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公开(公告)号：US20120135235A1
公开(公告)日：2012-05-31
申请号：US13125306
申请日：2009-10-21
CPC分类号： C08J3/075 , G03F7/0037 , G03F7/2012 , G03F7/2035 , Y10T428/2982
摘要： Techniques are provided to independently control 3D shape and chemistry of rapidly produced colloids. A pre-polymer mixture including a monomer is made to flow into a channel with insular relief in a wall at a known location of the channel. A stimulus that polymerizes the pre-polymer mixture is directed onto the known location to form a structure locked in place at the known location by the insular relief. A pressure is applied to the channel that is sufficient to deflect the wall having the insular relief sufficiently to release a hydrogel particle comprising the structure.
摘要翻译： 提供了独立控制快速生成的胶体的3D形状和化学性质的技术。 使包含单体的预聚合物混合物在通道的已知位置的壁中流入具有岛状浮雕的通道。 将预聚合物混合物聚合的刺激物被引导到已知位置，以形成通过岛状浮雕锁定在已知位置的结构。 对通道施加足以使具有岛状浮雕的壁充分偏转以释放包含该结构的水凝胶颗粒的压力。
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公开(公告)号：US09512278B2
公开(公告)日：2016-12-06
申请号：US13125306
申请日：2009-10-21
CPC分类号： C08J3/075 , G03F7/0037 , G03F7/2012 , G03F7/2035 , Y10T428/2982
摘要： Techniques are provided to independently control 3D shape and chemistry of rapidly produced colloids. A pre-polymer mixture including a monomer is made to flow into a channel with insular relief in a wall at a known location of the channel. A stimulus that polymerizes the pre-polymer mixture is directed onto the known location to form a structure locked in place at the known location by the insular relief. A pressure is applied to the channel that is sufficient to deflect the wall having the insular relief sufficiently to release a hydrogel particle comprising the structure.
摘要翻译： 提供了独立控制快速生成的胶体的3D形状和化学性质的技术。 使包含单体的预聚合物混合物在通道的已知位置的壁中流入具有岛状浮雕的通道。 将预聚合物混合物聚合的刺激物被引导到已知位置，以形成通过岛状浮雕锁定在已知位置的结构。 对通道施加足以使具有岛状浮雕的壁充分偏转以释放包含该结构的水凝胶颗粒的压力。
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3.发明申请公开(公告)号：US20110136104A1
公开(公告)日：2011-06-09
申请号：US12631322
申请日：2009-12-04
CPC分类号： C12Q1/6851 , C12Q1/6858 , C12Q2537/143 , C12Q2545/101
摘要： Certain embodiments of the present invention are directed to one pot multiplexed quantitative PCR methods for end point analysis of a plurality of nucleic acid targets in a complex sample without user intervention, and to various encoded particles on which are immobilized one or more probes that hybridize with the plurality of targets. Certain other embodiments are directed to a new “multiple-color genetic variation detection method” that can detect SNPs and kit using one chamber multiplexed endpoint PCR and differentially labeled allele-specific primers (one recognizing only the wild type allele and one only the mutant allele).
摘要翻译： 本发明的某些实施方案涉及一种用于无需用户干预的复合体样品中的多个核酸靶的终点分析的多重定量PCR方法，以及固定有一种或多种与 多个目标。 某些其他实施方案涉及可以使用一室复合端点PCR和差异标记的等位基因特异性引物（一个仅识别野生型等位基因并且仅一个仅突变等位基因）检测SNP和试剂盒的新的“多色遗传变异检测方法” ）。
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公开(公告)号：US20150234270A1
公开(公告)日：2015-08-20
申请号：US14702648
申请日：2015-05-01
CPC分类号： G03F7/0037 , B81C99/0095 , B81C2201/0159 , G02B1/04 , G03F7/2012 , G03F7/2035 , G03F7/2053 , H01B1/20 , H01F1/42 , Y10T428/1352 , Y10T428/24 , Y10T428/24273 , Y10T428/24298
摘要： In a method for synthesizing polymeric microstructures, a monomer stream is flowed, at a selected flow rate, through a fluidic channel. At least one shaped pulse of illumination is projected to the monomer stream, defining in the monomer stream a shape of at least one microstructure corresponding to the illumination pulse shape while polymerizing that microstructure shape in the monomer stream by the illumination pulse. An article of manufacture includes a non-spheroidal polymeric microstructure that has a plurality of distinct material regions.
