公开(公告)号：US07932083B2

公开(公告)日：2011-04-26

申请号：US10577655

申请日：2004-11-25

申请人： Kazuhiro Machida ,  Takashi Nakashima ,  Yasuhide Aritoku ,  Toshio Tsuchida

发明人： Kazuhiro Machida ,  Takashi Nakashima ,  Yasuhide Aritoku ,  Toshio Tsuchida

IPC分类号： C12N5/00 ,  C12N15/00 ,  C12P21/06 ,  C07H21/02 ,  C07H21/04

CPC分类号： C12P17/08 ,  C12N9/10

摘要： The present invention provides a DNA participating in biological transformation of a macrolide compound 11107B. The present invention provides, particularly, a DNA participating in biological transformation of a macrolide compound 11107B represented by the formula (I) into a 16-position hydroxy macrolide compound 11107D represented by the formula (II), the DNA encoding a protein having 16-position hydroxylating enzymatic activity or ferredoxin, to a method of isolating the DNA, to a protein encoded by the DNA, a plasmid carrying the DNA, a transformant obtained by transforming using the plasmid and a method of producing a 16-position hydroxy compound by using the transformant.

摘要翻译： 本发明提供参与大环内酯化合物11107B的生物转化的DNA。 本发明特别提供参与由式（I）表示的大环内酯类化合物11107B生成转化为式（II）所示的16位羟基大环内酯化合物11107D的DNA，编码具有16位的式 位置羟化酶活性或铁氧还蛋白，分离DNA的方法，由DNA编码的蛋白质，携带DNA的质粒，通过使用质粒转化获得的转化体和通过使用制备16位羟基化合物的方法 转化子。

公开(公告)号：US20080070286A1

公开(公告)日：2008-03-20

申请号：US10577655

申请日：2004-11-25

申请人： Kazuhiro Machida ,  Yasuhide Aritoku ,  Toshio Tsuchida ,  Takashi Nakashima

发明人： Kazuhiro Machida ,  Yasuhide Aritoku ,  Toshio Tsuchida ,  Takashi Nakashima

IPC分类号： C12P17/08 ,  C07H21/04 ,  C12N15/00 ,  C12N15/11 ,  C12N5/06 ,  C12N9/14

CPC分类号： C12P17/08 ,  C12N9/10

摘要： The present invention provides a DNA participating in biological transformation of a macrolide compound 11107B. The present invention provides, particularly, a DNA participating in biological transformation of a macrolide compound 11107B represented by the formula (I) into a 16-position hydroxy macrolide compound 11107D represented by the formula (II), the DNA encoding a protein having 16-position hydroxylating enzymatic activity or ferredoxin, to a method of isolating the DNA, to a protein encoded by the DNA, a plasmid carrying the DNA, a transformant obtained by transforming using the plasmid and a method of producing a 16-position hydroxy compound by using the transformant.

摘要翻译： 本发明提供参与大环内酯化合物11107B的生物转化的DNA。 本发明特别提供参与由式（I）表示的大环内酯类化合物11107B生成转化为式（II）所示的16位羟基大环内酯化合物11107D的DNA，编码具有16位的式 位置羟化酶活性或铁氧还蛋白，分离DNA的方法，由DNA编码的蛋白质，携带DNA的质粒，通过使用质粒转化获得的转化体和通过使用制备16位羟基化合物的方法 转化子。

公开(公告)号：US20090215134A1

公开(公告)日：2009-08-27

申请号：US11919579

申请日：2006-05-24

申请人： Kazuhiro Machida ,  Yasuhide Aritoku

发明人： Kazuhiro Machida ,  Yasuhide Aritoku

IPC分类号： C12P17/16 ,  C12N1/21 ,  C07H21/04 ,  C07K2/00

CPC分类号： C12N15/52 ,  C12N9/0071 ,  C12P17/08 ,  C12P17/162

摘要： A genetically modified microorganism capable of directly producing a 16-hydroxylated macrolide compound; and a process for producing a 16-hydroxylated macrolide compound using the microorganism. Specifically, a genetically modified microorganisms having DNA encoding a polypeptide involved in the biosynthesis of a macrolide compound pladienolide and DNA encoding a polypeptide having a pladienolide 16-hydroxylase activity; and a process for producing a 16-hydroxylated macrolide compound comprising the steps of culturing the genetically modified microorganism in a culture medium and collecting a 16-hydroxylated macrolide compound from the culture medium.

