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1.
公开(公告)号:US20080014616A1
公开(公告)日:2008-01-17
申请号:US11827318
申请日:2007-07-11
IPC分类号: C12P19/34
CPC分类号: C12Q1/6853 , C12Q2525/161 , C12Q2525/119 , C12Q2525/113
摘要: Systems are disclosed that are useful for introducing one or more targeted positions or regions of diversity into a nucleic acid molecule. In certain embodiments, diversity in a targeted position or region is generated by providing one or more degenerate primer sets and a template nucleic acid molecule, wherein the primers are extended in opposite directions against the template nucleic acid molecule in a polymerase-mediated extension reaction. In certain embodiments, the generated nucleic acid molecule into which diversity has been introduced comprises single-stranded regions at its termini, which single-stranded regions are capable of annealing to each other.
摘要翻译: 公开了用于将一个或多个靶向位点或多样性区域引入核酸分子的系统。 在某些实施方案中,通过提供一个或多个简并引物组和模板核酸分子来产生靶向位点或区域的多样性,其中所述引物在聚合酶介导的延伸反应中相对于模板核酸分子在相反方向延伸。 在某些实施方案中,产生多样性的所产生的核酸分子在其末端包括单链区域,该单链区域能够相互退火。
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公开(公告)号:US20110030103A1
公开(公告)日:2011-02-03
申请号:US12596276
申请日:2008-04-16
摘要: Novel lipopeptides, and engineered polypeptides useful in synthesizing lipopeptides are provided. Also provided are methods of making lipopeptides using engineered polypeptides, and methods of using lipopeptides, e.g., as insecticidal and/or antimicrobial agents.
摘要翻译: 提供了新颖的脂肽和可用于合成脂肽的工程化多肽。 还提供了使用工程改造的多肽制备脂肽的方法,以及使用脂肽(例如杀虫剂和/或抗微生物剂)的方法。
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公开(公告)号:US20050239082A1
公开(公告)日:2005-10-27
申请号:US10763039
申请日:2004-01-22
申请人: Temple Smith , Prashanth Vishwanath , Sean Quinlan
发明人: Temple Smith , Prashanth Vishwanath , Sean Quinlan
CPC分类号: C12Q1/6876 , C12Q1/6837 , C12Q2600/166 , C12Q2545/114 , C12Q2545/101
摘要: The present invention provides sequences and reagents for preparing microarrays with internal controls. Specifically, the present invention defines and provides sequences that are not present in the hybridizing mRNA or cDNA, and therefore can be used both as hybridization controls and for inter-spot normalization.
摘要翻译: 本发明提供用于制备具有内部对照的微阵列的序列和试剂。 具体地,本发明定义并提供了不存在于杂交mRNA或cDNA中的序列,因此可以同时用作杂交控制和点间标准化。
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公开(公告)号:US07981685B2
公开(公告)日:2011-07-19
申请号:US12596272
申请日:2008-04-16
CPC分类号: C12N15/62 , C12N15/8243 , C12N15/8251 , C12N15/8257 , C12P13/02 , C12P13/04 , C12P13/14 , Y10T436/174614
摘要: Engineered polypeptides useful in synthesizing acyl amino acids are provided. Also provided are methods of making acyl amino acids using engineered polypeptides. In certain embodiments, an acyl amino acid produced using compositions and/or methods of the present invention comprises cocoyl glutamate.
摘要翻译: 提供了可用于合成酰基氨基酸的工程化多肽。 还提供了使用工程改造的多肽制备酰基氨基酸的方法。 在某些实施方案中,使用本发明的组合物和/或方法产生的酰基氨基酸包括椰油酰谷氨酸。
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公开(公告)号:US07396646B2
公开(公告)日:2008-07-08
申请号:US11224573
申请日:2005-09-12
申请人: Sean Quinlan , Temple Smith , Prashanth Vishwanath
发明人: Sean Quinlan , Temple Smith , Prashanth Vishwanath
CPC分类号: C12Q1/6837 , Y10S707/99936 , C12Q2545/101 , C12Q2545/114
摘要: The present invention provides sequences and reagents for preparing microarrays with internal controls. Specifically, the present invention defines and provides sequences that are not present in the hybridizing mRNA or cDNA, and therefore can be used both as hybridization controls and for inter-spot normalization.
