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    ELECTROCHEMICAL BIOSENSOR
    1.
    发明申请
    ELECTROCHEMICAL BIOSENSOR 有权
    电化学生物传感器

    公开(公告)号：US20100285514A1

    公开(公告)日：2010-11-11

    申请号：US12694876

    申请日：2010-01-27

    申请人： Jonathan Clay Claussen ,  Aaron D. Franklin ,  Timothy S. Fisher ,  D. Marshall Porterfield

    发明人： Jonathan Clay Claussen ,  Aaron D. Franklin ,  Timothy S. Fisher ,  D. Marshall Porterfield

    IPC分类号： C12Q1/26 ,  C12M1/34 ,  C23C28/00

    CPC分类号： C12Q1/006 ,  C12Q1/26 ,  C23C28/322 ,  C23C28/345 ,  G01N27/3271

    摘要： Networks of single-walled carbon nanotubes (SWCNTs) decorated with Au-coated Pd (Au/Pd) nanocubes are employed as electrochemical biosensors that exhibit excellent sensitivity (2.6 mA mM−1 cm−2) and a low estimated detection limit (2.3 nM) at a signal-to-noise ratio of 3 (S/N=3) in the amperometric sensing of hydrogen peroxide. Biofunctionalization of the Au/Pd nanocube-SWCNT biosensor is demonstrated with the selective immobilization of fluorescently labeled streptavidin on the nanocube surfaces via thiol linking. Similarly, glucose oxidase (GOx) is linked to the surface of the nanocubes for amperometric glucose sensing. The exhibited glucose detection limit of 1.3_M (S/N=3) and linear range spanning from 10 μM to 50 mM substantially surpass other CNT-based biosensors. These results, combined with the structure's compatibility with a wide range of biofunctionalization procedures, would make the nanocube-SWCNT biosensor exceptionally useful for glucose detection in diabetic patients and well suited for a wide range of amperometric detection schemes for biomarkers.

    摘要翻译： 用Au涂覆的Pd（Au / Pd）纳米管装饰的单壁碳纳米管（SWCNT）的网络被用作显示出优异的灵敏度（2.6mAm-1cm-2）和低估计检测限（2.3nM）的电化学生物传感器 ）在过氧化氢的电流检测中的信噪比为3（S / N = 3）。 Au / Pd纳米管SWCNT生物传感器的生物功能化通过硫醇连接在荧光标记的链霉抗生物素蛋白选择性固定在纳米管表面上得到证明。 类似地，葡萄糖氧化酶（GOx）连接到纳米管的表面用于电流葡萄糖感测。 显示的葡萄糖检测限为1.3_M（S / N = 3）和跨越10μM至50 mM的线性范围基本上超过其他基于CNT的生物传感器。 这些结果结合结构与广泛的生物功能化程序的相容性将使得纳米管SWCNT生物传感器在糖尿病患者中非常适用于葡萄糖检测，非常适用于生物标志物的广泛电流检测方案。

    Electrochemical biosensor
    2.
    发明授权
    Electrochemical biosensor 有权
    电化学生物传感器

    公开(公告)号：US08715981B2

    公开(公告)日：2014-05-06

    申请号：US12694876

    申请日：2010-01-27

    申请人： Jonathan Clay Claussen ,  Aaron D. Franklin ,  Timothy S. Fisher ,  D. Marshall Porterfield

    发明人： Jonathan Clay Claussen ,  Aaron D. Franklin ,  Timothy S. Fisher ,  D. Marshall Porterfield

    IPC分类号： C12N11/00 ,  C12N11/14 ,  C12N9/02 ,  C12N9/00 ,  C12N9/04 ,  C12N13/00 ,  C12M1/00 ,  C25B11/00 ,  C12Q1/26

    CPC分类号： C12Q1/006 ,  C12Q1/26 ,  C23C28/322 ,  C23C28/345 ,  G01N27/3271

    摘要： Networks of single-walled carbon nanotubes (SWCNTs) decorated with Au-coated Pd (Au/Pd) nanocubes are employed as electrochemical biosensors that exhibit excellent sensitivity (2.6 mA mM−1 cm−2) and a low estimated detection limit (2.3 nM) at a signal-to-noise ratio of 3 (S/N=3) in the amperometric sensing of hydrogen peroxide. Biofunctionalization of the Au/Pd nanocube-SWCNT biosensor is demonstrated with the selective immobilization of fluorescently labeled streptavidin on the nanocube surfaces via thiol linking. Similarly, glucose oxidase (GOx) is linked to the surface of the nanocubes for amperometric glucose sensing. The exhibited glucose detection limit of 1.3_M (S/N=3) and linear range spanning from 10 μM to 50 mM substantially surpass other CNT-based biosensors. These results, combined with the structure's compatibility with a wide range of biofunctionalization procedures, would make the nanocube-SWCNT biosensor exceptionally useful for glucose detection in diabetic patients and well suited for a wide range of amperometric detection schemes for biomarkers.

    摘要翻译： 用Au涂覆的Pd（Au / Pd）纳米管装饰的单壁碳纳米管（SWCNT）的网络被用作显示出优异的灵敏度（2.6mAm-1cm-2）和低估计检测限（2.3nM）的电化学生物传感器 ）在过氧化氢的电流检测中的信噪比为3（S / N = 3）。 Au / Pd纳米管SWCNT生物传感器的生物功能化通过硫醇连接在荧光标记的链霉抗生物素蛋白选择性固定在纳米管表面上得到证明。 类似地，葡萄糖氧化酶（GOx）连接到纳米管的表面用于电流葡萄糖感测。 显示的葡萄糖检测限为1.3_M（S / N = 3）和跨越10μM至50 mM的线性范围基本上超过其他基于CNT的生物传感器。 这些结果结合结构与广泛的生物功能化程序的相容性将使得纳米管SWCNT生物传感器在糖尿病患者中非常适用于葡萄糖检测，非常适用于生物标志物的广泛电流检测方案。