摘要翻译： 能够直接生产16-羟化大环内酯化合物的转基因微生物; 以及使用该微生物生产16-羟基化大环内酯类化合物的方法。 具体地，涉及编码参与大环内酯化合物扁桃多烯内酯的生物合成的多肽的DNA的遗传修饰的微生物和编码具有扁桃二醇内酯16-羟化酶活性的多肽的DNA; 以及生产16-羟基化大环内酯类化合物的方法，其包括在培养基中培养经遗传修饰的微生物并从培养基中收集16-羟化大环内酯类化合物的步骤。

公开(公告)号：US08008049B2

公开(公告)日：2011-08-30

申请号：US11630689

申请日：2005-07-19

申请人： Kazuhiro Machida ,  Akira Arisawa ,  Susumu Takeda ,  Masashi Yoshida ,  Toshio Tsuchida

发明人： Kazuhiro Machida ,  Akira Arisawa ,  Susumu Takeda ,  Masashi Yoshida ,  Toshio Tsuchida

IPC分类号： C12P17/16 ,  C07H21/04 ,  C07K2/00 ,  C12N9/00 ,  C12N15/63 ,  C12N1/00

CPC分类号： C12P17/08 ,  C07K14/36 ,  C12N9/0071 ,  C12N9/0073 ,  C12N15/52 ,  C12P17/162

摘要： The present invention provides polypeptides that participate in the biosynthesis of the pladienolide macrolide compounds, DNA that encodes these polypeptides and variants of this DNA, transformants that maintain all or a portion of this DNA or variant thereof, and a method of producing the pladienolide macrolide compounds using these transformants. More particularly, it provides an isolated pure DNA that contains at least one region encoding a polypeptide that participates in pladienolide biosynthesis; polypeptide encoded by this DNA; a self-replicating or integrated-replicating recombinant plasmid carrying this DNA; a transformant maintaining this DNA; and a method of producing a pladienolide, characterized by culturing this transformant on culture medium and collecting pladienolide from this culture medium.

摘要翻译： 本发明提供了参与扁平二烯内酰脲大环内酯化合物的生物合成的多肽，编码这些多肽的DNA和该DNA的变体，保持该DNA或其变体的全部或一部分的转化体，以及生产该扁桃苷内酰胺大环内酯化合物的方法 使用这些转化体。 更具体地，其提供了分离的纯DNA，其含有至少一个编码参与泛二烯内酯生物合成的多肽的区域; 由该DNA编码的多肽; 携带该DNA的自我复制或整合复制重组质粒; 维持该DNA的转化体 以及生产普拉二烯内酯的方法，其特征在于在培养基上培养该转化体并从该培养基中收集普拉二烯内酯。

公开(公告)号：US20090269820A1

公开(公告)日：2009-10-29

申请号：US11630689

申请日：2005-07-19

申请人： Kazuhiro Machida ,  Akira Arisawa ,  Susumu Takeda ,  Masashi Yoshida ,  Toshio Tsuchida

发明人： Kazuhiro Machida ,  Akira Arisawa ,  Susumu Takeda ,  Masashi Yoshida ,  Toshio Tsuchida

IPC分类号： C12P17/16 ,  C07H21/04 ,  C07K2/00 ,  C12N9/00 ,  C12N15/63 ,  C12N1/00

CPC分类号： C12P17/08 ,  C07K14/36 ,  C12N9/0071 ,  C12N9/0073 ,  C12N15/52 ,  C12P17/162

摘要： The present invention provides polypeptides that participate in the biosynthesis of the pladienolide macrolide compounds, DNA that encodes these polypeptides and variants of this DNA, transformants that maintain all or a portion of this DNA or variant thereof, and a method of producing the pladienolide macrolide compounds using these transformants. More particularly, it provides an isolated pure DNA that contains at least one region encoding a polypeptide that participates in pladienolide biosynthesis; polypeptide encoded by this DNA; a self-replicating or integrated-replicating recombinant plasmid carrying this DNA; a transformant maintaining this DNA; and a method of producing a pladienolide, characterized by culturing this transformant on culture medium and collecting pladienolide from this culture medium.

