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    Nanogel-based contrast agents for optical molecular imaging
    1.
    发明申请
    Nanogel-based contrast agents for optical molecular imaging 审中-公开
    基于Nanogel的光学分子成像造影剂

    公开(公告)号：US20070237821A1

    公开(公告)日：2007-10-11

    申请号：US11401343

    申请日：2006-04-10

    申请人： Jeffrey Leon ,  James Bennett ,  Tiecheng Qiao ,  John Harder ,  Thomas Mourey ,  Gary Slater ,  Lijun Dai

    发明人： Jeffrey Leon ,  James Bennett ,  Tiecheng Qiao ,  John Harder ,  Thomas Mourey ,  Gary Slater ,  Lijun Dai

    IPC分类号： A61K9/14

    CPC分类号： A61K49/0093 ,  A61K47/6903 ,  A61K47/6933 ,  A61K49/0032 ,  A61K49/0073 ,  B82Y5/00

    摘要： The present invention relates to a nanogel comprising a polymer network of repetitive, crosslinked, ethylenically unsaturated monomers of Formula I: (X)m-(Y)n-(Z)o  Formula I wherein X is a water-soluble monomer containing ionic or hydrogen bonding moieties; Y is a water-soluble macromonomer containing repetitive hydrophilic units bound to a polymerizeable ethylenically unsaturated group; Z is a multifunctional crosslinking monomer; m ranges from 50-90 mol %; n ranges from 2-30 mol %; and o range from 1-15 mol % and a method for preparing a nanogel comprising preparing a header composition of a mixture of monomers X, Y, and Z, and a first portion of initiators in water; preparing a reactor composition of a second portion initiators, surfactant, and water; bringing the reactor composition to the polymerization temperature; holding the reactor composition at the polymerization temperature, and adding the header composition to the reactor composition to form a nanogel of Formula I.

    摘要翻译： 本发明涉及纳米凝胶，其包含式I的重复，交联，烯键式不饱和单体的聚合物网络：<？在线公式描述=“在线式”末端=“铅”→（X）m- （Y）n-（Z）o式I <？in-line-formula description =“In-line Formulas”end =“tail”？>其中X是含有离子或氢键结构部分的水溶性单体; Y是含有与可聚合烯属不饱和基团结合的重复亲水单元的水溶性大分子单体; Z是多功能交联单体; m范围为50-90摩尔％; n范围为2-30mol％; 其范围为1-15mol％，制备纳米凝胶的方法包括制备单体X，Y和Z的混合物的头部组合物和在水中的第一部分引发剂; 制备第二部分引发剂，表面活性剂和水的反应器组合物; 使反应器组成达到聚合温度; 将反应器组合物保持在聚合温度，并将集管组合物加入到反应器组合物中以形成式I的纳米凝胶。

    Methods for separation of polymeric compounds
    2.
    发明申请
    Methods for separation of polymeric compounds 有权
    聚合物分离方法

    公开(公告)号：US20060177840A1

    公开(公告)日：2006-08-10

    申请号：US11241990

    申请日：2005-10-04

    申请人： Gary Slater ,  Laurette McCormick ,  Annelise Barron ,  Robert Meagher

    发明人： Gary Slater ,  Laurette McCormick ,  Annelise Barron ,  Robert Meagher

    IPC分类号： C12Q1/68 ,  C07K1/26

    CPC分类号： C07K1/13 ,  C07K1/26 ,  C12Q1/6816 ,  C12Q2525/185 ,  C12Q2565/125

    摘要： Recently two techniques using free solution electrophoresis to separate charged-uncharged polymer conjugates have proven successful: End Labeled Free Solution Electrophoresis (ELFSE) for DNA sequencing, and Free Solution Conjugate Electrophoresis (FSCE) for molar mass profiling of uncharged polymers. Previous attempts have been made to analyze experimental data generated by these new techniques for the electrophoresis of molecules with varying charge distributions. However, the importance of the ends of the polymers in determining the polymer's overall mobility was neglected in previous work. Through a careful investigation and a reanalysis of the experimental data, it is determined here that this “end effect” critically impacts the behavior of polymers and charged-uncharged polymer conjugates during electrophoresis. In this way, the invention provides for methods that exploit this “end effect” for the separation of polymeric molecules on the basis of size, including for example DNA separation and sequencing techniques.

