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公开(公告)号：US08865966B2
公开(公告)日：2014-10-21
申请号：US13701946
申请日：2011-05-30
申请人： Iwao Kiyokawa , Yuji Arimatsu , Toshihide Miura , Ryo Kojima , Hideki Sezutsu , Keiro Uchino , Isao Kobayashi , Toshiki Tamura
发明人： Iwao Kiyokawa , Yuji Arimatsu , Toshihide Miura , Ryo Kojima , Hideki Sezutsu , Keiro Uchino , Isao Kobayashi , Toshiki Tamura
CPC分类号： C12N15/8509 , A01K67/033 , A01K67/0335 , A01K67/04 , A01K2217/052 , A01K2227/706 , A01K2267/01 , C12N15/09 , C12P21/02
摘要： Pentameric CRP is produced at a high efficiency by transferring DNA, which encodes monomeric CRP, into a silkworm to thereby construct a transgenic silkworm and then collecting and purifying pentameric CRP that is produced by the transgenic silkworm constructed above.
摘要翻译： 通过将编码单体CRP的DNA转移到蚕中，从而构建转基因蚕，然后收集和纯化由上述构建的转基因蚕产生的五聚体CRP，从而高效生产五聚体CRP。
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2.发明申请METHOD FOR PRODUCING PENTAMERIC CRP, PENTAMERIC CRP-PRODUCING TRANSGENIC SILKWORM AND METHOD FOR CONSTRUCTING SAME, DNA ENCODING CANINE MONOMERIC CRP AND EXPRESSION VECTOR CONTAINING THE DNA 有权生产PENTAMERIC CRP，PENTAMERIC CRP生产转基因丝状细胞的方法及其构建方法，DNA编码犬单核细胞CRP和含有DNA的表达载体公开(公告)号：US20130212717A1
公开(公告)日：2013-08-15
申请号：US13701946
申请日：2011-05-30
申请人： Iwao Kiyokawa , Yuji Arimatsu , Toshihide Miura , Ryo Kojima , Hideki Sezutsu , Keiro Uchino , Isao Kobayashi , Toshiki Tamura
发明人： Iwao Kiyokawa , Yuji Arimatsu , Toshihide Miura , Ryo Kojima , Hideki Sezutsu , Keiro Uchino , Isao Kobayashi , Toshiki Tamura
IPC分类号： C12N15/85 , A01K67/033
CPC分类号： C12N15/8509 , A01K67/033 , A01K67/0335 , A01K67/04 , A01K2217/052 , A01K2227/706 , A01K2267/01 , C12N15/09 , C12P21/02
摘要： Pentameric CRP is produced at a high efficiency by transferring DNA, which encodes monomeric CRP, into a silkworm to thereby construct a transgenic silkworm and then collecting and purifying pentameric CRP that is produced by the transgenic silkworm constructed above.
摘要翻译： 通过将编码单体CRP的DNA转移到蚕中，从而构建转基因蚕，然后收集和纯化由上述构建的转基因蚕产生的五聚体CRP，从而高效生产五聚体CRP。
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公开(公告)号：US08426674B2
公开(公告)日：2013-04-23
申请号：US12521701
申请日：2007-12-28
申请人： Iwao Kiyokawa , Tatsuya Ohashi , Toshihide Miura , Katsuhiro Katayama , Toshiki Tamura , Isao Kobayashi , Hideki Sezutsu
发明人： Iwao Kiyokawa , Tatsuya Ohashi , Toshihide Miura , Katsuhiro Katayama , Toshiki Tamura , Isao Kobayashi , Hideki Sezutsu
IPC分类号： A01K67/04
CPC分类号： C12N9/16
摘要： Silkworms which have (i) a DNA encoding a transcriptional regulator operably linked downstream of a promoter of a DNA encoding a protein specifically expressed in the silk gland and (ii) a DNA encoding TRACP5 operably linked downstream of a target promoter of the transcriptional regulator were produced. The result showed that active TRACP5b was produced from the silkworms. This means that TRACP5 produced from the silk gland of the silkworms undergoes processing in the silk gland that is similar to the processing taking place at bone resorption sites.
