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    Measuring with zero volume cell
    1.
    发明授权
    Measuring with zero volume cell 失效
    用零容量单元进行测量

    公开(公告)号：US4911794A

    公开(公告)日：1990-03-27

    申请号：US65418

    申请日：1987-06-18

    申请人： John W. Parce ,  Robert F. Zuk

    发明人： John W. Parce ,  Robert F. Zuk

    IPC分类号： C12Q1/00 ,  G01N27/28 ,  G01N27/30 ,  G01N27/414 ,  G01N27/416

    CPC分类号： G01N27/3271 ,  Y10S435/817 ,  Y10S436/806

    摘要： Methods and apparatuses are described for determining the presence of an analyte in a sample suspected of containing the analyte. The method comprises contacting a concentrate of a determinable element capable of being detected by means of a semiconductive electrode with the electrode in the presence of a relatively large volume of an assay medium. The determinable element is present in the concentrate in the amount related to the amount of analyte present in the sample. The volume of the medium in diffusive communication with the concentrate is then reduced and the determinable element is detected by means of the effect that the determinable element has on the semiconductive electrode.

    摘要翻译： 描述了用于确定疑似含有分析物的样品中分析物的存在的方法和装置。 该方法包括在相对较大体积的测定培养基的存在下使能够通过半导体电极检测的可测定元件的浓缩物与电极接触。 浓缩物中可测量的元素以与样品中存在的分析物的量相关的量存在。 然后减少与浓缩物扩散连通的介质的体积，并且通过可测定元件在半导体电极上具有的作用来检测可确定元件。

    Zero volume electrochemical cell
    2.
    发明授权
    Zero volume electrochemical cell 失效
    零容电化学电池

    公开(公告)号：US5395503A

    公开(公告)日：1995-03-07

    申请号：US129541

    申请日：1993-09-29

    申请人： John W. Parce ,  Robert F. Zuk

    发明人： John W. Parce ,  Robert F. Zuk

    IPC分类号： G01N27/28 ,  G01N27/416 ,  G01N27/327

    CPC分类号： G01N27/327

    摘要： Methods and apparatuses are described for determining the presence of an analyte in a sample suspected of containing the analyte. The method comprises contacting a concentrate of a determinable element capable of being detected by means of a semiconductive electrode with the electrode in the presence of a relatively large volume of an assay medium. The determinable element is present in the concentrate in the amount related to the amount of analyte present in the sample. The volume of the medium in diffusive communication with the concentrate is then reduced and the determinable element is detected by means of the effect that the determinable element has on the semiconductive electrode.

    摘要翻译： 描述了用于确定疑似含有分析物的样品中分析物的存在的方法和装置。 该方法包括在相对较大体积的测定培养基的存在下使能够通过半导体电极检测的可测定元件的浓缩物与电极接触。 浓缩物中可测量的元素以与样品中存在的分析物的量相关的量存在。 然后减少与浓缩物扩散连通的介质的体积，并且通过可测定元件在半导体电极上具有的作用来检测可确定元件。

    Zero volume cell
    3.
    发明授权
    Zero volume cell 失效
    零体积单元

    公开(公告)号：US4915812A

    公开(公告)日：1990-04-10

    申请号：US876925

    申请日：1986-06-20

    申请人： John W. Parce ,  Robert F. Zuk

    发明人： John W. Parce ,  Robert F. Zuk

    IPC分类号： G01N27/28 ,  G01N27/416

    CPC分类号： G01N27/327

    摘要： Methods and apparatuses are described for determining the presence of an analyte in a sample suspected of containing the analyte. The method comprises contacting a concentrate of a determinable element capable of being detected by means of a semiconductive electrode with the electrode in the presence of a relatively large volume of an assay medium. The determinable element is present in the concentrate in the amount related to the amount of analyte present in the sample. The volume of the medium in diffusive communication with the concentrate is then reduced and the determinable element is detected by means of the effect that the determinable element has on the semiconductive electrode.