    摘要翻译： 最近，使用自由溶液电泳分离带电荷的聚合物共轭物的两种技术已被证明是成功的：用于DNA测序的末端标记的游离溶液电泳（ELFSE）和用于不带电聚合物的摩尔质谱分析的游离溶液共轭电泳（FSCE）。 以前曾经尝试分析由这些新技术产生的用于具有不同电荷分布的分子的电泳产生的实验数据。 然而，聚合物末端在确定聚合物整体流动性方面的重要性在以前的工作中被忽略。 通过对实验数据的仔细研究和重新分析，这里确定，这种“最终效应”严重影响聚合物和带电荷电聚合物共轭物在电泳过程中的行为。 以这种方式，本发明提供了利用基于大小（包括例如DNA分离和测序技术）分离聚合物分子的这种“最终效应”的方法。

    Branched polymer lables as drag-tags in free solution electrophoresis
    4.
    发明申请
    Branched polymer lables as drag-tags in free solution electrophoresis 审中-公开
    分支聚合物标签作为自由溶液电泳中的拖曳标签

    公开(公告)号：US20070218494A1

    公开(公告)日：2007-09-20

    申请号：US11723321

    申请日：2007-03-19

    申请人： Gary Slater ,  Sorin Nedelcu

    发明人： Gary Slater ,  Sorin Nedelcu

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6869 ,  C12Q2563/149 ,  C12Q2525/161

    摘要： End Labelled Free Solution Electrophoresis (ELFSE) provides a means of separating polymer molecules such as ssDNA according to their size, via free solution electrophoresis, thus eliminating the need for polymer separation via gels or polymer matrices. Here, end labels are provided that optimize branching architecture to increase hydrodynamic drag of the end label, and improve separation of polymer molecules by ELFSE.

    摘要翻译： 结束标记的自由溶液电泳（ELFSE）提供了通过游离溶液电泳来分离其尺寸的聚合物分子例如ssDNA的方法，从而消除了通过凝胶或聚合物基质进行聚合物分离的需要。 在这里，提供终端标签，其优化分支结构以增加终端标签的流体动力学阻力，并通过ELFSE改善聚合物分子的分离。

    Electrophoretic nucleic acid purification method
    5.
    发明授权
    Electrophoretic nucleic acid purification method 失效
    电泳核酸纯化方法

    公开(公告)号：US6146511A

    公开(公告)日：2000-11-14

    申请号：US016531

    申请日：1998-01-30

    申请人： Gary Slater ,  J. William Efcavitch ,  Guy Drouin ,  Pascal Mayer ,  Jean Rousseau ,  Hong Yan Zhou ,  Claudia Chiesa ,  Robert Ruhfel ,  Roger O'Neill

    发明人： Gary Slater ,  J. William Efcavitch ,  Guy Drouin ,  Pascal Mayer ,  Jean Rousseau ,  Hong Yan Zhou ,  Claudia Chiesa ,  Robert Ruhfel ,  Roger O'Neill

    IPC分类号： B01D57/02 ,  B03C5/00 ,  C07H21/02 ,  C07H21/04 ,  C12N15/09 ,  C12N15/10 ,  G01N27/447 ,  G01N27/26

    CPC分类号： C12N15/101 ,  G01N27/447

    摘要： An electrophoretic method for purifying a nucleic acid sample is disclosed. The method generally comprises the steps of (1) providing a nucleic acid sample comprising a desired nucleic acid and one or more contaminants, (2) providing an electrophoresis matrix having a loading well and a recovery well formed therein, (3) placing the nucleic acid sample into the loading well, (4) performing a first electrophoresis comprising electrophoresing the nucleic acid sample for a first time effective to transport the desired nucleic acid out of the loading well and into the electrophoresis matrix; and (5) performing a second electrophoresis comprising electrophoresing the nucleic acid sample for a second time effective to transport the desired nucleic acid out of the electrophoresis matrix and into the recovery well. According to the method, the first and second electrophoresis steps are effective to substantially reduce the concentration of contaminants relative to the concentration of desired nucleic acid in the nucleic acid sample, thereby producing a purified nucleic acid. In the method, the loading and recovery wells may be the same or different, and the electric fields may be DC or alternating. Also disclosed is a preparative electrophoresis method employing an alternating electrical field.

