公开(公告)号：US08048312B2

公开(公告)日：2011-11-01

申请号：US12685724

申请日：2010-01-12

申请人： Kisaburo Deguchi ,  Shinya Ito ,  Masahito Ito

发明人： Kisaburo Deguchi ,  Shinya Ito ,  Masahito Ito

IPC分类号： B01D15/08

CPC分类号： G01N35/1097 ,  G01N1/38 ,  G01N30/20 ,  G01N30/34 ,  G01N2030/027 ,  Y10T436/2575

摘要： A separation analyzer for transferring solutions, while changing mixing ratios of the solutions, wherein a flow passage from a first pump, a flow passage from a second pump, and a passage for sample introduction section, the flow passages being connected, which further comprises a flow switching means for temporarily storing the solutions, whereby the flow switching means alternatively makes a first state for pushing out the solutions in a second sampling loop to the sample introduction section by the second pump, while transferring the solutions to the first sampling loop; and a second state for pushing out the solutions in the first sampling loop by the second pump, while transferring the solutions from the first pump to the second sampling loop.

摘要翻译： 一种用于输送溶液的分离器，同时改变溶液的混合比，其中来自第一泵的流动通道，来自第二泵的流动通道和用于样品引入部分的通道，流动通道连接，还包括 流动切换装置，用于临时存储溶液，由此流动切换装置交替地将第二状态用于将第二取样回路中的溶液推出到第二泵的样品引入部分，同时将溶液转移到第一取样回路; 以及第二状态，用于在将所述解决方案从所述第一泵传送到所述第二采样回路的同时，通过所述第二泵推出所述第一采样回路中的所述解。

公开(公告)号：US07699990B2

公开(公告)日：2010-04-20

申请号：US11581147

申请日：2006-10-16

申请人： Kisaburo Deguchi ,  Shinya Ito ,  Masahito Ito

发明人： Kisaburo Deguchi ,  Shinya Ito ,  Masahito Ito

IPC分类号： B01D15/08

CPC分类号： G01N35/1097 ,  G01N1/38 ,  G01N30/20 ,  G01N30/34 ,  G01N2030/027 ,  Y10T436/2575

摘要： A separation analyzer for transferring solutions, while changing mixing ratios of the solutions, wherein a flow passage from a first pump, a flow passage from a second pump, and a passage for sample introduction section, the flow passages being connected, which further comprises a flow switching means for temporarily storing the solutions, whereby the flow switching means alternatively makes a first state for pushing out the solutions in a second sampling loop to the sample introduction section by the second pump, while transferring the solutions to the first sampling loop; and a second state for pushing out the solutions in the first sampling loop by the second pump, while transferring the solutions from the first pump to the second sampling loop.

摘要翻译： 一种用于输送溶液的分离器，同时改变溶液的混合比，其中来自第一泵的流动通道，来自第二泵的流动通道和用于样品引入部分的通道，流动通道连接，还包括 流动切换装置，用于临时存储溶液，由此流动切换装置交替地将第二状态用于将第二取样回路中的溶液推出到第二泵的样品引入部分，同时将溶液转移到第一取样回路; 以及第二状态，用于在将所述解决方案从所述第一泵传送到所述第二采样回路的同时，通过所述第二泵推出所述第一采样回路中的所述解。

公开(公告)号：US20100107782A1

公开(公告)日：2010-05-06

申请号：US12685724

申请日：2010-01-12

申请人： Kisaburo Deguchi ,  Shinya Ito ,  Masahito Ito

发明人： Kisaburo Deguchi ,  Shinya Ito ,  Masahito Ito

IPC分类号： G01N1/14 ,  G01N1/44

CPC分类号： G01N35/1097 ,  G01N1/38 ,  G01N30/20 ,  G01N30/34 ,  G01N2030/027 ,  Y10T436/2575

摘要： A separation analyzer for transferring solutions, while changing mixing ratios of the solutions, wherein a flow passage from a first pump, a flow passage from a second pump, and a passage for sample introduction section, the flow passages being connected, which further comprises a flow switching means for temporarily storing the solutions, whereby the flow switching means alternatively makes a first state for pushing out the solutions in a second sampling loop to the sample introduction section by the second pump, while transferring the solutions to the first sampling loop; and a second state for pushing out the solutions in the first sampling loop by the second pump, while transferring the solutions from the first pump to the second sampling loop.