摘要翻译： 蚕具有（i）编码转录调节子的DNA，其可操作地连接在编码丝腺特异性表达的蛋白质的DNA的启动子的下游，和（ii）可操作地连接在转录调节子的靶启动子下游的编码TRACP5的DNA） 生产。 结果表明，活性TRACP5b是从蚕生产的。 这意味着从蚕丝腺产生的TRACP5在丝腺中进行加工，类似于在骨吸收部位发生的加工。
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公开(公告)号：US20110239313A1
公开(公告)日：2011-09-29
申请号：US12521701
申请日：2007-12-28
申请人： Iwao Kiyokawa , Tatsuya Ohashi , Toshihide Miura , Katsuhiro Katayama , Toshiki Tamura , Isao Kobayashi , Hideki Sezutsu
发明人： Iwao Kiyokawa , Tatsuya Ohashi , Toshihide Miura , Katsuhiro Katayama , Toshiki Tamura , Isao Kobayashi , Hideki Sezutsu
CPC分类号： C12N9/16
摘要： Silkworms which have (i) a DNA encoding a transcriptional regulator operably linked downstream of a promoter of a DNA encoding a protein specifically expressed in the silk gland and (ii) a DNA encoding TRACP5 operably linked downstream of a target promoter of the transcriptional regulator were produced. The result showed that active TRACP5b was produced from the silkworms. This means that TRACP5 produced from the silk gland of the silkworms undergoes processing in the silk gland that is similar to the processing taking place at bone resorption sites.
摘要翻译： 蚕具有（i）编码转录调节子的DNA，其可操作地连接在编码丝腺特异性表达的蛋白质的DNA的启动子的下游，和（ii）可操作地连接在转录调节子的靶启动子下游的编码TRACP5的DNA） 生产。 结果表明，活性TRACP5b由蚕生产。 这意味着从蚕丝腺产生的TRACP5在丝腺中进行加工，类似于在骨吸收部位发生的加工。
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公开(公告)号：US20120276562A1
公开(公告)日：2012-11-01
申请号：US13502198
申请日：2010-10-13
申请人： Wataru Kikuchi , Momoe Sato , Kenta Noda , Iwao Kiyokawa , Toshihide Miura , Ryo Kojima , Fumio Nomura , Kazuyuki Sogawa
发明人： Wataru Kikuchi , Momoe Sato , Kenta Noda , Iwao Kiyokawa , Toshihide Miura , Ryo Kojima , Fumio Nomura , Kazuyuki Sogawa
IPC分类号： G01N33/566 , C07K16/00 , G01N21/47 , G01N33/545 , G01N21/78
CPC分类号： G01N33/6893 , C07K16/36 , C07K2317/34 , G01N33/5308 , G01N2333/75 , G01N2800/08
摘要： Disclosed is an immunoassay method whereby a 5.9 kDa peptide which results from the degradation of the α-E chain and α chain of human fibrinogens and which is used as a peptide marker for diagnosing hepatic disease can be specifically assayed in a biological sample containing contaminating peptides by bringing antibodies that recognize the N terminal of said peptide marker and antibodies that recognize the C terminal of said peptide marker into contact with said peptide marker, forming immune complexes of said peptide marker and the two antibodies, and immunoassaying the obtained immune complexes.
