. ,  ILLUMINA SINGAPORE PTE. LTD.

发明人： Rigo Pantoja ,  Daniel Ortiz ,  Xiangyuan Yang ,  Yin Nah Teo ,  Eric Vermaas ,  Allen Eckhardt

IPC分类号： G01N33/68 ,  C12Q1/6869

摘要： A method is provided for detecting an element. The method includes coupling an element-sugar complex to a substrate. The element-sugar complex includes an element coupled to a sugar. The method includes coupling a lectin to the sugar; detecting the lectin; and detecting the element using at least the detection of the lectin. A system is provided that includes a substrate, and an element-sugar complex coupled to the substrate. The element-sugar complex includes an element coupled to a sugar. The system includes a lectin coupled to the sugar; and detection circuitry to detect the element using at least detection of the lectin.

公开(公告)号：US20160237489A1

公开(公告)日：2016-08-18

申请号：US15067013

申请日：2016-03-10

申请人： Illumina, Inc.

发明人： Min-Jui Richard Shen ,  Robert C. Kain ,  Kenneth M. Kuhn ,  AmirAli Hajhossein Talasaz ,  Arash Jamshidi ,  George Sakaldasis ,  Eric Vermaas ,  Sebastian Bohm ,  Tarun Khurana ,  Helmy A. Eltoukhy ,  Jian Gong

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6874 ,  B01L3/502792 ,  B01L2200/0673 ,  B01L2300/0636 ,  B01L2300/069 ,  B01L2300/0816 ,  B01L2300/0851 ,  B01L2300/165 ,  B01L2400/0427

摘要： Provided are methods and apparatuses for performing sequencing using droplet manipulation, for example, via electrowetting-based techniques. Also provided are integrated methods and apparatuses for performing sample preparation and sequencing on the same apparatus. In addition, provided are methods of reducing reagent waste and preloaded consumable cartridges comprising reagents for sample preparation and/or sequencing.

公开(公告)号：US09309571B2

公开(公告)日：2016-04-12

申请号：US14084355

申请日：2013-11-19

申请人： ILLUMINA, INC.

发明人： Min-Jui Richard Shen ,  Robert C. Kain ,  Kenneth M. Kuhn ,  AmirAli Hajhossein Talasaz ,  Arash Jamshidi ,  George Sakaldasis ,  Eric Vermaas ,  Sebastian Bohm ,  Tarun Khurana ,  Helmy A. Eltoukhy ,  Jian Gong

IPC分类号： C12Q1/68 ,  B81B7/00 ,  G01N27/26 ,  B01L3/00

CPC分类号： C12Q1/6874 ,  B01L3/502792 ,  B01L2200/0673 ,  B01L2300/0636 ,  B01L2300/069 ,  B01L2300/0816 ,  B01L2300/0851 ,  B01L2300/165 ,  B01L2400/0427

摘要： Provided are methods and apparatuses for performing sequencing using droplet manipulation, for example, via electrowetting-based techniques. Also provided are integrated methods and apparatuses for performing sample preparation and sequencing on the same apparatus. In addition, provided are methods of reducing reagent waste and preloaded consumable cartridges comprising reagents for sample preparation and/or sequencing.

摘要翻译： 提供了用于使用液滴操纵进行排序的方法和装置，例如通过基于电润湿的技术。 还提供了用于在同一装置上进行样品制备和测序的综合方法和装置。 此外，提供了减少试剂废物的方法和包括用于样品制备和/或测序的试剂的预装的消耗品筒。

公开(公告)号：US20140073514A1

公开(公告)日：2014-03-13

申请号：US14084355

申请日：2013-11-19

申请人： ILLUMINA, INC.