摘要翻译： 在合成聚合物微结构的方法中，单体流以选定的流速通过流体通道流动。 将至少一个成形的照明脉冲投射到单体流，在单体流中限定与照明脉冲形状对应的至少一个微结构的形状，同时通过照射脉冲聚合单体流中的微结构形状。 制品包括具有多个不同材料区域的非球形聚合物微结构。
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5.发明申请公开(公告)号：US20120107820A1
公开(公告)日：2012-05-03
申请号：US13350667
申请日：2012-01-13
CPC分类号： C12Q1/6851 , C12Q1/6858 , C12Q2537/143 , C12Q2545/101
摘要： Certain embodiments of the present invention are directed to one pot multiplexed quantitative PCR methods for end point analysis of a plurality of nucleic acid targets in a complex sample without user intervention, and to various encoded particles on which are immobilized one or more probes that hybridize with the plurality of targets. Certain other embodiments are directed to a new “multiple-color genetic variation detection method” that can detect SNPs and kit using one chamber multiplexed endpoint PCR and differentially labeled allele-specific primers (one recognizing only the wild type allele and one only the mutant allele).
摘要翻译： 本发明的某些实施方案涉及一种用于无需用户干预的复合体样品中的多个核酸靶的终点分析的多重定量PCR方法，以及固定有一种或多种与 多个目标。 某些其他实施方案涉及可以使用一室复合端点PCR和差异标记的等位基因特异性引物（一个仅识别野生型等位基因并且仅一个仅突变等位基因）检测SNP和试剂盒的新的“多色遗传变异检测方法” ）。
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公开(公告)号：US08535644B2
公开(公告)日：2013-09-17
申请号：US12578303
申请日：2009-10-13
CPC分类号： C08J3/075 , A61K47/68 , A61K47/6921 , C08J2371/02 , C08L2205/14 , C09D7/20 , C09D7/70 , Y10T428/2982
摘要： Techniques are provided to produce and use non-spherical colloidal particles with independently tuned size, shape, flexibility, and chemical properties. A pre-polymer mixture for forming hydrogel particles includes a percentage of PEGDA selected to impart a target stiffness to the particles and includes, a percentage of acrylic acid selected to impart an independent target chemical function to the particles. The mixture also includes a percentage of photo-initiator to polymerize PEGDA upon exposure to a light source to impart an independently selected target size or shape or both to the particles.
摘要翻译： 提供技术来生产和使用具有独立调整的尺寸，形状，柔性和化学性质的非球形胶体颗粒。 用于形成水凝胶颗粒的预聚物混合物包括选择用于赋予颗粒目标硬度的百分比的PEGDA，并且包括一定百分比的丙烯酸，其被选择以赋予颗粒独立的目标化学功能。 该混合物还包括一定百分比的光引发剂，以在暴露于光源时聚合PEGDA以赋予颗粒独立选择的靶尺寸或形状或两者。
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公开(公告)号：US20120003755A1
公开(公告)日：2012-01-05
申请号：US13230790
申请日：2011-09-12
IPC分类号： G01N21/75
CPC分类号： B01L3/502761 , B01L2200/0647 , B01L2300/0816 , B01L2300/123 , B01L2400/084 , Y10T428/2982 , Y10T436/117497 , Y10T436/2575
摘要： Techniques are provided for high precision scanning of hydrogel microparticles. The high precision is achieved by one or more modifications to the microparticle composition, or microfluidics apparatus that align the microparticles in a detection channel, or method of preparing a sample for introduction into the apparatus, or some combination. An apparatus comprises a body structure having formed therein a central channel and multiple focusing channels in fluid communication with the central channel through multiple junctions. A width of the central channel is smaller in a portion downstream of each junction. A particle comprises a hydrogel matrix and a probe molecule. The particle has an aspect ratio greater than about three. A method includes loading into a sample fluid inlet a mixture, wherein a number of particles lies within a range from about 5 to about 10 particles/μl.
