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    Multilayer membrane useful as synthetic skin
    2.
    发明授权
    Multilayer membrane useful as synthetic skin 失效
    多层膜可用作合成皮肤

    公开(公告)号：US4060081A

    公开(公告)日：1977-11-29

    申请号：US596112

    申请日：1975-07-15

    申请人： Ioannis V. Yannas ,  John F. Burke ,  Philip L. Gordon ,  Chor Huang

    发明人： Ioannis V. Yannas ,  John F. Burke ,  Philip L. Gordon ,  Chor Huang

    IPC分类号： B32B9/00 ,  A61F2/00 ,  A61F2/02 ,  A61F2/10 ,  A61L15/22 ,  A61L27/00 ,  A61L27/24 ,  A61L33/10 ,  B01D61/00 ,  B01D71/08 ,  A61L15/00

    CPC分类号： A61L27/24 ,  A61F2/105 ,  A61L15/225 ,  A61L27/60 ,  A61F2310/00365 ,  Y10S128/08

    摘要： A multilayer membrane, which is useful as synthetic skin, is disclosed herein. A first layer is formed from a material which does not provoke an immune response and which is also insoluble and nondegradable in the presence of body fluids and/or body enzymes. Preferred materials for the first layer are crosslinked composites of collagen and a mucopolysaccharide. A second layer is formed from a nontoxic material which controls the moisture flux of the overall membrane to about 0.1 to 1 mg./cm.sup.2 /hr. Suitable materials for the second layer include synthetic polymers such as silicone resins, polyacrylate or polymethacrylate esters or their copolymers, and polyurethanes.

    摘要翻译： 本文公开了可用作合成皮肤的多层膜。 第一层由不引起免疫应答的材料形成，并且在体液和/或体内酶的存在下也是不溶的和不可降解的。 用于第一层的优选材料是胶原和粘多糖的交联复合物。 第二层由无毒材料形成，其将整个膜的水分通量控制在约0.1至1mg./cm 2 / hr。 用于第二层的合适的材料包括合成聚合物，例如硅树脂，聚丙烯酸酯或聚甲基丙烯酸酯或它们的共聚物，以及聚氨酯。

    Cell-seeding procedures involving fibrous lattices
    4.
    发明授权
    Cell-seeding procedures involving fibrous lattices 失效
    涉及纤维网格的细胞播种程序

    公开(公告)号：US4458678A

    公开(公告)日：1984-07-10

    申请号：US315233

    申请日：1981-10-26

    申请人： Ioannis V. Yannas ,  John F. Burke

    发明人： Ioannis V. Yannas ,  John F. Burke

    IPC分类号： A61K35/36 ,  A61F2/00 ,  A61K31/715 ,  A61K38/00 ,  A61L15/00 ,  A61L15/64 ,  A61L27/24 ,  A61L27/60 ,  A61P17/00 ,  A61F13/00

    CPC分类号： A61L27/60 ,  A61L27/24 ,  A61F2310/00365

    摘要： This invention relates to the introduction of viable cells into a fibrous lattice by surgical, force-utilizing, or other manipulative techniques, all of which are referred to herein as "seeding." One embodiment comprises an autografting technique which involves intact tissue. Other embodiments which involve the distribution of an aqueous suspension of cells comprise centrifugal, spraying, pipetting and syringe emplacement. Each cell that survives the seeding process may reproduce into a colony of cells which grow until they reach confluence, thereby creating a uniform layer or volume of tissue. By proper placement of cells capable of generating such colonies, the period of recovery of a wound may be drastically reduced. In addition, research by the Applicants indicates that tissue that is regenerated using the methods of this invention to seed a lattice comprising collagen and glycosaminoglycan tends to be smoother, more uniform, less fibrotic, less scarred and more flexible than tissue that is regenerated by wound contraction or conventional autografting.

    摘要翻译： 本发明涉及通过手术，强力利用或其他操作技术将活细胞引入纤维网格，所有这些在本文中称为“播种”。 一个实施例包括涉及完整组织的自动移植技术。 涉及细胞水性悬浮液分布的其它实施方案包括离心，喷雾，移液和注射器放置。 在接种过程中存活的每个细胞可以再生成细胞的集落，其生长直到它们达到汇合，从而产生均匀的组织或体积的组织。 通过适当放置能够产生这种菌落的细胞，伤口的恢复时间可以大大降低。 此外，申请人的研究表明，使用本发明的方法再生的组织使含有胶原蛋白和糖胺聚糖的晶格种子的组织趋于比由伤口再生的组织更平滑，更均匀，更少的纤维化，更少的伤痕和更灵活 收缩或常规自动移植。

