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    ESCHERICHIA COLI EXPRESSING THE CYTOCHROME P-450 GENE AND A METHOD FOR MICROBIAL CONVERSION USING THEM
    3.
    发明申请
    ESCHERICHIA COLI EXPRESSING THE CYTOCHROME P-450 GENE AND A METHOD FOR MICROBIAL CONVERSION USING THEM 有权
    表达CYTOCHROME P-450基因的ESCHERICHIA COLI和使用它们的微生物转化方法

    公开(公告)号：US20100285565A1

    公开(公告)日：2010-11-11

    申请号：US12449876

    申请日：2008-02-28

    申请人： Tadashi Fujii ,  Yoshikazu Fujii ,  Atsushi Ochiai ,  Masashi Ito ,  Kazuhiro Machida

    发明人： Tadashi Fujii ,  Yoshikazu Fujii ,  Atsushi Ochiai ,  Masashi Ito ,  Kazuhiro Machida

    IPC分类号： C12N9/02 ,  C12N15/70 ,  C12N1/21

    CPC分类号： C12P7/18 ,  C12N9/0077

    摘要： Disclosed is a means for improving the poor conversion efficiency in a bioconversion system using an Escherichia coli cell having a bacterium-originated cytochrome P-450 gene integrated therein. A recombinant Escherichia coli cell is produced by introducing aciB and aciC which encode a gene for the electron transport system originated from the Acinetobacter sp. OC4 strain into an Escherichia coli cell, and adding a polynucleotide encoding an N-terminal sequence composed of 48 amino acid residues of AciA and the like to the 5′-terminus of a bacterium-originated cytochrome P-450 gene, wherein AciA is an alkane-oxidative cytochrome P-450 originated from the Acinetobacter sp. OC4 strain. Use of the recombinant Escherichia coli cell results in much effective microbial conversion of a hydrophobic or amphipathic substrate compound into a desired compound.

    摘要翻译： 公开了一种用于提高生物转化系统中转化效率差的方法，该系统使用其中整合有细菌色素细胞色素P-450基因的大肠杆菌细胞。 通过引入编码源自不动杆菌属的电子传递系统的基因的aciB和aciC来产生重组大肠杆菌细胞。 OC4菌株加入到大肠杆菌细胞中，并将编码由AciA等的48个氨基酸残基组成的N末端序列的多核苷酸加入到细菌来源的细胞色素P-450基因的5'末端，其中AciA为 链烷氧化细胞色素P-450源自不动杆菌属。 OC4菌株。 重组大肠杆菌细胞的使用导致疏水性或两亲性底物化合物成为所需化合物的有效微生物转化。

    Escherichia coli expressing the cytochrome P-450 gene and a method for microbial conversion using them
    4.
    发明授权
    Escherichia coli expressing the cytochrome P-450 gene and a method for microbial conversion using them 有权
    表达细胞色素P-450基因的大肠杆菌和使用它们的微生物转化方法

    公开(公告)号：US08460915B2

    公开(公告)日：2013-06-11

    申请号：US12449876

    申请日：2008-02-28

    申请人： Tadashi Fujii ,  Yoshikazu Fujii ,  Atsushi Ochiai ,  Masashi Ito ,  Kazuhiro Machida

    发明人： Tadashi Fujii ,  Yoshikazu Fujii ,  Atsushi Ochiai ,  Masashi Ito ,  Kazuhiro Machida

    IPC分类号： C12N9/00 ,  C12N9/02 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04

    CPC分类号： C12P7/18 ,  C12N9/0077

    摘要： Disclosed is a means for improving the poor conversion efficiency in a bioconversion system using an Escherichia coli cell having a bacterium-originated cytochrome P-450 gene integrated therein. A recombinant Escherichia coli cell is produced by introducing aciB and aciC which encode a gene for the electron transport system originated from the Acinetobacter sp. OC4 strain into an Escherichia coli cell, and adding a polynucleotide encoding an N-terminal sequence composed of 48 amino acid residues of AciA and the like to the 5′-terminus of a bacterium-originated cytochrome P-450 gene, wherein AciA is an alkane-oxidative cytochrome P-450 originated from the Acinetobacter sp. OC4 strain. Use of the recombinant Escherichia coli cell results in much effective microbial conversion of a hydrophobic or amphipathic substrate compound into a desired compound.