摘要翻译: 本发明提供用于制备具有内部对照的微阵列的序列和试剂。 具体地,本发明定义并提供了不存在于杂交mRNA或cDNA中的序列,因此可以同时用作杂交控制和点间标准化。
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公开(公告)号:US20060134660A1
公开(公告)日:2006-06-22
申请号:US11224573
申请日:2005-09-12
申请人: Sean Quinlan , Temple Smith , Prashanth Vishwanath
发明人: Sean Quinlan , Temple Smith , Prashanth Vishwanath
CPC分类号: C12Q1/6837 , Y10S707/99936 , C12Q2545/101 , C12Q2545/114
摘要: The present invention provides sequences and reagents for preparing microarrays with internal controls. Specifically, the present invention defines and provides sequences that are not present in the hybridizing mRNA or cDNA, and therefore can be used both as hybridization controls and for inter-spot normalization.
摘要翻译: 本发明提供用于制备具有内部对照的微阵列的序列和试剂。 具体地,本发明定义并提供了不存在于杂交mRNA或cDNA中的序列,因此可以同时用作杂交控制和点间标准化。
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公开(公告)号:US20090143242A1
公开(公告)日:2009-06-04
申请号:US12168755
申请日:2008-07-07
申请人: Sean Quinlan , Temple Smith , Prashanth Vishwanath
发明人: Sean Quinlan , Temple Smith , Prashanth Vishwanath
CPC分类号: C12Q1/6837 , Y10S707/99936 , C12Q2545/101 , C12Q2545/114
摘要: The present invention provides sequences and reagents for preparing microarrays with internal controls. Specifically, the present invention defines and provides sequences that are not present in the hybridizing mRNA or cDNA, and therefore can be used both as hybridization controls and for inter-spot normalization.
摘要翻译: 本发明提供用于制备具有内部对照的微阵列的序列和试剂。 具体地,本发明定义并提供了不存在于杂交mRNA或cDNA中的序列,因此可以同时用作杂交控制和点间标准化。
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公开(公告)号:US20110030102A1
公开(公告)日:2011-02-03
申请号:US12596272
申请日:2008-04-16
CPC分类号: C12N15/62 , C12N15/8243 , C12N15/8251 , C12N15/8257 , C12P13/02 , C12P13/04 , C12P13/14 , Y10T436/174614
摘要: Engineered polypeptides useful in synthesizing acyl amino acids are provided. Also provided are methods of making acyl amino acids using engineered polypeptides. In certain embodiments, an acyl amino acid produced using compositions and/or methods of the present invention comprises cocoyl glutamate.
摘要翻译: 提供了可用于合成酰基氨基酸的工程化多肽。 还提供了使用工程改造的多肽制备酰基氨基酸的方法。 在某些实施方案中,使用本发明的组合物和/或方法产生的酰基氨基酸包含椰油酰谷氨酸。
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公开(公告)号:US09493800B2
公开(公告)日:2016-11-15
申请号:US13186001
申请日:2011-07-19
CPC分类号: C12N15/62 , C12N15/8243 , C12N15/8251 , C12N15/8257 , C12P13/02 , C12P13/04 , C12P13/14 , Y10T436/174614
摘要: Engineered polypeptides useful in synthesizing acyl amino acids are provided. Also provided are methods of making acyl amino acids using engineered polypeptides. In certain embodiments, an acyl amino acid produced using compositions and/or methods of the present invention comprises cocoyl glutamate.
摘要翻译: 提供了可用于合成酰基氨基酸的工程化多肽。 还提供了使用工程改造的多肽制备酰基氨基酸的方法。 在某些实施方案中,使用本发明的组合物和/或方法产生的酰基氨基酸包含椰油酰谷氨酸。
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公开(公告)号:US20130071885A1
公开(公告)日:2013-03-21
申请号:US13186001
申请日:2011-07-19
CPC分类号: C12N15/62 , C12N15/8243 , C12N15/8251 , C12N15/8257 , C12P13/02 , C12P13/04 , C12P13/14 , Y10T436/174614
摘要: Engineered polypeptides useful in synthesizing acyl amino acids are provided. Also provided are methods of making acyl amino acids using engineered polypeptides. In certain embodiments, an acyl amino acid produced using compositions and/or methods of the present invention comprises cocoyl glutamate.
摘要翻译: 提供了可用于合成酰基氨基酸的工程化多肽。 还提供了使用工程改造的多肽制备酰基氨基酸的方法。 在某些实施方案中,使用本发明的组合物和/或方法产生的酰基氨基酸包含椰油酰谷氨酸。
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