    摘要翻译： 描述了用于确定疑似含有分析物的样品中分析物的存在的方法和装置。 该方法包括在相对较大体积的测定培养基的存在下使能够通过半导体电极检测的可测定元件的浓缩物与电极接触。 浓缩物中可测量的元素以与样品中存在的分析物的量相关的量存在。 然后减少与浓缩物扩散连通的介质的体积，并且通过可测定元件在半导体电极上具有的作用来检测可确定元件。

    DETECTION SYSTEM AND METHOD FOR HIGH SENSITIVITY FLUORESCENT ASSAYS
    5.
    发明申请
    DETECTION SYSTEM AND METHOD FOR HIGH SENSITIVITY FLUORESCENT ASSAYS 有权
    检测系统和高灵敏度荧光测定方法

    公开(公告)号：US20110312105A1

    公开(公告)日：2011-12-22

    申请号：US13224174

    申请日：2011-09-01

    申请人： Hong Tan ,  Robert F. Zuk ,  Yushan Tan ,  Erhua Cao ,  Min Xia ,  Jun Chen

    发明人： Hong Tan ,  Robert F. Zuk ,  Yushan Tan ,  Erhua Cao ,  Min Xia ,  Jun Chen

    IPC分类号： G01N21/64 ,  C07K14/36 ,  C07K16/00

    CPC分类号： G01N33/54366 ,  C08B37/006 ,  G01N21/6428 ,  G01N21/645 ,  G01N33/54373 ,  G01N2021/6439 ,  G01N2201/06113

    摘要： This invention relates to a detection system for measuring a fluorescent signal in a fluorescent assay. The system comprises a probe having a small sensing surface bound with a fluorescent label, and a light source and a detector both mounted at the proximal side of the sensing surface of the substrate. The invention also relates to a method for detecting an analyte in a liquid sample using a probe tip having a small surface area (≦5 mm) and a high molecular weight polymer (≧1 MD) having multiple binding molecules and multiple fluorescent labels. The binding reaction is accelerated by flowing the reaction solutions laterally and moving the probe tip up and down in the reaction vessels. The invention furthers relates to a fluorescent labeling composition comprising a cross-linked Ficoll molecule having a plurality of binding molecules and a plurality of fluorescent labels.

    摘要翻译： 本发明涉及一种用于在荧光测定中测量荧光信号的检测系统。 该系统包括具有与荧光标签结合的小感测表面的探针，以及安装在基底的感测表面的近侧的光源和检测器。 本发明还涉及一种使用具有小表面积（＆NlE; 5mm）的探针尖端和具有多个结合分子和多个荧光标记的高分子量聚合物（≥1MD）来检测液体样品中的分析物的方法。 通过横向流动反应溶液并在反应容器中上下移动探针尖端来加速结合反应。 本发明还涉及包含具有多个结合分子和多个荧光标记的交联Ficoll分子的荧光标记组合物。

    Methods and kits for the inhibition of hyperplasia in vascular fistulas and grafts
    6.
    发明授权
    Methods and kits for the inhibition of hyperplasia in vascular fistulas and grafts 失效
    用于抑制血管瘘和移植物增生的方法和试剂盒

    公开(公告)号：US06755853B2

    公开(公告)日：2004-06-29

    申请号：US10010411

    申请日：2001-12-05

    申请人： John R. McKenzie ,  Robert F. Zuk ,  Axel F. Brisken ,  Paul D. Corl

    发明人： John R. McKenzie ,  Robert F. Zuk ,  Axel F. Brisken ,  Paul D. Corl

    IPC分类号： A61F206

    CPC分类号： A61F2/02 ,  A61F2/06

    摘要： A method for inhibiting hyperplasia at an anastomotic junction of a vascular fistula or vascular graft comprises exposing the anastomotic junction to vibrational energy at a mechanical index and for a time sufficient to inhibit hyperplasia. Kits are described comprising the catheter or a probe suitable for performing the methods together with instructions for use setting forth the methods. The methods and kits are particularly suitable for treating anastomotic junctions formed to provide vascular access for hemodialysis, hemofiltration, and the like. The methods and kits are also suitable for treating other vascular grafts, such as those formed during cardiac bypass graft surgery, vascular repair, and the like.