    摘要翻译： 公开了一种用于提高生物转化系统中转化效率差的方法，该系统使用其中整合有细菌色素细胞色素P-450基因的大肠杆菌细胞。 通过引入编码源自不动杆菌属的电子传递系统的基因的aciB和aciC来产生重组大肠杆菌细胞。 OC4菌株加入到大肠杆菌细胞中，并将编码由AciA等的48个氨基酸残基组成的N末端序列的多核苷酸加入到细菌来源的细胞色素P-450基因的5'末端，其中AciA为 链烷氧化细胞色素P-450源自不动杆菌属。 OC4菌株。 重组大肠杆菌细胞的使用导致疏水性或两亲性底物化合物成为所需化合物的有效微生物转化。

    STEROL SIDE CHAIN-CLEAVING ENZYME PROTEIN AND USE THEREOF
    5.
    发明申请
    STEROL SIDE CHAIN-CLEAVING ENZYME PROTEIN AND USE THEREOF 审中-公开
    STEROL侧链酶清除酶蛋白及其用途

    公开(公告)号：US20120178124A1

    公开(公告)日：2012-07-12

    申请号：US13143607

    申请日：2009-01-07

    申请人： Masahiro Yamagishi ,  Takeshi Sakamoto ,  Makoto Ueda ,  Masashi Itoh ,  Yoshikazu Fujii ,  Hiroki Kabumoto ,  Akira Arisawa ,  Tadashi Fujii

    发明人： Masahiro Yamagishi ,  Takeshi Sakamoto ,  Makoto Ueda ,  Masashi Itoh ,  Yoshikazu Fujii ,  Hiroki Kabumoto ,  Akira Arisawa ,  Tadashi Fujii

    IPC分类号： C12P33/00 ,  C12N15/52 ,  C12N1/15 ,  C12N9/96 ,  C12N1/21 ,  C12N1/19 ,  C12N9/00 ,  C12N15/63

    CPC分类号： C12N9/0077 ,  C12N9/0081 ,  C12N15/70 ,  C12N15/75 ,  C12P33/12

    摘要： It is an object of the present invention to obtain highly active P450scc enzyme protein which is an important enzyme protein that catalyzes the first step of the biosynthesis of industrially useful steroid hormone. The present invention provides a sterol side chain cleavage enzyme protein having the following physicochemical properties: (1) action: the enzyme acts on sterol represented by formula (I) as defined in the specification and cleaves the carbon-carbon bond between positions 20 and 22 of a sterol side chain portion by its activity of cleaving the bonds, so as to generate a compound represented by formula (II) as defined in the specification; (2) substrate specificity: when microorganisms that produce the enzyme protein are allowed to react with an aqueous solution containing 100 μg/ml 4-cholesten-3-one or cholesterol at 28° C. for 5 hours, the conversion reaction rate from 4-cholesten-3-one to progesterone is 10% or more, and the conversion rate from cholesterol to pregnenolone is 10% or more;

    摘要翻译： 本发明的目的是获得高活性P450scc酶蛋白，其是催化工业上有用的类固醇激素生物合成的第一步的重要酶蛋白。 本发明提供了具有以下物理化学性质的甾醇侧链切割酶蛋白：（1）作用：酶作用于如说明书中定义的由式（I）表示的固醇，并切割位置20和22之间的碳 - 碳键 的甾醇侧链部分通过其切割键的活性，从而产生如说明书中定义的式（II）表示的化合物; （2）底物特异性：使得产生酶蛋白的微生物在28℃下与含有100μg/ ml 4-胆甾烯-3-酮或胆固醇的水溶液反应5小时时，转化反应速率为4 胆固醇-3-酮与孕酮的含量为10％以上，胆固醇与孕烯醇酮的转化率为10％以上;

    EXPRESSION VECTOR FOR PSEUDONOCARDIA AUTOTROPHICA
    6.
    发明申请
    EXPRESSION VECTOR FOR PSEUDONOCARDIA AUTOTROPHICA 有权
    PSEUDONOCARDIA AUTOTROPHICA的表达向量

