公开(公告)号：US06555813B1

公开(公告)日：2003-04-29

申请号：US09561604

申请日：2000-04-27

申请人： Jody Beecher ,  Frank Scheufele ,  Kamen Voivodov ,  Scot Weinberger ,  William Landgraf

发明人： Jody Beecher ,  Frank Scheufele ,  Kamen Voivodov ,  Scot Weinberger ,  William Landgraf

IPC分类号： G01N2175

CPC分类号： H01J49/0418 ,  B01L3/5085 ,  Y10S977/881 ,  Y10S977/891 ,  Y10S977/892 ,  Y10T436/255

摘要： This invention provides a mass spectrometry probe including a substrate having a surface and a film that coats the surface. The film includes openings that define features for the presentation of an analyte. The film also has a lower surface tension that the surface tension of the substrate surface, and has a water contact angle between 120° and 180°.

摘要翻译： 本发明提供了一种质谱探针，其包括具有表面的基材和涂覆该表面的膜。 该膜包括限定用于呈现分析物的特征的开口。 该膜还具有较低的表面张力，即基材表面的表面张力，并具有120°和180°之间的水接触角。

公开(公告)号：US20100291700A1

公开(公告)日：2010-11-18

申请号：US12799689

申请日：2010-04-28

申请人： Scot Weinberger ,  Stephen Dotson ,  Nathan Harris

发明人： Scot Weinberger ,  Stephen Dotson ,  Nathan Harris

IPC分类号： G01N21/00 ,  G01N30/96

CPC分类号： G01N33/54393 ,  G01N21/45 ,  G01N33/5432 ,  G01N33/54373 ,  G01N33/92

摘要： Devices and methods for detecting interaction between components associated with lipid membranes and analytes are described herein. In certain methods, a surface of a compartment of a device is coated with a material that attaches to lipid membrane from a sample. An analyte is introduced and interaction is detected, for example, by back scattering interferometry. In another method, a surface is passivated with a material that does not bind a lipid membrane. A sample is introduced that contains a membrane comprising a component for testing and an analyte. Interaction is detected. In a third method, a channel is filled with a first liquid that does not comprise a lipid membrane. A bolus of a second liquid that comprises a membrane comprising a component for testing and an analyte is introduced under laminar flow conditions so that a leading edge of the second liquid does not completely displace the first liquid in a sensing area that is interrogated by optical methods, for example, a laser. Interaction between the analyte and the component is detected.

摘要翻译： 本文描述了用于检测与脂质膜和分析物相关的组分之间相互作用的装置和方法。 在某些方法中，装置的隔室的表面涂覆有从样品附着到脂质膜的材料。 引入分析物并检测相互作用，例如通过反向散射干涉测量。 在另一种方法中，用不结合脂质膜的材料钝化表面。 引入了包含含有用于测试的组分和分析物的膜的样品。 检测到相互作用。 在第三种方法中，通道填充有不包含脂质膜的第一液体。 在层流条件下引入包括包含用于测试的组分的膜和分析物的第二液体的推注，使得第二液体的前缘不会在通过光学方法询问的感测区域中完全置换第一液体 ，例如，激光。 检测分析物与组分之间的相互作用。

公开(公告)号：US20100184056A1

公开(公告)日：2010-07-22

申请号：US12655899

申请日：2010-01-08

申请人： Scot Weinberger ,  Darryl J. Bornhop ,  Stephen Dotson ,  Bruce J. Richardson ,  Daniel Chang

发明人： Scot Weinberger ,  Darryl J. Bornhop ,  Stephen Dotson ,  Bruce J. Richardson ,  Daniel Chang

IPC分类号： C12Q1/68 ,  G01N33/53 ,  C12M3/00

CPC分类号： G01N21/45 ,  B01L3/508 ,  B01L2300/0838 ,  G01N2021/4709

摘要： This invention provides a device and method for collection and analysis of heterogeneous samples in a single sample container by back scattering interferometry. The sample container is configured to allow collection of a heterogeneous sample, such as blood, from a subject, separation of insoluble materials, such as blood cells by, for example, centrifugation, and mounting on a back scattering interferometer for analysis. In certain embodiments the container is a capillary tube and the interferometer comprises a mounting to hold the capillary tube in position for analysis. The device and method allow point-of-care analysis of samples.

摘要翻译： 本发明提供了一种用于通过反向散射干涉测量在单个样品容器中收集和分析异质样品的装置和方法。 样品容器被配置为允许从受试者收集诸如血液的异质样品，通过例如离心分离不溶性物质例如血细胞，并将其安装在背散射干涉仪上用于分析。 在某些实施例中，容器是毛细管，并且干涉仪包括用于将毛细管保持在适当位置用于分析的安装件。 该设备和方法允许对样品进行点对点分析。

公开(公告)号：US08450118B2

公开(公告)日：2013-05-28

申请号：US12799689

申请日：2010-04-28

申请人： Scot Weinberger ,  Stephen Dotson ,  Nathan Harris

发明人： Scot Weinberger ,  Stephen Dotson ,  Nathan Harris

IPC分类号： G01N33/00 ,  G01N21/75

CPC分类号： G01N33/54393 ,  G01N21/45 ,  G01N33/5432 ,  G01N33/54373 ,  G01N33/92

摘要： Devices and methods for detecting interaction between components associated with lipid membranes and analytes are described herein. In certain methods, a surface of a compartment of a device is coated with a material that attaches to lipid membrane from a sample. An analyte is introduced and interaction is detected, for example, by back scattering interferometry. In another method, a surface is passivated with a material that does not bind a lipid membrane. A sample is introduced that contains a membrane comprising a component for testing and an analyte. Interaction is detected. In a third method, a channel is filled with a first liquid that does not comprise a lipid membrane. A bolus of a second liquid that comprises a membrane comprising a component for testing and an analyte is introduced under laminar flow conditions so that a leading edge of the second liquid does not completely displace the first liquid in a sensing area that is interrogated by optical methods, for example, a laser. Interaction between the analyte and the component is detected.