    摘要翻译： 用于抑制血管瘘或血管移植物的吻合接合处的增生的方法包括以机械指数将吻合接头暴露于振动能量并足以抑制增生的时间。 描述的试剂盒包括适于执行方法的导管或探针以及阐述方法的使用说明书。 所述方法和试剂盒特别适用于治疗形成的吻合口以提供用于血液透析，血液滤过等的血管通路。 方法和试剂盒也适用于治疗其它血管移植物，例如在心脏旁路移植手术期间形成的血管移植物，血管修复等。

    Method and kit for pyridinium crosslink assay
    8.
    发明授权
    Method and kit for pyridinium crosslink assay 失效
    吡啶交联试验方法和试剂盒

    公开(公告)号：US5620861A

    公开(公告)日：1997-04-15

    申请号：US484212

    申请日：1995-06-07

    申请人： Mary J. Cerelli ,  Hsin-Shan J. Ju ,  Robert F. Zuk

    发明人： Mary J. Cerelli ,  Hsin-Shan J. Ju ,  Robert F. Zuk

    IPC分类号： A61C19/06 ,  C07K16/18 ,  G01N33/53 ,  G01N33/574 ,  G01N33/68 ,  G01N33/543

    CPC分类号： C07K16/18 ,  G01N33/5308 ,  G01N33/57407 ,  G01N33/6887 ,  A61C19/063 ,  G01N2800/108 ,  Y10S435/81 ,  Y10S435/975

    摘要： A method of assaying bone collagen degradation activity in a human subject. In the method, a human urine sample is reacted with an antibody which (i) is capable of reacting immunospecifically with pyridinium crosslinks selected from the group consisting of native free pyridinoline and native free deoxypyridinoline, and (ii) has a ratio of reactivity toward the selected pyridinium crosslinks and urinary pyridinium peptides larger than 1,000 daltons in molecular weight, of greater than about 5:1. An immunocomplex forms between the antibody reagent and the selected pyridinium crosslinks which are present in the sample, and the amount of immunocomplex is measured. Also disclosed are antibody reagents and kits which can be used in the method.

    摘要翻译： 测定人类受试者骨胶原降解活性的方法。 在该方法中，人尿样品与抗体反应，（i）能够与选自天然游离吡啶啉和天然游离的脱氧吡啶啉的吡啶鎓交联物进行免疫特异性反应，和（ii）具有与 选择的吡啶鎓交联剂和大于1000道尔顿的尿吡啶肽分子量大于约5：1。 在抗体试剂和存在于样品中的所选择的吡啶鎓交联之间形成免疫复合物，并测量免疫复合物的量。 还公开了可用于该方法的抗体试剂和试剂盒。

    Heterogeneous assay having delayed signal production
    9.
    发明授权
    Heterogeneous assay having delayed signal production 失效
    具有延迟信号产生的异构测定

    公开(公告)号：US5089383A

    公开(公告)日：1992-02-18

    申请号：US278686

    申请日：1988-12-01

    申请人： Sydney Leeder ,  Robert F. Zuk

    发明人： Sydney Leeder ,  Robert F. Zuk

    IPC分类号： B01D15/08 ,  C12Q1/00 ,  G01N33/542 ,  G01N33/543

    CPC分类号： G01N33/542 ,  G01N33/54306 ,  Y10S435/805 ,  Y10S435/97 ,  Y10S436/81

    摘要： Methods and compositions are provided for assays involving members of a specific binding pair ("sbp members") and members of a signal producing system ("sps members"). The signal producing system is capable of producing a detectible signal in relation to the presence or amount of an analyte in a sample suspected of containing the analyte. Exemplary of sps members are enzymes and enzyme substrates, which react with each other to produce a signal. The improvement of the present invention comprises temporarily delaying the production of the signal without subsequent reagent addition. The delay can be achieved by employing an inhibitor which can be an alternate substrate for the enzyme or a compound which reacts with the product of the enzyme and its substrate in an effective amount.

    摘要翻译： 提供了涉及特异性结合对（“sbp成员”）成员和信号产生系统（“sps成员”）的成员的测定的方法和组合物。 信号产生系统能够产生与怀疑含有分析物的样品中分析物的存在或量相关的可检测信号。 sps成员的示例是酶和酶底物，其彼此反应以产生信号。 本发明的改进包括临时延迟信号的产生，而不需要随后的试剂添加。 延迟可以通过使用可以作为酶的替代底物的抑制剂或与酶和其底物的产物有效量反应的化合物来实现。