    公开(公告)号：US20110262978A1

    公开(公告)日：2011-10-27

    申请号：US13122675

    申请日：2009-10-05

    申请人： Yoshikazu Fujii ,  Tadashi Fujii ,  Akira Arisawa ,  Tomohiro Tamura

    发明人： Yoshikazu Fujii ,  Tadashi Fujii ,  Akira Arisawa ,  Tomohiro Tamura

    IPC分类号： C12P7/02 ,  C12N15/74 ,  C12N9/02 ,  C12P7/62 ,  C12N15/63 ,  C12N1/21

    CPC分类号： C12P21/02 ,  C12N9/0071 ,  C12N9/0073 ,  C12N15/74 ,  C12P7/02 ,  C12P7/62 ,  C12P17/06 ,  C12Y114/13013

    摘要： An expression vector capable of expressing a foreign gene in Pseudonocardia autotrophica; a transformant of Pseudonocardia autotrophica produced by using the expression vector; a method for producing a protein by using the transformant; a method for producing an active form of vitamin D3 from vitamin D3, which comprises highly expressing a gene encoding an enzyme involved in the synthesis of the active form of vitamin D3 in a transformant by using the expression vector or the transformant; a method for producing 25-hydroxyvitamin D2 from vitamin D2; and a method for producing pravastatin from compactin, which comprises highly expressing a compactin hydroxylase gene in a transformant by using the expression vector or the transformant.

    摘要翻译： 能够在自养假诺卡氏菌中表达外源基因的表达载体; 通过使用表达载体产生的假心内膜自养基因的转化体; 通过使用该转化体生产蛋白质的方法; 从维生素D3生产活性形式的维生素D3的方法，其包括通过使用表达载体或转化体高度表达编码参与转化体中活性形式的维生素D3的活性形式的酶的基因; 从维生素D2生产25-羟基维生素D 2的方法; 以及通过使用表达载体或转化体高效表达转化体中的致密蛋白羟化酶基因的来自美伐他汀的普伐他汀的制造方法。

    Expression vector for pseudonocardia autotrophica
    7.
    发明授权
    Expression vector for pseudonocardia autotrophica 有权
    假诺卡氏菌属的表达载体

    公开(公告)号：US09006412B2

    公开(公告)日：2015-04-14

    申请号：US13122675

    申请日：2009-10-05

    申请人： Yoshikazu Fujii ,  Tadashi Fujii ,  Akira Arisawa ,  Tomohiro Tamura

    发明人： Yoshikazu Fujii ,  Tadashi Fujii ,  Akira Arisawa ,  Tomohiro Tamura

    IPC分类号： C07H21/04 ,  C12N15/00 ,  C12N15/64 ,  C12P21/06 ,  C12P21/02 ,  C12N9/02 ,  C12N15/74 ,  C12P7/02 ,  C12P7/62 ,  C12P17/06

    CPC分类号： C12P21/02 ,  C12N9/0071 ,  C12N9/0073 ,  C12N15/74 ,  C12P7/02 ,  C12P7/62 ,  C12P17/06 ,  C12Y114/13013

    摘要： An expression vector capable of expressing a foreign gene in Pseudonocardia autotrophica; a transformant of Pseudonocardia autotrophica produced by using the expression vector; a method for producing a protein by using the transformant; a method for producing an active form of vitamin D3 from vitamin D3, which comprises highly expressing a gene encoding an enzyme involved in the synthesis of the active form of vitamin D3 in a transformant by using the expression vector or the transformant; a method for producing 25-hydroxyvitamin D2 from vitamin D2; and a method for producing pravastatin from compactin, which comprises highly expressing a compactin hydroxylase gene in a transformant by using the expression vector or the transformant.

    摘要翻译： 能够在自养假诺卡氏菌中表达外源基因的表达载体; 通过使用表达载体产生的假心内膜自养基因的转化体; 通过使用该转化体生产蛋白质的方法; 从维生素D3生产活性形式的维生素D3的方法，其包括通过使用表达载体或转化体高度表达编码参与转化体中活性形式的维生素D3的活性形式的酶的基因; 从维生素D2生产25-羟基维生素D 2的方法; 以及通过使用表达载体或转化体高效表达转化体中的致密蛋白羟化酶基因的来自美伐他汀的普伐他汀的制造方法。

    Motors containing segment conductor coils
    8.
    发明授权
    Motors containing segment conductor coils 有权
    包含分段导体线圈的电机

