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    Method for production of TRACP5b
    3.
    发明授权
    Method for production of TRACP5b 有权
    生产TRACP5b的方法

    公开(公告)号：US08426674B2

    公开(公告)日：2013-04-23

    申请号：US12521701

    申请日：2007-12-28

    申请人： Iwao Kiyokawa ,  Tatsuya Ohashi ,  Toshihide Miura ,  Katsuhiro Katayama ,  Toshiki Tamura ,  Isao Kobayashi ,  Hideki Sezutsu

    发明人： Iwao Kiyokawa ,  Tatsuya Ohashi ,  Toshihide Miura ,  Katsuhiro Katayama ,  Toshiki Tamura ,  Isao Kobayashi ,  Hideki Sezutsu

    IPC分类号： A01K67/04

    CPC分类号： C12N9/16

    摘要： Silkworms which have (i) a DNA encoding a transcriptional regulator operably linked downstream of a promoter of a DNA encoding a protein specifically expressed in the silk gland and (ii) a DNA encoding TRACP5 operably linked downstream of a target promoter of the transcriptional regulator were produced. The result showed that active TRACP5b was produced from the silkworms. This means that TRACP5 produced from the silk gland of the silkworms undergoes processing in the silk gland that is similar to the processing taking place at bone resorption sites.

    摘要翻译： 蚕具有（i）编码转录调节子的DNA，其可操作地连接在编码丝腺特异性表达的蛋白质的DNA的启动子的下游，和（ii）可操作地连接在转录调节子的靶启动子下游的编码TRACP5的DNA） 生产。 结果表明，活性TRACP5b是从蚕生产的。 这意味着从蚕丝腺产生的TRACP5在丝腺中进行加工，类似于在骨吸收部位发生的加工。

    METHOD FOR PRODUCTION OF TRACP5b
    4.
    发明申请
    METHOD FOR PRODUCTION OF TRACP5b 有权
    生产TRACP5b的方法

    公开(公告)号：US20110239313A1

    公开(公告)日：2011-09-29

    申请号：US12521701

    申请日：2007-12-28

    申请人： Iwao Kiyokawa ,  Tatsuya Ohashi ,  Toshihide Miura ,  Katsuhiro Katayama ,  Toshiki Tamura ,  Isao Kobayashi ,  Hideki Sezutsu

    发明人： Iwao Kiyokawa ,  Tatsuya Ohashi ,  Toshihide Miura ,  Katsuhiro Katayama ,  Toshiki Tamura ,  Isao Kobayashi ,  Hideki Sezutsu

    IPC分类号： C12P21/00 ,  C12N15/85 ,  A01K67/04

    CPC分类号： C12N9/16

    摘要： Silkworms which have (i) a DNA encoding a transcriptional regulator operably linked downstream of a promoter of a DNA encoding a protein specifically expressed in the silk gland and (ii) a DNA encoding TRACP5 operably linked downstream of a target promoter of the transcriptional regulator were produced. The result showed that active TRACP5b was produced from the silkworms. This means that TRACP5 produced from the silk gland of the silkworms undergoes processing in the silk gland that is similar to the processing taking place at bone resorption sites.

    摘要翻译： 蚕具有（i）编码转录调节子的DNA，其可操作地连接在编码丝腺特异性表达的蛋白质的DNA的启动子的下游，和（ii）可操作地连接在转录调节子的靶启动子下游的编码TRACP5的DNA） 生产。 结果表明，活性TRACP5b由蚕生产。 这意味着从蚕丝腺产生的TRACP5在丝腺中进行加工，类似于在骨吸收部位发生的加工。

    Methods for producing proteins using silkworm middle silk gland-specific gene expression system
    5.
    发明申请
    Methods for producing proteins using silkworm middle silk gland-specific gene expression system 有权
    使用蚕丝绸腺特异性基因表达系统生产蛋白质的方法

    公开(公告)号：US20060070132A1

    公开(公告)日：2006-03-30

    申请号：US11085838

    申请日：2005-03-21

    申请人： Toshiki Tamura ,  Hideki Sezutsu ,  Isao Kobayashi ,  Katsura Kojima ,  Toshio Kanda ,  Keiro Uchino

    发明人： Toshiki Tamura ,  Hideki Sezutsu ,  Isao Kobayashi ,  Katsura Kojima ,  Toshio Kanda ,  Keiro Uchino

    IPC分类号： A01K67/033

    CPC分类号： C12N15/8509 ,  A01K67/0333 ,  A01K67/04 ,  A01K2217/05 ,  A01K2227/706 ,  A01K2267/01 ,  A01K2267/0393

    摘要： Transgenic silkworms comprising GFP whose expression is regulated by the sericin gene promoter were produced. Observation of the silk glands of the last instar larvae of the silkworms showed fluorescence only in the middle silk glands. GFP was secreted from middle silkgland cells from around the spinning stage, indicating that GFP moved into the gland lumen. Finally, GFP was spun into cocoon filaments, and cocoons containing large amounts of GFP were produced. Thus, by using the promoter region of the sericin gene, recombinant proteins can be produced in the middle silk glands. Furthermore, the recombinant proteins produced in the middle silk glands were readily secreted into the lumen of the middle silk glands.

