公开(公告)号：US20110065202A1

公开(公告)日：2011-03-17

申请号：US12992309

申请日：2009-06-11

申请人： Khin Than Myint ,  Yoshiya Oda ,  Naoki Asai

发明人： Khin Than Myint ,  Yoshiya Oda ,  Naoki Asai

IPC分类号： G01N33/53 ,  G01N27/26 ,  G01N30/02 ,  H01J49/26

CPC分类号： G01N33/9453 ,  G01N30/72 ,  G01N33/9486 ,  G01N2800/2821 ,  Y10T436/174614

摘要： A method for examining Alzheimer's disease comprising (1) the measurement step of measuring an amount of lidocaine or a metabolite thereof in a cerebrospinal fluid derived from a lidocaine-administered test subject, and (2) the determination step of determining whether the test subject has Alzheimer's disease or not on the basis of the measured amount.

摘要翻译： 一种检查阿尔茨海默氏病的方法，其特征在于，包括：（1）测定利多卡因或其代谢物在来自利多卡因的测试对象的脑脊液中的量的测定步骤，以及（2） 阿尔茨海默氏病或​​不是基于测量量。

公开(公告)号：US07759130B2

公开(公告)日：2010-07-20

申请号：US10579780

申请日：2004-11-22

申请人： Yoshiya Oda ,  Yasushi Ishihama ,  Tsuyoshi Tabata

发明人： Yoshiya Oda ,  Yasushi Ishihama ,  Tsuyoshi Tabata

IPC分类号： G01N24/00

CPC分类号： G01N33/6848 ,  Y10T436/10 ,  Y10T436/104165 ,  Y10T436/104998 ,  Y10T436/105831 ,  Y10T436/24

摘要： An object of the present invention is to quantitate with good accuracy, furthermore, quantitate absolutely, one or a plurality of biological molecules in a sample such as a tissue, a biological fluid, a cell, a cell organ or protein complex.By adding a metabolically isotope labeled biological molecule as an internal standard substance and measuring with a mass spectrometer, quantitating with good accuracy one or a plurality of target molecules in a sample has become possible. In addition, by performing waveform separation processing during mass analysis, a highly accurate quantitative analysis method of mass analysis is provided.

摘要翻译： 本发明的目的是准确定量，另外，在样品如组织，生物流体，细胞，细胞器官或蛋白质复合物中绝对地定量分析一种或多种生物分子。 通过添加代谢同位素标记的生物分子作为内标物质并用质谱仪进行测量，以高精度定量，样品中的一个或多个靶分子已成为可能。 此外，通过在质量分析中进行波形分离处理，提供了高精度的质量分析的定量分析方法。

公开(公告)号：US5354461A

公开(公告)日：1994-10-11

申请号：US39511

申请日：1993-03-29

申请人： Naoki Asakawa ,  Yoshiya Oda ,  Yutaka Yoshida ,  Tadashi Sato

发明人： Naoki Asakawa ,  Yoshiya Oda ,  Yutaka Yoshida ,  Tadashi Sato

IPC分类号： B01D15/08 ,  B01J20/32

CPC分类号： B01D15/3823 ,  B01J20/287 ,  B01J20/289 ,  B01J20/29 ,  B01J20/3204 ,  B01J20/3219 ,  B01J20/3251 ,  B01J20/3255 ,  B01J20/3274 ,  B01J2220/54 ,  B01J2220/58

摘要： A packing for chromatography whereby a low molecular weight substance contained in biological samples can be analyzed directly at a high accuracy without any pretreatments. This packing is combining a protein such as avidin and ovomucoid to the support via a straight or branched spacer optionally having a functional group such as a cyano group. Enantiomers interact differentially with proteins, and thereby optically separated. Further, a purification column packed with said packing is disclosed.According to the present invention, a low molecular weight substance can be directly and accurately analyzed without requiring any pretreatment for removing biological macromolecules. When it is employed in the purification column, the time required for the pretreatment can be shortened.