    Method of using a fibrous lattice
    5.
    发明授权
    Method of using a fibrous lattice 失效
    使用纤维网格的方法

    公开(公告)号：US4505266A

    公开(公告)日：1985-03-19

    申请号：US601233

    申请日：1984-04-17

    申请人： Ioannis V. Yannas ,  John F. Burke

    发明人： Ioannis V. Yannas ,  John F. Burke

    IPC分类号： A61F2/00 ,  A61L27/24 ,  A61L27/60 ,  A61B19/00

    CPC分类号： A61L27/24 ,  A61L27/60 ,  A61F2310/00365 ,  Y10S623/915

    摘要： This invention relates to the introduction of viable cells into a fibrous lattice by surgical, force-utilizing, or other manipulative techniques, all of which are referred to herein as "seeding." One embodiment comprises an autografting technique which involves intact tissue. Other embodiments which involve the distribution of an aqueous suspension of cells comprise centrifugal, spraying, pipetting and syringe emplacement. Each cell that survives the seeding process may reproduce into a colony of cells which grow until they reach confluence, thereby creating a uniform layer or volume of tissue. By proper placement of cells capable of generating such colonies, the period of recovery of a wound may be drastically reduced. In addition, research by the Applicants indicates that tissue that is regenerated using the methods of this invention to seed a lattice comprising collagen and glycosaminoglycan tends to be smoother, more uniform, less fibrotic, less scarred and more flexible than tissue that is regenerated by wound contraction or conventional autografting.

    摘要翻译： 本发明涉及通过手术，强力利用或其他操作技术将活细胞引入纤维网格，所有这些在本文中称为“播种”。 一个实施例包括涉及完整组织的自动移植技术。 涉及细胞水性悬浮液分布的其它实施方案包括离心，喷雾，移液和注射器放置。 在接种过程中存活的每个细胞可以再生成细胞的集落，其生长直到它们达到汇合，从而产生均匀的组织或体积的组织。 通过适当放置能够产生这种菌落的细胞，伤口的恢复时间可以大大降低。 此外，申请人的研究表明，使用本发明的方法再生的组织使含有胶原蛋白和糖胺聚糖的晶格种子的组织趋于比由伤口再生的组织更平滑，更均匀，更少的纤维化，更少的伤痕和更灵活 收缩或常规自动移植。

    Method of promoting the regeneration of tissue at a wound
    6.
    发明授权
    Method of promoting the regeneration of tissue at a wound 失效
    促进伤口组织再生的方法

    公开(公告)号：US4418691A

    公开(公告)日：1983-12-06

    申请号：US315234

    申请日：1981-10-26

    申请人： Ioannis V. Yannas ,  John F. Burke ,  Dennis P. Orgill ,  Eugene M. Skrabut

    发明人： Ioannis V. Yannas ,  John F. Burke ,  Dennis P. Orgill ,  Eugene M. Skrabut

    IPC分类号： A61K35/36 ,  A61K31/715 ,  A61K38/00 ,  A61L15/00 ,  A61L15/64 ,  A61L26/00 ,  A61L27/60 ,  A61P17/00

    CPC分类号： A61L27/60 ,  Y10S623/915

    摘要： This invention comprises the use of centrifugal force to introduce viable cells into a fibrous lattice, as well as fibrous lattices that are seeded with cells by the use of centrifugal force. A variety of fibrous lattices may be seeded by the methods of this invention, such as a highly porous lattice comprising collagen fibers crosslinked with glycosaminoglycan. Before the centrifugation, a piece of intact tissue is harvested from a donor site. It is treated with one or more substances, such as trypsin or collagenase, to dissociate cells from the tissue. The cells are then mixed with an aqueous solution to create an aqueous suspension of cells. A piece of fibrous lattice is placed within a container, referred to herein as a "bucket," that is suitable for rotation by a centrifuge. The aqueous suspension of cells is placed within the bucket, in contact with the lattice. The centrifuge is then rotated. Centrifugal force causes the cells, which are denser than the aqueous solution, to be forced into the lattice in a relatively uniform distribution. By controlling various parameters, cells can be seeded into any desired location within a lattice.