    摘要翻译： 公开了一种用于纯化核酸样品的电泳方法。 该方法通常包括以下步骤：（1）提供包含所需核酸和一种或多种污染物的核酸样品，（2）提供具有载体孔的电泳基质和其中形成的回收孔，（3） （4）进行第一次电泳，其中包括使核酸样品首次有效地将所需核酸转运到载体孔中并进入电泳基质中; 包括进行第二次电泳，其包括将核酸样品电泳第二次，有效将所需核酸转运到电泳基质内并进入回收井。 根据该方法，第一和第二电泳步骤对于相对于核酸样品中所需核酸浓度显着降低污染物的浓度是有效的，从而产生纯化的核酸。 在该方法中，加载和回收井可以相同或不同，并且电场可以是直流或交替的。 还公开了采用交流电场的制备电泳方法。

    Electroosmotic flow for end labelled free solution electrophoresis
    7.
    发明申请
    Electroosmotic flow for end labelled free solution electrophoresis 审中-公开
    用于末端标记自由溶液电泳的电渗流

    公开(公告)号：US20070215472A1

    公开(公告)日：2007-09-20

    申请号：US11724294

    申请日：2007-03-15

    申请人： Gary Slater ,  Laurette McCormick

    发明人： Gary Slater ,  Laurette McCormick

    IPC分类号： C07K1/26 ,  G01N27/00

    CPC分类号： G01N27/44765

    摘要： End Labelled Free Solution Electrophoresis (ELFSE) provides a means of separating polymer molecules such as ssDNA according to their size, via free solution electrophoresis, thus eliminating the need for polymer separation via gels or polymer matrices. Here, significant improvements in ELFSE are disclosed via concurrent exposure of the polymer molecules to an electroosmotic flow. When the methods are applied to DNA sequencing by ELFSE, significant improvements in read length are observed.

    摘要翻译： 结束标记的自由溶液电泳（ELFSE）提供了通过游离溶液电泳来分离其尺寸的聚合物分子例如ssDNA的方法，从而消除了通过凝胶或聚合物基质进行聚合物分离的需要。 在这里，通过将聚合物分子同时暴露于电渗流中，公开了ELFSE的显着改进。 当这些方法应用于ELSEE的DNA测序时，可以观察到阅读长度的显着改善。

    Processes for the preparation and separation of macromolecules
    8.
    发明授权
    Processes for the preparation and separation of macromolecules 失效
    制备和分离大分子的方法

    公开(公告)号：US5348636A

    公开(公告)日：1994-09-20

    申请号：US148239

    申请日：1993-11-08

    申请人： Gary Slater ,  Jaan Noolandi ,  Chantal Turmel

    发明人： Gary Slater ,  Jaan Noolandi ,  Chantal Turmel

    IPC分类号： B01D57/02 ,  C07H1/08 ,  G01N27/447 ,  G01N27/26

    CPC分类号： B01D57/02 ,  G01N27/44773

    摘要： A process for the electrophoretic separation of charged macromolecules includes applying to the macromolecules a periodic sequence of pulses. Each period comprises a multiplicity of electric field pulses of negative and positive polarities. The negative polarity pulses are applied for a longer total time duration than the positive polarity pulses within each period. The average intensity of the negative polarity pulses is less than the average intensity of the positive polarity pulses.

    摘要翻译： 电荷分离电荷大分子的方法包括向大分子施加周期性的脉冲序列。 每个周期包括负极性和正极性的多个电场脉冲。 与每个周期内的正极性脉冲相比，施加负极性脉冲的总持续时间更长。 负极性脉冲的平均强度小于正极性脉冲的平均强度。