摘要翻译： 公开了一种免疫测定方法，其中可以在含有污染肽的生物样品中特异性地测定由于人纤维蛋白原的α-E链和α链的降解而产生的并用作诊断肝脏疾病的肽标志物的5.9kDa肽 通过将识别所述肽标记的N末端的抗体和识别所述肽标记的C末端的抗体与所述肽标记接触，形成所述肽标记和两种抗体的免疫复合物，并免疫测定所获得的免疫复合物。
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公开(公告)号：US09017959B2
公开(公告)日：2015-04-28
申请号：US13502198
申请日：2010-10-13
申请人： Wataru Kikuchi , Momoe Sato , Kenta Noda , Iwao Kiyokawa , Toshihide Miura , Ryo Kojima , Fumio Nomura , Kazuyuki Sogawa
发明人： Wataru Kikuchi , Momoe Sato , Kenta Noda , Iwao Kiyokawa , Toshihide Miura , Ryo Kojima , Fumio Nomura , Kazuyuki Sogawa
IPC分类号： G01N33/53 , G01N33/543 , G01N33/68 , C07K16/36
CPC分类号： G01N33/6893 , C07K16/36 , C07K2317/34 , G01N33/5308 , G01N2333/75 , G01N2800/08
摘要： Disclosed is an immunoassay method whereby a 5.9 kDa peptide which results from the degradation of the α-E chain and α chain of human fibrinogens and which is used as a peptide marker for diagnosing hepatic disease can be specifically assayed in a biological sample containing contaminating peptides by bringing antibodies that recognize the N terminal of said peptide marker and antibodies that recognize the C terminal of said peptide marker into contact with said peptide marker, forming immune complexes of said peptide marker and the two antibodies, and immunoassaying the obtained immune complexes.
摘要翻译： 公开了一种免疫测定方法，其中可以在含有污染肽的生物样品中特异性地测定由于人纤维蛋白原的α-E链和α链的降解而产生的并用作诊断肝脏疾病的肽标志物的5.9kDa肽 通过将识别所述肽标记的N末端的抗体和识别所述肽标记的C末端的抗体与所述肽标记接触，形成所述肽标记和两种抗体的免疫复合物，并免疫测定所获得的免疫复合物。
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公开(公告)号：US20090136978A1
公开(公告)日：2009-05-28
申请号：US12275635
申请日：2008-11-21
申请人： Ryo KOJIMA , Yoshiro Sato , Toshihide Miura
发明人： Ryo KOJIMA , Yoshiro Sato , Toshihide Miura
CPC分类号： C12Q1/32
摘要： In measuring phosphoric acid by the use of an enzyme cycling system using a dehydrogenase together with a thio-NADP, a thio-NAD, a reduced thio-NADP or a reduced thio-NAD as a coenzyme, phosphoric acid is measurable in a wide concentration range from a low concentration to a high concentration by measuring phosphoric acid after previous removal of free phosphoric acid in reagent components for the measurement.
摘要翻译： 在通过使用脱氢酶与硫代NADP，硫代NAD，还原性硫代NADP或还原型硫代NAD作为辅酶的酶循环系统测量磷酸时，可以以广泛的浓度测量磷酸 通过在先前除去用于测量的试剂组分中的游离磷酸之后测量磷酸，从低浓度到高浓度范围。
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公开(公告)号：US08440422B2
公开(公告)日：2013-05-14
申请号：US12275635
申请日：2008-11-21
申请人： Ryo Kojima , Yoshiro Sato , Toshihide Miura
发明人： Ryo Kojima , Yoshiro Sato , Toshihide Miura
IPC分类号： C12Q1/32
CPC分类号： C12Q1/32
摘要： In measuring phosphoric acid by the use of an enzyme cycling system using a dehydrogenase together with a thio-NADP, a thio-NAD, a reduced thio-NADP or a reduced thio-NAD as a coenzyme, phosphoric acid is measurable in a wide concentration range from a low concentration to a high concentration by measuring phosphoric acid after previous removal of free phosphoric acid in reagent components for the measurement.