摘要翻译： 本发明提供了参与扁平二烯内酰脲大环内酯化合物的生物合成的多肽，编码这些多肽的DNA和该DNA的变体，保持该DNA或其变体的全部或一部分的转化体，以及生产该扁桃苷内酰胺大环内酯化合物的方法 使用这些转化体。 更具体地，其提供了分离的纯DNA，其含有至少一个编码参与泛二烯内酯生物合成的多肽的区域; 由该DNA编码的多肽; 携带该DNA的自我复制或整合复制重组质粒; 维持该DNA的转化体 以及生产普拉二烯内酯的方法，其特征在于在培养基上培养该转化体并从该培养基中收集普拉二烯内酯。

公开(公告)号：US06262721B1

公开(公告)日：2001-07-17

申请号：US08888362

申请日：1997-07-03

申请人： Ryota Tsukidate ,  Yoshiyasu Takeuchi ,  Kazuhiro Machida ,  Takenosuke Harada ,  Shigeki Kaneko ,  Kenichi Fujita ,  Yoshimasa Goto

发明人： Ryota Tsukidate ,  Yoshiyasu Takeuchi ,  Kazuhiro Machida ,  Takenosuke Harada ,  Shigeki Kaneko ,  Kenichi Fujita ,  Yoshimasa Goto

IPC分类号： H04N710

CPC分类号： H04H20/33 ,  H04H20/106 ,  H04H20/16 ,  H04H20/28 ,  H04H20/30 ,  H04H20/38 ,  H04H20/40 ,  H04H20/76 ,  H04H20/93 ,  H04H60/06 ,  H04H60/15 ,  H04H60/16 ,  H04H60/17 ,  H04H60/21 ,  H04H60/27 ,  H04H60/31 ,  H04H60/47 ,  H04H60/51 ,  H04H60/66 ,  H04N7/0887 ,  H04N7/163 ,  H04N21/235 ,  H04N21/25891 ,  H04N21/2668 ,  H04N21/435 ,  H04N21/44204 ,  H04N21/4532 ,  H04N21/454 ,  H04N21/4755 ,  H04N21/4758 ,  H04N21/6582 ,  H04N21/84 ,  H04N21/8405 ,  H04N21/8586

摘要： Viewer individual attribute information indicating an attribute of a particular viewer such as an age, male or female, a resident district, a taste, an occupation and a life style is stored in a receiving terminal apparatus of the particular viewer. When a broadcasting program is transmitted from a service supply apparatus to the receiving terminal apparatus, service addition information indicating an attribute of the broadcasting program or an attribute condition required of viewers is transmitted to the receiving terminal apparatus with contents of the broadcasting program. In the receiving terminal apparatus, the service addition information is collated with the viewer individual attribute information of the particular viewer, and it is judged whether or not the broadcasting program is suitable for the particular viewer. In cases where the broadcasting program is suitable for the particular viewer, the broadcasting program is received by the receiving terminal apparatus and is reproduced and displayed. Therefore, the broadcasting program can be received by only a group of particular viewers desired on a side of the service supply apparatus even though the group of particular viewers is not specified.

摘要翻译： 在特定观看者的接收终端装置中存储有表示特定观看者的属性（诸如年龄，男性或女性，居民区，品味，职业和生活方式）的观众个人属性信息。 当从服务提供装置向接收终端装置发送广播节目时，以广播节目的内容向接收终端装置发送指示广播节目的属性的服务添加信息或观众所需的属性条件。 在接收终端装置中，将服务添加信息与特定观众的观众个人属性信息进行核对，并且判断广播节目是否适合于特定观众。 在广播节目适合于特定观看者的情况下，广播节目由接收终端装置接收并被再现和显示。 因此，尽管没有指定特定观看者组，但是广播节目只能由服务提供设备侧所需的一组特定观看者接收。

公开(公告)号：US06035304A

公开(公告)日：2000-03-07

申请号：US881235

申请日：1997-06-24

申请人： Kazuhiro Machida ,  Takenosuke Harada ,  Shigeki Kaneko ,  Ryota Tsukidate ,  Yoshiyasu Takeuchi ,  Kenichi Fujita

发明人： Kazuhiro Machida ,  Takenosuke Harada ,  Shigeki Kaneko ,  Ryota Tsukidate ,  Yoshiyasu Takeuchi ,  Kenichi Fujita

