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公开(公告)号：US08383369B2
公开(公告)日：2013-02-26
申请号：US12561221
申请日：2009-09-16
申请人： Kenneth Mark Maxham , Jon Sorenson , John Eid , Patrick Marks , Kevin Travers , Donald Gray , Robin Emig , Mark Chaisson , Benjamin Flusberg
发明人： Kenneth Mark Maxham , Jon Sorenson , John Eid , Patrick Marks , Kevin Travers , Donald Gray , Robin Emig , Mark Chaisson , Benjamin Flusberg
CPC分类号： G01N21/6452 , C12Q1/6869 , G01N21/6428 , G01N2201/0635 , Y10T436/143333 , C12Q2525/119 , C12Q2521/125 , C12Q2537/149
摘要： Methods, devices, and systems for performing intermittent detection during analytical reactions are provided. Such methods facilitate collection of reaction data from disparate reaction times. Further, such methods are useful for reducing photo-induced damage of one or more reactants in an illuminated analytical reaction at a given reaction time. In preferred embodiments, the reaction mixture is subjected to at least one illuminated and non-illuminated period and allowed to proceed such that the time in which the reaction mixture is illuminated is less than a photo-induced damage threshold period.
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公开(公告)号：US20100075327A1
公开(公告)日：2010-03-25
申请号：US12561221
申请日：2009-09-16
申请人： Kenneth Mark Maxham , Jon Sorenson , John Eid , Patrick Marks , Kevin Travers , Donald Gray , Robin Emig , Mark Chaisson , Benjamin Flusberg
发明人： Kenneth Mark Maxham , Jon Sorenson , John Eid , Patrick Marks , Kevin Travers , Donald Gray , Robin Emig , Mark Chaisson , Benjamin Flusberg
CPC分类号： G01N21/6452 , C12Q1/6869 , G01N21/6428 , G01N2201/0635 , Y10T436/143333 , C12Q2525/119 , C12Q2521/125 , C12Q2537/149
摘要： Methods, devices, and systems for performing intermittent detection during analytical reactions are provided. Such methods facilitate collection of reaction data from disparate reaction times. Further, such methods are useful for reducing photo-induced damage of one or more reactants in an illuminated analytical reaction at a given reaction time. In preferred embodiments, the reaction mixture is subjected to at least one illuminated and non-illuminated period and allowed to proceed such that the time in which the reaction mixture is illuminated is less than a photo-induced damage threshold period.
摘要翻译： 提供了在分析反应期间执行间歇检测的方法，装置和系统。 这种方法有助于从不同的反应时间收集反应数据。 此外，这样的方法可用于在给定的反应时间下降低照明分析反应中一种或多种反应物的光诱导损伤。 在优选的实施方案中，将反应混合物经受至少一个照明和非照射期，并允许进行，使得反应混合物被照射的时间小于光诱导的损伤阈值期。
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公开(公告)号：US20100075309A1
公开(公告)日：2010-03-25
申请号：US12413226
申请日：2009-03-27
申请人： Kenneth Mark Maxham , Jon Sorenson , John Eid , Patrick Marks , Kevin Travers
发明人： Kenneth Mark Maxham , Jon Sorenson , John Eid , Patrick Marks , Kevin Travers
IPC分类号： C12Q1/68 , G01N21/75 , C12Q1/48 , C12Q1/00 , C12Q1/44 , G01N33/48 , G01N21/76 , C12P19/34 , G06F19/00
CPC分类号： C12Q1/6869 , G01N2021/7786 , Y10T436/143333 , C12Q2525/301 , C12Q2533/101 , C12Q2537/149 , C12Q2565/631 , C12Q2525/307 , C12Q2525/191
摘要： Methods, devices, and systems for performing intermittent detection during analytical reactions are provided. Such methods facilitate collection of reaction data from disparate reaction times. Further, such methods are useful for reducing photo-induced damage of one or more reactants in an illuminated analytical reaction at a given reaction time. In preferred embodiments, the reaction mixture is subjected to at least one illuminated and non-illuminated period and allowed to proceed such that the time in which the reaction mixture is illuminated is less than a photo-induced damage threshold period.
