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公开(公告)号：US4561152A
公开(公告)日：1985-12-31
申请号：US572664
申请日：1984-01-20
申请人： Kiwamu Niimi , Kenji Kinoshita
发明人： Kiwamu Niimi , Kenji Kinoshita
CPC分类号： D01H5/82
摘要： The present invention provides a draft roll arrangement in a spinning frame in which the apron bottom roll is longitudinally divided into two sections at a location along its length selected to be within a range for which torsion of the apron bottom roll does not pose a problem in spinning, and in which the respective bottom rolls are operatively connected to one another in such a manner that one of the ends of the back and front bottom rolls and one apron bottom roll section are connected to a driving source and the drive for the other apron bottom roll section is derived through the back and front bottom rolls. In a preferred embodiment, the respective apron bottom roll sections are associated with rotational angle sensors whose output rotational angle signals are supplied to a control unit. When the difference in the rotational angle has exceeded a preset value, an output signal indicating such state is issued from the control unit.
摘要翻译： 本发明提供了一种纺纱机中的牵伸辊装置，其中围裙底辊在沿着其长度的一个位置上被纵向分成两部分，所述位置被选择在一个范围内，该范围使围裙底辊的扭转不会引起问题 旋转，并且其中相应的底辊可操作地彼此连接，使得后底部和前部底部的端部中的一个辊和一个皮圈底部辊部分连接到驱动源并且用于另一个皮圈的驱动 底辊部分通过后底辊和前底辊获得。 在优选实施例中，相应的围裙底辊部分与其输出旋转角度信号被提供给控制单元的旋转角传感器相关联。 当旋转角度的差超过预设值时，从控制单元发出指示这种状态的输出信号。
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2.发明申请Method of Elongating Dna Chain, Method of Amplifying Dna Chain, and Microarray for Dna Chain Elongation 失效延长Dna链的方法，扩增Dna链的方法和Dna链伸长的微阵列公开(公告)号：US20080032348A1
公开(公告)日：2008-02-07
申请号：US11792512
申请日：2005-11-29
申请人： Kenji Kinoshita
发明人： Kenji Kinoshita
CPC分类号： C12Q1/6834 , B01J2219/00585 , B01J2219/00596 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00626 , B01J2219/00635 , B01J2219/00637 , B01J2219/00659 , B01J2219/00722 , C12Q1/686 , C12Q2531/113 , C12Q2527/125 , C12Q2527/107 , C12Q2565/519
摘要： Primers for DNA elongation are immobilized onto a substrate having on the surface thereof a polymer substance which contains a first unit having a group derived from a phosphoester composing the hydrophilic section of a phospholipid, and a second unit having an activated ester group, and DNA elongation reaction is allowed to proceed in a reaction system having introduced therein a sample which contains DNA fragments having desired sequences and nucleotide monomers, by heating the reaction system up to a temperature causing thermal unfolding of the DNA chains, and then by cooling the reaction system down to a temperature for annealing.
摘要翻译： 用于DNA延伸的引物固定在其表面上的基材上，该聚合物物质含有具有源自构成磷脂的亲水部分的磷酸酯的基团的第一单元和具有活化酯基的第二单元，以及DNA伸长 在其中引入反应体系的反应体系中，通过将反应体系加热至导致DNA链的热解折叠的温度，然后将反应体系冷却下来，在其中引入含有具有所需序列的DNA片段的样品和核苷酸单体的反应体系 达到退火温度。
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3.发明授权公开(公告)号：US06341063B2
公开(公告)日：2002-01-22
申请号：US09770404
申请日：2001-01-29
申请人： Kenji Kinoshita , Hideki Ishihara , Akira Kurimoto , Haruki Matsuzaki , Ryoichi Yamamoto , Akihiro Yoshida , Ryouta Nakamura , Toshiki Kobayashi , Hajime Katsuro
发明人： Kenji Kinoshita , Hideki Ishihara , Akira Kurimoto , Haruki Matsuzaki , Ryoichi Yamamoto , Akihiro Yoshida , Ryouta Nakamura , Toshiki Kobayashi , Hajime Katsuro
IPC分类号： H05K720
CPC分类号： H05K5/0039
摘要： A casing of an ECU is formed within an air cleaner of an intake module for an internal combustion engine. The casing faces an intake air passage, and formed in a pocket, so that the casing is easily formed and the water and dust proof performances thereof are improved. A printed-circuit board to which a metal plate is fixed is inserted into the casing along slits formed on an inner wall of the casing. The slits pinch the printed-circuit board and the metal plate at the rear end area thereof. Thus, anti-stress and anti-vibration performances of the printed-circuit board are improved. The metal plate including convexes and concaved contacting portions is easily formed by pressing an aluminum or steel plate. Thus, the metal plate is formed with low cost. Further, the metal plate appropriately includes holes, thereby promoting the heat radiation and reducing the weight thereof.
