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    Anti-cyanobacteria recombinant antibody polypeptide, gene thereof, and preparation method thereof
    5.
    发明授权
    Anti-cyanobacteria recombinant antibody polypeptide, gene thereof, and preparation method thereof 有权
    抗蓝细菌重组抗体多肽，其基因及其制备方法

    公开(公告)号：US09359429B2

    公开(公告)日：2016-06-07

    申请号：US14113188

    申请日：2012-03-27

    申请人： Xiaoqing Qiu

    发明人： Xiaoqing Qiu

    IPC分类号： A61K39/40 ,  A61K39/00 ,  A61K39/395 ,  C07K16/14 ,  C02F1/50 ,  C07K16/12 ,  C12N5/16

    CPC分类号： C07K16/14 ,  A61K39/00 ,  C02F1/50 ,  C07K16/12 ,  C12N5/163

    摘要： Provided are a hybridoma cell CGMCC No. 4783 that secretes a monoclonal antibody of an anti-cyanobacteria cell surface antigen, and the secreted monoclonal antibody thereof. Also provided are an anti-cyanobacteria recombinant antibody polypeptide, encoding gene, preparation method and use thereof. The anti-cyanobacteria recombinant antibody polypeptide is composed of an anti-cyanobacteria antibody mimetic polypeptide operably linearly connecting to the carboxyl terminal of an Escherichia coli polypeptide. The anti-cyanobacteria antibody mimetic polypeptide is a polypeptide with cyanobacteria identifying and binding capability designed based on an antigen binding fragment of the monoclonal antibody secreted by the CGMCC No. 4783 hybridoma cell. The anti-cyanobacteria recombinant antibody polypeptide directly form an ion channel on the cell membrane of a cyanobacteria to kill the cyanobacteria, targeted killing the cyanobacteria (prokaryote) without killing other beneficial eukaryotic cell algae.

    摘要翻译： 提供分泌抗蓝细菌细胞表面抗原的单克隆抗体及其分泌的单克隆抗体的杂交瘤细胞CGMCC No.4833。 还提供了抗蓝细菌重组抗体多肽，编码基因，其制备方法和用途。 抗蓝细菌重组抗体多肽由可操作地线性连接到大肠杆菌多肽的羧基末端的抗蓝细菌抗体模拟多肽组成。 抗蓝细菌抗体模拟多肽是具有蓝细菌鉴定和结合能力的多肽，其基于由CGMCC No.48383杂交瘤细胞分泌的单克隆抗体的抗原结合片段而设计。 抗蓝细菌重组抗体多肽直接在蓝细菌的细胞膜上形成离子通道以杀死蓝细菌，靶向杀死蓝细菌（原核细胞）而不杀死其他有益的真核细胞藻类。

    Antibiotic comprising an antibody mimetic, its preparation methods and uses thereof
    6.
    发明授权
    Antibiotic comprising an antibody mimetic, its preparation methods and uses thereof 有权
    抗生素包含抗体模拟物，其制备方法和用途

    公开(公告)号：US09073989B2

    公开(公告)日：2015-07-07

    申请号：US13013693

    申请日：2011-01-25

    申请人： Xiaoqing Qiu

    发明人： Xiaoqing Qiu

    IPC分类号： C07K14/24 ,  C07K14/245 ,  C07K16/12 ,  A61K47/48 ,  A61K39/00 ,  A61K38/00

    CPC分类号： C07K14/245 ,  A61K38/00 ,  A61K47/48484 ,  A61K47/48507 ,  A61K47/6829 ,  A61K47/6835 ,  A61K2039/505 ,  C07K16/1217 ,  C07K2317/565 ,  C07K2317/567 ,  C07K2317/569 ,  C07K2317/60 ,  C07K2319/01 ,  C07K2319/30

