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    FET-type biosensor with surface modification
    1.
    发明授权
    FET-type biosensor with surface modification 有权
    具有表面改性的FET型生物传感器

    公开(公告)号：US08735077B2

    公开(公告)日：2014-05-27

    申请号：US11336110

    申请日：2006-01-20

    申请人： Jeo-young Shim ,  Su-hyeon Kim ,  Kyu-tae Yoo ,  Sung-ouk Jung ,  Joon-shik Park

    发明人： Jeo-young Shim ,  Su-hyeon Kim ,  Kyu-tae Yoo ,  Sung-ouk Jung ,  Joon-shik Park

    IPC分类号： G01N33/53

    CPC分类号： B82Y30/00 ,  B82Y15/00 ,  G01N27/4145

    摘要： Provided is a field effect transistor (FET) type biosensor including a source electrode, a gate, and a drain electrode. A ligand that can bind to a side of a nucleic acid is added to the surface of the gate. In a conventional FET type biosensor, it is difficult to detect a signal within the debye length because a target nucleic acid is directly fixed to the surface of a gate of the conventional FET. However, in the present invention, this problem can be overcome and the debye length can be increased by treating the surface of a gate of an FET sensor with a ligand that can bind to a side of a nucleic acid. The ligand can be adsorbed onto the surface of the gate. In this case, the nucleic acid is adsorbed parallel to the surface of the gate, not perpendicular to the surface of the gate, thus generating an effective depletion region. In addition, hybridization efficiency can be increased because a hybridized sample can be injected into an FET sensor at high ionic strength.

    摘要翻译： 提供了包括源电极，栅极和漏电极的场效应晶体管（FET）型生物传感器。 可以结合核酸一侧的配体加入到门的表面。 在传统的FET型生物传感器中，由于目标核酸直接固定在常规FET的栅极表面，难以检测德拜长度内的信号。 然而，在本发明中，可以克服这个问题，并且可以通过用可以结合核酸一侧的配体处理FET传感器的栅极的表面来增加德拜长度。 配体可以吸附在栅极的表面上。 在这种情况下，核酸被平行于栅极的表面吸附，而不垂直于栅极的表面，从而产生有效的耗尽区域。 此外，杂交效率可以提高，因为杂化样品可以以高离子强度注入FET传感器。

    FET-type biosensor with surface modification
    2.
    发明申请
    FET-type biosensor with surface modification 有权
    具有表面改性的FET型生物传感器

    公开(公告)号：US20060205013A1

    公开(公告)日：2006-09-14

    申请号：US11336110

    申请日：2006-01-20

    申请人： Jeo-young Shim ,  Su-hyeon Kim ,  Kyu-tae Yoo ,  Sung-ouk Jung ,  Joon-shik Park

    发明人： Jeo-young Shim ,  Su-hyeon Kim ,  Kyu-tae Yoo ,  Sung-ouk Jung ,  Joon-shik Park

    IPC分类号： G01N33/53

    CPC分类号： B82Y30/00 ,  B82Y15/00 ,  G01N27/4145

    摘要： Provided is a field effect transistor (FET) type biosensor including a source electrode, a gate, and a drain electrode. A ligand that can bind to a side of a nucleic acid is added to the surface of the gate. In a conventional FET type biosensor, it is difficult to detect a signal within the debye length because a target nucleic acid is directly fixed to the surface of a gate of the conventional FET. However, in the present invention, this problem can be overcome and the debye length can be increased by treating the surface of a gate of an FET sensor with a ligand that can bind to a side of a nucleic acid. The ligand can be adsorbed onto the surface of the gate. In this case, the nucleic acid is adsorbed parallel to the surface of the gate, not perpendicular to the surface of the gate, thus generating an effective depletion region. In addition, hybridization efficiency can be increased because a hybridized sample can be injected into an FET sensor at high ionic strength.

