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公开(公告)号：US5304483A
公开(公告)日：1994-04-19
申请号：US493369
申请日：1990-03-14
摘要： Stable, long-term cultures of animal cells are accomplished by calculating the ratio of lactic acid formed by the cells to the glucose consumed by the cells (the L/G ratio) and controlling the cultivation conditions to maintain the L/G ratio between the limits of from greater than 0 to 0.6. When the ratio is outside the desired range, the dissolved oxygen concentration in the medium is increased and/or the glucose concentration in the medium is decreased.
摘要翻译： 通过计算由细胞形成的乳酸与细胞消耗的葡萄糖的比例（L / G比），并控制培养条件以维持L / G比例的L / G比，来实现动物细胞的稳定的长期培养 极限从0到0.6。 当比例在所需范围之外时，介质中的溶解氧浓度增加和/或介质中的葡萄糖浓度降低。
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2.发明授权Apparatus containing a septum which impedes cell permeation for cell culture and method of use 失效含有阻碍细胞培养的细胞渗透的隔膜和使用方法的装置
公开(公告)号：US5151362A
公开(公告)日：1992-09-29
申请号：US505326
申请日：1990-04-05
CPC分类号： C12M29/04 , C12M29/10 , C12M41/00 , Y10S435/813
摘要： By culturing cells in an apparatus containing a first zone in which a cell suspended medium is present and a second zone in which liquid containing a physiological saline solution and/or nutrient sources is present, the first zone being separated from the second zone by a septum with characteristics, such as pores size or electrostatic charges, which impedes the cells from freely permeating through, while allowing at least one of nutrient sources, cell wastes or the product to move between the first zone and the second zone via the septum to proliferate cells in the first zone, wherein a flow rate via the septum is controlled in such a manner that the cell count moved from the first zone into the second zone via the septum does not exceed the cell count increased by cell growth in the first zone. Thus, cell culture at a high cell density can be achieved in a large scale.
摘要翻译： 通过在包含存在细胞悬浮介质的第一区域和存在含有生理盐水溶液和/或营养源的液体的第二区域的装置中培养细胞，第一区域通过隔膜与第二区域分离 具有阻止细胞自由渗透的特征，例如孔径或静电电荷，同时允许营养源，细胞废物或产物中的至少一种通过隔膜在第一区和第二区之间移动以增殖细胞 在第一区域中，其中以通过隔膜从第一区域移动到第二区域的方式控制经由隔膜的流速不超过由第一区域中的细胞生长增加的细胞计数。 因此，可以大规模地实现高细胞密度的细胞培养。
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公开(公告)号：US5414072A
公开(公告)日：1995-05-09
申请号：US588783
申请日：1990-09-27
申请人： Tadashi Ishibashi , Hiroaki Kezuka , Saeko Yoshino , Norio Shimizu , Motoyuki Tsuda , Shuji Imazeki
发明人： Tadashi Ishibashi , Hiroaki Kezuka , Saeko Yoshino , Norio Shimizu , Motoyuki Tsuda , Shuji Imazeki
CPC分类号： C07K16/18 , C07K2317/73
摘要： The invention relates to a monoclonal antibody which binds to octopus rhodopsin and octopus metarhodopsin wherein said monoclonal antibody binds with higher affinity to metarhodopsin than to rhodopsin and does not inhibit rhodopsin-metarhodopsin interconversion upon exposure to blue or red light. The monoclonal antibody binds to a peptide having the sequence Val-Ile-Gly-Arg-Pro-Met-Ala-Ala-Ser-Lys-Lys-Met-Ser. The photochemical reaction of rhodopsin thin layers may be detected using the anti-rhodopsin monoclonal antibodies of the invention.
