公开(公告)号：US11162129B2

公开(公告)日：2021-11-02

申请号：US15799139

申请日：2017-10-31

申请人： IsoPlexis Corporation

发明人： Luisa Andruzzi ,  Michel Georges Perbost ,  Dona Hevroni ,  Minakshi Guha ,  Austin Ricker ,  Timothy Pelletier

IPC分类号： C12Q1/68 ,  C12Q1/6806 ,  C12Q1/6816 ,  C07C215/28 ,  C07D311/66 ,  C12Q1/6874 ,  G01N33/58

摘要： The invention relates to methods, compositions, devices, systems and kits as described including, without limitation, reagents and mixtures for determining the identity of nucleic acids in nucleotide sequences using, for example, sequencing by synthesis methods. In particular, the present invention contemplates the use of photoprotective mixture of compounds as imaging reagents to improve stability and storage of fluorescent compounds, including but not limited to, nucleotides with fluorescent labels.

公开(公告)号：US11821032B2

公开(公告)日：2023-11-21

申请号：US16999329

申请日：2020-08-21

申请人： IsoPlexis Corporation

发明人： Luisa Andruzzi ,  Jimmy Adediran ,  Timothy Pelletier ,  Austin Ricker ,  Angela DeLucia ,  Ben McNally ,  Dona Hevroni ,  Mong Sano Marma ,  John Andrew Sheridan

IPC分类号： C12P19/34 ,  C12Q1/6869 ,  C12Q1/6876 ,  C12Q1/6806 ,  C07D275/03

CPC分类号： C12Q1/6869 ,  C12Q1/6806 ,  C12Q1/6876 ,  C07D275/03 ,  C12Q2525/113 ,  C12Q2525/186 ,  C12Q2600/16 ,  C12Q1/6869 ,  C12Q2525/113 ,  C12Q2525/117 ,  C12Q2525/186

摘要： The present invention provides methods, compositions, mixtures and kits utilizing 5-Chloro-2-methyl-4-isothiazolin-3-one in sequencing reactions, and in particular, sequencing reactions where deoxynucleoside triphosphates comprising a 3′-O position capped by a disulfide-based 3′-terminator group are used. In one embodiment, the deoxynucleoside triphosphates comprise a 3′-O position capped by a group comprising methylenedisulfide as a cleavable protecting group and a detectable label reversibly connected to the nucleobase of said deoxynucleoside. In addition, thiol-containing compounds and scavengers of thio-containing compounds are described. Such compounds provide new possibilities for future sequencing technologies, including but not limited to Sequencing by Synthesis.

公开(公告)号：US11421264B2

公开(公告)日：2022-08-23

申请号：US15457344

申请日：2017-03-13

申请人： IsoPlexis Corporation

发明人： Mong Sano Marma ,  Luisa Andruzzi ,  Ben McNally ,  Angela DeLucia ,  Dona Hevroni ,  Elizabeth Carpenter ,  Magnus Besev

IPC分类号： C12Q1/68 ,  C12Q1/6823 ,  C07D405/14 ,  C07F9/6561 ,  C07D405/04 ,  C07D491/22 ,  C12Q1/6874 ,  C07H19/14 ,  C07H19/10 ,  C07D519/00 ,  C07F9/6558 ,  C07F7/18 ,  C09B23/08 ,  C09B11/24

摘要： The present invention provides methods, compositions, mixtures and kits utilizing deoxynucleoside triphosphates comprising a 3′-O position capped by a group comprising methylenedisulfide as a cleavable protecting group and a detectable label reversibly connected to the nucleobase of said deoxynucleoside. In addition, thiol-containing compounds and scavengers of thio-containing compounds are described. Such compounds provide new possibilities for future sequencing technologies, including but not limited to Sequencing by Synthesis.