摘要:
A process for preparing a therapeutic agent characterized by preculturing human amnion cells isolated from human amnion by trypsinization or human amnion cells obtained by subculturing the isolated cells in a medium containing calf or bovine fetal serum at least one time for 1 to 5 days to form a monolayer, removing the medium, adding a medium containing 0.5 to 20 mg of human serum albumin per 1 ml of medium thereto and culturing it for 3 to 6 days, dialyzing the culture liquid against diluted buffer or water, and filtering the dialyzed culture liquid through a bacterial filter thereby obtaining a germ free liquid agent.
摘要:
A colony-stimulating factor having definite physical and chemical properties and a function of stimulating activity on human bone marrow cells to proliferate and differentiate, thereby forming granulocyte colonies, is obtained from human urine by concentrating the urine with respect to proteins contained therein by adsorption chromatography with silica gel, salting out with ammonium sulfate and other means, then removing impurities by adsorption on cation exchanger, and further purifying by ion exchanging chromatography on anion exchanger, gel filtrating chromatography with highly crosslinked gels, affinitive chromatography with sugar affinitire adsorbents and electrophoresis.
摘要:
Latex reagents for quantitative assay of a variety of steroid hormones or metabolites thereof which are contained in body fluid or excreted fluid of human beings comprise latex particles which are immunologically sensitized with a conjugate selected from a variety of steroid-serum albumin conjugates in which the steroid to be detected is bonded to serum albumin in a very small ratio ranging from 0.5 to 7 molecules per 1 molecule of the serum albumin used.Sensitization of latex particles with such a conjugate is performed using a limited amount dependent on the bonding ratio of steroid molecules per molecule of serum albumin in the conjugate and the particle size of the latex used.The latex reagents show very high sensitivity amounting to 0.2-0.04 nmole steroid equivalent/ml.
摘要:
A colony stimulating factor effective in treating human granulocytopenia is produced by cultivating monocytes and macrophages isolated from the human peripheral blood in a synthetic medium for tissue culture containing a glycoprotein isolated from human urine and capable of stimulating the formation of human granulocytes or mouse macrophages and granulocytes.
摘要:
A colony-stimulating factor having definite physical and chemical properties and a function of stimulating activity on human bone marrow cells to proliferate and differentiate, thereby forming granulocyte colonies, is obtained from human urine by concentrating the urine with respect to proteins contained therein by adsorption chromatography with silica gel, salting out with ammonium sulfate and other means, then removing impurities by adsorption on cation exchanger, and further purifying by ion exchanging chromatography on anion exchanger, gel filtrating chromatography with highly crosslinked gels, affinity chromatography with sugar affinitive adsorbents and electrophoresis. This substance is stable in the presence of a stabilizer such as albumin or urinary proteins, against heat-treatment of virus-inactivation and can be used as a leukopenia curative which is precluded from fear of virus-infection.
摘要:
A method of manufacturing a packaged soybean curd with a long shelf life without the inclusion of any artificial additives such as coagulating agents, germicides and the like; wherein soybean juice is subjected to lactic acid fermentation until its pH is reached to a value equal to or less than a value which is determined from the percentage of the solids content (5-16 wt. %) of soybean juice and then is subjected to heating (60.degree.-95.degree. C., 10-100 minutes) to adjust the curd tension to above 20 g.
摘要:
Packaged aseptic soya bean curd containing no bactericidal agents is manufactured by a method which comprises continuously sterilizing a soya bean juice by maintaining it at a temperature of 128.degree. to 150.degree. C for 1 to 6 seconds and cooling to room temperature; homogeneously mixing said sterilized soya bean juice with a sterilized coagulating agent-containing solution in an aseptic atmosphere continuously or in a pipeline; continuously filling the resulting liquid mixture into a container in an aseptic atmosphere and sealing it; and dipping said sealed container into a water tank heated to 70.degree. to 95.degree. C for 20 to 60 minutes in order to coagulate the mixture.
摘要:
A process for preparing a free-flowing lactulose-containing powder from a solution containing lactose, by adding calcium hydroxide to the solution to adjust the pH of said solution to from 9.4 to 11.2; heating the resulting solution so that the pH is reduced to from 7.5 to 9.0; the homogenizing, concentrating and drying.
摘要:
A granular composition having a grain size of 10 to 50 mesh and a bulk density of 0.5 to 0.7 g/cm.sup.3 suitable for use in a tobacco filter or a tobacco pipe and having the ability to selectively remove carcinogenic and deleterious components from tobacco smoke comprises granules which are composed of bovine milk whey protein power and/or egg white protein powder, and one or more excipients selected from the group consisting of powdered cellulose, wheat flour, starch, rice flour, sugar, glucose, lactose, talc, alumina, zeolite or silica gel; or composite granules which are a blend of said granules and charcoal granules.
摘要翻译:适用于烟草过滤嘴或烟草管并具有从烟草烟雾中选择性除去致癌和有害成分的能力的颗粒大小为10至50目,体积密度为0.5至0.7g / cm 3的颗粒组合物包含颗粒 由牛奶乳清蛋白粉和/或蛋白蛋白粉组成的一种或多种赋形剂,以及选自粉状纤维素,小麦粉,淀粉,米粉,糖,葡萄糖,乳糖,滑石,氧化铝,沸石 或硅胶; 或作为所述颗粒和木炭颗粒的混合物的复合颗粒。
摘要:
A whipped-cream topping dessert is prepared by a process comprising the steps of: (a) formulating an aqueous mixture containing 10 to 30 percent by weight of fat, 3 to 6 percent by weight of milk protein, 12 to 22 percent by weight of saccharide, 0.3 to 0.8 percent by weight of emulsifier, 0.05 to 0.15 percent by weight of thickening agent and 0.3 to 0.6 percent by weight of gelling agent; (b) homogenizing said mixture; (c) pasteurizing and cooling the mixture to room temperature; (d) thereafter adding an aqueous solution of a calcium salt to said mixture so that the amount of calcium ion in said mixture is 10 to 35 mg. per 1 gram of the milk protein; (e) whipping and heating the resultant mixture at a temperature of 25.degree. to 30.degree.C to prepare a flowable whipped-cream; (f) topping said whipped-cream onto a lower layer dessert base maintained at a temperature of 50.degree. to 70.degree.C which has previously been placed into a container; and (g) solidifying both layers by cooling.