Methods and compositions for modulating telomerase reverse transcriptase (TERT) expression
    6.
    发明申请
    Methods and compositions for modulating telomerase reverse transcriptase (TERT) expression 审中-公开
    调节端粒酶逆转录酶(TERT)表达的方法和组合物

    公开(公告)号:US20080058277A1

    公开(公告)日:2008-03-06

    申请号:US11713556

    申请日:2007-02-28

    IPC分类号: A61K48/00 A61P43/00

    摘要: Methods and compositions are provided for modulating, and generally upregulating, the expression of telomerase reverse transcriptase (TERT) by blocking repression of TERT transcription, e.g., by inhibiting binding of repressor factor to a Site C repressor binding site located in the TERT minimal promoter. The subject methods and compositions find use in a variety of different applications, including the immortalization of cells, the production of reagents for use in life science research, therapeutic applications; therapeutic agent screening applications; and the like. In further describing the subject invention, the methods and compositions of the invention are described first in greater detail, followed by a review of the various applications in which the subject invention finds use.

    摘要翻译: 提供方法和组合物用于通过阻止TERT转录的抑制来调节和通常上调端粒酶逆转录酶(TERT)的表达,例如通过抑制阻遏物因子与位于TERT最小启动子中的位点C阻遏物结合位点的结合。 主题方法和组合物可用于各种不同的应用,包括细胞的永生化,用于生命科学研究的试剂的生产,治疗应用; 治疗剂筛选应用; 等等。 在进一步描述本发明时,首先更详细地描述本发明的方法和组合物,随后对本发明所使用的各种应用进行回顾。

    Methods and compositions for modulating telomerase reverse transcriptase (TERT) expression
    8.
    发明申请
    Methods and compositions for modulating telomerase reverse transcriptase (TERT) expression 审中-公开
    调节端粒酶逆转录酶(TERT)表达的方法和组合物

    公开(公告)号:US20080176223A1

    公开(公告)日:2008-07-24

    申请号:US11385250

    申请日:2006-03-20

    摘要: Nucleic acid compositions comprising a region and a TERT minimal promoter activator binding site that acts to activate transcription of the telomerase reverse transcriptase (TERT) coding sequence, as well as vectors and constructs including the same, are provided. Also provided are methods of modulating, e.g., inhibiting, the TERT transcription activation activity of the subject TERT activator binding site regions in order to regulate, e.g., repress, telomerase expression, which methods find use in a variety of different applications, including the therapeutic applications and the like. In addition, methods of screening for agents that modulate the TERT transcription activating activity of the subject TERT activator sites are provided. The subject invention finds use in, among other applications, the regulation of TERT expression.

    摘要翻译: 提供了包含作用于激活端粒酶逆转录酶(TERT)编码序列的转录的区域和TERT最小启动子激活子结合位点的核酸组合,以及包含其的载体和构建体。 还提供了调节,例如抑制受试者TERT激活物结合位点区域的TERT转录活化活性以调节例如抑制端粒酶表达的方法,该方法可用于多种不同应用,包括治疗 应用程序等。 此外,提供筛选调节受试者TERT激活剂位点的TERT转录激活活性的试剂的方法。 除了其他应用之外,本发明可用于调节TERT表达。

    Methods and compositions for modulating telomerase reverse transcriptase (TERT) expression
    9.
    发明申请
    Methods and compositions for modulating telomerase reverse transcriptase (TERT) expression 审中-公开
    调节端粒酶逆转录酶(TERT)表达的方法和组合物

    公开(公告)号:US20050118149A1

    公开(公告)日:2005-06-02

    申请号:US10863056

    申请日:2004-06-07

    摘要: Methods and compositions are provided for modulating, and generally upregulating, the expression of telomerase reverse transcriptase (TERT) by blocking repression of TERT transcription, e.g., by inhibiting binding of repressor factor to a Site C repressor binding site located in the TERT minimal promoter. The subject methods and compositions find use in a variety of different applications, including the immortalization of cells, the production of reagents for use in life science research, therapeutic applications; therapeutic agent screening applications; and the like. In further describing the subject invention, the methods and compositions of the invention are described first in greater detail, followed by a review of the various applications in which the subject invention finds use.

    摘要翻译: 提供方法和组合物用于通过阻止TERT转录的抑制来调节和通常上调端粒酶逆转录酶(TERT)的表达,例如通过抑制阻遏物因子与位于TERT最小启动子中的位点C阻遏物结合位点的结合。 主题方法和组合物可用于各种不同的应用,包括细胞的永生化,用于生命科学研究的试剂的生产,治疗应用; 治疗剂筛选应用; 等等。 在进一步描述本发明时,首先更详细地描述本发明的方法和组合物,随后对本发明所使用的各种应用进行回顾。