    摘要： The present invention belongs to field of biology and medicine, and especially relates to a novel antibiotic comprising an antibody mimetic antibody, its preparation methods and uses thereof. A novel antibiotic comprising a antibody mimetic covalently bonded to the carboxyl end of a colicin polypeptide or a channel-forming domain polypeptide of a colicin, wherein said colicin is selected from the group consisting of Colicin E1, Ia, Ib, A, B, N; wherein said antibody mimetic being yielded by fusing two complementarity determining regions (CDRs), VHCDR1 and VLCDR3 through a cognate framework region (VHFR2) of an immunoglobulin; wherein said the immunoglobulin specifically recognizes the bacterial porins. Its antibacterial ability is a thousandfold powerful than normal antibiotics. Due to its unique action mechanism, drug resistance resulted in mutation can hardly be acquired by pathogenic bacteria. And the antibiotic will not hurt normal human cells when it kills pathogenic bacteria. Therefore, it can be used for manufacturing antibacterial medicament of killing Neisseria meningitidis, vancomycin-resistant Enterococcus faecalis, methicillin-resistant Staphylococcus aureus, multidrug-resistance Pseudomonas aeruginosa or Mycobacterium tuberculosis.

    摘要翻译： 本发明属于生物学和医学领域，特别涉及包含抗体模拟抗体的新型抗生素，其制备方法和用途。 一种新型抗生素，其包含共价键合到大肠杆菌素多肽的羧基末端的抗体模拟物或者大肠菌素的通道形成结构域多肽，其中所述大肠菌素选自Colicin E1，Ia，Ib，A，B，N ; 其中通过将两个互补决定区（CDR），VHCDR1和VLCDR3通过免疫球蛋白的同源框架区（VHFR2）融合产生所述抗体模拟物; 其中所述免疫球蛋白特异性识别细菌孔蛋白。 其抗菌能力比正常抗生素强1000倍。 由于其独特的作用机制，导致突变的耐药性几乎不能被致病菌获得。 抗生素在杀死病原菌时不会伤害正常的人类细胞。 因此，可用于制造杀伤脑膜炎奈瑟菌，万古霉素耐药性粪肠球菌，耐甲氧西林金黄色葡萄球菌，多药耐药铜绿假单胞菌或结核分枝杆菌的抗菌药物。

    ANTI-CYANOBACTERIA RECOMBINANT ANTIBODY POLYPEPTIDE, GENE THEREOF, AND PREPARATION METHOD THEREOF
    7.
    发明申请
    ANTI-CYANOBACTERIA RECOMBINANT ANTIBODY POLYPEPTIDE, GENE THEREOF, AND PREPARATION METHOD THEREOF 有权
    抗细胞因子重组抗体多肽，其基因及其制备方法

    公开(公告)号：US20140170170A1

    公开(公告)日：2014-06-19

    申请号：US14113188

    申请日：2012-03-27

    申请人： Xiaoqing Qiu

    发明人： Xiaoqing Qiu

    IPC分类号： C07K16/14

    CPC分类号： C07K16/14 ,  A61K39/00 ,  C02F1/50 ,  C07K16/12 ,  C12N5/163

    摘要： Provided are a hybridoma cell CGMCC No. 4783 that secretes a monoclonal antibody of an anti-cyanobacteria cell surface antigen, and the secreted monoclonal antibody thereof. Also provided are an anti-cyanobacteria recombinant antibody poly-peptide, encoding gene, preparation method and use thereof. The anti-cyanobacteria recombinant antibody polypeptide is composed of an anti-cyanobacteria antibody mimetic polypeptide operably linearly connecting to the carboxyl terminal of an Escherichia coli polypeptide. The anti-cyanobacteria antibody mimetic polypeptide is a polypeptide with cyanobacteria identifying and binding cap-ability designed based on an antigen binding fragment of the monoclonal antibody secreted by the CGMCC No.4783 hybridoma cell. The anti-cyanobacteria recombinant antibody polypeptide directly form an ion channel on the cell membrane of a cyanobacteria to kill the cyanobacteria, targeted killing the cyanobacteria (prokaryote) without killing other beneficial eukaryotic cell algae.

