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    Recombinant human hepatitis C virus-like particle and method for producing the same
    2.
    发明授权
    Recombinant human hepatitis C virus-like particle and method for producing the same 有权
    重组人类丙型肝炎病毒样颗粒及其制备方法

    公开(公告)号：US08183044B2

    公开(公告)日：2012-05-22

    申请号：US11992646

    申请日：2006-09-29

    申请人： Jun-ichi Tanabe ,  Saburo Sone ,  Takaji Wakita ,  Koji Ishii ,  Ryosuke Suzuki ,  Tetsuro Suzuki ,  Tatsuo Miyamura

    发明人： Jun-ichi Tanabe ,  Saburo Sone ,  Takaji Wakita ,  Koji Ishii ,  Ryosuke Suzuki ,  Tetsuro Suzuki ,  Tatsuo Miyamura

    IPC分类号： C12N15/63

    CPC分类号： C12N7/00 ,  C12N15/86 ,  C12N2770/24223 ,  C12N2770/24243 ,  C12N2770/24252

    摘要： The present invention relates to a method for producing a recombinant hepatitis C virus-like particle comprising the steps of introducing into (i) a cell in which an RNA replicon comprising a nucleotide sequence comprising the 5′ untranslated region, the nucleotide sequence coding for the NS3, NS4A, NS4B, NS5A, and NS5B proteins, and the 3′ untranslated region of a genome RNA derived from a hepatitis C virus strain autonomously replicates, (ii) a vector expressing the Core, E1, E2, and p7 proteins derived from a hepatitis C virus strain that is the same as or different from that as defined in the above (i), culturing the cell, and recovering the produced virus-like particle, and a recombinant hepatitis C virus particle produced by this method.

    摘要翻译： 本发明涉及一种重组丙型肝炎病毒样颗粒的制备方法，包括以下步骤：（i）将包含5'非翻译区的核苷酸序列的RNA复制子，编码 NS3，NS4A，NS4B，NS5A和NS5B蛋白，并且来源于丙型肝炎病毒株的基因组RNA的3'非翻译区自主复制，（ii）表达来源于核苷酸的核心，E1，E2和p7蛋白的载体 与上述（i）所定义的相同或不同的丙型肝炎病毒株，培养细胞，回收所产生的病毒样颗粒，以及通过该方法生产的重组丙型肝炎病毒颗粒。

    High Production System for Infectious Hepatitis C Virus Particle
    3.
    发明申请
    High Production System for Infectious Hepatitis C Virus Particle 失效
    传染性丙型肝炎病毒颗粒高生产系统

    公开(公告)号：US20100035345A1

    公开(公告)日：2010-02-11

    申请号：US11992837

    申请日：2006-09-29

    申请人： Jun-ichi Tanabe ,  Saburo Sone ,  Takaji Wakita ,  Koji Ishii ,  Ryosuke Suzuki ,  Tetsuro Suzuki ,  Tatsuo Miyamura

    发明人： Jun-ichi Tanabe ,  Saburo Sone ,  Takaji Wakita ,  Koji Ishii ,  Ryosuke Suzuki ,  Tetsuro Suzuki ,  Tatsuo Miyamura

    IPC分类号： C12N15/63

    CPC分类号： C12N7/00 ,  C12N15/86 ,  C12N2770/24243 ,  C12N2770/24252

    摘要： The present invention relates to a method for producing infectious hepatitis C virus (HCV) particles, comprising a step of introducing an expression vector into a cell that allows HCV proliferation, such expression vector comprising: DNA sequences encoding the 5′ untranslated region, structural proteins, and, if necessary, non-structural proteins of HCV and DNA sequences encoding non-structural proteins and the 3′ untranslated region derived from the HCV JFH1 strain, which are located downstream of a polymerase I promoter; and a DNA fragment containing an RNA polymerase I terminator, which is located further downstream thereof.