摘要翻译： 用于色谱的填料，其中可以以高精度直接分析生物样品中所含的低分子量物质，而无需任何预处理。 这种包装是将蛋白质如抗生物素蛋白和卵类粘蛋白通过任选具有官能团例如氰基的直链或支链间隔与载体组合。 对映异构体与蛋白质差异化，从而光学分离。 此外，公开了一种填充有所述填料的净化塔。 根据本发明，可以直接且精确地分析低分子量物质，而不需要任何预处理来除去生物大分子。 当在纯化塔中使用时，可以缩短预处理所需的时间。

公开(公告)号：US08131479B2

公开(公告)日：2012-03-06

申请号：US11630927

申请日：2005-07-04

申请人： Yoshiya Oda ,  Tsuyoshi Tabata

发明人： Yoshiya Oda ,  Tsuyoshi Tabata

IPC分类号： G06F19/00 ,  G01R23/02

CPC分类号： G01N33/6842 ,  B01J20/286 ,  B01J2220/54 ,  C07K1/22 ,  G01N30/8675 ,  G01N33/6848

摘要： A method for detecting plural types of phosphorylated proteins in a sample, wherein a database consisting of data regarding plural types of proteins in the sample is used; and a method for purifying phosphorylated proteins using an immobilized metal carrier or a titania carrier, wherein a solution containing acetonitrile in a range of 40% (v/v) or greater but 60% (v/v) or less is used.

摘要翻译： 一种用于检测样品中多种磷酸化蛋白质的方法，其中使用由样本中关于多种蛋白质的数据组成的数据库; 以及使用固定金属载体或二氧化钛载体来纯化磷酸化蛋白质的方法，其中使用含有40％（v / v）以上且60％（v / v）以下范围内的乙腈的溶液。

公开(公告)号：US20090148960A1

公开(公告)日：2009-06-11

申请号：US11989565

申请日：2006-07-31

申请人： Yoshiya Oda ,  Hiroyuki Katayama

发明人： Yoshiya Oda ,  Hiroyuki Katayama

IPC分类号： G01N33/543 ,  B01J19/00

CPC分类号： G01N33/6848 ,  G01N30/72 ,  G01N30/84 ,  G01N33/54306 ,  G01N33/58 ,  G01N2030/027 ,  G01N2030/8411 ,  G01N2458/15 ,  G01N2560/00 ,  H01J49/0409 ,  Y10T436/24

摘要： The present invention provides a method for analyzing a binding ability of protein to a compound, comprising the steps of (a) fractionating a first group of isotope-labeled proteins into plural fractions using a carrier having the compound immobilized thereon; (b) fractionating a second group of proteins into one or plural fractions using a carrier having the compound immobilized thereon; (c) adding a certain amount of the one fraction obtained in step (b), or a certain amount of a mixture of all the fractions or a mixture of plural contiguous fractions among the fractions obtained in step (b), to each of the fractions obtained in step (a); (d) analyzing the fractions obtained in step (c) with mass spectrometry; and (e) based on the mass spectrometry information, obtaining, regarding each fraction, an intensity ratio between a peak derived from a protein in the fraction obtained in step (a) and a peak derived from a protein in the fraction obtained in step (b), and comparing degrees of the binding ability of the plural kinds of proteins to the compound.

摘要翻译： 本发明提供了一种用于分析蛋白质与化合物的结合能力的方法，包括以下步骤：（a）使用其上固定有化合物的载体将第一组同位素标记的蛋白质分馏成多个级分; （b）使用其上固定化合物的载体将第二组蛋白质分馏成一个或多个级分; （c）将步骤（b）中获得的一定量的一种级分或一定量的所有级分的混合物或步骤（b）中获得的级分中的多个相邻级分的混合物加入到 步骤（a）中获得的级分; （d）用质谱分析步骤（c）中得到的级分; 和（e）基于质谱信息，从每个级分获得在步骤（a）中获得的级分中衍生自蛋白质的峰与来自步骤（a）中获得的级分中的蛋白质的峰值之间的强度比， b），并且比较多种蛋白质与化合物的结合能力的程度。

公开(公告)号：US08889423B2

公开(公告)日：2014-11-18

申请号：US11989565

申请日：2006-07-31

申请人： Yoshiya Oda ,  Hiroyuki Katayama

发明人： Yoshiya Oda ,  Hiroyuki Katayama

IPC分类号： G01N30/02 ,  G01N24/00 ,  C07K1/22 ,  G01N30/84 ,  H01J49/04 ,  G01N33/68 ,  G01N30/72

CPC分类号： G01N33/6848 ,  G01N30/72 ,  G01N30/84 ,  G01N33/54306 ,  G01N33/58 ,  G01N2030/027 ,  G01N2030/8411 ,  G01N2458/15 ,  G01N2560/00 ,  H01J49/0409 ,  Y10T436/24

