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    Method and device for removal of cryoprotectant from cryopreserved biological cells and tissues
    4.
    发明授权
    Method and device for removal of cryoprotectant from cryopreserved biological cells and tissues 失效
    从冷冻保存的生物细胞和组织中去除冷冻保护剂的方法和装置

    公开(公告)号：US06869758B1

    公开(公告)日：2005-03-22

    申请号：US10206539

    申请日：2002-07-26

    申请人： Dayong Gao ,  XiangDong Cui

    发明人： Dayong Gao ,  XiangDong Cui

    IPC分类号： A01N1/02 ,  A01N1/00 ,  C12N5/00

    CPC分类号： A01N1/0278 ,  A01N1/0221 ,  B01D63/02

    摘要： Novel methods and devices for removing cryoprotectant from cryoprotectant-containing liquids, and from cells residing therein, are disclosed. In one aspect, the method comprises passing the cryoprotectant-containing liquid through at least one semipermeable hollow fiber membrane contained in a hollow module in a first direction, while passing a liquid which is substantially free of cryoprotectant through the hollow module in a second direction to remove cryoprotectant across a diffusion gradient. In another aspect, a device is described for removing cryoprotectant from a liquid, comprising a hollow module with at least one semipermeable hollow fiber membrane therein for accomplishing such counter-current diffusion removal of cryprotectant. A software program is also provided for predicting optimal flow rates through the device of the invention, thereby allowing optimal cryoprotectant removal regardless of the cryoprotectant used or the material from which the semipermeable hollow fiber membrane is fabricated.

    摘要翻译： 公开了用于从含有冷冻保护剂的液体以及从其中存在的细胞中除去冷冻保护剂的新方法和装置。 在一个方面，该方法包括使包含冷冻保护剂的液体沿第一方向通过包含在中空模块中的至少一个半透性中空纤维膜，同时使基本上不含冷冻保护剂的液体沿着第二方向穿过中空模块， 通过扩散梯度去除冷冻保护剂。 在另一方面，描述了用于从液体中除去冷冻保护剂的装置，其包括其中具有至少一个半透性中空纤维膜的空心模块，用于实现防流体的这种逆流扩散去除。 还提供了用于预测通过本发明的装置的最佳流速的软件程序，从而允许最佳的冷冻保护剂去除，而不管所用的冷冻保护剂如何，或者制造半透性中空纤维膜的材料。

    General method to quickly add cryoprotectants to animal cells while maintaining viability
    5.
    发明授权
    General method to quickly add cryoprotectants to animal cells while maintaining viability 失效
    一般方法可以快速添加冷冻保护剂到动物细胞，同时保持生存力

    公开(公告)号：US5753427A

    公开(公告)日：1998-05-19

    申请号：US478873

    申请日：1995-06-07

    申请人： John K. Critser ,  Dayong Gao

    发明人： John K. Critser ,  Dayong Gao

    IPC分类号： A01N1/02 ,  A61K35/52

    CPC分类号： A01N1/0263 ,  A01N1/0221 ,  A61K35/52

    摘要： A mathematical model, and membranes and devices based upon that model, to optimize protocols for the addition or removal of cryoprotectant to or from biological cells. This disclosure describes the use of four equations to predict optimal protocols to add or remove cryoprotectant to or from biological cells. The equations particularly require experimentally found data, specific to cell-type and species, regarding the osmotic tolerance of the cells, where osmotic tolerance refers to the cells ability to shrink or swell to various changes in osmolality without injury. The equations further require the cryoprotectant permeability coefficient and the water permeability coefficient of the particular cells' plasma membrane. These coefficients are found with experimental data of the knetic volume change of the cell-type to a known concentration and temperature of cryoprotectant. Also disclosed are particularly preferred methods and devices to add or remove cryoprotectant to or from these cells based upon these equations.

    摘要翻译： 数学模型，以及基于该模型的膜和装置，以优化用于向生物细胞或从生物细胞添加或除去冷冻保护剂的方案。 本公开描述了使用四个方程来预测最终方案以向生物细胞或从生物细胞添加或除去冷冻保护剂。 方程式特别需要关于细胞类型和物种特异性的实验发现的数据，关于细胞的渗透性耐受性，其中渗透性是指细胞在不损伤的情况下收缩或膨胀到各种重量摩尔渗透压变化的能力。 方程进一步要求特定细胞质膜的冷冻保护剂渗透系数和水渗透系数。 发现这些系数与细胞类型的体积变化的实验数据与已知的冷冻保护剂浓度和温度有关。 还公开了基于这些方程特别优选的方法和装置，以向这些细胞添加或除去冷冻保护剂。