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公开(公告)号：US08143030B2
公开(公告)日：2012-03-27
申请号：US12413226
申请日：2009-03-27
申请人： Kenneth Mark Maxham , Jon Sorenson , John Eid , Patrick Marks , Kevin Travers
发明人： Kenneth Mark Maxham , Jon Sorenson , John Eid , Patrick Marks , Kevin Travers
CPC分类号： C12Q1/6869 , G01N2021/7786 , Y10T436/143333 , C12Q2525/301 , C12Q2533/101 , C12Q2537/149 , C12Q2565/631 , C12Q2525/307 , C12Q2525/191
摘要： Methods, devices, and systems for performing intermittent detection during analytical reactions are provided. Such methods facilitate collection of reaction data from disparate reaction times. Further, such methods are useful for reducing photo-induced damage of one or more reactants in an illuminated analytical reaction at a given reaction time. In preferred embodiments, the reaction mixture is subjected to at least one illuminated and non-illuminated period and allowed to proceed such that the time in which the reaction mixture is illuminated is less than a photo-induced damage threshold period.
摘要翻译： 提供了在分析反应期间执行间歇检测的方法，装置和系统。 这种方法有助于从不同的反应时间收集反应数据。 此外，这样的方法可用于在给定的反应时间下降低照明分析反应中的一种或多种反应物的光致损伤。 在优选的实施方案中，将反应混合物经受至少一个照明和非照射期，并允许进行，使得反应混合物被照射的时间小于光诱导的损伤阈值期。
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公开(公告)号：US20100009872A1
公开(公告)日：2010-01-14
申请号：US12384097
申请日：2009-03-30
申请人： John Eid , Stephen Turner , Ravi Dalal , Benjamin Flusberg , Jonas Korlach , Steven Lin , Adrian Fehr , Fred Christians , Robin Emig
发明人： John Eid , Stephen Turner , Ravi Dalal , Benjamin Flusberg , Jonas Korlach , Steven Lin , Adrian Fehr , Fred Christians , Robin Emig
CPC分类号： B01J19/0046 , B01J2219/00596 , C12N2310/50 , C12N2310/51 , C12Q1/6869 , C12Q2535/122 , C40B40/06 , G01N33/54313 , G01N33/54366
摘要： Methods, compositions and arrays for non-random loading of single analyte molecules into array structures are provided.
摘要翻译： 提供了将单一分析物分子非随机加载到阵列结构中的方法，组合物和阵列。
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公开(公告)号：US08906831B2
公开(公告)日：2014-12-09
申请号：US12384097
申请日：2009-03-30
申请人： John Eid , Stephen Turner , Ravi Dalal , Benjamin Flusberg , Jonas Korlach , Steven Lin , Adrian Fehr , Fred Christians , Robin Emig , Jeremy Gray , Robert Sebra , Lei Sun , Stephen Dudek
发明人： John Eid , Stephen Turner , Ravi Dalal , Benjamin Flusberg , Jonas Korlach , Steven Lin , Adrian Fehr , Fred Christians , Robin Emig , Jeremy Gray , Robert Sebra , Lei Sun , Stephen Dudek
CPC分类号： B01J19/0046 , B01J2219/00596 , C12N2310/50 , C12N2310/51 , C12Q1/6869 , C12Q2535/122 , C40B40/06 , G01N33/54313 , G01N33/54366
摘要： Methods for non-random loading of single analyte molecules into array structures are provided. The methods allow for distribution of a population of target molecules into a plurality of size confined regions such as wells. Sizing moieties are linked to individual target molecules. The sizing moieties are of sufficient size, relative to the size-confined reaction or observation regions, such that only a selected number of sizing moieties will fit into the size confined regions. The confined regions and the sizing moieties or target molecules comprise a selected charge that allow for controlling the loading of the sizing moities.