IPC分类号： G06F17/30 ,  H04N7/16 ,  H04N7/24

CPC分类号： H04N21/4332 ,  G06F17/30017 ,  H04N21/2362 ,  H04N21/4307 ,  H04N21/4331 ,  H04N21/4345 ,  H04N21/4348 ,  H04N21/4722 ,  H04N21/47815 ,  H04N21/4784 ,  H04N21/812 ,  H04N21/8173 ,  H04N21/8549 ,  H04N21/858 ,  H04N7/162 ,  H04N7/24 ,  Y10S707/99945 ,  Y10S707/99948

摘要： A system capable of storing the application packages and thereafter reproducing a desired one of the stored application packages at any time. The application package comprises a plurality of applications. Each of the applications comprises application data which comprises forms a main body of the application and service adding information (SAI) comprising various data including an application ID of the application. The SAIs are distributed independently from and prior to the application data. Each of the SAIs can include method IDs of methods for processing object data specified by a method processing program and a plurality of link data comprising application IDs of other applications. This permits the SAIs to form a tree structure of the applications. The methods are activated in a event driven manner.

摘要翻译： 一种能够存储应用程序包并随后在任何时间再现所存储的应用程序包的所需一个的系统。 应用程序包包括多个应用程序。 每个应用包括应用数据，其包括形成应用的主体和包括包括应用的应用ID的各种数据的服务添加信息（SAI）。 SAI是在应用数据之前和之前独立分发的。 每个SAI可以包括用于处理由方法处理程序指定的对象数据的方法的方法ID和包括其他应用的应用ID的多个链接数据。 这允许SAI形成应用程序的树结构。 这些方法以事件驱动方式激活。

公开(公告)号：US20120040441A1

公开(公告)日：2012-02-16

申请号：US12449868

申请日：2008-02-28

申请人： Tadashi Fujii ,  Atsushi Ochiai ,  Masashi Ito ,  Hiroki Kabumoto ,  Yoshikazu Fujii ,  Kazuhiro Machida

发明人： Tadashi Fujii ,  Atsushi Ochiai ,  Masashi Ito ,  Hiroki Kabumoto ,  Yoshikazu Fujii ,  Kazuhiro Machida

IPC分类号： C12N1/21

CPC分类号： C12P1/04 ,  C07K14/195 ,  C12P7/02 ,  C12P7/62 ,  C12P13/08 ,  C12P17/10 ,  C12P33/06

摘要： Disclosed is a means for improving the poor conversion efficiency in a conventional bioconversion system using a transformant which is given by introducing a gene originated from xerogenic organisms. A transformant is prepared by using a host which is defective in a gene encoding a multidrug efflux protein and introducing a gene originated from xerogenic organisms. Use of the transformant results in much effective microbial conversion of a hydrophobic or amphipathic substrate compound into a desired compound. In case, an Escherichia coli is used as the host, the gene encoding a multidrug efflux protein to be defective may be tolC, acrA, acrB and the like.

摘要翻译： 公开了一种改善常规生物转化系统中转化效率差的方法，该方法是使用通过引入源自干细胞生物体的基因而给出的转化体。 通过使用在编码多药外排蛋白的基因中有缺陷的宿主并引入源自干细胞生物的基因来制备转化体。 转化体的使用导致将疏水性或两亲性底物化合物转化为所需化合物的有效微生物转化。 如果使用大肠杆菌作为宿主，编码有缺陷的多药外排蛋白的基因可以是toC，acrA，acrB等。

公开(公告)号：US20100285565A1

公开(公告)日：2010-11-11

申请号：US12449876

申请日：2008-02-28

申请人： Tadashi Fujii ,  Yoshikazu Fujii ,  Atsushi Ochiai ,  Masashi Ito ,  Kazuhiro Machida

发明人： Tadashi Fujii ,  Yoshikazu Fujii ,  Atsushi Ochiai ,  Masashi Ito ,  Kazuhiro Machida

IPC分类号： C12N9/02 ,  C12N15/70 ,  C12N1/21

CPC分类号： C12P7/18 ,  C12N9/0077

摘要： Disclosed is a means for improving the poor conversion efficiency in a bioconversion system using an Escherichia coli cell having a bacterium-originated cytochrome P-450 gene integrated therein. A recombinant Escherichia coli cell is produced by introducing aciB and aciC which encode a gene for the electron transport system originated from the Acinetobacter sp. OC4 strain into an Escherichia coli cell, and adding a polynucleotide encoding an N-terminal sequence composed of 48 amino acid residues of AciA and the like to the 5′-terminus of a bacterium-originated cytochrome P-450 gene, wherein AciA is an alkane-oxidative cytochrome P-450 originated from the Acinetobacter sp. OC4 strain. Use of the recombinant Escherichia coli cell results in much effective microbial conversion of a hydrophobic or amphipathic substrate compound into a desired compound.