    摘要翻译： 提供了包括源电极，栅极和漏电极的场效应晶体管（FET）型生物传感器。 可以结合核酸一侧的配体加入到门的表面。 在传统的FET型生物传感器中，由于目标核酸直接固定在常规FET的栅极表面，难以检测德拜长度内的信号。 然而，在本发明中，可以克服这个问题，并且可以通过用可以结合核酸一侧的配体处理FET传感器的栅极的表面来增加德拜长度。 配体可以吸附在栅极的表面上。 在这种情况下，核酸被平行于栅极的表面吸附，而不垂直于栅极的表面，从而产生有效的耗尽区域。 此外，杂交效率可以提高，因为杂化样品可以以高离子强度注入FET传感器。

    Method for positively detecting toxic material
    4.
    发明授权
    Method for positively detecting toxic material 有权
    积极检测有毒物质的方法

    公开(公告)号：US07981634B2

    公开(公告)日：2011-07-19

    申请号：US11609979

    申请日：2006-12-13

    申请人： Jung-im Han ,  Soo-hyung Choi ,  Soo-suk Lee ,  Sung-ouk Jung ,  Joon-shik Park ,  Ki-eun Kim

    发明人： Jung-im Han ,  Soo-hyung Choi ,  Soo-suk Lee ,  Sung-ouk Jung ,  Joon-shik Park ,  Ki-eun Kim

    IPC分类号： C12Q1/26

    CPC分类号： G01N33/5079 ,  G01N2333/90209

    摘要： Disclosed herein is a method of positively detecting toxic materials within a sample, the method including contacting sub-mitochondrial particles with an electron donor. The electron donor is reacted with an electron transfer system of the sub-mitochondrial particles to produce a reactant. A pH indicator has its pH adjusted to be in a range of 9 to 11. The pH indicator is added to each reactant. Color changes in the pH indicator permit the detection of toxins present in the sample.

    摘要翻译： 本文公开了一种积极检测样品内有毒物质的方法，该方法包括使亚线粒体颗粒与电子给体接触。 电子给体与亚线粒体颗粒的电子转移体系反应以产生反应物。 pH指示剂的pH调节在9至11的范围内。将pH指示剂加入每种反应物中。 pH指示剂中的颜色变化允许检测样品中存在的毒素。

    METHOD AND KIT FOR POSITIVELY DETECTING TOXIC MATERIAL
    5.
    发明申请
    METHOD AND KIT FOR POSITIVELY DETECTING TOXIC MATERIAL 有权
    用于阳极检测毒性材料的方法和工具包

    公开(公告)号：US20070259392A1

    公开(公告)日：2007-11-08

    申请号：US11609979

    申请日：2006-12-13

    申请人： Jung-im Han ,  Soo-hyung Choi ,  Soo-suk Lee ,  Sung-ouk Jung ,  Joon-shik Park ,  Ki-eun Kim

    发明人： Jung-im Han ,  Soo-hyung Choi ,  Soo-suk Lee ,  Sung-ouk Jung ,  Joon-shik Park ,  Ki-eun Kim

    IPC分类号： C12Q1/26 ,  C12Q1/32

    CPC分类号： G01N33/5079 ,  G01N2333/90209

    摘要： A method of positively detecting toxic materials within a sample, the method including contacting sub-mitochondrial particles having competent mitochondrial enzymes formed from inner membranes of mitochondria, an electron donor which transmits electrons to an electron transfer system of the sub-mitochondrial particles and the sample, and forming reaction resultants, adjusting a pH of a pH indicator which change color according to a change in pH, adding the pH indicators to each reaction resultant and identifying color changes of the pH indicator. Also provided is a kit for positively detecting toxic materials within a sample.

    摘要翻译： 一种积极检测样品内的有毒物质的方法，该方法包括使具有由线粒体内膜形成的感受态线粒体酶的亚线粒体颗粒，将电子传递到亚线粒体颗粒的电子转移系统的电子给体和样品 ，并形成反应产物，调节pH指示剂的pH值，根据pH的变化改变颜色，向每个反应产物添加pH指示剂并鉴定pH指示剂的颜色变化。 还提供了用于积极检测样品中有毒物质的试剂盒。

    Kit for positively detecting toxic material
    6.
    发明授权
    Kit for positively detecting toxic material 有权
    用于积极检测有毒物质的试剂盒

    公开(公告)号：US08367367B2

    公开(公告)日：2013-02-05

    申请号：US13155944

    申请日：2011-06-08

    申请人： Jung-im Han ,  Soo-hyung Choi ,  Soo-suk Lee ,  Sung-ouk Jung ,  Joon-shik Park ,  Ki-eun Kim

    发明人： Jung-im Han ,  Soo-hyung Choi ,  Soo-suk Lee ,  Sung-ouk Jung ,  Joon-shik Park ,  Ki-eun Kim

    IPC分类号： C12Q1/26

    CPC分类号： G01N33/5079 ,  G01N2333/90209

    摘要： A method of positively detecting toxic materials within a sample, the method including contacting sub-mitochondrial particles having competent mitochondrial enzymes formed from inner membranes of mitochondria, an electron donor which transmits electrons to an electron transfer system of the sub-mitochondrial particles and the sample, and forming reaction resultants, adjusting a pH of a pH indicator which change color according to a change in pH, adding the pH indicators to each reaction resultant and identifying color changes of the pH indicator. Also provided is a kit for positively detecting toxic materials within a sample.