摘要翻译： 本文描述了用于检测与脂质膜和分析物相关的组分之间相互作用的装置和方法。 在某些方法中，装置的隔室的表面涂覆有从样品附着到脂质膜的材料。 引入分析物并检测相互作用，例如通过反向散射干涉测量。 在另一种方法中，用不结合脂质膜的材料钝化表面。 引入了包含含有用于测试的组分和分析物的膜的样品。 检测到相互作用。 在第三种方法中，通道填充有不包含脂质膜的第一液体。 在层流条件下引入包括包含用于测试的组分的膜和分析物的第二液体的推注，使得第二液体的前缘不会在通过光学方法询问的感测区域中完全置换第一液体 ，例如，激光。 检测分析物与组分之间的相互作用。

公开(公告)号：US20050112650A1

公开(公告)日：2005-05-26

申请号：US10965092

申请日：2004-10-14

申请人： Daniel Chang ,  Scot Weinberger

发明人： Daniel Chang ,  Scot Weinberger

IPC分类号： B01D20060101 ,  C08G18/08 ,  C08G18/28 ,  C08G18/32 ,  C08G18/38 ,  C08G18/64 ,  C08G18/67 ,  C12M1/34 ,  C12Q1/68 ,  G01N24/00 ,  G01N33/543 ,  G01N33/545

CPC分类号： C08G18/285 ,  C08G18/0814 ,  C08G18/0823 ,  C08G18/0828 ,  C08G18/2825 ,  C08G18/2845 ,  C08G18/3231 ,  C08G18/3872 ,  C08G18/6484 ,  C08G18/6755 ,  C08G2210/00 ,  G01N33/54353 ,  G01N33/545

摘要： Polyurethane polymers bearing multiple reactive groups are readily prepared from easily accessible precursors. The reactive groups of the polymers are then derivatized with binding functionalities for analytes, energy absorbing molecules for matrix assisted laser desorption/ionization mass spectrometry, fluorescent moieties and the like. The reactive groups can also be converted to different reactive groups having a desired avidity or specificity for a selected reaction partner. The polymers are incorporated into devices of use for the analysis, capture, separation, or purification of an analyte. In an exemplary embodiment, the invention provides a substrate coated with a polymer of the invention, the substrate being adapted for use as a probe for a mass spectrometer.

摘要翻译： 具有多个反应性基团的聚氨酯聚合物可以容易地从容易获得的前体制备。 然后将聚合物的反应性基团用分析物的结合官能团，用于基质辅助激光解吸/电离质谱法的能量吸收分子，荧光部分等衍生化。 反应性基团也可以转化为对所选反应配偶体具有所需亲合力或特异性的不同反应性基团。 将聚合物并入用于分析，捕获，分离或纯化分析物的装置中。 在一个示例性实施方案中，本发明提供涂覆有本发明的聚合物的基材，所述基材适于用作质谱仪的探针。

公开(公告)号：US08502985B2

公开(公告)日：2013-08-06

申请号：US13347550

申请日：2012-01-10

申请人： Scot Weinberger ,  Bruce J. Richardson ,  Darryl L. Bornhop

发明人： Scot Weinberger ,  Bruce J. Richardson ,  Darryl L. Bornhop

IPC分类号： G01B9/02

CPC分类号： B01L7/00 ,  B01L3/5027 ,  B01L2300/0654 ,  B01L2300/1822 ,  G01N21/0332 ,  G01N21/11 ,  G01N21/45 ,  G01N2021/0346

摘要： A microfluidic system includes a chip with a microfluidic channel opening onto a chip inlet and a chip outlet. The channel has a sensing area and fluid delivery area. A fluidic adaptor channel opening onto an adaptor inlet and an adaptor outlet can receive a pipette tip through the adaptor inlet wherein receipt of the pipette tip into the adaptor channel creates a direct fluid path between the pipette tip and the channel and wherein the microfluidic system is configured for sensing in the sensing area by interferometry.

摘要翻译： 微流体系统包括具有开口到芯片入口和芯片出口的微流体通道的芯片。 通道具有感测区域和流体输送区域。 在适配器入口和适配器出口上开口的流体适配器通道可以通过适配器入口接收移液管尖端，其中将移液管尖端接收到适配器通道中在移液管尖端和通道之间产生直接的流体路径，并且其中微流体系统 配置为通过干涉测量在感测区域中感测。

公开(公告)号：US20100099203A1

公开(公告)日：2010-04-22

申请号：US12587112

申请日：2009-09-30

申请人： Daniel Chang ,  Scot Weinberger

发明人： Daniel Chang ,  Scot Weinberger

IPC分类号： G01N33/554 ,  B32B17/06 ,  B32B13/04 ,  B32B9/04 ,  B32B15/00 ,  B32B3/02 ,  C12N5/071 ,  B05D3/00 ,  B32B37/02 ,  G01N33/551 ,  G01N33/552 ,  G01N33/544 ,  G01N33/549

CPC分类号： G01N33/544 ,  G01N33/54393 ,  Y10T428/24612 ,  Y10T428/265 ,  Y10T428/31504

摘要： Compositions and methods of modifying surfaces using hydroxyl terminated PEG are described herein. The surfaces so modified are useful in detection of synthetic and natural product organic molecules, organometallics, biomolecules, particles, or cells.

摘要翻译： 本文描述了使用羟基封端的PEG改性表面的组合物和方法。 如此修饰的表面可用于合成和天然产物有机分子，有机金属，生物分子，颗粒或细胞的检测。