摘要翻译： 本发明涉及结合章鱼视紫红质和章鱼metarhodopsin的单克隆抗体，其中所述单克隆抗体以比视紫红质更高的亲和性结合对视紫质，并且在暴露于蓝色或红色光时不抑制视紫红质 - 视紫红质相互转化。 单克隆抗体结合具有Val-Ile-Gly-Arg-Pro-Met-Ala-Ala-Ser-Lys-Lys-Met-Ser序列的肽。 可以使用本发明的抗视紫红质单克隆抗体检测视紫质薄层的光化学反应。
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公开(公告)号：US5328843A
公开(公告)日：1994-07-12
申请号：US660875
申请日：1991-02-26
申请人： Jun Fukuda , Hideo Kawaguchi , Takejiro Ushiroda , Norio Shimizu , Kazuo Sato
发明人： Jun Fukuda , Hideo Kawaguchi , Takejiro Ushiroda , Norio Shimizu , Kazuo Sato
CPC分类号： B01L3/0293 , B01L3/5027 , C12M33/00 , B01L2300/046 , B01L2300/0816 , B01L2300/0822 , B01L2400/0406
摘要： Apertures in a definite shape having a definite width and length are provided on one surface of a plate material having a definite thickness. One end of the apertures is connected with the other surface of the plate material and at the same time, is opened. Another end of the apertures is connected with the plate material on one surface and is also opened toward the other surface of a culture substrate. The plate material is closely contacted to one surface of the culture substrate for allocating nerve cells and observing growth of the neurites. Then, the cell suspension is supplied to the apertures. The cells in the suspension are allocated on the culture substrate in response to the apertures by centrifugation or spontaneous sedimentation. The plate material is then withdrawn.
摘要翻译： 在具有确定厚度的板材的一个表面上设置具有确定宽度和长度的确定形状的孔。 孔的一端与板材的另一面连接，同时打开。 孔的另一端在一个表面上与板材连接，并且朝向培养基底的另一表面打开。 板材与培养基质的一个表面紧密接触，用于分配神经细胞并观察神经突生长。 然后，将细胞悬浮液提供给孔。 悬浮液中的细胞通过离心或自发沉降响应于孔而分配在培养基底上。 然后取出板材。
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公开(公告)号：US07045942B2
公开(公告)日：2006-05-16
申请号：US11142135
申请日：2005-06-01
申请人： Norio Shimizu , Fumiaki Nihei , Toshio Uchikawa
发明人： Norio Shimizu , Fumiaki Nihei , Toshio Uchikawa
IPC分类号： H01J29/81
CPC分类号： H01J29/07 , H01J29/861 , H01J2229/0788 , H01J2229/0794 , H01J2229/862
摘要： The radius of curvature of the outer surface of a panel is 10,000 mm or more, and a shadow mask is made of a material containing 95% or more of iron. A sagging amount change curve along a curve C1 on the surface of the shadow mask, which a plane passing through a center P0 of a useful area of the shadow mask and parallel to a tube axis and a major axis crosses, satisfies a particular Condition 1. Assuming that an intersection between the curve C1 and a useful area end of the shadow mask is a major axis end PL, a distance from the center P0 to the major axis end PL along a major axis is W, and a point on the curve C1 away from the center P0 by ⅔×W in the major axis direction is P1, a sagging amount change curve along a curve C2 on the surface of the shadow mask, which a plane passing through the point P1 and parallel to the tube axis and the minor axis crosses, satisfies a particular Condition 2. Consequently, a color picture tube can be realized, which has satisfactory visibility, and less degradation in color purity caused by doming while having a shadow mask made of an inexpensive material with satisfactory moldability.
摘要翻译： 面板的外表面的曲率半径为10,000mm以上，荫罩由含有95％以上的铁的材料构成。 沿着荫罩表面的曲线C 1的下垂量变化曲线，其通过荫罩的有用区域的中心P 0并平行于管轴和长轴交叉的平面满足特定的 条件1.假设曲线C 1和荫罩的有用区域端之间的交点是长轴端PL，则沿着长轴的中心P 0到长轴端PL的距离为W，并且 在长轴方向上离开中心P 0×2/3×W的曲线C 1上的点是P 1，沿着荫罩表面上的曲线C 2的下垂量变化曲线，通过点 P 1并且平行于管轴和短轴交叉，满足特定的条件2.因此，可以实现具有令人满意的可视性的彩色显像管，并且具有由具有荫罩制成的较少的由拱形引起的色纯度降低 的廉价材料，具有令人满意的成型性。
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公开(公告)号：US20050269930A1
公开(公告)日：2005-12-08
申请号：US11142135
申请日：2005-06-01
申请人： Norio Shimizu , Fumiaki Nihei , Toshio Uchikawa
发明人： Norio Shimizu , Fumiaki Nihei , Toshio Uchikawa
CPC分类号： H01J29/07 , H01J29/861 , H01J2229/0788 , H01J2229/0794 , H01J2229/862
摘要： The radius of curvature of the outer surface of a panel is 10,000 mm or more, and a shadow mask is made of a material containing 95% or more of iron. A sagging amount change curve along a curve C1 on the surface of the shadow mask, which a plane passing through a center P0 of a useful area of the shadow mask and parallel to a tube axis and a major axis crosses, satisfies a particular Condition 1. Assuming that an intersection between the curve C1 and a useful area end of the shadow mask is a major axis end PL, a distance from the center P0 to the major axis end PL along a major axis is W, and a point on the curve C1 away from the center P0 by ⅔×W in the major axis direction is P1, a sagging amount change curve along a curve C2 on the surface of the shadow mask, which a plane passing through the point P1 and parallel to the tube axis and the minor axis crosses, satisfies a particular Condition 2. Consequently, a color picture tube can be realized, which has satisfactory visibility, and less degradation in color purity caused by doming while having a shadow mask made of an inexpensive material with satisfactory moldability.