    Methods and devices for cryopreservation of biological cells and tissues
    6.
    发明授权
    Methods and devices for cryopreservation of biological cells and tissues 失效
    用于冷冻保存生物细胞和组织的方法和装置

    公开(公告)号：US07604930B1

    公开(公告)日：2009-10-20

    申请号：US11007096

    申请日：2004-12-08

    申请人： Dayong Gao ,  Gary Van Zant ,  XiangDong Cui

    发明人： Dayong Gao ,  Gary Van Zant ,  XiangDong Cui

    IPC分类号： A01N1/02 ,  A01N65/00

    CPC分类号： A01N1/02 ,  A01N1/0221 ,  A01N1/0284

    摘要： Novel methods, compositions, and devices for achieving optimal cooling of living cells during cryopreservation are disclosed. In one aspect, the method comprises gradually cooling the cell to a first predetermined temperature, followed by rapidly cooling the cell to a second predetermined temperature. In another aspect, a device is described for achieving a desired cooling rate for a cell, comprising a first container for holding a cell, a second container for holding the first container, and optionally a frame for holding the first container in a spaced apart relationship with the second container. The method of the invention comprises placing cells into the first container, placing the first container in the second container and sealing the second container, and placing the second container in a suitable cooling device. In yet another aspect, novel cryoprotectant compositions are provided comprising conventional cryoprotectant plus one or more high molecular weight cryoprotectants.

    摘要翻译： 公开了用于在冷冻保存期间实现活细胞的最佳冷却的新方法，组合物和装置。 一方面，该方法包括将电池逐渐冷却至第一预定温度，然后将电池快速冷却至第二预定温度。 在另一方面，描述了一种用于实现电池的期望冷却速率的装置，包括用于容纳电池的第一容器，用于保持第一容器的第二容器和用于将第一容器保持间隔开的关系的框架 与第二个容器。 本发明的方法包括将细胞放入第一容器中，将第一容器放置在第二容器中并密封第二容器，并将第二容器放置在合适的冷却装置中。 在另一方面，提供了包含常规冷冻保护剂加上一种或多种高分子量冷冻保护剂的新型冷冻保护剂组合物。

    Step-wise method to remove cryoprotectant from sperm
    7.
    发明授权
    Step-wise method to remove cryoprotectant from sperm 失效
    从精子中去除冷冻保护剂的逐步方法

    公开(公告)号：US5595866A

    公开(公告)日：1997-01-21

    申请号：US250675

    申请日：1994-05-27

    申请人： John K. Critser ,  Dayong Gao

    发明人： John K. Critser ,  Dayong Gao

    IPC分类号： A01N1/02 ,  A61K35/52

    CPC分类号： A01N1/0263 ,  A01N1/0221 ,  A61K35/52

    摘要： A mathematical model to optimize protocols for the addition or removal of cryoprotectant to or from sperm cells. This disclosure describes the use of four equations to predict optimal protocols to add or remove cryoprotectant to or from sperm cells. The equations particularly require experimentally found data, specific to a species of sperm, regarding the osmotic tolerance of sperm cells, where osmotic tolerance refers to the sperm cells ability to shrink or swell to various changes in osmolality without injury. The equations further require the cryoprotectant permeability coefficient and the water permeability coefficient of the particular sperm cells' plasma membrane. Also disclosed are two particularly preferred methods to add or remove cryoprotectant to or from sperm. These preferred methods are Fixed-Volume-Step addition/removal of cryoprotectant or Fixed-Molarity-Step addition/removal of cryoprotectant.

    摘要翻译： 优化用于向精子细胞加入或除去冷冻保护剂的方案的数学模型。 本公开描述了使用四个方程来预测最佳方案以向精子细胞中加入或除去冷冻保护剂。 方程式特别需要关于精子种类的实验发现的数据，关于精子细胞的渗透容忍度，其中渗透性是指精子细胞在不损伤的情况下收缩或膨胀至各种渗透压变化的能力。 方程进一步要求特定精子细胞质膜的冷冻保护剂渗透系数和水渗透系数。 还公开了向冷冻保护剂添加或除去精子的两种特别优选的方法。 这些优选的方法是固定 - 体积 - 步骤添加/去除冷冻保护剂或固定 - 摩尔级 - 添加/去除冷冻保护剂。