摘要翻译： ECU的外壳形成在用于内燃机的进气模块的空气净化器内。 壳体面向进气通道，并且形成在口袋中，使得壳体容易地形成，并且其防水和防尘性能得到改善。 固定有金属板的印刷电路板沿着形成在壳体的内壁上的狭缝插入到壳体中。 狭缝在其后端区域夹紧印刷电路板和金属板。 因此，改善了印刷电路板的抗应力和抗振动性能。 包括凸形和凹形接触部分的金属板可以通过压制铝或钢板而容易地形成。 因此，以低成本形成金属板。 此外，金属板适当地包括孔，从而促进热辐射并减轻其重量。
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公开(公告)号：US5631585A
公开(公告)日：1997-05-20
申请号：US512649
申请日：1995-08-08
IPC分类号： G01P3/481 , G01D5/244 , G01D5/245 , G01R1/28 , G01R19/165 , H03K5/08 , H03K5/1252
CPC分类号： H03K5/088
摘要： A wave-shaping circuit comprises a dynamic hysteresis generating circuit for generating a dynamic hysteresis voltage having a certain crest value and whose signal level attenuates at a speed proportional to a number of engine revolutions to mask an ignition noise superimposed on an input signal, and a threshold generating circuit for generating a threshold voltage whose signal level increases/decreases in proportion to the number of engine revolutions to mask an interference noise superimposed on the input signal and to reliably detect a minimum level of the input signal. Either the dynamic hysteresis voltage or the threshold voltage, whichever is higher, is selectively output as a comparison reference signal through a high-level selecting circuit.
摘要翻译： 波形整形电路包括用于产生具有一定峰值的动态滞后电压的动态滞后产生电路，其信号电平以与发动机转数成比例的速度衰减，以掩盖叠加在输入信号上的点火噪声，以及 阈值产生电路，用于产生其信号电平与发动机转数成比例地增减的阈值电压，以掩盖叠加在输入信号上的干扰噪声并可靠地检测输入信号的最小电平。 通过高电平选择电路有选择地输出动态滞后电压或阈值电压（以较高者为准）作为比较参考信号输出。
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公开(公告)号：US07968290B2
公开(公告)日：2011-06-28
申请号：US11920559
申请日：2006-05-16
CPC分类号： C12N15/1096 , C12Q1/6834 , C12Q2565/537 , C12Q2527/107
摘要： A method of detecting a gene including immobilizing a primer for DNA elongation onto an insoluble carrier having on the surface thereof a polymer substance containing a first unit having a phosphorylcholine group and a second unit having a carboxylic acid-derived group having an electron-attractive substituent bound to a carbonyl group; annealing the template DNA fragments or RNA fragments with the primer for DNA elongation, so as to elongate the DNA primer while incorporating therein an enzyme, thereby allowing coloration of a chromogenic reagent by its enzymatic action; and judging whether the DNA fragments or RNA fragments of the gene presents or not, based on the degree of coloration.