    摘要翻译： 产生包含GFP的转基因蚕，其表达受丝胶蛋白基因启动子调节。 蚕的最后一龄幼虫的丝腺的观察仅在中间的丝腺中显示出荧光。 GFP从旋转阶段周围的中间silkgland细胞分泌，表明GFP移入腺体管腔。 最后，将GFP转染成茧丝，制成含有大量GFP的茧。 因此，通过使用丝胶蛋白基因的启动子区域，可以在中间丝腺中产生重组蛋白质。 此外，在中间丝腺中产生的重组蛋白质容易分泌到中间丝腺的内腔中。

    Inkjet recording device
    9.
    发明授权
    Inkjet recording device 有权
    喷墨记录装置

    公开(公告)号：US08955938B2

    公开(公告)日：2015-02-17

    申请号：US13848165

    申请日：2013-03-21

    申请人： Isao Kobayashi

    发明人： Isao Kobayashi

    IPC分类号： B41J29/38 ,  B41J2/045 ,  B41J2/21

    CPC分类号： B41J2/04501 ,  B41J2/2128 ,  B41J2/2132

    摘要： An inkjet recording device includes: first and second pairs of rollers for conveying a sheet while nipping the sheet; and a recording head disposed between the first and seconds pairs of rollers. The recording head performs an image recording on the sheet by ejecting ink droplets onto the sheet when the sheet is stopped in an intermittent conveyance of the sheet. The recording head performs the image recording in accordance with a first image-recording data, before a trailing end of the sheet passes through the first pair of rollers that is disposed on an upstream side of the second pair of rollers. The recording head performs the image recording in accordance with a second image-recording data which is based on the first image-recording data and which has a higher image resolution than the first image-recording data, after the trailing end of the sheet passes through the first pair of rollers.

    摘要翻译： 喷墨记录装置包括：第一和第二对辊，用于在夹持片材的同时传送片材; 以及设置在第一和第二对辊之间的记录头。 记录头通过在片材的间歇传送中停止片材时将墨滴喷射到片材上来对片材进行图像记录。 记录头根据第一图像记录数据执行图像记录，在纸张的尾端通过设置在第二对辊的上游侧的第一对辊之前。 记录头根据第一图像记录数据执行图像记录，该数据基于第一图像记录数据并且具有比第一图像记录数据更高的图像分辨率，在纸张的尾端通过后 第一对辊子。

    Method for producing reduced metal and slag containing oxidized nonferrous metal using an upgraded coal
    10.
    发明授权
    Method for producing reduced metal and slag containing oxidized nonferrous metal using an upgraded coal 有权
    使用升级煤生产含氧化有色金属的还原金属和炉渣的方法

    公开(公告)号：US08790420B2

    公开(公告)日：2014-07-29

    申请号：US12846425

    申请日：2010-07-29

    申请人： Takao Harada ,  Hidetoshi Tanaka ,  Isao Kobayashi ,  Noriyuki Okuyama ,  Takuo Shigehisa

    发明人： Takao Harada ,  Hidetoshi Tanaka ,  Isao Kobayashi ,  Noriyuki Okuyama ,  Takuo Shigehisa

    IPC分类号： C10L1/32

    CPC分类号： C10L9/02 ,  C21B13/006 ,  C21B13/0066 ,  C21B13/10 ,  C21B13/105 ,  Y02P10/136

    摘要： The present invention provides a method for producing a high-quality reduced metal using an upgraded coal as a carbonaceous material to be incorporated. In the method, coal is first aged by heating in an organic solvent to produce upgraded coal for metallurgy having higher thermal plasticity than that of the coal. Then, a mixture of the upgraded coal for metallurgy and a metal oxide-containing raw material is agglomerated by an agglomerator, and the resultant agglomerates are reduced by heating in a furnace and then melted by further heating to produce a reduced melt. The reduced melt is cooled and solidified in the furnace to produce a reduced solid. The reduced solid is discharged to the outside of the furnace and slag is removed using a screen to recover a metal as a reduced metal.

    摘要翻译： 本发明提供一种使用升级煤作为掺入碳质材料制造高品质还原金属的方法。 在该方法中，首先通过在有机溶剂中加热来生产煤，以生产具有比煤的更高的热可塑性的用于冶金的升级煤。 然后，通过凝聚器将用于冶金的改质煤和含金属氧化物的原料的混合物凝聚，并且通过在炉中加热使所得附聚物减少，然后通过进一步加热熔融以产生还原的熔体。 将还原的熔体在炉中冷却并固化以产生还原的固体。 将还原的固体排出到炉外，并使用筛网去除渣，以回收作为还原金属的金属。