发明人： Min-Jui Richard Shen ,  Robert C. Kain ,  Kenneth M. Kuhn ,  AmirAli Hajhossein Talasaz ,  Arash Jamshidi ,  George Sakaldasis ,  Eric Vermaas ,  Sebastian Bohm ,  Tarun Khurana ,  Helmy A. Eltoukhy ,  Jian Gong

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6874 ,  B01L3/502792 ,  B01L2200/0673 ,  B01L2300/0636 ,  B01L2300/069 ,  B01L2300/0816 ,  B01L2300/0851 ,  B01L2300/165 ,  B01L2400/0427

摘要： Provided are methods and apparatuses for performing sequencing using droplet manipulation, for example, via electrowetting-based techniques. Also provided are integrated methods and apparatuses for performing sample preparation and sequencing on the same apparatus. In addition, provided are methods of reducing reagent waste and preloaded consumable cartridges comprising reagents for sample preparation and/or sequencing.

摘要翻译： 提供了用于使用液滴操纵进行排序的方法和装置，例如通过基于电润湿的技术。 还提供了用于在同一装置上进行样品制备和测序的综合方法和装置。 此外，提供了减少试剂废物的方法和包括用于样品制备和/或测序的试剂的预装的消耗品筒。

公开(公告)号：US20130116128A1

公开(公告)日：2013-05-09

申请号：US13670318

申请日：2012-11-06

申请人： ILLUMINA, INC.

发明人： Min-Jui Richard Shen ,  Robert C. Kain ,  Kenneth M. Kuhn ,  AmirAli Hajhossein Talasaz ,  Arash Jamshidi ,  George Sakaldasis ,  Eric Vermaas ,  Sebastian Bohm ,  Tarun Khurana ,  Helmy A. Eltoukhy ,  Jian Gong

IPC分类号： C40B60/06 ,  C40B20/00

CPC分类号： C12Q1/6874 ,  B01L3/502792 ,  B01L2200/0673 ,  B01L2300/0636 ,  B01L2300/069 ,  B01L2300/0816 ,  B01L2300/0851 ,  B01L2300/165 ,  B01L2400/0427

摘要： Provided are methods and apparatuses for performing sequencing using droplet manipulation, for example, via electrowetting-based techniques. Also provided are integrated methods and apparatuses for performing sample preparation and sequencing on the same apparatus. In addition, provided are methods of reducing reagent waste and preloaded consumable cartridges comprising reagents for sample preparation and/or sequencing.

摘要翻译： 提供了用于使用液滴操纵进行排序的方法和装置，例如通过基于电润湿的技术。 还提供了用于在同一装置上进行样品制备和测序的综合方法和装置。 此外，提供了减少试剂废物的方法和包括用于样品制备和/或测序的试剂的预装的消耗品筒。

公开(公告)号：US20210262026A1

公开(公告)日：2021-08-26

申请号：US17307973

申请日：2021-05-04

申请人： Illumina, Inc.

发明人： Tatjana Singer ,  Ryan Kelley ,  Gordon Bean ,  Eric Vermaas

IPC分类号： C12Q1/6874 ,  G16B25/00 ,  C12Q1/6869 ,  G16B30/00

摘要： The disclosed embodiments concern index oligonucleotides configured to identify sources of samples of nucleic acids and methods, apparatus, systems and computer program products for identifying and making the index oligonucleotides. In some implementations, the index oligonucleotides include a set of index sequences, a Hamming distance between any two index sequences of the set of index sequences meeting one or more criteria. System, apparatus, and computer program products are also provided for determining a sequence of interest using the index oligonucleotides.

公开(公告)号：US11028436B2

公开(公告)日：2021-06-08

申请号：US15973210

申请日：2018-05-07

申请人： Illumina, Inc.

发明人： Tatjana Singer ,  Ryan Kelley ,  Gordon Bean ,  Eric Vermaas

IPC分类号： C12Q1/6874 ,  G16B25/00 ,  C12Q1/6869 ,  G16B30/00 ,  G16B25/20

摘要： The disclosed embodiments concern index oligonucleotides configured to identify sources of samples of nucleic acids and methods, apparatus, systems and computer program products for identifying and making the index oligonucleotides. In some implementations, the index oligonucleotides include a set of index sequences, a Hamming distance between any two index sequences of the set of index sequences meeting one or more criteria. System, apparatus, and computer program products are also provided for determining a sequence of interest using the index oligonucleotides.