    摘要翻译： 本发明包括使用离心力将活细胞引入纤维网格，以及通过使用离心力与细胞接种的纤维网格。 各种纤维网格可以通过本发明的方法接种，例如包含与糖胺聚糖交联的胶原纤维的高度多孔的网格。 在离心之前，从供体部位收获一块完整的组织。 用一种或多种物质（例如胰蛋白酶或胶原酶）处理以从组织中分离细胞。 然后将细胞与水溶液混合以产生细胞的水悬浮液。 将一片纤维格子放置在适合于离心机旋转的容器内，这里称为“桶”。 将细胞的水悬浮液置于桶内，与晶格接触。 然后旋转离心机。 离心力使得比水溶液更致密的细胞以相对均匀的分布被迫进入晶格。 通过控制各种参数，可将细胞接种到晶格内的任何所需位置。

    Biomaterial
    7.
    发明授权
    Biomaterial 有权
    生物材料

    公开(公告)号：US08318902B2

    公开(公告)日：2012-11-27

    申请号：US12377221

    申请日：2007-08-10

    申请人： Andrew Lynn ,  William Bonfield ,  Zachary D. Wissner-Gross ,  Brendan A. Harley ,  Ioannis V. Yannas ,  Lorna J. Gibson

    发明人： Andrew Lynn ,  William Bonfield ,  Zachary D. Wissner-Gross ,  Brendan A. Harley ,  Ioannis V. Yannas ,  Lorna J. Gibson

    IPC分类号： A61K38/17

    CPC分类号： A61L27/46 ,  A61L27/48 ,  A61L27/56 ,  C08L89/06

    摘要： A process for the preparation of a composite biomaterial comprising: providing a first substantially solid component comprising one or more of collagen, a glycosaminoglycan, albumin, hyaluronan, chitosan, and synthetic polypeptides comprising a portion of the polypeptide sequence of collagen, and optionally an inorganic material, said component having at least a surface portion that is porous; providing a fluid composition comprising one or more of collagen, a glycosaminoglycan, albumin, hyaluronan, chitosan, and synthetic polypeptides comprising a portion of the polypeptide sequence of collagen, and a liquid carrier, and optionally an inorganic material; contacting said fluid composition with said porous surface portion of said first component; cooling said fluid composition to a temperature at which the liquid carrier transforms into a plurality of solid crystals or particles; removing at least some of the plurality of solid crystals or particles by sublimation and/or evaporation.

    摘要翻译： 一种制备复合生物材料的方法，包括：提供包含胶原，糖胺聚糖，白蛋白，透明质酸，壳聚糖和包含胶原多肽序列的一部分的合成多肽中的一种或多种的第一基本上固体的组分，以及任选的无机 所述组分至少具有多孔的表面部分; 提供包含胶原，糖胺聚糖，白蛋白，透明质酸，壳聚糖和包含胶原多肽序列的一部分的合成多肽，液体载体和任选的无机材料中的一种或多种的流体组合物; 使所述流体组合物与所述第一组分的所述多孔表面部分接触; 将所述流体组合物冷却至液体载体转变成多个固体晶体或颗粒的温度; 通过升华和/或蒸发去除多个固体晶体或颗粒中的至少一些。

    Cross flow filtration molding method
    9.
    发明授权
    Cross flow filtration molding method 失效
    交叉流过滤成型法

    公开(公告)号：US4252759A

    公开(公告)日：1981-02-24

    申请号：US029229

    申请日：1979-04-11

    申请人： Ioannis V. Yannas ,  David L. Sieverding

    发明人： Ioannis V. Yannas ,  David L. Sieverding

    IPC分类号： A61F2/00 ,  A61F2/06 ,  A61L27/24 ,  B28B1/26 ,  B29C41/16

    CPC分类号： B28B1/26 ,  A61F2/062 ,  A61L27/24 ,  B29C41/16 ,  A61F2310/00365

    摘要： A cross flow filtration molding apparatus and method are disclosed which are particularly useful for forming complicated shapes from dispersions of particles in a liquid medium. Dispersion is pumped through a mold which has porous walls and a sufficient pressure differential is applied to drive a portion of the liquid medium through the porous walls which results in deposition of particles on the walls to form a shaped article. The shaped article may inherently have sufficient structural integrity, or it may be post-treated to provide additional structural integrity.

    摘要翻译： 公开了一种交叉流过滤成型装置和方法，其特别可用于从液体介质中的颗粒分散形成复杂形状。 将分散体泵送通过具有多孔壁的模具，并且施加足够的压差以通过多孔壁驱动一部分液体介质，导致颗粒沉积在壁上以形成成型制品。 成型制品本来可以具有足够的结构完整性，或者可以进行后处理以提供额外的结构完整性。