    摘要翻译： 提供分泌抗蓝细菌细胞表面抗原的单克隆抗体及其分泌的单克隆抗体的杂交瘤细胞CGMCC No.4833。 还提供了抗蓝细菌重组抗体多肽，编码基因，其制备方法和用途。 抗蓝细菌重组抗体多肽由可操作地线性连接到大肠杆菌多肽的羧基末端的抗蓝细菌抗体模拟多肽组成。 抗蓝细菌抗体模拟多肽是根据由CGMCC No.4783杂交瘤细胞分泌的单克隆抗体的抗原结合片段设计的具有蓝细菌鉴定和结合帽能力的多肽。 抗蓝细菌重组抗体多肽直接在蓝细菌的细胞膜上形成离子通道以杀死蓝细菌，靶向杀死蓝细菌（原核细胞）而不杀死其他有益的真核细胞藻类。

    Antibiotic, its nucleotide sequence, methods of construction and uses thereof
    8.
    发明授权
    Antibiotic, its nucleotide sequence, methods of construction and uses thereof 有权
    抗生素，其核苷酸序列，构建方法和用途

    公开(公告)号：US08563503B2

    公开(公告)日：2013-10-22

    申请号：US13022512

    申请日：2011-02-07

    申请人： Xiaoqing Qiu

    发明人： Xiaoqing Qiu

    IPC分类号： A01N37/18 ,  A61K38/00 ,  A61P31/04 ,  C12P21/06 ,  C12N15/00 ,  C07K1/00 ,  C07K14/00 ,  C07K17/00 ,  C07H21/04

    CPC分类号： C07K14/245 ,  A61K38/164 ,  C07K14/31 ,  C07K2319/00

    摘要： The present invention belongs to field of biology and medicine, and especially relates to a novel antibiotic, its nucleotide sequence, methods of construction and uses thereof. A novel antibiotic, wherein the end of any peptide of the allosteric colicin is connected linearly to the end of peptide of the Staphylococcus aureus pheromone AgrD I, AgrD II, AgrD III, AgrD IV or Staphylococcus epidermidis pheromone. Wherein the allosteric colicin being yielded by artificially mutating the amino acid residues G11A, H22R, A26G, V31L and H40K in the peptide chain of wild type Colicin E1, Ia, Ib, A, B, N, or their ion channel-forming structural domain. In comparison with the traditional antibiotics, the novel antibiotics in the present invention are not likely to lead to drug resistance and cause hypersensitivity reaction.

    摘要翻译： 本发明属于生物医学领域，特别涉及新型抗生素，其核苷酸序列，构建方法及其用途。 一种新型抗生素，其中变构性大肠杆菌素的任何肽的末端与葡萄球菌信号素AgrD I，AgrD II，AgrD III，AgrD IV或表皮葡萄球菌信息素的肽的末端线性连接。 其中通过人造突变野生型Colicin E1，Ia，Ib，A，B，N的肽链中的氨基酸残基G11A，H22R，A26G，V31L和H40K产生的变构性大肠杆菌素或其离子通道形成结构域 。 与传统的抗生素相比，本发明的新型抗生素不可能导致耐药性并引起超敏反应。

    NOVEL ANTIBIOTIC COMPRISING AN ANTIBODY MIMETIC, ITS PREPARATION METHODS AND USES THEREOF
    9.
    发明申请
    NOVEL ANTIBIOTIC COMPRISING AN ANTIBODY MIMETIC, ITS PREPARATION METHODS AND USES THEREOF 有权
    包含抗体的新型抗生素，其制备方法及其用途