摘要： A method for analyzing a binding ability of protein to a compound, comprising the steps of (a) fractionating a first group of isotope-labeled proteins into plural fractions using a carrier having the compound immobilized thereon; (b) fractionating a second group of proteins into one or plural fractions using a carrier having the compound immobilized thereon; (c) adding an amount of at least one fraction obtained in step (b) to each of the fractions obtained in step (a); (d) analyzing the fractions obtained in step (c) with mass spectrometry; and (e) based on the mass spectrometry information, obtaining, regarding each fraction, an intensity ratio between a peak derived from a protein in the fraction obtained in step (a) and a peak derived from a protein in the fraction obtained in step (b), and comparing degrees of the binding ability of the plural kinds of proteins to the compound.

摘要翻译： 一种用于分析蛋白质与化合物的结合能力的方法，包括以下步骤：（a）使用其上固定有化合物的载体将第一组同位素标记的蛋白质分馏成多个级分; （b）使用其上固定化合物的载体将第二组蛋白质分馏成一个或多个级分; （c）将步骤（b）中获得的至少一个级分的量加入到步骤（a）中获得的每种级分中; （d）用质谱分析步骤（c）中得到的级分; 和（e）基于质谱信息，从每个级分获得在步骤（a）中获得的级分中衍生自蛋白质的峰与来自步骤（a）中获得的级分中的蛋白质的峰值之间的强度比， b），并且比较多种蛋白质与化合物的结合能力的程度。

公开(公告)号：US08030083B2

公开(公告)日：2011-10-04

申请号：US11571337

申请日：2005-07-04

申请人： Yoshiya Oda

发明人： Yoshiya Oda

IPC分类号： G01N33/00

CPC分类号： G01N33/6803 ,  B01J20/286 ,  B01J20/3244 ,  B01J2220/54 ,  G01N33/6848 ,  G01N2500/02 ,  Y10T436/24

摘要： A method for analyzing a structural affinity relationship between plural kinds of proteins and a compound, comprising the steps of (a) using a compound-immobilized carrier to purify plural kinds of proteins bound to the compound on the carrier, from a group of isotope-labeled proteins; (b) using a compound-immobilized carrier to purify plural kinds of proteins bound to the compound on the carrier, from a group of proteins brought into contact with a compound beforehand; (c) mixing the proteins obtained in step (a) and step (b); (d) analyzing the mixture obtained in step (c) with mass spectrometry; (e) identifying each of plural kinds of proteins based on information obtained by the mass spectrometry; and (f) obtaining an intensity ratio between a labeled peak and a non-labeled peak of each protein, thereby quantitating an affinity ratio of the compound to each protein.

摘要翻译： 一种用于分析多种蛋白质和化合物之间的结构亲和关系的方法，包括以下步骤：（a）使用固定化合物的载体从载体上与所述化合物结合的多种蛋白质从一组​​同位素 - 标记蛋白; （b）使用化合物固定化载体从预先与化合物接触的一组蛋白质中纯化与载体上的化合物结合的多种蛋白质; （c）混合步骤（a）和步骤（b）中获得的蛋白质; （d）用质谱分析步骤（c）中得到的混合物; （e）基于通过质谱法获得的信息来鉴定多种蛋白质中的每一种; 和（f）获得每种蛋白质的标记峰和非标记峰之间的强度比，从而定量化合物与每种蛋白质的亲和比。

公开(公告)号：US20070134806A1

公开(公告)日：2007-06-14

申请号：US10579780

申请日：2004-11-22

申请人： Yoshiya Oda ,  Yasushi Ishihama ,  Tsuyoshi Tabata

发明人： Yoshiya Oda ,  Yasushi Ishihama ,  Tsuyoshi Tabata

IPC分类号： G01N24/00

CPC分类号： G01N33/6848 ,  Y10T436/10 ,  Y10T436/104165 ,  Y10T436/104998 ,  Y10T436/105831 ,  Y10T436/24

摘要： Problem: An object of the present invention is to quantitate with good accuracy, furthermore, quantitate absolutely, one or a plurality of biological molecules in a sample such as a tissue, a biological fluid, a cell, a cell organ or protein complex. Solution: By adding a metabolically isotope labeled biological molecule as an internal standard substance and measuring with a mass spectrometer, quantitating with good accuracy one or a plurality of target molecules in a sample has become possible. In addition, by performing waveform separation processing during mass analysis, a highly accurate quantitative analysis method of mass analysis is provided.

