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    Method for production of TRACP5b
    3.
    发明授权
    Method for production of TRACP5b 有权
    生产TRACP5b的方法

    公开(公告)号：US08426674B2

    公开(公告)日：2013-04-23

    申请号：US12521701

    申请日：2007-12-28

    申请人： Iwao Kiyokawa ,  Tatsuya Ohashi ,  Toshihide Miura ,  Katsuhiro Katayama ,  Toshiki Tamura ,  Isao Kobayashi ,  Hideki Sezutsu

    发明人： Iwao Kiyokawa ,  Tatsuya Ohashi ,  Toshihide Miura ,  Katsuhiro Katayama ,  Toshiki Tamura ,  Isao Kobayashi ,  Hideki Sezutsu

    IPC分类号： A01K67/04

    CPC分类号： C12N9/16

    摘要： Silkworms which have (i) a DNA encoding a transcriptional regulator operably linked downstream of a promoter of a DNA encoding a protein specifically expressed in the silk gland and (ii) a DNA encoding TRACP5 operably linked downstream of a target promoter of the transcriptional regulator were produced. The result showed that active TRACP5b was produced from the silkworms. This means that TRACP5 produced from the silk gland of the silkworms undergoes processing in the silk gland that is similar to the processing taking place at bone resorption sites.

    摘要翻译： 蚕具有（i）编码转录调节子的DNA，其可操作地连接在编码丝腺特异性表达的蛋白质的DNA的启动子的下游，和（ii）可操作地连接在转录调节子的靶启动子下游的编码TRACP5的DNA） 生产。 结果表明，活性TRACP5b是从蚕生产的。 这意味着从蚕丝腺产生的TRACP5在丝腺中进行加工，类似于在骨吸收部位发生的加工。

    METHOD FOR PRODUCTION OF TRACP5b
    4.
    发明申请
    METHOD FOR PRODUCTION OF TRACP5b 有权
    生产TRACP5b的方法

    公开(公告)号：US20110239313A1

    公开(公告)日：2011-09-29

    申请号：US12521701

    申请日：2007-12-28

    申请人： Iwao Kiyokawa ,  Tatsuya Ohashi ,  Toshihide Miura ,  Katsuhiro Katayama ,  Toshiki Tamura ,  Isao Kobayashi ,  Hideki Sezutsu

    发明人： Iwao Kiyokawa ,  Tatsuya Ohashi ,  Toshihide Miura ,  Katsuhiro Katayama ,  Toshiki Tamura ,  Isao Kobayashi ,  Hideki Sezutsu

    IPC分类号： C12P21/00 ,  C12N15/85 ,  A01K67/04

    CPC分类号： C12N9/16

    摘要： Silkworms which have (i) a DNA encoding a transcriptional regulator operably linked downstream of a promoter of a DNA encoding a protein specifically expressed in the silk gland and (ii) a DNA encoding TRACP5 operably linked downstream of a target promoter of the transcriptional regulator were produced. The result showed that active TRACP5b was produced from the silkworms. This means that TRACP5 produced from the silk gland of the silkworms undergoes processing in the silk gland that is similar to the processing taking place at bone resorption sites.

    摘要翻译： 蚕具有（i）编码转录调节子的DNA，其可操作地连接在编码丝腺特异性表达的蛋白质的DNA的启动子的下游，和（ii）可操作地连接在转录调节子的靶启动子下游的编码TRACP5的DNA） 生产。 结果表明，活性TRACP5b由蚕生产。 这意味着从蚕丝腺产生的TRACP5在丝腺中进行加工，类似于在骨吸收部位发生的加工。

    Methods for producing proteins using silkworm middle silk gland-specific gene expression system
    5.
    发明申请
    Methods for producing proteins using silkworm middle silk gland-specific gene expression system 有权
    使用蚕丝绸腺特异性基因表达系统生产蛋白质的方法

    公开(公告)号：US20060070132A1

    公开(公告)日：2006-03-30

    申请号：US11085838

    申请日：2005-03-21

    申请人： Toshiki Tamura ,  Hideki Sezutsu ,  Isao Kobayashi ,  Katsura Kojima ,  Toshio Kanda ,  Keiro Uchino

    发明人： Toshiki Tamura ,  Hideki Sezutsu ,  Isao Kobayashi ,  Katsura Kojima ,  Toshio Kanda ,  Keiro Uchino

    IPC分类号： A01K67/033

    CPC分类号： C12N15/8509 ,  A01K67/0333 ,  A01K67/04 ,  A01K2217/05 ,  A01K2227/706 ,  A01K2267/01 ,  A01K2267/0393

    摘要： Transgenic silkworms comprising GFP whose expression is regulated by the sericin gene promoter were produced. Observation of the silk glands of the last instar larvae of the silkworms showed fluorescence only in the middle silk glands. GFP was secreted from middle silkgland cells from around the spinning stage, indicating that GFP moved into the gland lumen. Finally, GFP was spun into cocoon filaments, and cocoons containing large amounts of GFP were produced. Thus, by using the promoter region of the sericin gene, recombinant proteins can be produced in the middle silk glands. Furthermore, the recombinant proteins produced in the middle silk glands were readily secreted into the lumen of the middle silk glands.

