Biodegradable targetable microparticle delivery system
    1.
    发明申请
    Biodegradable targetable microparticle delivery system 审中-公开
    可生物降解的可靶向微粒输送系统

    公开(公告)号:US20050163745A1

    公开(公告)日:2005-07-28

    申请号:US10620686

    申请日:2003-07-17

    摘要: Copolymers designed for use as particulate carriers containing functionalizable amino acid subunits for coupling with targeting ligands are described. The copolymers are polyesters composed of α-hydroxy acid subunits such as D,L-lactide and pseudo-α-amino acid subunits which may be derived from serine or terpolymers of D,L-lactide and glycolide and pseudo-α-amino acid subunits which may be derived from serine. Stable vaccine preparations useful as delayed release formulations containing antigen or antigens and adjuvants encapsulated within or physically mixed with polymeric microparticles are described. The particulate carriers are useful for delivering agents to the immune system of a subject by mucosal or parenteral routes to produce immune responses, including antibody and protective responses.

    摘要翻译: 描述了设计用作颗粒载体的共聚物,其含有用于与靶配体偶联的可官能化氨基酸亚单位。 共聚物是由α-羟基酸亚基如D,L-丙交酯和拟α-氨基酸亚基组成的聚酯,其可衍生自D,L-丙交酯和乙交酯的丝氨酸或三元共聚物和假α-氨基酸亚基 其可以来自丝氨酸。 描述了可用作包含包封在聚合物微粒内或与聚合物微粒物理混合的抗原或抗原和佐剂的延迟释放制剂的稳定的疫苗制剂。 颗粒载体可用于通过粘膜或肠胃外途径将药剂递送至受试者的免疫系统,以产生免疫应答,包括抗体和保护性反应。

    Purification of a pertussis outer membrane protein

    公开(公告)号:US06444211B1

    公开(公告)日:2002-09-03

    申请号:US09327527

    申请日:1999-06-08

    IPC分类号: A61K3910

    摘要: Pertactin (formerly 69 kDa protein) is recovered in stable biologically pure form having no detectable adenylate cyclase activity from fermentation broth from the fermentation of Bordetella pertussis as well as from the cells. The broth is processed to selectively remove pertussis toxin (PT) and filamentous haemagglutinin (FHA), the pertactin is precipitated by ammonium sulphate and the precipitate is dissolved in buffer at pH 6.0 to 8.5, the solution then is passed through hydroxyapatite and ion-exchange chromatograph columns before final ultrafiltration. Cells are extracted with urea and the extract ultrafiltered and diafiltered. The pertactin is precipitated from the extract and the precipitate processed as above. In a variation, the broth is contacted with ammonium sulphate to precipitate pertactin, PT and FHA, the precipitate is dissolved and the PT and FHA selectively removed, before the solution is passed to the chromatograph columns.

    Haemophilus outer membrane protein
    3.
    发明授权
    Haemophilus outer membrane protein 失效
    嗜血杆菌外膜蛋白

    公开(公告)号:US06264954B1

    公开(公告)日:2001-07-24

    申请号:US08942046

    申请日:1997-10-01

    IPC分类号: A61K39102

    摘要: Purified and isolated nucleic acid from specific strains of Haemophilus influenzae is provided which encodes at least a portion of the D15 outer membrane protein of Haemophilus. The nucleic acid is used to produce peptides, polypeptides and proteins free of contaminant associated with Haemophilus for purposes of diagnosis and medical treatment. Furthermore, the nucleic acid may be used in the diagnosis of Haemophilus infection. Antisera obtained following immunization with the nucleic acid D15 outer membrane protein or peptides also may be used for the purpose of diagnosis and medical treatment.

    摘要翻译: 提供了来自流感嗜血杆菌的特异性菌株的纯化和分离的核酸,其编码嗜血杆菌D15外膜蛋白的至少一部分。 为了诊断和治疗的目的,该核酸用于产生不含与嗜血杆菌相关的污染物的肽,多肽和蛋白质。 此外,核酸可用于诊断嗜血杆菌感染。 用核酸D15外膜蛋白或肽免疫后获得的抗血清也可用于诊断和治疗的目的。

    Outer membrane protein P1 and peptides of Haemophilus influenzae type B
    10.
    发明授权
    Outer membrane protein P1 and peptides of Haemophilus influenzae type B 失效
    外膜蛋白P1和B型流感嗜血杆菌肽

    公开(公告)号:US5985288A

    公开(公告)日:1999-11-16

    申请号:US472172

    申请日:1995-06-07

    CPC分类号: C07K14/285 A61K39/00

    摘要: The gene for outer membrane protein P1 of Haemophilus influenzae b is expressed in E. coli. Methods for expression and demonstration of the immunogenicity of recombinant P1 and portions thereof are disclosed, along with an improved method for the purification of P1. The nucleotide sequence of the P1 gene and the derived amino acid sequence of the P1 protein of Haemophilus influenzae type b are disclosed and the methods used to determine the same. Also disclosed are the methods used to clone and express the P1 gene as well as the purification protocol for the P1 gene products (recombinant P1 and P1 fusion proteins). Fourteen peptides are synthesized corresponding to specific sequences of the mature P1 protein. The use of the P1 protein as n immunogens for immunization against the disease caused by Haemophilus influenzae type b and the use of the protein as a carrier for conjugation with an oligosaccharide derived from Haemophilus to generate a potentially efficacious vaccine against the disease, are described. Also disclosed is the use of P1 peptide-conjugates as immunizing agents to elicit anti-Haemophilus influenzae type b antibodies.

    摘要翻译: 流感嗜血杆菌b的外膜蛋白P1的基因在大肠杆菌中表达。 公开了用于表达和证明重组P1及其部分的免疫原性的方法以及用于纯化P1的改进方法。 公开了P1型基因的核苷酸序列和B型流感嗜血杆菌的P1蛋白的衍生氨基酸序列,并且用于确定它们的方法。 还公开了用于克隆和表达P1基因的方法以及P1基因产物(重组P1和P1融合蛋白)的纯化方案。 对应于成熟P1蛋白的特异性序列合成14个肽。 描述了使用P1蛋白作为免疫原免疫针对由流感嗜血杆菌b型引起的疾病的免疫,以及使用蛋白质作为与源自嗜血杆菌的寡糖缀合的载体以产生针对该疾病的潜在有效疫苗。 还公开了使用P1肽缀合物作为免疫剂以引发抗流感嗜血杆菌b型抗体。