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21 条记录 (耗时 0.028 秒)

    DETECTING ELEMENTS USING SUGAR-LECTIN COUPLINGS
    1.
    发明申请
    DETECTING ELEMENTS USING SUGAR-LECTIN COUPLINGS 有权

    公开(公告)号：US20220082569A1

    公开(公告)日：2022-03-17

    申请号：US17459459

    申请日：2021-08-27

    申请人： ILLUMINA, INC. ,  ILLUMINA SINGAPORE PTE. LTD.

    发明人： Rigo Pantoja ,  Daniel Ortiz ,  Xiangyuan Yang ,  Yin Nah Teo ,  Eric Vermaas ,  Allen Eckhardt

    IPC分类号： G01N33/68 ,  C12Q1/6869

    摘要： A method is provided for detecting an element. The method includes coupling an element-sugar complex to a substrate. The element-sugar complex includes an element coupled to a sugar. The method includes coupling a lectin to the sugar; detecting the lectin; and detecting the element using at least the detection of the lectin. A system is provided that includes a substrate, and an element-sugar complex coupled to the substrate. The element-sugar complex includes an element coupled to a sugar. The system includes a lectin coupled to the sugar; and detection circuitry to detect the element using at least detection of the lectin.

    Conformational probes and methods for sequencing nucleic acids
    3.
    发明授权
    Conformational probes and methods for sequencing nucleic acids 有权

    公开(公告)号：US10233493B2

    公开(公告)日：2019-03-19

    申请号：US15853118

    申请日：2017-12-22

    申请人： Illumina, Inc.

    发明人： Molly He ,  Cheng-Yao Chen ,  Eric Kool ,  Mostafa Ronaghi ,  Michael Previte ,  Rigo Pantoja

    IPC分类号： C12P19/34 ,  C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6818

    摘要： This disclosure provides a method of determining a sequence of nucleotides for a nucleic acid template. The method can include the steps of contacting the nucleic acid template with a conformationally labeled polymerase and at least four different nucleotide species under conditions wherein the conformationally labeled polymerase catalyzes sequential addition of the nucleotide species to form a nucleic acid complement of the nucleic acid template, wherein the sequential addition of each different nucleotide species produces a conformational signal change from the conformationally labeled polymerase and wherein the rate or time duration for the conformational signal change is distinguishable for each different nucleotide species; detecting a series of changes in the signal from the conformationally labeled polymerase under the conditions; and determining the rates or time durations for the changes in the signal, thereby determining the sequence of nucleotides for the nucleic acid template.

    APPARATUS AND METHODS FOR KINETIC ANALYSIS AND DETERMINATION OF NUCLEIC ACID SEQUENCES
    5.
    发明申请
    APPARATUS AND METHODS FOR KINETIC ANALYSIS AND DETERMINATION OF NUCLEIC ACID SEQUENCES 审中-公开

    公开(公告)号：US20190002973A1

    公开(公告)日：2019-01-03

    申请号：US16127067

    申请日：2018-09-10

    申请人： Illumina, Inc.

    发明人： Michael Previte ,  Molly He ,  Rigo Pantoja ,  Cheng-Yao Chen ,  Chunhong Zhou

    IPC分类号： C12Q1/6874 ,  C12Q1/6869

    摘要： A method of distinguishing nucleotide sequences for different nucleic acid molecules including the steps of (a) mixing a plurality of different nucleic acid molecules with polymerase molecules and nucleotide molecules, wherein the different nucleic acid molecules are attached to a surface in the form of an array of nucleic acid features; (b) determining a transient state of the polymerase molecules at the nucleic acid features; and (c) identifying a subset of nucleic acid features that correctly incorporate the nucleotide molecules based on the transient state of the polymerase molecules at the nucleic acid features, thereby distinguishing the nucleotide sequences for the different nucleic acid molecules.

    Conformational probes and methods for sequencing nucleic acids
    6.
    发明授权
    Conformational probes and methods for sequencing nucleic acids 有权

    公开(公告)号：US09862998B2

    公开(公告)日：2018-01-09

    申请号：US15132662

    申请日：2016-04-19

    申请人： Illumina, Inc.

    发明人： Molly He ,  Cheng-Yao Chen ,  Eric Kool ,  Mostafa Ronaghi ,  Michael Previte ,  Rigo Pantoja

    IPC分类号： C12P19/34 ,  C12Q1/68

    CPC分类号： C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6818 ,  C12Q2565/133 ,  C12Q2563/125 ,  C12Q2525/101 ,  C12Q2565/1015

    摘要： This disclosure provides a method of determining a sequence of nucleotides for a nucleic acid template. The method can include the steps of contacting the nucleic acid template with a conformationally labeled polymerase and at least four different nucleotide species under conditions wherein the conformationally labeled polymerase catalyzes sequential addition of the nucleotide species to form a nucleic acid complement of the nucleic acid template, wherein the sequential addition of each different nucleotide species produces a conformational signal change from the conformationally labeled polymerase and wherein the rate or time duration for the conformational signal change is distinguishable for each different nucleotide species; detecting a series of changes in the signal from the conformationally labeled polymerase under the conditions; and determining the rates or time durations for the changes in the signal, thereby determining the sequence of nucleotides for the nucleic acid template.

