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公开(公告)号：US6132992A
公开(公告)日：2000-10-17
申请号：US539436
申请日：1995-10-05
申请人： Jeffrey A. Ledbetter , Lisa K. Gilliland , Martha S. Hayden , Peter S. Linsley , Jurgen Bajorath , H. Perry Fell
发明人： Jeffrey A. Ledbetter , Lisa K. Gilliland , Martha S. Hayden , Peter S. Linsley , Jurgen Bajorath , H. Perry Fell
IPC分类号： C07K14/705 , A61K38/00 , C07K14/00 , C07K14/16 , C07K14/715 , C07K16/00 , C07K16/28 , C07K16/30 , C07K16/46 , C07K19/00 , C12N5/10 , C12N15/09 , C12N15/13 , C12N15/62 , C12P21/02 , C12P21/08 , C12R1/91 , G01N33/563 , G01N33/569 , G01N33/574 , C12N15/85
CPC分类号： C07K14/005 , C07K14/70521 , C07K14/7155 , C07K16/00 , C07K16/2809 , C07K16/2818 , C07K16/2827 , C07K16/30 , C07K16/468 , C12N15/62 , G01N33/563 , G01N33/56972 , G01N33/57492 , A61K2039/505 , A61K38/00 , C07K2317/31 , C07K2317/34 , C07K2317/622 , C07K2317/732 , C07K2317/734 , C07K2317/74 , C07K2319/00 , C07K2319/02 , C07K2319/30 , C07K2319/43 , C12N2740/16122 , G01N2333/705
摘要： The present invention provides an expression vector encoding monospecific or bispecific fusion protein. In one embodiment the expression vector encodes a monospecific fusion protein, which vector comprises a recombinant monospecific single chain cassette comprising a DNA sequence encoding a first binding domain capable of binding a cell surface antigen. In another embodiment the expression vector encodes a bispecific fusion protein, which vector comprises a recombinant bispecific single chain cassette comprising a DNA sequence encoding a first binding domain capable of binding a cell surface antigen and a DNA sequence encoding a second binding domain capable of binding a cell surface antigen, each domain capable of binding a different antigen. The present invention also provides a method for producing a biologically active monospecific or bispecific fusion protein in a mammalian cell. This method comprises: (a) transfecting the mammalian cell with the recombinant expression vector of the invention; (b) culturing the mammalian cell so transfected in step (a); and (c) recovering the biologically active bispecific fusion protein so as produced by the cultured mammalian cell.
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2.发明授权Expression vectors encoding bispecific fusion proteins and methods of producing biologically active bispecific fusion proteins in a mammalian cell 失效编码双特异性融合蛋白的表达载体和在哺乳动物细胞中产生生物活性双特异性融合蛋白的方法公开(公告)号：US07915395B2
公开(公告)日：2011-03-29
申请号：US10283610
申请日：2002-10-29
申请人： Jeffrey A. Ledbetter , Martha S. Hayden , Peter S. Linsley , Jurgen Bajorath , H. Perry Fell , Lisa K. Gilliland
发明人： Jeffrey A. Ledbetter , Martha S. Hayden , Peter S. Linsley , Jurgen Bajorath , H. Perry Fell , Lisa K. Gilliland
CPC分类号： C07K14/005 , A61K38/00 , A61K2039/505 , C07K14/70521 , C07K14/7155 , C07K16/00 , C07K16/2809 , C07K16/2818 , C07K16/2827 , C07K16/30 , C07K16/468 , C07K2317/31 , C07K2317/34 , C07K2317/622 , C07K2317/732 , C07K2317/734 , C07K2317/74 , C07K2319/00 , C07K2319/02 , C07K2319/30 , C07K2319/43 , C12N15/62 , C12N2740/16122 , G01N33/563 , G01N33/56972 , G01N33/57492 , G01N2333/705
摘要： The present invention provides an expression vector encoding monospecific or bispecific fusion protein. In one embodiment the expression vector encodes a monospecific fusion protein, which vector comprises a recombinant monospecific single chain cassette comprising a DNA sequence encoding a first binding domain capable of binding a cell surface antigen. In another embodiment the expression vector encodes a bispecific fusion protein, which vector comprises a recombinant bispecific single chain cassette comprising a DNA sequence encoding a first binding domain capable of binding a cell surface antigen and a DNA sequence encoding a second binding domain capable of binding a cell surface antigen, each domain capable of binding a different antigen. The present invention also provides a method for producing a biologically active monospecific or bispecific fusion protein in a mammalian cell.