摘要翻译： 提供技术用于水凝胶微粒的高精度扫描。 通过对微粒组合物的一个或多个修改或者将微粒对准检测通道的微流体装置或者制备用于引入到装置中的样品的方法或一些组合来实现高精度。 一种装置包括其中形成有中心通道的主体结构和通过多个连接部与中心通道流体连通的多个聚焦通道。 中央通道的宽度在每个连接点的下游部分较小。 颗粒包含水凝胶基质和探针分子。 颗粒的纵横比大于约3。 一种方法包括将样品流体入口装载到混合物中，其中许多颗粒位于约5至约10个颗粒/μl的范围内。
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公开(公告)号：US20100330693A1
公开(公告)日：2010-12-30
申请号：US12552268
申请日：2009-09-01
CPC分类号： B01L3/502761 , B01L2200/0647 , B01L2300/0816 , B01L2300/123 , B01L2400/084 , Y10T428/2982 , Y10T436/117497 , Y10T436/2575
摘要： Techniques are provided for high precision scanning of hydrogel microparticles. The high precision is achieved by one or more modifications to the microparticle composition, or microfluidics apparatus that align the microparticles in a detection channel, or method of preparing a sample for introduction into the apparatus, or some combination. An apparatus comprises a body structure having formed therein a central channel and multiple focusing channels in fluid communication with the central channel through multiple junctions. A width of the central channel is smaller in a portion downstream of each junction. A particle comprises a hydrogel matrix and a probe molecule. The particle has an aspect ratio greater than about three. A method includes loading into a sample fluid inlet a mixture, wherein a number of particles lies within a range from about 15 to about 20 particles/μl.
摘要翻译： 提供技术用于水凝胶微粒的高精度扫描。 通过对微粒组合物的一个或多个修改或者将微粒对准检测通道的微流体装置或者制备用于引入到装置中的样品的方法或一些组合来实现高精度。 一种装置包括其中形成有中心通道的主体结构和通过多个连接部与中心通道流体连通的多个聚焦通道。 中央通道的宽度在每个连接点的下游部分较小。 颗粒包含水凝胶基质和探针分子。 颗粒的纵横比大于约3。 一种方法包括将样品流体入口装载到混合物中，其中许多颗粒位于约15至约20个颗粒/μl的范围内。
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公开(公告)号：US20100092393A1
公开(公告)日：2010-04-15
申请号：US12578303
申请日：2009-10-13
CPC分类号： C08J3/075 , A61K47/68 , A61K47/6921 , C08J2371/02 , C08L2205/14 , C09D7/20 , C09D7/70 , Y10T428/2982
摘要： Techniques are provided to produce and use non-spherical colloidal particles with independently tuned size, shape, flexibility, and chemical properties. A pre-polymer mixture for forming hydrogel particles includes a percentage of PEGDA selected to impart a target stiffness to the particles and includes, a percentage of acrylic acid selected to impart an independent target chemical function to the particles. The mixture also includes a percentage of photo-initiator to polymerize PEGDA upon exposure to a light source to impart an independently selected target size or shape or both to the particles.
摘要翻译： 提供技术来生产和使用具有独立调整的尺寸，形状，柔性和化学性质的非球形胶体颗粒。 用于形成水凝胶颗粒的预聚物混合物包括选择用于赋予颗粒目标硬度的百分比的PEGDA，并且包括一定百分比的丙烯酸，其被选择以赋予颗粒独立的目标化学功能。 该混合物还包括一定百分比的光引发剂，以在暴露于光源时聚合PEGDA以赋予颗粒独立选择的靶尺寸或形状或两者。
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公开(公告)号：US09910352B2
公开(公告)日：2018-03-06
申请号：US14702648
申请日：2015-05-01
CPC分类号： G03F7/0037 , B81C99/0095 , B81C2201/0159 , G02B1/04 , G03F7/2012 , G03F7/2035 , G03F7/2053 , H01B1/20 , H01F1/42 , Y10T428/1352 , Y10T428/24 , Y10T428/24273 , Y10T428/24298
摘要： In a method for synthesizing polymeric microstructures, a monomer stream is flowed, at a selected flow rate, through a fluidic channel. At least one shaped pulse of illumination is projected to the monomer stream, defining in the monomer stream a shape of at least one microstructure corresponding to the illumination pulse shape while polymerizing that microstructure shape in the monomer stream by the illumination pulse. An article of manufacture includes a non-spheroidal polymeric microstructure that has a plurality of distinct material regions.
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