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公开(公告)号：US06855365B2
公开(公告)日：2005-02-15
申请号：US09866379
申请日：2001-05-24
申请人： Jay M. Short , Keith A. Kretz , Kevin A. Gray , Nelson Robert Barton , James B. Garrett , Eileen O'Donoghue , Eric J. Mathur
发明人： Jay M. Short , Keith A. Kretz , Kevin A. Gray , Nelson Robert Barton , James B. Garrett , Eileen O'Donoghue , Eric J. Mathur
IPC分类号： A01H5/00 , A01K67/027 , A21D8/04 , A23J3/14 , A23K1/14 , A23K1/16 , A23K1/165 , A23L1/015 , A23L1/03 , A23L1/105 , A23L1/20 , A23L1/211 , A23L1/30 , A61K38/00 , C07K16/40 , C12M1/00 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/16 , C12N15/09 , C12P21/08 , C12Q1/42 , G01N33/53 , G01N33/566 , C07H21/04 , C12N1/20 , C12N15/00 , C12Q1/34
CPC分类号： A21D8/042 , A01K2217/05 , A23J3/14 , A23K10/30 , A23K20/189 , A23L5/25 , A23L7/107 , A23L11/33 , A23L29/06 , A23V2002/00 , A61K38/00 , C12N9/16 , C12Y301/03008 , C12Y301/03026 , A23V2300/21
摘要： A purified and modified phytase enzyme from Escherichia coli K12 appA phytase is provided. The enzyme has phytase activity and improved thermal tolerance as compared with the wild-type enzyme. In addition, the enzyme has improved protease stability at low pH. Glycosylation of the modified phytase provided a further improved enzyme having improved thermal tolerance and protease stability. The enzyme can be produced from native or recombinant host cells and can be used to aid in the digestion of phytate where desired. In particular, the phytase of the present invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients.
摘要翻译： 提供了来自大肠杆菌K12 appA植酸酶的纯化和修饰的植酸酶。 与野生型酶相比，该酶具有植酸酶活性和改善的耐热性。 此外，该酶在低pH下具有改善的蛋白酶稳定性。 修饰的植酸酶的糖基化提供了具有改善的热耐受性和蛋白酶稳定性的进一步改进的酶。 该酶可以由天然或重组宿主细胞产生，并且可以用于有助于在需要时消化植酸盐。 特别地，本发明的植酸酶可用于食品中以改善富含植酸盐的成分的摄食量。
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2.发明授权Phytases, nucleic acids encoding them and methods of making and using them 有权植酸酶，编码它们的核酸和制备和使用它们的方法公开(公告)号：US07432097B2
公开(公告)日：2008-10-07
申请号：US10933115
申请日：2004-09-01
申请人： Jay M. Short , Keith A. Kretz , Kevin A. Gray , Nelson Robert Barton , James B. Garrett , Eileen O'Donoghue , William Baum , Dan E. Robertson , Paul Zorner
发明人： Jay M. Short , Keith A. Kretz , Kevin A. Gray , Nelson Robert Barton , James B. Garrett , Eileen O'Donoghue , William Baum , Dan E. Robertson , Paul Zorner
IPC分类号： C12N11/00 , C12N9/16 , A61K38/46 , C07K14/00 , C12N15/00 , C12N1/21 , C12N5/10 , C12P21/00 , C12Q1/44 , C07H21/00
CPC分类号： C12N9/16 , A01K2217/05 , A21D8/042 , A23J3/14 , A23K10/30 , A23K20/189 , A23L5/25 , A23L7/107 , A23L11/33 , A23L29/06 , A23V2002/00 , A61K33/00 , A61K36/00 , A61K38/00 , A61K45/06 , C12Y301/03008 , C12Y301/03026 , Y02P60/877 , A23V2300/21
摘要： In one aspect, the invention provides a purified and modified phytase enzyme from Escherichia coli K12 appA phytase. The enzyme has phytase activity and improved thermal tolerance as compared with the wild-type enzyme. In addition, the enzyme has improved protease stability at low pH. Glycosylation of the modified phytase provided a further improved enzyme having improved thermal tolerance and protease stability. The enzyme can be produced from native or recombinant host cells and can be used to aid in the digestion of phytate where desired. In one aspect, the phytase of the present invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients.