摘要翻译： 面板的外表面的曲率半径为10,000mm以上，荫罩由含有95％以上的铁的材料构成。 沿着荫罩表面的曲线C 1的下垂量变化曲线，其通过荫罩的有用区域的中心P 0并平行于管轴和长轴交叉的平面满足特定的 条件1.假设曲线C 1和荫罩的有用区域端之间的交点是长轴端PL，则沿着长轴的中心P 0到长轴端PL的距离为W，并且 在长轴方向上离开中心P 0×2/3×W的曲线C 1上的点是P 1，沿着荫罩表面上的曲线C 2的下垂量变化曲线，通过点 P 1并且平行于管轴和短轴交叉，满足特定的条件2.因此，可以实现具有令人满意的可视性的彩色显像管，并且具有由具有荫罩制成的较少的由拱形引起的色纯度降低 的廉价材料，具有令人满意的成型性。
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公开(公告)号：US20050007007A1
公开(公告)日：2005-01-13
申请号：US10909317
申请日：2004-08-03
申请人： Norio Shimizu , Toshio Uchikawa
发明人： Norio Shimizu , Toshio Uchikawa
CPC分类号： H01J29/861 , H01J29/07 , H01J2229/0788 , H01J2229/862
摘要： The mask body satisfies the following conditions, (ZMD−ZMH)/LMS≧0.020, and 0.065≦ZMD/LMD≦0.095 where in a substantially rectangular effective region with a predetermined curvature of the mask body, LMD is a distance between a central part and a diagonal-axis end of the effective region, LMS is a distance between a horizontal-axis end and the diagonal-axis end of the effective region, ZMD is a height difference between the central part and the diagonal-axis end in a direction of the tube axis, and ZMH is a height difference between the central part and the horizontal-axis end in the direction of the tube axis. With this structure, the mechanical strength of the mask body can be improved, and deformation of the mask body in a fabrication process and due to external shock can be prevented.
摘要翻译： 掩模体满足以下条件：（ZMD-ZMH）/ LMS> = 0.020，并且0.065≤ZMD/ LMD <= 0.095其中在具有掩模体的预定曲率的基本矩形的有效区域中，LMD是 有效区域的中心部分和对角轴端部，LMS是有效区域的水平轴端与对角轴端之间的距离，ZMD是中心部分与对角轴端部之间的高度差 在管轴的方向上，ZMH是管轴方向上的中心部和水平轴端之间的高度差。 通过这种结构，可以提高面罩主体的机械强度，并且可以防止制造工艺中的面罩主体和外部冲击时的变形。
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8.发明授权Color cathode ray tube having particular effective inner panel surface and shadow mask effective surface shapes 失效彩色阴极射线管具有特别有效的内表面和荫罩有效的表面形状公开(公告)号：US06333594B1
公开(公告)日：2001-12-25
申请号：US09220342
申请日：1998-12-24
IPC分类号： H01J2910
CPC分类号： H01J29/861 , H01J2229/862
摘要： Provided is a color cathode ray tube, which comprises a panel including a substantially rectangular effective portion, of which the outer surface is a flat surface or a slightly curved surface, and a shadow mask having an effective surface opposite the inner surface of the effective portion of the panel. In this color cathode ray tube, the inner surface of the effective portion of the panel is a curved surface having a substantially infinite curvature radius RXp1 in the major-axis direction on the major axis thereof near the center, a predetermined curvature radius RXp2 in the major-axis direction near peripheral portions on the major axis, and another predetermined curvature radius in the minor-axis direction on the minor axis thereof. This color cathode ray tube, with its panel having the substantially flat outer surface, enjoys improved visibility and higher strength for the maintenance of the curved surface of the shadow mask.