    公开(公告)号：US20120190826A1

    公开(公告)日：2012-07-26

    申请号：US13013693

    申请日：2011-01-25

    申请人： Xiaoqing Qiu

    发明人： Xiaoqing Qiu

    IPC分类号： C07K16/12 ,  C12N15/63 ,  C12P21/00 ,  C07H21/00

    CPC分类号： C07K14/245 ,  A61K38/00 ,  A61K47/48484 ,  A61K47/48507 ,  A61K47/6829 ,  A61K47/6835 ,  A61K2039/505 ,  C07K16/1217 ,  C07K2317/565 ,  C07K2317/567 ,  C07K2317/569 ,  C07K2317/60 ,  C07K2319/01 ,  C07K2319/30

    摘要： The present invention belongs to field of biology and medicine, and especially relates to a novel antibiotic comprising an antibody mimetic antibody, its preparation methods and uses thereof. A novel antibiotic comprising a antibody mimetic covalently bonded to the carboxyl end of a colicin polypeptide or a channel-forming domain polypeptide of a colicin, wherein said colicin is selected from the group consisting of Colicin E1, Ia, Ib, A, B, N; wherein said antibody mimetic being yielded by fusing two complementarity determining regions (CDRs), VHCDR1 and VLCDR3 through a cognate framework region (VHFR2) of an immunoglobulin; wherein said the immunoglobulin specifically recognizes the bacterial porins. Its antibacterial ability is a thousandfold powerful than normal antibiotics. Due to its unique action mechanism, drug resistance resulted in mutation can hardly be acquired by pathogenic bacteria. And the antibiotic will not hurt normal human cells when it kills pathogenic bacteria. Therefore, it can be used for manufacturing antibacterial medicament of killing Neisseria meningitidis, vancomycin-resistant Enterococcus faecalis, methicillin-resistant Staphylococcus aureus, multidrug-resistance Pseudomonas aeruginosa or Mycobacterium tuberculosis.

    摘要翻译： 本发明属于生物学和医学领域，特别涉及包含抗体模拟抗体的新型抗生素，其制备方法和用途。 一种新型抗生素，其包含共价键合到大肠杆菌素多肽的羧基末端的抗体模拟物或者大肠菌素的通道形成结构域多肽，其中所述大肠菌素选自Colicin E1，Ia，Ib，A，B，N ; 其中通过将两个互补决定区（CDR），VHCDR1和VLCDR3通过免疫球蛋白的同源框架区（VHFR2）融合产生所述抗体模拟物; 其中所述免疫球蛋白特异性识别细菌孔蛋白。 其抗菌能力比正常抗生素强1000倍。 由于其独特的作用机制，导致突变的耐药性几乎不能被致病菌获得。 抗生素在杀死病原菌时不会伤害正常的人类细胞。 因此，可用于制造杀伤脑膜炎奈瑟菌，万古霉素耐药性粪肠球菌，耐甲氧西林金黄色葡萄球菌，多药耐药铜绿假单胞菌或结核分枝杆菌的抗菌药物。

    Engineered antibiotic peptides and the preparation thereof
    10.
    发明授权
    Engineered antibiotic peptides and the preparation thereof 失效
    工程抗生素肽及其制备方法

    公开(公告)号：US06942993B2

    公开(公告)日：2005-09-13

    申请号：US10241564

    申请日：2002-09-09

    申请人： Xiaoqing Qiu

    发明人： Xiaoqing Qiu

    IPC分类号： C07K14/245 ,  C07K14/31 ,  C07K14/315 ,  C12P21/02 ,  A61K38/16 ,  C07K14/195

    CPC分类号： C07K14/315 ,  C07K14/245 ,  C07K14/31 ,  C07K14/3156

    摘要： Engineered antibiotic peptides and the preparation thereof are provided. The engineered antibiotic peptides, designated pheromonicins, are prepared by linking different bacteriocins or their functional domains with bacterial pheromones. Also provided are methods for treating bacterial infections by use of the antibiotic peptides.

    摘要翻译： 提供了工程化抗生素肽及其制备方法。 通过将不同的细菌素或其功能结构域与细菌信息素连接来制备工程化的抗生素肽，称为皮质激素。 还提供了通过使用抗生素肽治疗细菌感染的方法。