摘要翻译： 一种检测基因的方法，包括将DNA延伸引物固定在具有表面的不溶性载体上，所述不溶性载体具有含有具有磷酸胆碱基团的第一单元的聚合物物质和具有吸电子取代基的羧酸衍生基团的第二单元 与羰基结合; 用引物退火模板DNA片段或RNA片段以进行DNA延伸，以便在引入酶的同时延长DNA引物，从而通过其酶作用使着色试剂着色; 并基于着色程度来判断该基因的DNA片段或RNA片段是否存在。
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公开(公告)号：US20090137406A1
公开(公告)日：2009-05-28
申请号：US12227904
申请日：2007-06-01
申请人： Kenji Kinoshita , Kanehisa Yokoyama , Kentaro Fujimoto , Toru Yakabe , Shin Saito , Kazuhiko Fujiwara
发明人： Kenji Kinoshita , Kanehisa Yokoyama , Kentaro Fujimoto , Toru Yakabe , Shin Saito , Kazuhiko Fujiwara
CPC分类号： C12Q1/6837
摘要： The present invention provides an RNA detection method detecting, from a reaction system containing a target sample, a target RNA chain originated from the target sample, using a surface having on the surface thereof a polymer substance which contains a first unit having a group derived from a phosphate ester composing the hydrophilic portion of a phospholipid and a second unit having a carboxylic acid derivative group composed of an electron-attractive substitutional group bound to a carbonyl group, while being provided with at least one reaction space, the reaction space having an immobilized nucleic acid primer immobilized therein.
摘要翻译： 本发明提供一种RNA检测方法，其从含有目标样品的反应体系中检测源自目标样品的靶RNA链，其表面上具有含有第一单元的表面，该第一单元含有来自 构成磷脂的亲水部分的磷酸酯和具有由与羰基结合的吸电子取代基组成的羧酸衍生物基团的第二单元，同时具有至少一个反应空间，所述反应空间具有固定化的 固定在其中的核酸引物。
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公开(公告)号：US06788534B2
公开(公告)日：2004-09-07
申请号：US10351314
申请日：2003-01-27
申请人： Kenji Kinoshita , Satoru Umemoto
发明人： Kenji Kinoshita , Satoru Umemoto
IPC分类号： H05K720
CPC分类号： B60R16/0239 , H05K7/20163 , H05K7/20854
摘要： An intake module has an electronic control unit (ECU) housing portion that houses an ECU. The ECU housing portion is provided in a manner that a lateral direction of the ECU housing portion is approximately coincident with a direction of an intake air passage that is located upstream from the ECU housing portion. Intake air is blown to an air blown area that is located on one side of the ECU housing portion. Heat-producing components and low heat-resistant components are mounted in the air blown area and in an area away from the air blown area, respectively. A plurality of heat-radiating fins is arranged in the air blown area along with a stream of the intake air.
摘要翻译： 进气模块具有容纳ECU的电子控制单元（ECU）壳体部分。 ECU壳体部分设置成使得ECU壳体部分的横向方向与位于ECU壳体部分上游的进气通道的方向近似一致。 进气被吹送到位于ECU壳体部分一侧的吹风区域。 发热部件和低耐热部件分别安装在吹风区域和远离吹风区域的区域中。 多个散热翅片与进气的流一起布置在吹风区域中。
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8.发明授权Method of synthesizing cDNA and method of synthesizing RNA chains, and nucleotide-immobilized carrier 有权合成cDNA的方法和合成RNA链的方法以及核苷酸固定载体公开(公告)号：US08822670B2
公开(公告)日：2014-09-02
申请号：US11990641
申请日：2006-08-09
IPC分类号： C07H21/00
CPC分类号： C12P19/34 , C12N15/1096 , C12Q1/686 , C12Q2521/107 , C12Q2565/537
摘要： A method of synthesizing a cDNA chain using an insoluble carrier having on the surface thereof a polymer substance containing a first unit having a group derived from a phosphoric ester composing the hydrophilic portion of phospholipid, and a second unit having a group derived from carboxylic acid having an electron-attractive substituent bound to a carbonyl group, which includes immobilizing a polynucleotide for DNA elongation; bringing a solution containing an RNA fragment, nucleotide monomers, and a reverse transcriptase or an enzyme having polymerase activity into contact with the surface of the insoluble carrier; and allowing the polynucleotide for DNA elongation immobilized on the surface of the carrier to elongate using the RNA fragment contained in the solution as a template, to thereby form a single-strand cDNA.