    摘要翻译： 一种积极检测样品内的有毒物质的方法，该方法包括使具有由线粒体内膜形成的感受态线粒体酶的亚线粒体颗粒，将电子传递到亚线粒体颗粒的电子转移系统的电子给体和样品 ，并形成反应产物，调节pH指示剂的pH值，根据pH的变化改变颜色，向每个反应产物添加pH指示剂并鉴定pH指示剂的颜色变化。 还提供了用于积极检测样品中有毒物质的试剂盒。

    Method of increasing discrimination for single base mismatch using hurdle DNA and artificially mismatched probe
    8.
    发明申请
    Method of increasing discrimination for single base mismatch using hurdle DNA and artificially mismatched probe 有权
    使用障碍DNA和人为错配探针增加单碱基错配的鉴别方法

    公开(公告)号：US20060183142A1

    公开(公告)日：2006-08-17

    申请号：US11335229

    申请日：2006-01-19

    申请人： Soo-hyung Choi ,  Jang-seok Ma ,  Joon-shik Park

    发明人： Soo-hyung Choi ,  Jang-seok Ma ,  Joon-shik Park

    IPC分类号： C12Q1/68 ,  C12P19/34

    CPC分类号： C12Q1/6827 ,  C12Q2537/113 ,  C12Q2527/107 ,  C12Q2525/185

    摘要： A method of increasing discrimination for a target DNA having a polymorphic site is provided. The method includes: synthesizing a first probe by artificially substituting a base of a probe which is fully complementary to a target DNA having a polymorphic site by a mismatched natural base and synthesizing a second probe by artificially substituting a base of another probe having a single base mismatch at a position corresponding to the polymorphic site of the target DNA with a mismatched natural base; synthesizing a first hurdle DNA which is fully complementary to the first probe and is shorter than the first probe and a second hurdle DNA which is fully complementary to the second probe and is shorter than the second probe; immobilizing the first and second probes on a substrate; hybridizing the immobilized first and second probes with the first and second hurdle DNAs, respectively; and hybridizing the target DNA with the hybrids. The addition of a hurdle DNA and variation of a probe base can improve an ability of discriminating a single base mismatch.

    摘要翻译： 提供了增加具有多态位点的靶DNA的鉴别方法。 该方法包括：通过人造取代与具有多态性位点的目标DNA完全互补的基因的错配天然碱合成第一探针，并通过人造地取代具有单个碱基的另一种探针的碱合成第二探针 在对应于具有错配天然碱基的目标DNA的多态性位点的位置处的错配; 合成与第一探针完全互补并且比第一探针短的第一障碍DNA和与第二探针完全互补并且短于第二探针的第二障碍DNA; 将第一和第二探针固定在基底上; 将固定的第一和第二探针分别与第一和第二障碍DNA杂交; 并将靶DNA与杂交体杂交。 障碍DNA的添加和探针碱基的变异可以提高鉴别单一碱基不匹配的能力。

    Method of hybridizing genes
    9.
    发明申请
    Method of hybridizing genes 审中-公开
    杂交基因的方法

    公开(公告)号：US20060204985A1

    公开(公告)日：2006-09-14

    申请号：US11345018

    申请日：2006-02-01

    申请人： Joon-shik Park ,  Myo-yong Lee

    发明人： Joon-shik Park ,  Myo-yong Lee

    IPC分类号： C12Q1/68 ,  C07H21/02 ,  C07H21/04

    CPC分类号： C12Q1/6837 ,  C12Q2527/125

    摘要： Provided are a method of hybridizing genes, including applying to a gene microarray a hybridization solution in which a compound represented by formula (1) is added: where each of R1, R2, and R3 is independently a straight or branched C1-C5 alkyl; and X−is an anion of an organic acid; and a method of using a compound represented by formula (1) as an additive to a hybridization solution.

    摘要翻译： 提供了将基因杂交的方法，包括向基因芯片应用添加了式（1）所示的化合物的杂交溶液：其中R 1，R 2， 而且R 3独立地为直链或支链C 1 -C 5烷基; 且X - 是有机酸的阴离子; 以及使用由式（1）表示的化合物作为杂交溶液的添加剂的方法。