    摘要翻译： 本发明涉及一种生产感染性丙型肝炎病毒（HCV）颗粒的方法，包括将表达载体导入允许HCV增殖的细胞中的步骤，所述表达载体包含：编码5'非翻译区的DNA序列，结构蛋白 ，如果需要，HCV的非结构蛋白和编码非结构蛋白的DNA序列以及来源于HCV JFH1株的3'非翻译区，位于聚合酶I启动子的下游; 和包含位于其下游的RNA聚合酶I终止子的DNA片段。

    Novel Recombinant Human Hepatitis C Virus-Like Particle and Method for Producing the Same
    4.
    发明申请
    Novel Recombinant Human Hepatitis C Virus-Like Particle and Method for Producing the Same 有权
    新型重组人类丙型肝炎病毒样颗粒及其生产方法

    公开(公告)号：US20090221028A1

    公开(公告)日：2009-09-03

    申请号：US11992646

    申请日：2006-09-29

    申请人： Jun-ichi Tanabe ,  Saburo Sone ,  Takaji Wakita ,  Koji Ishii ,  Ryosuke Suzuki ,  Tetsuro Suzuki ,  Tatsuo Miyamura

    发明人： Jun-ichi Tanabe ,  Saburo Sone ,  Takaji Wakita ,  Koji Ishii ,  Ryosuke Suzuki ,  Tetsuro Suzuki ,  Tatsuo Miyamura

    IPC分类号： C12P21/00 ,  C07K14/00

    CPC分类号： C12N7/00 ,  C12N15/86 ,  C12N2770/24223 ,  C12N2770/24243 ,  C12N2770/24252

    摘要： The present invention relates to a method for producing a recombinant hepatitis C virus-like particle comprising the steps of introducing into (i) a cell in which an RNA replicon comprising a nucleotide sequence comprising the 5′ untranslated region, the nucleotide sequence coding for the NS3, NS4A, NS4B, NS5A, and NS5B proteins, and the 3′ untranslated region of a genome RNA derived from a hepatitis C virus strain autonomously replicates, (ii) a vector expressing the Core, E1, E2, and p7 proteins derived from a hepatitis C virus strain that is the same as or different from that as defined in the above (i), culturing the cell, and recovering the produced virus-like particle, and a recombinant hepatitis C virus particle produced by this method.

    摘要翻译： 本发明涉及一种重组丙型肝炎病毒样颗粒的制备方法，包括以下步骤：（i）将包含5'非翻译区的核苷酸序列的RNA复制子，编码 NS3，NS4A，NS4B，NS5A和NS5B蛋白，并且来源于丙型肝炎病毒株的基因组RNA的3'非翻译区自主复制，（ii）表达来源于核苷酸的核心，E1，E2和p7蛋白的载体 与上述（i）所定义的相同或不同的丙型肝炎病毒株，培养细胞，回收所产生的病毒样颗粒，以及通过该方法生产的重组丙型肝炎病毒颗粒。

    SRSV DETECTION KIT
    8.
    发明申请
    SRSV DETECTION KIT 有权
    SRSV检测套件

    公开(公告)号：US20090305420A1

    公开(公告)日：2009-12-10

    申请号：US12498495

    申请日：2009-07-07

    申请人： Naokazu TAKEDA ,  Katsuro Natori ,  Tatsuo Miyamura ,  Kunio Kamata ,  Toshinori Sato ,  Seiya Sato

    发明人： Naokazu TAKEDA ,  Katsuro Natori ,  Tatsuo Miyamura ,  Kunio Kamata ,  Toshinori Sato ,  Seiya Sato

    IPC分类号： C12N15/63 ,  C07H21/04 ,  C07K16/08

    CPC分类号： C07K16/10 ,  C07K14/005 ,  C12N7/00 ,  C12N2770/16021 ,  C12N2770/16022 ,  C12N2770/16023 ,  G01N33/56983 ,  G01N2333/08