摘要翻译： 一种使用不溶性载体合成cDNA链的方法，所述不溶性载体在其表面上具有含有源自构成磷脂的亲水部分的磷酸酯的基团的第一单元的聚合物物质和具有衍生自羧酸的基团的第二单元， 与羰基结合的电子吸引取代基，其包括固定多核苷酸用于DNA延伸; 将含有RNA片段，核苷酸单体和逆转录酶或具有聚合酶活性的酶的溶液与不溶性载体的表面接触; 并且使用溶液中包含的RNA片段作为模板，使固定在载体表面上的DNA延伸的多核苷酸伸长，从而形成单链cDNA。
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公开(公告)号：US08088580B2
公开(公告)日：2012-01-03
申请号：US12227904
申请日：2007-06-01
申请人： Kenji Kinoshita , Kanehisa Yokoyama , Kentaro Fujimoto , Toru Yakabe , Shin Saito , Kazuhiko Fujiwara
发明人： Kenji Kinoshita , Kanehisa Yokoyama , Kentaro Fujimoto , Toru Yakabe , Shin Saito , Kazuhiko Fujiwara
CPC分类号： C12Q1/6837
摘要： The present invention provides an RNA detection method detecting, from a reaction system containing a target sample, a target RNA chain originated from the target sample, using a surface having on the surface thereof a polymer substance which contains a first unit having a group derived from a phosphate ester composing the hydrophilic portion of a phospholipid and a second unit having a carboxylic acid derivative group composed of an electron-attractive substitutional group bound to a carbonyl group, while being provided with at least one reaction space, the reaction space having an immobilized nucleic acid primer immobilized therein.
摘要翻译： 本发明提供一种RNA检测方法，其从含有目标样品的反应体系中检测源自目标样品的靶RNA链，其表面上具有含有第一单元的表面，该第一单元含有来自 构成磷脂的亲水部分的磷酸酯和具有由与羰基结合的吸电子取代基组成的羧酸衍生物基团的第二单元，同时具有至少一个反应空间，所述反应空间具有固定化的 固定在其中的核酸引物。
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公开(公告)号：US20090042190A1
公开(公告)日：2009-02-12
申请号：US11920559
申请日：2006-05-16
IPC分类号： C12Q1/68
CPC分类号： C12N15/1096 , C12Q1/6834 , C12Q2565/537 , C12Q2527/107
摘要： A method of detecting a gene including immobilizing a primer for DNA elongation onto an insoluble carrier having on the surface thereof a polymer substance containing a first unit having a phosphorylcholine group and a second unit having a carboxylic acid-derived group having an electron-attractive substituent bound to a carbonyl group; annealing the template DNA fragments or RNA fragments with the primer for DNA elongation, so as to elongate the DNA primer while incorporating therein an enzyme, thereby allowing coloration of a chromogenic reagent by its enzymatic action; and judging whether the DNA fragments or RNA fragments of the gene presents or not, based on the degree of coloration.
摘要翻译： 一种检测基因的方法，包括将DNA延伸引物固定在具有表面的不溶性载体上，所述不溶性载体具有含有具有磷酸胆碱基团的第一单元的聚合物物质和具有吸电子取代基的羧酸衍生基团的第二单元 与羰基结合; 用引物退火模板DNA片段或RNA片段以进行DNA延伸，以便在引入酶的同时延长DNA引物，从而通过其酶作用使着色试剂着色; 并基于着色程度来判断该基因的DNA片段或RNA片段是否存在。
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