摘要翻译： 一种用于输送溶液的分离器，同时改变溶液的混合比，其中来自第一泵的流动通道，来自第二泵的流动通道和用于样品引入部分的通道，流动通道连接，还包括 流动切换装置，用于临时存储溶液，由此流动切换装置交替地将第二状态用于将第二取样回路中的溶液推出到第二泵的样品引入部分，同时将溶液转移到第一取样回路; 以及第二状态，用于在将所述解决方案从所述第一泵传送到所述第二采样回路的同时，通过所述第二泵推出所述第一采样回路中的所述解。

公开(公告)号：US20070031285A1

公开(公告)日：2007-02-08

申请号：US11581147

申请日：2006-10-16

申请人： Kisaburo Deguchi ,  Shinya Ito ,  Masahito Ito

发明人： Kisaburo Deguchi ,  Shinya Ito ,  Masahito Ito

IPC分类号： G01N33/00

CPC分类号： G01N35/1097 ,  G01N1/38 ,  G01N30/20 ,  G01N30/34 ,  G01N2030/027 ,  Y10T436/2575

摘要： A separation analyzer for transferring solutions, while changing mixing ratios of the solutions, wherein a flow passage from a first pump, a flow passage from a second pump, and a passage for sample introduction section, the flow passages being connected, which further comprises a flow switching means for temporarily storing the solutions, whereby the flow switching means alternatively makes a first state for pushing out the solutions in a second sampling loop to the sample introduction section by the second pump, while transferring the solutions to the first sampling loop; and a second state for pushing out the solutions in the first sampling loop by the second pump, while transferring the solutions from the first pump to the second sampling loop.

摘要翻译： 一种用于输送溶液的分离器，同时改变溶液的混合比，其中来自第一泵的流动通道，来自第二泵的流动通道和用于样品引入部分的通道，流动通道连接，还包括 流动切换装置，用于临时存储溶液，由此流动切换装置交替地将第二状态用于将第二取样回路中的溶液推出到第二泵的样品引入部分，同时将溶液转移到第一取样回路; 以及第二状态，用于在将所述解决方案从所述第一泵传送到所述第二采样回路的同时，通过所述第二泵推出所述第一采样回路中的所述解。

公开(公告)号：US07135111B2

公开(公告)日：2006-11-14

申请号：US10776263

申请日：2004-02-12

申请人： Kisaburo Deguchi ,  Shinya Ito ,  Masahito Ito

发明人： Kisaburo Deguchi ,  Shinya Ito ,  Masahito Ito

IPC分类号： B01D15/08

CPC分类号： G01N35/1097 ,  G01N1/38 ,  G01N30/20 ,  G01N30/34 ,  G01N2030/027 ,  Y10T436/2575

摘要： A separation analyzer for transferring solutions, while changing mixing ratios of the solutions, wherein a flow passage from a first pump, a flow passage from a second pump, and a passage for sample introduction section, the flow passages being connected, which further comprises a flow switching means for temporarily storing the solutions, whereby the flow switching means alternatively makes a first state for pushing out the solutions in a second sampling loop to the sample introduction section by the second pump, while transferring the solutions to the first sampling loop; and a second state for pushing out the solutions in the first sampling loop by the second pump, while transferring the solutions from the first pump to the second sampling loop.

摘要翻译： 一种用于输送溶液的分离器，同时改变溶液的混合比，其中来自第一泵的流动通道，来自第二泵的流动通道和用于样品引入部分的通道，流动通道连接，还包括 流动切换装置，用于临时存储溶液，由此流动切换装置交替地将第二状态用于将第二取样回路中的溶液推出到第二泵的样品引入部分，同时将溶液转移到第一取样回路; 以及第二状态，用于在将所述解决方案从所述第一泵传送到所述第二采样回路的同时，通过所述第二泵推出所述第一采样回路中的所述解。