摘要翻译： 本发明提供一种彩色阴极射线管，其特征在于，具有包括大致矩形的有效部分的面板，所述面板的外表面为平坦面或微弯曲面，荫罩具有与有效部分的内表面相对的有效面 的面板。 在该彩色阴极射线管中，面板有效部分的内表面是在中心附近的长轴上的长轴方向上具有大致无限曲率半径RXp1的曲面，在中心的长轴上具有预定的曲率半径RXp2 在长轴上的周边部分附近的长轴方向和短轴上的短轴方向上的另一个预定的曲率半径。 该彩色阴极射线管具有基本上平坦的外表面，具有改进的可视性和更高的强度，用于维护荫罩的曲面。
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公开(公告)号：US06323589B1
公开(公告)日：2001-11-27
申请号：US09255697
申请日：1999-02-23
申请人： Masatsugu Inoue , Norio Shimizu , Tadashi Ite , Nobuhiko Akoh
发明人： Masatsugu Inoue , Norio Shimizu , Tadashi Ite , Nobuhiko Akoh
IPC分类号： H01J2980
CPC分类号： H01J29/07 , H01J2229/0772
摘要： A color cathode ray tube has a shadow mask. The shadow mask is composed of an almost rectangular mask body and an almost rectangular mask frame. The mask body has an effective surface facing a fluorescent screen, a non-aperture section surrounding the effective surface, and a bent skirt section formed on the periphery of the non-aperture section. The mask frame has a sidewall section provided on the skirt section of the mask body. In the shadow mask of such a configuration, a step section having a step in the direction perpendicular to the skirt section of the mask body is formed. With this configuration, the strength with which the curved surface of the effective surface of the shadow mask is enhanced, which prevents the color purity from deteriorating due to deformation and facilitates the formation of the shadow mask.
摘要翻译： 彩色阴极射线管具有荫罩。 荫罩由几乎矩形的荫罩体和几乎矩形的荫罩框组成。 面罩主体具有面向荧光屏的有效表面，围绕有效表面的非开口部分，以及形成在非开口部分的周边上的弯曲裙部。 面罩框架具有设置在面罩主体的裙部上的侧壁部分。 在这种构造的荫罩中，形成有与荫罩主体的裙部垂直的方向的台阶部。 通过这种结构，荫罩的有效面的曲面的强度提高，防止色变因变形而劣化，容易形成荫罩。
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10.发明授权
公开(公告)号：US5672934A
公开(公告)日：1997-09-30
申请号：US502174
申请日：1995-07-13
申请人： Shinji Ohama , Norio Shimizu , Takashi Murai , Ichiro Saotome , Masatsugu Inoue , Kumio Fukuda
发明人： Shinji Ohama , Norio Shimizu , Takashi Murai , Ichiro Saotome , Masatsugu Inoue , Kumio Fukuda
CPC分类号： H01J29/076 , H01J29/07 , H01J2229/0788 , H01J2229/0794
摘要： A color-cathode ray tube having a shadow mask with improved aperture arrays. The aperture arrays are spaced at appropriate intervals and are curved to suppress local doming of the effective part of the shadow mask and to prevent electron-beam mislanding from occurring on a phosphor screen. The distance between any two adjacent apertures is expressed as: PH(N)=A+BN.sup.2 +CN.sup.4, where PH(N) is the distance between the (N-1)th and Nth arrays and A, B, and C are fourth-degree functions of a Y-coordinate. The apertures along a long side of the effective part of the shadow mask in a section extending for one-fourth of a width of the effective part of the shadow mask from a short side of the effective part of the shadow mask are inclined in an opposite direction to the apertures within the section and located one-third of a height of the effective part of the shadow mask from the long side.
摘要翻译： 一种彩色阴极射线管，其具有改进的孔阵列的荫罩。 孔阵列以适当的间隔间隔开并弯曲以抑制荫罩的有效部分的局部隆起并防止在荧光屏上发生电子束偏移。 任何两个相邻孔之间的距离表示为：PH（N）= A + BN2 + CN4，其中PH（N）是第（N-1）和第N阵列之间的距离，A，B和C是第四 Y坐标的函数。 沿着荫罩的有效部分的长边沿从荫罩的有效部分的短边延伸到荫罩的有效部分的宽度的四分之一的部分中的孔相对倾斜 方向到该部分内的孔，并且从长边位于荫罩的有效部分的高度的三分之一处。
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