Methods of introducing targeted diversity into nucleic acid molecules
    1.
    发明申请
    Methods of introducing targeted diversity into nucleic acid molecules 审中-公开
    将目标多样性引入核酸分子的方法

    公开(公告)号:US20080014616A1

    公开(公告)日:2008-01-17

    申请号:US11827318

    申请日:2007-07-11

    IPC分类号: C12P19/34

    摘要: Systems are disclosed that are useful for introducing one or more targeted positions or regions of diversity into a nucleic acid molecule. In certain embodiments, diversity in a targeted position or region is generated by providing one or more degenerate primer sets and a template nucleic acid molecule, wherein the primers are extended in opposite directions against the template nucleic acid molecule in a polymerase-mediated extension reaction. In certain embodiments, the generated nucleic acid molecule into which diversity has been introduced comprises single-stranded regions at its termini, which single-stranded regions are capable of annealing to each other.

    摘要翻译: 公开了用于将一个或多个靶向位点或多样性区域引入核酸分子的系统。 在某些实施方案中,通过提供一个或多个简并引物组和模板核酸分子来产生靶向位点或区域的多样性,其中所述引物在聚合酶介导的延伸反应中相对于模板核酸分子在相反方向延伸。 在某些实施方案中,产生多样性的所产生的核酸分子在其末端包括单链区域,该单链区域能够相互退火。

    Alien sequences
    5.
    发明授权
    Alien sequences 失效
    外来序列

    公开(公告)号:US07396646B2

    公开(公告)日:2008-07-08

    申请号:US11224573

    申请日:2005-09-12

    IPC分类号: C07H21/04 C12Q1/68

    摘要: The present invention provides sequences and reagents for preparing microarrays with internal controls. Specifically, the present invention defines and provides sequences that are not present in the hybridizing mRNA or cDNA, and therefore can be used both as hybridization controls and for inter-spot normalization.

    摘要翻译: 本发明提供用于制备具有内部对照的微阵列的序列和试剂。 具体地,本发明定义并提供了不存在于杂交mRNA或cDNA中的序列,因此可以同时用作杂交控制和点间标准化。

    Alien sequences
    6.
    发明申请
    Alien sequences 失效
    外来序列

    公开(公告)号:US20060134660A1

    公开(公告)日:2006-06-22

    申请号:US11224573

    申请日:2005-09-12

    IPC分类号: C12Q1/68 C12M1/34

    摘要: The present invention provides sequences and reagents for preparing microarrays with internal controls. Specifically, the present invention defines and provides sequences that are not present in the hybridizing mRNA or cDNA, and therefore can be used both as hybridization controls and for inter-spot normalization.

    摘要翻译: 本发明提供用于制备具有内部对照的微阵列的序列和试剂。 具体地,本发明定义并提供了不存在于杂交mRNA或cDNA中的序列,因此可以同时用作杂交控制和点间标准化。

    ALIEN SEQUENCES
    7.
    发明申请
    ALIEN SEQUENCES 审中-公开
    ALIEN序列

    公开(公告)号:US20090143242A1

    公开(公告)日:2009-06-04

    申请号:US12168755

    申请日:2008-07-07

    IPC分类号: C40B30/04 C40B40/08

    摘要: The present invention provides sequences and reagents for preparing microarrays with internal controls. Specifically, the present invention defines and provides sequences that are not present in the hybridizing mRNA or cDNA, and therefore can be used both as hybridization controls and for inter-spot normalization.

    摘要翻译: 本发明提供用于制备具有内部对照的微阵列的序列和试剂。 具体地,本发明定义并提供了不存在于杂交mRNA或cDNA中的序列,因此可以同时用作杂交控制和点间标准化。