摘要翻译： 本发明提供编码单特异性或双特异性融合蛋白的表达载体。 在一个实施方案中，表达载体编码单特异性融合蛋白，该载体包含重组单特异性单链盒，其包含编码能够结合细胞表面抗原的第一结合结构域的DNA序列。 在另一个实施方案中，表达载体编码双特异性融合蛋白，该载体包含重组双特异性单链盒，其包含编码能够结合细胞表面抗原的第一结合结构域的DNA序列和编码能够结合细胞表面抗原的第二结合结构域的DNA序列 细胞表面抗原，每个结构域能够结合不同的抗原。 本发明还提供了在哺乳动物细胞中产生生物活性单特异性或双特异性融合蛋白的方法。
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3.发明授权Expression vectors encoding bispecific fusion proteins and methods of producing biologically active bispecific fusion proteins in a mammalian cell 失效编码双特异性融合蛋白的表达载体和在哺乳动物细胞中产生生物活性双特异性融合蛋白的方法
公开(公告)号：US5637481A
公开(公告)日：1997-06-10
申请号：US121054
申请日：1993-09-13
申请人： Jeffrey A. Ledbetter , Lisa K. Gilliland , Martha S. Hayden , Peter S. Linsley , Jurgen Bajorath , H. Perry Fell
发明人： Jeffrey A. Ledbetter , Lisa K. Gilliland , Martha S. Hayden , Peter S. Linsley , Jurgen Bajorath , H. Perry Fell
IPC分类号： C07K14/705 , A61K38/00 , C07K14/00 , C07K14/16 , C07K14/715 , C07K16/00 , C07K16/28 , C07K16/30 , C07K16/46 , C07K19/00 , C12N5/10 , C12N15/09 , C12N15/13 , C12N15/62 , C12P21/02 , C12P21/08 , C12R1/91 , G01N33/563 , G01N33/569 , G01N33/574 , C12N15/79 , C12P21/00
CPC分类号： C07K14/005 , C07K14/70521 , C07K14/7155 , C07K16/00 , C07K16/2809 , C07K16/2818 , C07K16/2827 , C07K16/30 , C07K16/468 , C12N15/62 , G01N33/563 , G01N33/56972 , G01N33/57492 , A61K2039/505 , A61K38/00 , C07K2317/31 , C07K2317/34 , C07K2317/622 , C07K2317/732 , C07K2317/734 , C07K2317/74 , C07K2319/00 , C07K2319/02 , C07K2319/30 , C07K2319/43 , C12N2740/16122 , G01N2333/705
摘要： The present invention provides an expression vector encoding monospecific or bispecific fusion protein. In one embodiment the expression vector encodes a monospecific fusion protein, which vector comprises a recombinant monospecific single chain cassette comprising a DNA sequence encoding a first binding domain capable of binding a cell surface antigen.