    公开(公告)号：US08916999B2

    公开(公告)日：2014-12-23

    申请号：US13335000

    申请日：2011-12-22

    申请人： Hiroshi Imai ,  Yusuke Tateishi ,  Keisuke Koide ,  Tatsunori Banba ,  Takumi Suzuki ,  Yoshikazu Fujii

    发明人： Hiroshi Imai ,  Yusuke Tateishi ,  Keisuke Koide ,  Tatsunori Banba ,  Takumi Suzuki ,  Yoshikazu Fujii

    IPC分类号： H02K11/00 ,  H02K5/00 ,  H02K21/12 ,  H02K3/00 ,  H02K3/28 ,  H02K1/27 ,  H02K3/50 ,  H02K5/22 ,  H02K21/14 ,  H02K29/03

    CPC分类号： H02K3/28 ,  H02K1/2746 ,  H02K3/50 ,  H02K5/225 ,  H02K11/33 ,  H02K21/14 ,  H02K29/03 ,  H02K2201/06 ,  H02K2213/03

    摘要： A motor includes a motor case, and a rotor and a stator, which are disposed in the case. The case has a tubular portion, a front cover mounted to an axial end of the portion, and a rear cover mounted to the other axial end of the portion. The rotor has a rotary shaft and the stator includes a plurality of teeth, which extend toward a central axis of the shaft and are circumferentially disposed at equal intervals. Between each circumferentially adjacent pair of the teeth, a slot extending toward the axis is formed. In each slot, a U-shaped segment is inserted in parallel with the axis. The distal ends of the segments projecting out from the slots are electrically interconnected, thereby forming an SC coil including the segments disposed circumferentially. The SC coil includes a receiving terminal, and the terminal includes leads extending in parallel to the axis.

    摘要翻译： 电动机包括设置在壳体中的电动机壳体，转子和定子。 壳体具有管状部分，安装到该部分的轴向端部的前盖以及安装到该部分的另一个轴向端部的后盖。 转子具有旋转轴，并且定子包括多个齿，其朝向轴的中心轴线延伸并以等间隔周向设置。 在每个周向相邻的齿对之间形成有朝向轴延伸的槽。 在每个槽中，U形段与轴平行地插入。 从槽突出的段的远端电互连，从而形成包括周向设置的段的SC线圈。 SC线圈包括接收端子，并且该端子包括平行于该轴线延伸的引线。

    (Meth) acrylic acid type polymer and unsaturated polyalkylene glycol type copolymer, and methods for production thereof
    9.
    发明授权
    (Meth) acrylic acid type polymer and unsaturated polyalkylene glycol type copolymer, and methods for production thereof 有权
    （甲基）丙烯酸型聚合物和不饱和聚亚烷基二醇型共聚物及其制备方法

    公开(公告)号：US07132487B2

    公开(公告)日：2006-11-07

    申请号：US11216772

    申请日：2005-08-31

    申请人： Atsuro Yoneda ,  Takahiro Tsumori ,  Yoshikazu Fujii ,  Shigeru Yamaguchi

    发明人： Atsuro Yoneda ,  Takahiro Tsumori ,  Yoshikazu Fujii ,  Shigeru Yamaguchi

    IPC分类号： C08F2/00

    CPC分类号： C08F20/06 ,  C08F290/062

    摘要： This invention concerns a (meth)acrylic acid type polymer, wherein the value S representing the quantity of an sulfur element introduced which is defined by the formula, S=(quantity of S contained in polymer)/(total quantity of S)×100, is not less than 35. This (meth) acrylic acid type polymer is a water-soluble polymer of a low molecular weight which has only a small impurity content, entails no precipitation of an impurity during the preservation at a low temperature, and excels in dispersibility, chelating ability, and an anti-gelling property.

    摘要翻译： 本发明涉及一种（甲基）丙烯酸型聚合物，其中代表由式S =（聚合物中所含的S的量）/（S的总量）×100所定义的引入的硫元素的量的值S， 不小于35.这种（甲基）丙烯酸类聚合物是低分子量的水溶性聚合物，其杂质含量小，在低温保存期间不会产生杂质沉淀，并且在 分散性，螯合能力和抗胶凝性。