公开(公告)号：US08496819B2

公开(公告)日：2013-07-30

申请号：US12146912

申请日：2008-06-26

申请人： Katsutoshi Shimizu ,  Masahito Ito ,  Kimihiko Ishii ,  Yoshihiro Nagaoka ,  Kisaburo Deguchi ,  Takefumi Yokokura

发明人： Katsutoshi Shimizu ,  Masahito Ito ,  Kimihiko Ishii ,  Yoshihiro Nagaoka ,  Kisaburo Deguchi ,  Takefumi Yokokura

IPC分类号： B01D15/08 ,  G01N30/02 ,  C02F1/28

CPC分类号： B01D15/22 ,  B01J20/282 ,  B01J2220/82 ,  B01J2220/825 ,  G01N30/22 ,  G01N30/6047 ,  G01N30/6052 ,  G01N30/6069 ,  G01N2030/522 ,  G01N2030/528

摘要： Both a column having high-pressure resistance and capable of performing high-speed separation and analysis even with a small flow rate and a liquid chromatograph apparatus using the column are provided. A separation column according to the present invention has a monolithic rod being arranged in the center, being made of a porous material, and having a circular cross section, a filler layer arranged such that it encircles a circumferential surface of the monolithic rod, and a cylindrical support medium arranged outside the filler layer. The filler layer is formed by filling a tube-like gap between the monolithic rod and the support medium with particles or beads.

摘要翻译： 提供了具有高耐压性并且即使以小流量进行高速分离和分析的柱和使用该柱的液相色谱装置。 根据本发明的分离柱具有中心的整体式杆，由多孔材料制成，并且具有圆形横截面，填充层布置成使其围绕整体式杆的圆周表面，并且 圆柱形支撑介质布置在填充层外部。 通过在颗粒或珠粒之间填充整体棒和支撑介质之间的管状间隙来形成填充层。

公开(公告)号：US20110303599A1

公开(公告)日：2011-12-15

申请号：US13202722

申请日：2010-03-10

申请人： Norimasa Minamoto ,  Masahito Ito ,  Kisaburo Deguchi ,  Hiroyuki Tanai ,  Kiyotoshi Mori

发明人： Norimasa Minamoto ,  Masahito Ito ,  Kisaburo Deguchi ,  Hiroyuki Tanai ,  Kiyotoshi Mori

IPC分类号： B01D15/08 ,  B01D39/10

CPC分类号： G01N30/603

摘要： In a liquid chromatograph, the disulfide adsorption of iron atoms in a filter for a column is avoided and thus peak tailing in a chromatogram is prevented to thereby prevent a lowering in the sample recovery rate. A liquid chromatograph column comprising a hollow tubular column body, a bead-shaped packing material which is packed in the hollow part of the column body, a filter provided with a mesh which has a mesh size smaller than the particle diameter of the packing material, and a cap which presses the filter against the packing material to thereby enclose the packing material within the column body, characterized in that the main component of the filter is nickel or hastelloy.

摘要翻译： 在液相色谱仪中，避免了用于柱的过滤器中的铁原子的二硫键吸附，因此防止了色谱图中的峰值拖尾，从而防止了样品回收率的降低。 一种液相色谱柱，包括中空管状柱体，填充在柱体中空部分中的珠状包装材料，具有筛网尺寸小于包装材料粒径的筛网的过滤器， 以及将过滤器压靠在包装材料上的盖子，从而将包装材料包围在柱体内，其特征在于，过滤器的主要部件是镍或哈氏合金。

公开(公告)号：US20070199874A1

公开(公告)日：2007-08-30

申请号：US11700844

申请日：2007-02-01

申请人： Masahito Ito ,  Junkichi Miura ,  Kisaburo Deguchi

发明人： Masahito Ito ,  Junkichi Miura ,  Kisaburo Deguchi

IPC分类号： B01D15/08

CPC分类号： G01N30/463 ,  G01N30/461 ,  G01N30/7233 ,  G01N30/96 ,  B01D15/322 ,  B01D15/362 ,  B01D15/325 ,  B01D15/363

摘要： In a liquid chromatography apparatus, a separation column of intermediate stage is additionally connected between a separation column of first stage and a separation column of second stage. Preferably, a switching unit and a liquid feed unit for mixing and feeding a plurality of solutions are added to improve a separation capability. A three-dimensional liquid chromatography apparatus capable of avoiding the “solution interference” can be realized. Even a complex sample containing a hydrophilic component and a hydrophobic component in a mixed state can be separated and analyzed satisfactorily on-line.