摘要翻译： 在通过使用脱氢酶与硫代NADP，硫代NAD，还原性硫代NADP或还原型硫代NAD作为辅酶的酶循环系统测量磷酸时，可以以广泛的浓度测量磷酸 通过在先前除去用于测量的试剂组分中的游离磷酸之后测量磷酸，从低浓度到高浓度范围。
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9.发明授权Image forming apparatus which conveys unfixed toner images to a fixing device in an undisturbed and stable manner so that images may be formed on both sides of a sheet 失效图像形成装置，其以未受干扰且稳定的方式将未定影的调色剂图像传送到定影装置，使得可以在片材的两面上形成图像
公开(公告)号：US5995797A
公开(公告)日：1999-11-30
申请号：US81513
申请日：1998-05-20
CPC分类号： G03G15/657 , G03G15/2028 , G03G2215/00413 , G03G2215/00586 , G03G2215/2083
摘要： In an image forming apparatus provided with a first image carrying member; a belt-shaped second image carrying member onto which a toner image is transferred from the first image carrying member; a pair of rotatable fixing members forming a nip region for fixing the toner images transferred on the both sides of the sheet; a conveying device for guiding the sheet separated from an end of the belt-shaped second image carrying member to the pair of rotatable fixing members, when the sheet is nipped and conveyed through the nip region of the pair of rotatable fixing members, the belt-shaped second image carrying member and the pair of rotatable fixing members are arranged such that the sheet is conveyed so as to form a loop protruding from a line connecting the entrance of the nip region and the end of the belt-shaped second image carrying member.
摘要翻译： 在具有第一图像承载部件的图像形成装置中， 带状的第二图像承载构件，其上从第一图像承载构件转印调色剂图像; 一对可旋转的固定构件，形成用于固定转印在纸张两侧的调色剂图像的辊隙区域; 所述输送装置用于将所述片材从所述带状第二图像承载构件的一端分离到所述一对可旋转固定构件时，当所述片材被夹持并输送通过所述一对可旋转固定构件的所述夹持区域时， 所述一对可旋转固定构件被布置成使得所述片材被输送以形成从连接所述夹持区域的入口和所述带状第二图像承载构件的端部的线突出的环。
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10.发明授权
公开(公告)号：US5886728A
公开(公告)日：1999-03-23
申请号：US755288
申请日：1996-11-22
申请人： Shuta Hamada , Satoshi Haneda , Hisayoshi Nagase , Hiroyuki Tokimatsu , Masahiro Onodera , Toshihide Miura
发明人： Shuta Hamada , Satoshi Haneda , Hisayoshi Nagase , Hiroyuki Tokimatsu , Masahiro Onodera , Toshihide Miura
CPC分类号： B41J2/4476 , G03G15/04081 , G03G15/326 , H04N1/506
摘要： An image forming apparatus has an image forming body which is rotatable in a subsidiary scanning direction. A plurality of imagewise exposure devices for imagewise exposing the image forming body are arranged to face the image forming body and to be aligned in a straight line in a primary scanning direction perpendicular to the subsidiary scanning direction of the image forming body. A common supporting member is provided for supporting the plurality of exposure devices, and bearing members are provided on first and second ends of the common supporting member. The plurality of exposure devices are: (i) radially arranged on the common supporting member with respect to a rotation axis of the image forming body, and (ii) fixed on the supporting member with respect to the bearing members, and the image forming body is rotatable against the common supporting member through the bearing members.
摘要翻译： 图像形成装置具有能够在副扫描方向上旋转的图像形成体。 多个用于成像曝光图像形成体的图像曝光装置被布置为面对图像形成体并且在垂直于成像体的辅助扫描方向的主扫描方向上以直线排列。 设置有用于支撑多个曝光装置的公共支撑构件，并且在公共支撑构件的第一和第二端上设置有支承构件。 多个曝光装置是：（i）相对于图像形成体的旋转轴径向地布置在公共支撑构件上，和（ii）相对于支承构件固定在支撑构件上，并且图像形成体 通过轴承部件能够相对于公共支撑部件转动。
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