    Cell-type-specific methods and devices for the low temperature preservation of the cells of an animal species
    8.
    发明授权
    Cell-type-specific methods and devices for the low temperature preservation of the cells of an animal species 失效
    用于动物物种细胞的低温保存的细胞型特异性方法和装置

    公开(公告)号：US06054287A

    公开(公告)日：2000-04-25

    申请号：US32071

    申请日：1998-02-27

    申请人： Dayong Gao ,  John K. Critser

    发明人： Dayong Gao ,  John K. Critser

    IPC分类号： A01N1/02 ,  C12Q1/02

    CPC分类号： A01N1/0284 ,  A01N1/0221 ,  A01N1/0278

    摘要： A mathematical model, the membranes and devices based upon that model to optimize protocols for the addition or removal of cryoprotectant to or from biological cells, and a method to observe the biological cells and obtain the data to implement the models. This disclosure describes the use of four equations to predict optimal protocols to add or remove cryoprotectant to or from biological cells. The equations particularly require experimentally found data, specific to cell-type and species, regarding the osmotic tolerance of the cells, where osmotic tolerance refers to the cells ability to shrink or swell to various changes in osmolality without injury. The equations further require the cryoprotectant permeability coefficient and the water permeability coefficient of the particular cells' plasma membrane. These coefficients are found with experimental data of the knetic volume change of the cell-type to a known concentration and temperature of cryoprotectant, and one method is particularly presented as to how this data may be obtained. Also disclosed are particularly preferred methods and devices to add or remove cryoprotectant to or from these cells based upon these equations.

    摘要翻译： 数学模型，基于该模型的膜和装置优化用于向生物细胞或从生物细胞添加或除去冷冻保护剂的方案，以及观察生物细胞并获得数据以实施模型的方法。 本公开描述了使用四个方程来预测最终方案以向生物细胞或从生物细胞添加或除去冷冻保护剂。 方程式特别需要关于细胞类型和物种特异性的实验发现的数据，关于细胞的渗透性耐受性，其中渗透性是指细胞在不损伤的情况下收缩或膨胀到各种重量摩尔渗透压变化的能力。 方程进一步要求特定细胞质膜的冷冻保护剂渗透系数和水渗透系数。 发现这些系数与细胞类型的体积变化与冷冻保护剂的已知浓度和温度的实验数据相关，并且特别提出了如何获得该数据的一种方法。 还公开了基于这些方程特别优选的方法和装置，以向这些细胞添加或除去冷冻保护剂。

    Method and device for gastric line insertion
    9.
    发明授权
    Method and device for gastric line insertion 失效
    胃线插入方法和装置

    公开(公告)号：US5611787A

    公开(公告)日：1997-03-18

    申请号：US322579

    申请日：1994-10-13

    申请人： Robert J. Demeter ,  Henry C. Bock ,  Dayong Gao

    发明人： Robert J. Demeter ,  Henry C. Bock ,  Dayong Gao

    IPC分类号： A61J15/00 ,  A61M25/01 ,  A61M31/00

    CPC分类号： A61J15/003 ,  A61J15/0007 ,  A61M25/01 ,  A61J15/0061 ,  A61M2025/0183

    摘要： The present invention provides a method and device for placement of a gastric line in the digestive tract of a patient. A guide element is either formed integrally with the end of a gastric line or it is formed separately to be fixed on the end segment of a gastric line to reduce the flexibility of the gastric line segment and facilitate the positioning of the gastric line in a patient's digestive tract. The guide element is provided with means for slideably engaging a previously positioned transepiglottal guide line so that the guide element when engaged with the guide line, can be pushed down the guide line to position the gastric line in an esophageal or post-esophageal position. An optional mouthpiece is provided to receive and guide the gastric tube toward the middle of the posterior oral pharynx during insertion and to protect the gastric line from the biting surfaces of the teeth and maintain its position in the mouth after insertion.

    摘要翻译： 本发明提供了一种在患者的消化道中放置胃肠道的方法和装置。 引导元件或者与胃线的一端形成一体，或者单独形成以固定在胃线的末端部分上，以降低胃线段的柔韧性，并且便于将胃线定位在病人体内 消化道。 引导元件设置有用于可滑动地接合先前定位的经视线引导线的装置，使得当引导元件与引导线接合时可以将引导元件向下推到引导线以将胃线定位在食管或食管后位置。 提供可选的接口，用于在插入期间将胃管朝向后咽口的中部接收和引导，并且保护胃线免受牙齿咬合表面的影响，并在插入后保持其在口中的位置。