摘要翻译： 问题：本发明的目的是准确定量，此外，在样品如组织，生物流体，细胞，细胞器官或蛋白质复合物中绝对地定量分析一种或多种生物分子。 解决方案：通过添加代谢同位素标记的生物分子作为内标物质并用质谱仪进行测量，以高精度定量，样品中的一个或多个目标分子成为可能。 此外，通过在质量分析中进行波形分离处理，提供了高精度的质量分析的定量分析方法。

公开(公告)号：US5480542A

公开(公告)日：1996-01-02

申请号：US294113

申请日：1994-08-22

申请人： Naoki Asakawa ,  Yoshiya Oda ,  Yutaka Yoshida ,  Tadashi Sato

发明人： Naoki Asakawa ,  Yoshiya Oda ,  Yutaka Yoshida ,  Tadashi Sato

IPC分类号： B01J20/283 ,  B01D15/08 ,  B01J20/281 ,  B01J20/285 ,  B01J20/286 ,  B01J20/291 ,  B01J20/32 ,  C07K17/00 ,  C07K17/06 ,  C07K17/14 ,  G01N30/88

CPC分类号： G01N30/482 ,  B01D15/3809 ,  B01D15/3823 ,  B01J20/289 ,  B01J20/29 ,  B01J20/3204 ,  B01J20/3208 ,  B01J20/3212 ,  B01J20/3219 ,  B01J20/3274 ,  B01D15/3833 ,  B01J2220/54

摘要： A packing for chromatography whereby a low molecular weight substance contained in biological samples can be analyzed directly at a high accuracy without any pretreatments. This packing is combining a protein such as avidin and ovomucoid to the support via a straight or branched spacer optionally having a functional group such as a cyano group. Enantiomers interact differentially with proteins, and thereby optically separated. Further, a purification column packed with said packing is disclosed. According to the present invention, a low molecular weight substance can be directly and accurately analyzed without requiring any pretreatment for removing biological macromolecules. When it is employed in the purification column, the time required for the pretreatment can be shortened.

摘要翻译： 用于色谱的填料，其中可以以高精度直接分析生物样品中所含的低分子量物质，而无需任何预处理。 这种包装是将蛋白质如抗生物素蛋白和卵类粘蛋白通过任选具有官能团例如氰基的直链或支链间隔与载体组合。 对映异构体与蛋白质差异化，从而光学分离。 此外，公开了一种填充有所述填料的净化塔。 根据本发明，可以直接且精确地分析低分子量物质，而不需要任何预处理来除去生物大分子。 当在纯化塔中使用时，可以缩短预处理所需的时间。

公开(公告)号：US5117109A

公开(公告)日：1992-05-26

申请号：US578267

申请日：1990-09-06

申请人： Naoki Asakawa ,  Hiroshi Ohe ,  Yutaka Yoshida ,  Tadashi Sato ,  Yukuo Nezu ,  Yoshiya Oda

发明人： Naoki Asakawa ,  Hiroshi Ohe ,  Yutaka Yoshida ,  Tadashi Sato ,  Yukuo Nezu ,  Yoshiya Oda

IPC分类号： G01N27/62 ,  G01N30/26 ,  G01N30/72 ,  G01N30/80 ,  G01N30/84

CPC分类号： G01N30/728 ,  G01N30/80 ,  G01N2030/8411

摘要： The components present in the sample with a mobile phase A in 1st section is separated. In 2nd section there is provided with an inserting line of a dilute solution for diluting the mobile phase A and a trapping column which forces the dilute solution to pass but catches the components by connecting said line to said column. In 3rd section, the components caught by the trapping column are introduced into mass spectrometer by using a mobile phase B. It is preferable to use respectively different mobile phases for the mobile phase A and the mobile phase B. It is good to install separating columns in 1st and 3rd sections. It is also desired to add the retaining apparatus of the components separated in 1st section.

摘要翻译： 第一部分中流动相A的样品中存在的组分被分离。 在第二部分中，设置有用于稀释流动相A的稀释溶液的插入线和迫使稀释溶液通过的捕集柱，但是通过将所述管线连接到所述柱而捕获部件。 在第3部分中，捕集塔捕获的成分通过使用流动相B引入质谱仪。优选在流动相A和流动相B中分别使用不同的流动相。安装分离塔是很好的 在第1和第3部分。 还希望添加在第一部分中分离的部件的保持装置。