摘要翻译： 在液相色谱装置中，中间级分离塔另外连接在第一级分离塔和第二级分离塔之间。 优选地，添加用于混合和供给多个溶液的切换单元和液体进料单元以提高分离能力。 可以实现能够避免“溶液干扰”的三维液相色谱仪。 即使是含有亲水性成分和混合状态的疏水性成分的复合物，也可以在线进行分离和分析。

公开(公告)号：US20060271310A1

公开(公告)日：2006-11-30

申请号：US11501750

申请日：2006-08-10

申请人： Masahito Ito ,  Kisaburo Deguchi

发明人： Masahito Ito ,  Kisaburo Deguchi

IPC分类号： G01N31/00

CPC分类号： G01N30/8624 ,  G01N30/8631 ,  G01N30/8679 ,  G01N2201/1293

摘要： A method for analyzing a multi-channel chromatogram is realized for accurately resolving overlapping peaks on a multi-channel chromatogram to permit analysis of the composition of a sample. First, components of overlapping peaks are specified. A data matrix Dij is determined, and compressed in the wavelength direction to obtain a data matrix vector Di. Next, the two-dimensional data is deconvoluted, final standard deviation s0 is registered, and the deconvoluted two-dimensional data is reconvoluted to a retention intensity matrix. A quantitative spectral intensity matrix is computed, components are identified, and the identified components are quantified. Subsequently, an eigenvalue problem is solved, and the number n of components is estimated. The deconvolution and computation of the eigenvalue problem are iteratively executed until the peaks are isolated. Then, elution profiles are calculated, the components are identified, and the identified components are quantified.

摘要翻译： 实现了分析多通道色谱图的方法，用于准确地分辨多通道色谱图上的重叠峰，以便分析样品的组成。 首先，指定重叠峰的分量。 确定数据矩阵Dij，并在波长方向上压缩以获得数据矩阵向量Di。 接下来，对二维数据进行解卷积，记录最终标准偏差s0，并将解卷积的二维数据重新卷积到保留强度矩阵。 计算定量光谱强度矩阵，识别组件，并对所识别的组分进行量化。 随后，解决了特征值问题，并且估计了分量的数量n。 迭代执行反褶积和特征值问题的计算，直到峰被隔离。 然后，计算洗脱曲线，识别组分，并对所鉴定的组分进行定量。

公开(公告)号：US06393368B1

公开(公告)日：2002-05-21

申请号：US08811486

申请日：1997-03-05

申请人： Masahito Ito ,  Kisaburo Deguchi

发明人： Masahito Ito ,  Kisaburo Deguchi

IPC分类号： G01N3000

CPC分类号： G01N30/8624 ,  G01N30/8631 ,  G01N30/8679 ,  G01N2201/1293

摘要： A method for analyzing a multichannel chromatogram is realized for accurately resolving overlapping peaks on a multichannel chromatogram to permit analysis of the composition of a sample. First, components of overlapping peaks are specified. A data matrix Dij is determined, and compressed in the wavelength direction to obtain a data matrix vector Di. By compressing three-dimensional data in the wavelength direction, noise components cancel with each other to obtain noise free two-dimensional data Di&bgr;. Next, the two-dimensional data is deconvoluted, final standard deviation s0 is registered, and the deconvoluted two-dimensional data is reconvoluted to a retention intensity matrix. A quantitative spectral intensity matrix is computed, components are identified, and the identified components are quantified. The quantified data is displayed on a screen. Since noise components cancel with each other, it is possible to realize an analyzing method which is capable of accurately resolving and analyzing the components. Also, overlapping peaks are specified, and a data matrix D is determined. Subsequently, an eigenvalue problem is solved, and the number n of components is estimated. The deconvolution and computation of the eigenvalue problem are iteratively executed until the peaks are isolated. Then, elution profiles are calculated, the components are identified, and the identified components are quantified.