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公开(公告)号：US09498763B2
公开(公告)日：2016-11-22
申请号：US14738591
申请日：2015-06-12
发明人： Xiaohai Liu , John Milton , Geoffrey Paul Smith , Colin Lloyd Barnes , Isabelle Rasolonjatovo , Roberto Rigatti , Xiaolin Wu , Tobias William Barr Ost , Graham John Worsley , David James Earnshaw , Gerardo Turcatti , Anthony Romieu
CPC分类号： B01J19/0046 , B01J2219/00596 , B01J2219/00608 , B01J2219/00675 , B01J2219/00722 , C12Q1/6806 , C12Q1/6834 , C12Q1/6874 , C12Q2565/543 , C12Q2535/101 , C12Q2523/113 , C12Q2523/107 , C12Q2521/331 , C12Q2521/307
摘要： The invention relates to methods of generating templates for a nucleic acid sequencing reaction which comprise: providing at least one double-stranded nucleic acid molecule, wherein both strands of the double-stranded nucleic acid molecule are attached to a solid support at the 5′ end, cleaving one or both strands of the double-stranded nucleic acid molecule, and subjecting the cleaved strand(s) to denaturing conditions to remove the portion of the cleaved strand(s) not attached to the solid support, thereby generating a partially or substantially single-stranded template for a nucleic acid sequencing reaction.
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公开(公告)号：US10710046B2
公开(公告)日：2020-07-14
申请号：US16010902
申请日：2018-06-18
发明人： Xiaohai Liu , John Milton , Geoffrey Paul Smith , Colin Lloyd Barnes , Isabelle Rasolonjatovo , Roberto Rigatti , Xiaolin Wu , Tobias William Barr Ost , Graham John Worsley , David James Earnshaw , Gerardo Turcatti , Anthony Romieu
IPC分类号： C12Q1/6806 , C12Q1/6874 , B01J19/00 , C12Q1/6834
摘要： The invention relates to methods of generating templates for a nucleic acid sequencing reaction which comprise: providing at least one double-stranded nucleic acid molecule, wherein both strands of the double-stranded nucleic acid molecule are attached to a solid support at the 5′ end, cleaving one or both strands of the double-stranded nucleic acid molecule, and subjecting the cleaved strand(s) to denaturing conditions to remove the portion of the cleaved strand(s) not attached to the solid support, thereby generating a partially or substantially single-stranded template for a nucleic acid sequencing reaction.
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公开(公告)号：US20180363047A1
公开(公告)日：2018-12-20
申请号：US16004714
申请日：2018-06-11
发明人： Geoffrey Paul Smith , Jonathan Mark Boutell , Colin Lloyd Barnes , Roberto Rigatti , Niall Anthony Gormley , David Bentley , Tobias William Barr Ost , Vincent Peter Smith , Graham John Worsley , Eric Hans Vermaas
IPC分类号： C12Q1/6874 , C12Q1/6869
摘要： The invention provides methods for pairwise sequencing of a double-stranded polynucleotide template, which methods result in the sequential determination of nucleotide sequences in two distinct and separate regions of the polynucleotide template.
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公开(公告)号：US10519496B2
公开(公告)日：2019-12-31
申请号：US15470801
申请日：2017-03-27
IPC分类号： C12Q1/68 , C07H19/10 , C12Q1/6869 , C07H19/20 , C07H21/00 , C12P19/34 , C12Q1/6876 , C12Q1/6874 , C12Q1/6818 , C12Q1/6834 , C12Q1/6806 , C12Q1/6823
摘要： Nucleosides and nucleotides are disclosed that are linked to detectable labels via a cleavable linker group.
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公开(公告)号：US20150203910A1
公开(公告)日：2015-07-23
申请号：US14574023
申请日：2014-12-17
发明人： Geoffrey Paul Smith , Jonathan Mark Boutell , Colin Lloyd Barnes , Roberto Rigatti , Niall Anthony Gormley , David Bentley , Tobias William Barr Ost , Vincent Peter Smith , Graham John Worsley , Eric Hans Vermaas
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , C12Q1/6869 , C12Q2565/518 , C12Q2537/149
摘要： The invention provides methods for pairwise sequencing of a double-stranded polynucleotide template, which methods result in the sequential determination of nucleotide sequences in two distinct and separate regions of the polynucleotide template.
摘要翻译： 本发明提供了双链多核苷酸模板的成对测序的方法，该方法导致多核苷酸模板的两个不同和分离的区域中的核苷酸序列的顺序测定。
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公开(公告)号：US10480025B2
公开(公告)日：2019-11-19
申请号：US15202456
申请日：2016-07-05
发明人： Shankar Balasubramanian , Colin Lloyd Barnes , Xiaohai Liu , John Milton , Xiaolin Wu , Harold Swerdlow
IPC分类号： C07H21/00 , C12Q1/6869 , C07H19/10 , C07H19/20 , C12P19/34 , C12Q1/6876 , C12Q1/6874 , C12Q1/6818 , C12Q1/6834 , C12Q1/6806 , C12Q1/6823 , C12Q1/68
摘要： Nucleosides and nucleotides are disclosed that are linked to detectable labels via a cleavable linker group.