摘要翻译： 一方面，本发明提供了来自大肠杆菌K12 appA植酸酶的纯化和修饰的植酸酶。 与野生型酶相比，该酶具有植酸酶活性和改善的耐热性。 此外，该酶在低pH下具有改善的蛋白酶稳定性。 修饰的植酸酶的糖基化提供了具有改善的热耐受性和蛋白酶稳定性的进一步改进的酶。 该酶可以由天然或重组宿主细胞产生，并且可以用于有助于在需要时消化植酸盐。 一方面，本发明的植酸酶可用于食品中以改善富含植酸盐的成分的摄食量。
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公开(公告)号：US07452706B2
公开(公告)日：2008-11-18
申请号：US10034985
申请日：2001-12-21
申请人： Jay M. Short , Keith A. Kretz , Dan E. Robertson
发明人： Jay M. Short , Keith A. Kretz , Dan E. Robertson
IPC分类号： C12N9/16 , C12Q1/44 , A23J1/00 , A61K38/46 , C07K14/00 , C12P21/02 , C12N15/00 , C12N1/21 , C12N1/15 , C12N5/10 , C12N1/19 , C07H21/00
CPC分类号： A21D8/042 , A01K2217/05 , A23J3/14 , A23K10/30 , A23K20/189 , A23L5/25 , A23L7/107 , A23L11/33 , A23L29/06 , A23V2002/00 , A61K38/00 , C12N9/16 , C12Y301/03008 , C12Y301/03026 , A23V2300/21
摘要： A purified recombinant phytase enzyme derived from Escherichia coli B. The enzyme has a molecular weight of about 47.1 kilodaltons and has phytase activity. The enzyme can be produced from native or recombinant host cells and can be used to aid in the digestion of phytate where desired. In particular, the phytase of the present invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients.
摘要翻译： 来自大肠杆菌B的纯化重组植酸酶。该酶具有约47.1千道尔顿的分子量并具有植酸酶活性。 该酶可以由天然或重组宿主细胞产生，并且可以用于有助于在需要时消化植酸盐。 特别地，本发明的植酸酶可用于食品中以改善富含植酸盐的成分的摄食量。
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公开(公告)号：US06720014B1
公开(公告)日：2004-04-13
申请号：US09580515
申请日：2000-05-25
申请人： Jay M. Short , Keith A. Kretz
发明人： Jay M. Short , Keith A. Kretz
IPC分类号： C12N916
CPC分类号： A21D8/042 , A01K2217/05 , A23J3/14 , A23K10/30 , A23K20/189 , A23L5/25 , A23L7/107 , A23L11/33 , A23L29/06 , A23V2002/00 , A61K38/00 , C12N9/16 , C12Y301/03008 , C12Y301/03026 , A23V2300/21
摘要： A purified recombinant phytase enzyme derived from Escherichia coli B. The enzyme has a molecular light of about 47.1 kilodaltons and has phytase activity. The enzyme can be produced from native or recombinant host cells and can be used to aid in the digestion of phytate where desired. In particular, the phytase of the present invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients.
摘要翻译： 来自大肠杆菌B的纯化的重组植酸酶。该酶具有约47.1千道尔顿的分子量并具有植酸酶活性。 该酶可以由天然或重组宿主细胞产生，并且可以用于有助于在需要时消化植酸盐。 特别地，本发明的植酸酶可用于食品中以改善富含植酸盐的成分的摄食量。
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公开(公告)号：US07232677B2
公开(公告)日：2007-06-19
申请号：US09777566
申请日：2001-02-05
申请人： Jay M. Short , Keith A. Kretz
发明人： Jay M. Short , Keith A. Kretz
CPC分类号： C12N15/8242 , A01K2217/05 , A21D8/042 , A23K20/189 , A23L5/25 , A23L7/101 , A23L11/00 , A23L11/33 , A23L29/06 , A23L33/17 , A23L33/185 , A61K38/00 , C12C5/004 , C12G2200/15 , C12N9/16 , C12N15/8243 , C12N15/8245 , C12Y301/03026
摘要： The invention provides a purified or recombinant phytase enzyme (SEQ ID NO:2) initially derived from Escherichia coli B. The enzyme has a molecular weight of about 47.1 kilodaltons and has phytase activity (SEQ ID NO:2). The enzyme can be produced from native or recombinant host cells and can be used to aid in the digestion of phytate where desired. In particular, the phytase of the present invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients.
摘要翻译： 本发明提供了最初衍生自大肠杆菌B的纯化或重组植酸酶（SEQ ID NO：2）。该酶具有约47.1千道尔顿的分子量并具有植酸酶活性（SEQ ID NO：2）。 该酶可以由天然或重组宿主细胞产生，并且可以用于有助于在需要时消化植酸盐。 特别地，本发明的植酸酶可用于食品中以改善富含植酸盐的成分的摄食量。
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公开(公告)号：US06183740B2
公开(公告)日：2001-02-06
申请号：US09318528
申请日：1999-05-25
申请人： Jay M. Short , Keith A. Kretz
发明人： Jay M. Short , Keith A. Kretz
IPC分类号： A61K3846
CPC分类号： C12N15/8242 , A01K2217/05 , A21D8/042 , A23K20/189 , A23L5/25 , A23L7/101 , A23L11/00 , A23L11/33 , A23L29/06 , A23L33/17 , A23L33/185 , A61K38/00 , C12C5/004 , C12G2200/15 , C12N9/16 , C12N15/8243 , C12N15/8245 , C12Y301/03026
摘要： A purified recombinant phytase enzyme derived from Escherichia coli B. The enzyme has a molecular weight of about 47.1 kilodaltons and has phytase activity (SEQ ID NO:2). The enzyme can be produced from native or recombinant host cells and can be used to aid in the digestion of phytate where desired. In particular, the phytase of the present invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients.
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