摘要翻译： 公开了一种用于提高生物转化系统中转化效率差的方法，该系统使用其中整合有细菌色素细胞色素P-450基因的大肠杆菌细胞。 通过引入编码源自不动杆菌属的电子传递系统的基因的aciB和aciC来产生重组大肠杆菌细胞。 OC4菌株加入到大肠杆菌细胞中，并将编码由AciA等的48个氨基酸残基组成的N末端序列的多核苷酸加入到细菌来源的细胞色素P-450基因的5'末端，其中AciA为 链烷氧化细胞色素P-450源自不动杆菌属。 OC4菌株。 重组大肠杆菌细胞的使用导致疏水性或两亲性底物化合物成为所需化合物的有效微生物转化。

公开(公告)号：US06714649B1

公开(公告)日：2004-03-30

申请号：US09345829

申请日：1999-07-01

申请人： Isao Masuda ,  Yoshimasa Goto ,  Takenosuke Harada ,  Kazuhiro Machida ,  Mitsuteru Kataoka

发明人： Isao Masuda ,  Yoshimasa Goto ,  Takenosuke Harada ,  Kazuhiro Machida ,  Mitsuteru Kataoka

IPC分类号： H04L944

CPC分类号： H04H60/23 ,  H04H60/15 ,  H04N5/913 ,  H04N7/1675 ,  H04N21/2347 ,  H04N21/4334 ,  H04N21/4405 ,  H04N21/63345 ,  H04N2005/91364

摘要： In a pay broadcasting system, data of a broadcast program is scrambled with a scramble key updated in a short period. The scramble key is encrypted with a first key assigned to the subscriber terminal. The first key is encrypted with a first master key set in the subscriber terminal. The security of a broadcast program stored for subsequent use in the subscriber terminal is enhanced as follows. A central station generates a second key-encrypted scramble key by encrypting the scramble key with a second key different from the first key and changeable in an interval shorter than a update interval of the first key; generates an encrypted second key by encrypting the second key with a second master key which has been commonly issued to subscriber terminals of the system; and broadcast the second key-encrypted scramble key and the encrypted second key together with the scrambled data of the program, the first key-encrypted scramble key and the encrypted first key in a multiplexed manner. When a broadcast program is to be stored, the subscriber terminal stores the scrambled data of the broadcast program and the second key-encrypted scramble key; decrypt the encrypted second key with the second master key into the second key, which is added to a stored program second key list. If the stored program is to be executed, the second key-encrypted scramble key is decrypted with a corresponding one of the second keys in the stored program second key list into a decrypted scramble key; and the scrambled data of the broadcast program is unscrambled with the decrypted scramble key.

摘要翻译： 在付费广播系统中，广播节目的数据用短时间更新的加扰密钥加扰。 加密密钥用分配给用户终端的第一密钥加密。 第一密钥是用在用户终端中设置的第一主密钥进行加密的。 存储用于用户终端中的后续使用的广播节目的安全性如下增强。 中央站通过用不同于第一密钥的第二密钥加密加密密钥并且以比第一密钥的更新间隔短的间隔进行加密来生成第二密钥加密的加密密钥; 通过用通常发给系统的用户终端的第二主密钥加密第二密钥来生成加密的第二密钥; 并且以多路复用的方式与节目的加密数据，第一密钥加密的加扰密钥和加密的第一密钥一起广播第二密钥加密的加密密钥和加密的第二密钥。 当要存储广播节目时，用户终端存储广播节目的加密数据和第二密钥加密的加扰密钥; 将具有第二主密钥的加密的第二密钥解密为第二密钥，其被添加到存储的程序第二密钥列表。 如果要执行存储的程序，则将第二密钥加密的加密密钥与存储的程序第二密钥列表中的第二密钥中的相应的一个密钥解密成解密的加密密钥; 并且利用解密的加扰密钥对广播节目的加密数据进行解扰。