摘要翻译： 本发明提供编码单特异性或双特异性融合蛋白的表达载体。 在一个实施方案中，表达载体编码单特异性融合蛋白，该载体包含重组单特异性单链盒，其包含编码能够结合细胞表面抗原的第一结合结构域的DNA序列。
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4.发明授权Expression vectors encoding bispecific fusion proteins and methods of producing biologically active bispecific fusion proteins in a mammalian cell 失效编码双特异性融合蛋白的表达载体和在哺乳动物细胞中产生生物活性双特异性融合蛋白的方法公开(公告)号：US06482919B2
公开(公告)日：2002-11-19
申请号：US09813659
申请日：2001-03-21
申请人： Jeffrey A. Ledbetter , Martha S. Hayden , Peter S. Linsley , Jurgen Bajorath , H. Perry Fell , Lisa K. Gilliland
发明人： Jeffrey A. Ledbetter , Martha S. Hayden , Peter S. Linsley , Jurgen Bajorath , H. Perry Fell , Lisa K. Gilliland
IPC分类号： C07K1628
CPC分类号： C07K14/005 , A61K38/00 , A61K2039/505 , C07K16/2809 , C07K16/30 , C07K16/468 , C07K2317/31 , C07K2317/34 , C07K2317/622 , C07K2317/732 , C07K2317/734 , C07K2317/74 , C07K2319/00 , C07K2319/02 , C07K2319/30 , C07K2319/43 , C12N15/62 , C12N2740/16122 , G01N33/563 , G01N33/56972 , G01N33/57492 , G01N2333/705
摘要： The present invention provides novel soluble CTLA4Ig molecules having modified Ig domains.
摘要翻译： 本发明提供具有修饰的Ig结构域的新的可溶性CTLA4Ig分子。
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5.发明授权Expression of DNA sequences encoding a thermally stable cytosine deaminase from saccharomyces 失效表达编码来自酵母菌的热稳定胞嘧啶脱氨酶的DNA序列
公开(公告)号：US5338678A
公开(公告)日：1994-08-16
申请号：US531646
申请日：1990-06-01
IPC分类号： C12N15/09 , C12N1/00 , C12N9/78 , C12N15/55 , C12R1/865 , C12N5/00 , C07H15/12 , C12N1/20 , C12N15/00
CPC分类号： C12N9/78
摘要： Thermally stable cytosine deaminase (CDase), and the gene coding therefor, is disclosed as well as methods of isolating, purifying, and recombinantly producing the same. The thermally stable CDase can be isolated from Saccharomyces cerevisiae. The yeast isolated enzyme has a molecular weight of approximately 32 kDa, as determined by gel filtration chromatography, and is composed of two subunits, each with a molecular weight of about 17 kDa. Thermally stable yeast CDase so purified shows no significant sequence homology with other known sequenced proteins.
摘要翻译： 公开了热稳定的胞嘧啶脱氨酶（CDase）及其编码基因，以及分离，纯化和重组产生其的方法。 热稳定的CDase可以从酿酒酵母中分离出来。 酵母分离的酶具有通过凝胶过滤色谱法测定的约32kDa的分子量，由两个分子量约17kDa的亚基组成。 如此纯化的热稳定酵母CDase与其他已知的测序蛋白质没有显着的序列同源性。
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公开(公告)号：US5545548A
公开(公告)日：1996-08-13
申请号：US262238
申请日：1994-06-20
CPC分类号： C12N9/78
摘要： Thermally stable cytosine deaminase (CDase), and the gene coding therefor, is disclosed as well as methods of isolating, purifying, and recombinantly producing the same. The thermally stable CDase can be isolated from Saccharomyces cerevisiae. The yeast isolated enzyme has a molecular weight of approximately 32 kDa, as determined by gel filtration chromatography, and is composed of two subunits, each with a molecular weight of about 17 kDa. Thermally stable yeast CDase so purified shows no significant sequence homology with other known sequenced proteins.
摘要翻译： 公开了热稳定的胞嘧啶脱氨酶（CDase）及其编码基因，以及分离，纯化和重组产生其的方法。 热稳定的CDase可以从酿酒酵母中分离出来。 酵母分离的酶具有通过凝胶过滤色谱法测定的约32kDa的分子量，由两个分子量约17kDa的亚基组成。 如此纯化的热稳定酵母CDase与其他已知的测序蛋白质没有显着的序列同源性。
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