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    Method of producing lys-plasminogen
    2.
    发明授权
    Method of producing lys-plasminogen 失效
    产生溶酶原纤溶酶原的方法

    公开(公告)号：US5371007A

    公开(公告)日：1994-12-06

    申请号：US41332

    申请日：1993-04-01

    申请人： Yendra Linnau ,  Ernst Hetzl

    发明人： Yendra Linnau ,  Ernst Hetzl

    IPC分类号： A61K38/46 ,  A61P7/02 ,  C12N9/68 ,  C12S3/14 ,  C12P21/00 ,  C12N9/00

    CPC分类号： C12N9/6435 ,  C12Y304/21007

    摘要： To produce lys-plasminogen having a specific activity of at least 17.5 caseinolytic units/mg protein and at least 50 mymoles/g protein nitrogen as well as an electrophoretic purity of at least 90%, plasminogen from plasma, a plasminogen-containing fraction or a tissue culture is adsorbed on immobilized lysine for the purpose of purification, is eluted and is recovered from the eluate by a protein precipitating agent. A solution of the thus purified plasminogen is adjusted to a plasmin activity ranging between 0.005 and 0.2 mymoles/ml min relative to chromogenic substrate H-D-valyl-L-leucyl-L-lysine-p-nitroanilide dihydrochloride, is maintained at a temperature of from +1.degree. C. to +20.degree. C. for a period of from 6 to 60 hours in order to provoke an enzymatic-proteolytic conversion of plasminogen into lys-plasminogen, whereupon the enzymatic action is interrupted and lys-plasminogen is isolated.

    摘要翻译： 为了产生具有至少17.5个酪蛋白分解单位/ mg蛋白和至少50 mymoles / g蛋白质氮的比活性以及至少90％的电泳纯度的溶血纤溶酶原，血浆中的纤溶酶原，含纤溶酶原的级分或 组织培养物被吸附在固定的赖氨酸上用于纯化，被洗脱并通过蛋白质沉淀剂从洗脱液中回收。 将如此纯化的纤溶酶原的溶液调节至相对于显色底物HD-缬氨酰-L-亮氨酰-L-赖氨酸 - 对硝基苯胺二盐酸盐在0.005至0.2 mymoles / ml min范围内的纤溶酶活性维持在 +1℃至+ 20℃，时间为6至60小时，以引起纤溶酶原转化为溶酶原纤维蛋白溶酶原的酶 - 蛋白水解转化，由此中断酶促作用并分离裂解纤溶酶原。

    Method of producing LYS-plasminogen
    6.
    发明授权
    Method of producing LYS-plasminogen 失效
    生产LYS-纤溶酶原的方法

    公开(公告)号：US5556766A

    公开(公告)日：1996-09-17

    申请号：US292290

    申请日：1994-08-18

    申请人： Yendra Linnau ,  Ernst Hetzl

    发明人： Yendra Linnau ,  Ernst Hetzl

    IPC分类号： A61K38/46 ,  A61P7/02 ,  C12N9/68 ,  C12S3/14 ,  C12P21/06 ,  C12N9/00

    CPC分类号： C12N9/6435 ,  C12Y304/21007

    摘要： A process for preparing lys-plasminogen from blood plasma or blood plasma products having a specific activity of at least 17.5 caseinolytic units/mg protein and at lease 50 .mu.moles/g protein nitrogen as well as a an electrophoretic purity of at least 90% is presented. Cohn fraction III is obtained by phosphate precipitation from blood plasma or blood plasma products. A crude plasminogen fraction is then obtained by precipitating most non-plasminogen proteins with ethanol. The crude plasminogen is absorbed on immobilized lysine affinity column. Elution followed by precipitation obtains a purified plasminogen. The purified plasminogen is converted to lys-plasminogen with plasmin having an activity from 0.01 to 0.1 .mu.moles/ml min relative to chromogenic substrate H-D-Val-L-Leu-L-Lys-p-nitroanilide by dialyzing at a temperature of about 1.degree. to 20.degree. C. for a time period of about 6 to 60 hours. The conversion is interrupted by adding aprotinin. The resulting lys-plasminogen is lyophilized to produce a lyophilized lys-plasminogen.

    摘要翻译： 从具有至少17.5％酪蛋白溶解单位/ mg蛋白质的比活性和至少50μg/ g蛋白质氮的比活性以及至少90％的电泳纯度的血浆或血浆产物制备lys-纤溶酶原的方法是 呈现。 通过从血浆或血浆产物中的磷酸盐沉淀获得Cohn级分III。 然后通过用乙醇沉淀大多数非纤溶酶原蛋白来获得粗纤溶酶原级分。 粗纤溶酶原被固定化的赖氨酸亲和柱吸收。 洗脱后沉淀得到纯化的纤溶酶原。 通过在约1℃的温度下透析，相对于显色底物HD-Val-L-Leu-L-Lys-对硝基苯胺将纯化的纤溶酶原转化为具有0.01至0.1μmol/ ml min活性的纤溶酶的lys-纤溶酶原 在-20℃下，时间约6〜60小时。 转化通过加入抑肽酶中断。 将所得的溶血纤维蛋白溶酶原冻干，以产生冻干溶胞纤溶酶原。

    Method of inactivating reproductive filterable pathogens
    9.
    发明授权
    Method of inactivating reproductive filterable pathogens 失效
    灭活生殖可过滤病原体的方法

    公开(公告)号：US4814277A

    公开(公告)日：1989-03-21

    申请号：US48774

    申请日：1987-05-12

    申请人： Johann Eibl ,  Yendra Linnau

    发明人： Johann Eibl ,  Yendra Linnau

    IPC分类号： A61K39/395 ,  A61K38/00 ,  C07K16/00 ,  C07K16/06 ,  C12N7/04 ,  C07K3/00

    CPC分类号： C07K16/00 ,  A61K38/00

    摘要： A method of inactivating reproductive filterable pathogens in immunoglobulin-G-containing blood fractions to be applied therapeutically or prophylactically. In order to preserve full activity of the blood products and to completely inactivate pathogenic viruses, an aqueous solution of an immunoglobulin-G-containing fraction obtained from human blood is treated with neutral hydrolases at a temperature of 4.degree. to 50.degree. C. and at a pH of 5.5 to 9.5.

    摘要翻译： 一种使免疫球蛋白G含量血液成分中的生殖可过滤病原体失活的方法，用于治疗或预防性应用。 为了保持血液制品的全部活性并完全灭活病原体病毒，将从人血液获得的含有免疫球蛋白G的级分的水溶液用中性水解酶在4℃至50℃的温度和 pH为5.5至9.5。