摘要翻译： 提供了将单一分析物分子非随机加载到阵列结构中的方法。 所述方法允许将目标分子群体分配到多个尺寸的限制区域例如孔中。 上限部分与单独的靶分子连接。 尺寸部分相对于尺寸限制的反应或观察区域具有足够的尺寸，使得只有选定数量的施胶部分将适合尺寸限制区域。 限制区域和施胶部分或靶分子包括允许控制施胶剂的载荷的选定的电荷。
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公开(公告)号：US20090208957A1
公开(公告)日：2009-08-20
申请号：US12315626
申请日：2008-12-03
申请人： Jonas Korlach , Daniel Roitman , John Eid , Geoff Otto , Paul Hardenbol , Benjamin Flusberg
发明人： Jonas Korlach , Daniel Roitman , John Eid , Geoff Otto , Paul Hardenbol , Benjamin Flusberg
CPC分类号： G01N33/54373 , C12Q1/6874 , G01N33/542 , G01N33/54313 , G01N33/573 , G01N33/58 , G01N33/585 , C12Q2565/607 , C12Q2565/531 , C12Q2563/143 , C12Q2563/137
摘要： Systems and methods of enhancing fluorescent labeling strategies as well as systems and methods of using non-fluorescent and/or non-optic labeling strategies, e.g., as with single molecule sequencing using ZMWs, are described.
摘要翻译： 描述了增强荧光标记策略的系统和方法以及使用非荧光和/或非光学标记策略的系统和方法，例如与使用ZMW的单分子测序一样。
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公开(公告)号：US20080176241A1
公开(公告)日：2008-07-24
申请号：US11981627
申请日：2007-10-31
申请人： John Eid , Devon Murphy , Geoffrey Otto , Stephen Turner
发明人： John Eid , Devon Murphy , Geoffrey Otto , Stephen Turner
CPC分类号： A61K38/446 , A61K38/063 , A61K38/443 , G01N33/582
摘要： Compositions, devices, systems and methods for reducing and/or preventing photodamage of one or more reactants in illuminated analytical reactions by one or more of incorporating photodamage mitigating agents within the reaction mixture and/or interrogating different observation regions of the reaction mixture for a period that is less than a photodamage threshold period.
摘要翻译： 用于通过一种或多种在反应混合物中掺入光损伤缓和剂和/或在反应混合物中询问不同观察区域一段时间来减少和/或防止一种或多种反应物在照明分析反应中的光损伤的组合物，装置，系统和方法 这小于光损伤阈值期。
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公开(公告)号：US20070161017A1
公开(公告)日：2007-07-12
申请号：US11566153
申请日：2006-12-01
申请人： John Eid , Devon Murphy , Geoffrey Otto , Stephen Turner
发明人： John Eid , Devon Murphy , Geoffrey Otto , Stephen Turner
CPC分类号： A61K38/446 , A61K38/063 , A61K38/443 , G01N33/582
摘要： Compositions, devices, systems and methods for reducing and/or preventing photodamage of one or more reactants in illuminated analytical reactions by one or more of incorporating photodamage mitigating agents within the reaction mixture and/or interrogating different observation regions of the reaction mixture for a period that is less than a photodamage threshold period.
摘要翻译： 用于通过一种或多种在反应混合物中掺入光损伤缓和剂和/或在反应混合物中询问不同观察区域一段时间来减少和/或防止一种或多种反应物在照明分析反应中的光损伤的组合物，装置，系统和方法 这小于光损伤阈值期。
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公开(公告)号：US20070128133A1
公开(公告)日：2007-06-07
申请号：US11293040
申请日：2005-12-02
申请人： John Eid , Devon Murphy , Geoffrey Otto , Stephen Turner
发明人： John Eid , Devon Murphy , Geoffrey Otto , Stephen Turner
CPC分类号： A61K38/446 , A61K38/063 , A61K38/443 , G01N33/582
摘要： Compositions, devices, systems and methods for reducing and/or preventing photodamage of one or more reactants in illuminated analytical reactions by one or more of incorporating photodamage mitigating agents within the reaction mixture and/or interrogating different observation regions of the reaction mixture for a period that is less than a photodamage threshold period.
摘要翻译： 用于通过一种或多种在反应混合物中掺入光损伤缓和剂和/或在反应混合物中询问不同观察区域一段时间来减少和/或防止一种或多种反应物在照明分析反应中的光损伤的组合物，装置，系统和方法 这小于光损伤阈值期。
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