    APPARATUS AND METHODS FOR KINETIC ANALYSIS AND DETERMINATION OF NUCLEIC ACID SEQUENCES
    7.
    发明申请
    APPARATUS AND METHODS FOR KINETIC ANALYSIS AND DETERMINATION OF NUCLEIC ACID SEQUENCES 有权
    用于动力学分析和核酸序列测定的装置和方法

    公开(公告)号：US20130165328A1

    公开(公告)日：2013-06-27

    申请号：US13722979

    申请日：2012-12-20

    申请人： Illumina, Inc.

    发明人： Michael Previte ,  Molly He ,  Rigo Pantoja ,  Cheng-Yao Chen ,  Chunhong Zhou

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6874 ,  C12Q1/6869 ,  C12Q2537/149 ,  C12Q2561/12 ,  C12Q2565/518 ,  C12Q2565/537

    摘要： A method of distinguishing nucleotide sequences for different nucleic acid molecules including the steps of (a) mixing a plurality of different nucleic acid molecules with polymerase molecules and nucleotide molecules, wherein the different nucleic acid molecules are attached to a surface in the form of an array of nucleic acid features; (b) determining a transient state of the polymerase molecules at the nucleic acid features; and (c) identifying a subset of nucleic acid features that correctly incorporate the nucleotide molecules based on the transient state of the polymerase molecules at the nucleic acid features, thereby distinguishing the nucleotide sequences for the different nucleic acid molecules.

    摘要翻译： 区分不同核酸分子的核苷酸序列的方法，包括以下步骤：（a）将多种不同的核酸分子与聚合酶分子和核苷酸分子混合，其中不同的核酸分子以阵列的形式附着在表面上 的核酸特征; （b）确定聚合酶分子在核酸特征处的瞬时状态; 和（c）鉴定核酸特征的子集，其核酸分子基于聚合酶分子在核酸特征处的瞬时状态正确地掺入，从而区分不同核酸分子的核苷酸序列。

    Apparatus and methods for kinetic analysis and determination of nucleic acid sequences
    9.
    发明授权
    Apparatus and methods for kinetic analysis and determination of nucleic acid sequences 有权

    公开(公告)号：US11242560B2

    公开(公告)日：2022-02-08

    申请号：US16127067

    申请日：2018-09-10

    申请人： Illumina, Inc.

    发明人： Michael Previte ,  Molly He ,  Rigo Pantoja ,  Cheng-Yao Chen ,  Chunhong Zhou

    IPC分类号： C12Q1/6874

    摘要： A method of distinguishing nucleotide sequences for different nucleic acid molecules including the steps of (a) mixing a plurality of different nucleic acid molecules with polymerase molecules and nucleotide molecules, wherein the different nucleic acid molecules are attached to a surface in the form of an array of nucleic acid features; (b) determining a transient state of the polymerase molecules at the nucleic acid features; and (c) identifying a subset of nucleic acid features that correctly incorporate the nucleotide molecules based on the transient state of the polymerase molecules at the nucleic acid features, thereby distinguishing the nucleotide sequences for the different nucleic acid molecules.

    NUCLEIC ACID CAPTURE, CONCENTRATION, AND PURIFICATION
    10.
    发明申请
    NUCLEIC ACID CAPTURE, CONCENTRATION, AND PURIFICATION 有权

    公开(公告)号：US20220001384A1

    公开(公告)日：2022-01-06

    申请号：US17358783

    申请日：2021-06-25

    申请人： ILLUMINA, INC.

    发明人： Sean M. Ramirez ,  Anmiv Prabhu ,  Rigo Pantoja ,  Michelle Higgins

    IPC分类号： B01L3/00 ,  B01L7/00 ,  G01N35/10

    摘要： An example of a kit includes a flow cell assembly. The flow cell assembly includes a reaction chamber, a temperature controlled flow channel in selective fluid communication with an inlet of the reaction chamber, and a filter positioned in the temperature controlled flow channel. The reaction chamber includes depressions separated by interstitial regions and capture primers attached within each of the depressions. The filter is i) to block concentrated biological sample-polymer complexes generated in the temperature controlled flow channel at a first temperature, and ii) to allow passage of concentrated biological sample and polymer released from the complexes in the temperature controlled flow channel at a second temperature.