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公开(公告)号：US20180353926A1
公开(公告)日：2018-12-13
申请号：US16010902
申请日：2018-06-18
发明人： Xiaohai Liu , John Milton , Geoffrey Paul Smith , Colin Lloyd Barnes , Isabelle Rasolonjatovo , Roberto Rigatti , Xiaolin Wu , Tobias William Barr Ost , Graham John Worsley , David James Earnshaw , Gerardo Turcatti , Anthony Romieu
IPC分类号： B01J19/00 , C12Q1/6806 , C12Q1/6834 , C12Q1/6874
CPC分类号： B01J19/0046 , B01J2219/00596 , B01J2219/00608 , B01J2219/00675 , B01J2219/00722 , C12Q1/6806 , C12Q1/6834 , C12Q1/6874 , C12Q2565/543 , C12Q2535/101 , C12Q2523/113 , C12Q2523/107 , C12Q2521/331 , C12Q2521/307
摘要： The invention relates to methods of generating templates for a nucleic acid sequencing reaction which comprise: providing at least one double-stranded nucleic acid molecule, wherein both strands of the double-stranded nucleic acid molecule are attached to a solid support at the 5′ end, cleaving one or both strands of the double-stranded nucleic acid molecule, and subjecting the cleaved strand(s) to denaturing conditions to remove the portion of the cleaved strand(s) not attached to the solid support, thereby generating a partially or substantially single-stranded template for a nucleic acid sequencing reaction.
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公开(公告)号：US09999866B2
公开(公告)日：2018-06-19
申请号：US15287077
申请日：2016-10-06
发明人： Xiaohai Liu , John Milton , Geoffrey Paul Smith , Colin Lloyd Barnes , Isabelle Rasolonjatovo , Roberto Rigatti , Xiaolin Wu , Tobias William Barr Ost , Graham John Worsley , David James Earnshaw , Gerardo Turcatti , Anthony Romieu
CPC分类号： B01J19/0046 , B01J2219/00596 , B01J2219/00608 , B01J2219/00675 , B01J2219/00722 , C12Q1/6806 , C12Q1/6834 , C12Q1/6874 , C12Q2565/543 , C12Q2535/101 , C12Q2523/113 , C12Q2523/107 , C12Q2521/331 , C12Q2521/307
摘要： The invention relates to methods of generating templates for a nucleic acid sequencing reaction which comprise: providing at least one double-stranded nucleic acid molecule, wherein both strands of the double-stranded nucleic acid molecule are attached to a solid support at the 5′ end, cleaving one or both strands of the double-stranded nucleic acid molecule, and subjecting the cleaved strand(s) to denaturing conditions to remove the portion of the cleaved strand(s) not attached to the solid support, thereby generating a partially or substantially single-stranded template for a nucleic acid sequencing reaction.
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公开(公告)号：US09994896B2
公开(公告)日：2018-06-12
申请号：US14574023
申请日：2014-12-17
发明人： Geoffrey Paul Smith , Jonathan Mark Boutell , Colin Lloyd Barnes , Roberto Rigatti , Niall Anthony Gormley , David Bentley , Tobias William Barr Ost , Vincent Peter Smith , Graham John Worsley , Eric Hans Vermaas
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , C12Q1/6869 , C12Q2565/518 , C12Q2537/149
摘要： The invention provides methods for pairwise sequencing of a double-stranded polynucleotide template, which methods result in the sequential determination of nucleotide sequences in two distinct and separate regions of the polynucleotide template.
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公开(公告)号：US20170021325A1
公开(公告)日：2017-01-26
申请号：US15287077
申请日：2016-10-06
发明人： Xiaohai Liu , John Milton , Geoffrey Paul Smith , Colin Lloyd Barnes , Isabelle Rasolonjatovo , Roberto Rigatti , Xiaolin Wu , Tobias William Barr Ost , Graham John Worsley , David James Earnshaw , Gerardo Turcatti , Anthony Romieu
CPC分类号： B01J19/0046 , B01J2219/00596 , B01J2219/00608 , B01J2219/00675 , B01J2219/00722 , C12Q1/6806 , C12Q1/6834 , C12Q1/6874 , C12Q2565/543 , C12Q2535/101 , C12Q2523/113 , C12Q2523/107 , C12Q2521/331 , C12Q2521/307
摘要： The invention relates to methods of generating templates for a nucleic acid sequencing reaction which comprise: providing at least one double-stranded nucleic acid molecule, wherein both strands of the double-stranded nucleic acid molecule are attached to a solid support at the 5′ end, cleaving one or both strands of the double-stranded nucleic acid molecule, and subjecting the cleaved strand(s) to denaturing conditions to remove the portion of the cleaved strand(s) not attached to the solid support, thereby generating a partially or substantially single-stranded template for a nucleic acid sequencing reaction.
摘要翻译： 本发明涉及产生用于核酸测序反应的模板的方法，其包括：提供至少一个双链核酸分子，其中双链核酸分子的两条链在5'末端连接到固体支持物上 切割双链核酸分子的一条或两条链，并使切割的链进行变性条件以除去未连接到固体支持物上的部分切割的链，从而产生部分或基本上 用于核酸测序反应的单链模板。
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