    摘要翻译： 产生包含GFP的转基因蚕，其表达受丝胶蛋白基因启动子调节。 蚕的最后一龄幼虫的丝腺的观察仅在中间的丝腺中显示出荧光。 GFP从旋转阶段周围的中间silkgland细胞分泌，表明GFP移入腺体管腔。 最后，将GFP转染成茧丝，制成含有大量GFP的茧。 因此，通过使用丝胶蛋白基因的启动子区域，可以在中间丝腺中产生重组蛋白质。 此外，在中间丝腺中产生的重组蛋白质容易分泌到中间丝腺的内腔中。

    Modular plug for a signal transmission cable
    9.
    发明授权
    Modular plug for a signal transmission cable 失效
    用于信号传输电缆的模块化插头

    公开(公告)号：US6123572A

    公开(公告)日：2000-09-26

    申请号：US419223

    申请日：1999-10-15

    申请人： Takuji Ishii ,  Toshiki Tamura ,  Masahito Watanabe

    发明人： Takuji Ishii ,  Toshiki Tamura ,  Masahito Watanabe

    IPC分类号： H01R13/50 ,  H01R13/506 ,  H01R13/58 ,  H01R11/20 ,  H01R13/14

    CPC分类号： H01R13/5829 ,  H01R13/6463 ,  H01R13/501 ,  H01R13/506

    摘要： A modular plug includes a dielectric housing, a plurality of terminals, and a signal transmission cable. The dielectric housing has a cable insertion end, a mating end opposite to the cable insertion end, a lower body portion, and an upper lid portion connected to the lower body portion. The lower body portion extends from the cable insertion end to the mating end. The lower body portion has a plurality of terminal grooves formed adjacent to the mating end, and a cable receiving space extending from the cable insertion end adjacent to the terminal grooves. The upper lid portion is operable relative to the lower body portion to expose the cable receiving space. The terminals are inserted into the cable receiving space through the terminal grooves. The signal transmission cable is received in the cable receiving space, and has a plurality of conductive wires that extend adjacent to the terminal grooves, respectively, and that are connected electrically to the terminals. The upper lid portion lies over the cable receiving space without extending to the terminal grooves, and has a protrusion projecting into the cable receiving space to press the signal transmission cable against the lower body portion.

    摘要翻译： 模块化插头包括绝缘壳体，多个端子和信号传输电缆。 绝缘壳体具有电缆插入端，与电缆插入端相对的配合端，下主体部分和连接到下主体部分的上盖部分。 下主体部分从电缆插入端延伸到配合端。 下主体部分具有邻近配合端形成的多个端子槽，以及从电缆插入端部延伸到电缆槽的相邻端子槽的电缆接收空间。 上盖部分可相对于下主体部分操作以暴露电缆接收空间。 端子通过端子槽插入电缆接收空间。 信号传输电缆被接收在电缆接收空间中，并且具有分别与端子槽相邻延伸并且与端子电连接的多条导线。 上盖部分位于电缆接收空间之上，而不延伸到端子槽，并且具有突出到电缆接收空间中的突起，以将信号传输电缆按压在下主体部分上。

    Process for producing physiologically active protein using genetically modified silkworm
    10.
    发明授权
    Process for producing physiologically active protein using genetically modified silkworm 失效
    使用转基因蚕生产生理活性蛋白的方法

    公开(公告)号：US07659112B2

    公开(公告)日：2010-02-09

    申请号：US10506327

    申请日：2003-03-06

    申请人： Shingo Hiramatsu ,  Takashi Tanaka ,  Katsushige Yamada ,  Toshiki Tamura

    发明人： Shingo Hiramatsu ,  Takashi Tanaka ,  Katsushige Yamada ,  Toshiki Tamura

    IPC分类号： C12N15/00 ,  C12P21/00

    CPC分类号： C12N15/8509 ,  A01K67/033 ,  A01K67/0335 ,  A01K67/04 ,  A01K2217/05 ,  A01K2267/01 ,  C07K14/52

    摘要： The present invention provides a genetic engineering material for insects that enables a target protein to be purified easily, without requiring the use of recombinant baculovirus, while simultaneously providing a process for producing exogenous protein using that genetic engineering material. A gene recombinant silkworm is obtained by inserting an exogenous protein gene such as a cytokine gene coupled to a promoter that functions in silk glands into a silkworm chromosome. An exogenous protein such as a cytokine is then extracted and purified from the silk glands or cocoon of that silkworm or its offspring. A large amount of exogenous protein can be produced within silk gland cells, outside silk gland cells or in silk thread or a cocoon by inserting an expression gene cassette, in which the DNA sequence of the 3′ terminal portion and the DNA sequence of the 5′ terminal portion of fibroin H chain gene are fused to the exogenous protein gene, into silk gland cells and so forth.

    摘要翻译： 本发明提供了一种用于昆虫的遗传工程材料，其能够容易地纯化靶蛋白，而不需要使用重组杆状病毒，同时提供使用该遗传工程材料生产外源蛋白的方法。 通过将外源蛋白质基因例如与在丝腺中起作用的启动子偶联的细胞因子基因插入蚕染色体而获得基因重组蚕。 然后从蚕丝或其后代的丝腺或茧中提取和纯化外源蛋白，如细胞因子。 通过插入表达基因盒，可以在丝腺细胞，丝腺细胞外或丝线或茧中产生大量外源蛋白，其中3'末端部分的DNA序列和5'端的DNA序列 “丝心蛋白H链基因的末端部分融合到外源蛋白基因，进入丝腺细胞等。