    摘要： This invention relates to an SRSV detection kit comprising all antibodies against SRSV-related virus constituting peptides selected from the following peptide groups (a) to (k), respectively: (a) a peptide having an amino acid sequence represented by SEQ ID NO: 1, and the like, (b) a peptide having an amino acid sequence represented by SEQ ID NO: 2, and the like, (c) a peptide having an amino acid sequence represented by SEQ ID NO: 3, and the like, (d) a peptide having an amino acid sequence represented by SEQ ID NO: 4, and the like, (e) a peptide having an amino acid sequence represented by SEQ ID NO: 5, and the like, (f) a peptide having an amino acid sequence represented by SEQ ID NO: 6, and the like, (g) a peptide having an amino acid sequence represented by SEQ ID NO: 7, and the like, (h) a peptide having an amino acid sequence represented by SEQ ID NO: 8, and the like, (i) a peptide having an amino acid sequence represented by SEQ ID NO: 9, and the like, (j) a peptide having an amino acid sequence represented by SEQ ID NO: 10, and the like, and (k) a peptide having an amino acid sequence represented by SEQ ID NO: 11, and the like.Use of the kit makes it possible to detect most SRSV-related viruses and further to distinguish their serotypes and genogroups, easily and surely.

    摘要翻译： 本发明涉及SRSV检测试剂盒，其包含构成分别选自以下肽组（a）至（k）的肽的SRSV相关病毒的所有抗体：（a）具有SEQ ID NO： 1等，（b）具有SEQ ID NO：2所示的氨基酸序列的肽等，（c）具有SEQ ID NO：3所示的氨基酸序列的肽等， （d）具有SEQ ID NO：4所示的氨基酸序列的肽等，（e）具有SEQ ID NO：5所示的氨基酸序列的肽等，（f）具有 由SEQ ID NO：6表示的氨基酸序列等，（g）具有SEQ ID NO：7所示的氨基酸序列的肽等，（h）具有由 SEQ ID NO：8等，（i）具有由SEQ ID NO：9表示的氨基酸序列的肽等，（j）具有 由SEQ ID NO：10表示的次酸酸序列等，和（k）具有SEQ ID NO：11表示的氨基酸序列的肽等。 使用该试剂盒可以检测大多数与SRSV相关的病毒，并进一步区分其血清型和基因组，轻松而可靠。

    SRSV detection kit
    9.
    发明申请
    SRSV detection kit 有权

    公开(公告)号：US20060177820A1

    公开(公告)日：2006-08-10

    申请号：US11402998

    申请日：2006-04-13

    申请人： Naokazu Takeda ,  Katsuro Natori ,  Tatsuo Miyamura ,  Kunio Kamata ,  Toshinori Sato ,  Seiya Sato

    发明人： Naokazu Takeda ,  Katsuro Natori ,  Tatsuo Miyamura ,  Kunio Kamata ,  Toshinori Sato ,  Seiya Sato

    IPC分类号： C12Q1/70 ,  C07K16/10

    CPC分类号： C07K16/10 ,  C07K14/005 ,  C12N7/00 ,  C12N2770/16021 ,  C12N2770/16022 ,  C12N2770/16023 ,  G01N33/56983 ,  G01N2333/08

    摘要： This invention relates to an SRSV detection kit comprising all antibodies against SRSV-related virus constituting peptides selected from the following peptide groups (a) to (k), respectively: (a) a peptide having an amino acid sequence represented by SEQ ID NO: 1, and the like, (b) a peptide having an amino acid sequence represented by SEQ ID NO: 2, and the like, (c) a peptide having an amino acid sequence represented by SEQ ID NO: 3, and the like, (d) a peptide having an amino acid sequence represented by SEQ ID NO: 4, and the like, (e) a peptide having an amino acid sequence represented by SEQ ID NO: 5, and the like, (f) a peptide having an amino acid sequence represented by SEQ ID NO: 6, and the like, (g) a peptide having an amino acid sequence represented by SEQ ID NO: 7, and the like, (h) a peptide having an amino acid sequence represented by SEQ ID NO: 8, and the like, (i) a peptide having an amino acid sequence represented by SEQ ID NO: 9, and the like, (j) a peptide having an amino acid sequence represented by SEQ ID NO: 10, and the like, and (k) a peptide having an amino acid sequence represented by SEQ ID NO: 11, and the like. Use of the kit makes it possible to detect most SRSV-related viruses and further to distinguish their serotypes and genogroups, easily and surely.