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1.发明授权Oxidase gene and method for producing 3-indole-pyruvic acid by utilizing the gene 有权氧化酶基因及利用该基因产生3-吲哚 - 丙酮酸的方法公开(公告)号：US08394940B2
公开(公告)日：2013-03-12
申请号：US12711719
申请日：2010-02-24
申请人： Yasuaki Takakura , Seiichi Hara , Toshiki Taba , Shunichi Suzuki , Masakazu Sugiyama , Kunihiko Watanabe , Kenzo Yokozeki
发明人： Yasuaki Takakura , Seiichi Hara , Toshiki Taba , Shunichi Suzuki , Masakazu Sugiyama , Kunihiko Watanabe , Kenzo Yokozeki
IPC分类号： C07H21/04
CPC分类号： C12P17/10 , C12N9/0022
摘要： It is an object of the present invention to provide a procedure for realizing inexpensive and simple production of 3-indole-pyruvic acid. A transformant is made using a polynucleotide having a specific nucleotide sequence encoding a protein having an oxidase activity, and oxidase is generated by culturing the transformant in a medium to accumulate the oxidase in the medium and/or the transformant. Further, tryptophan is converted into 3-indole-pyruvic acid in the presence of the transformant and/or a culture thereof to produce 3-indole-pyruvic acid.
摘要翻译： 本发明的目的是提供一种实现廉价且简单的3-吲哚 - 丙酮酸生产的方法。 使用具有编码具有氧化酶活性的蛋白质的特异性核苷酸序列的多核苷酸制备转化体，通过在培养基中培养转化体以在培养基和/或转化体中积累氧化酶而产生氧化酶。 此外，在转化体和/或其培养物存在下，将色氨酸转化为3-吲哚 - 丙酮酸，生成3-吲哚 - 丙酮酸。
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公开(公告)号：US08273556B2
公开(公告)日：2012-09-25
申请号：US13250359
申请日：2011-09-30
摘要： 4-(Indol-3-ylmethyl)-4-hydroxy-2-oxoglutarate, which is useful as an intermediate in the synthesis of monatin, may be synthesized from indole pyruvic acid and pyruvic acid (and/or oxaloacetic acid) by using a novel aldolase derived from the genus Pseudomonas, Erwinia, Flavobacterium, or Xanthomonas.
摘要翻译： 可用作合成莫纳甜的中间体的4-（吲哚-3-基甲基）-4-羟基-2-氧戊二酸，可以由吲哚丙酮酸和丙酮酸（和/或草酰乙酸）通过使用 衍生自假单胞菌属，欧文氏菌属，黄杆菌属或黄单胞菌属的新型醛缩酶。
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公开(公告)号：US08043836B2
公开(公告)日：2011-10-25
申请号：US11576421
申请日：2005-09-29
IPC分类号： C12P1/00 , C12P13/04 , C12P13/22 , C12P17/10 , C07D209/18
CPC分类号： C12P13/227 , C12P13/04 , C12R1/01 , C12R1/185 , C12R1/19
摘要： The present invention is to provide an industrially advantageous method for producing an amino acid derivative. Provided is a method for producing an amino acid derivative including contacting a microorganism and/or an enzyme with a hydroxyimino acid represented by the following general formula (I): wherein R1 represents an optionally substituted predetermined hydrocarbon group; R2 represents a C1 to C3 alkyl group or a hydrogen atom; and n is 0 or 1, to produce an amino acid derivative represented by the following general formula (III): wherein R1 and n have the same meanings as those of R1 and n in the general formula (I), wherein the microorganism and/or the enzyme is capable of catalyzing the reaction.
摘要翻译： 本发明提供一种工业上有利的氨基酸衍生物的制备方法。 本发明提供一种生产氨基酸衍生物的方法，其包括使微生物和/或酶与由以下通式（I）表示的肟基酸接触：其中R1表示任选取代的预定烃基; R2表示C1〜C3烷基或氢原子; 和n为0或1，以产生由以下通式（III）表示的氨基酸衍生物：其中R 1和n与通式（I）中的R 1和n的含义相同，其中微生物和/ 或者酶能够催化反应。
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公开(公告)号：US20100317068A1
公开(公告)日：2010-12-16
申请号：US12836984
申请日：2010-07-15
申请人： Shinji Kuroda , Masakazu Sugiyama , Kunihiko Watanabe , Shunichi Suzuki , Kenzo Yokozeki , Tatsuki Kashiwagi
发明人： Shinji Kuroda , Masakazu Sugiyama , Kunihiko Watanabe , Shunichi Suzuki , Kenzo Yokozeki , Tatsuki Kashiwagi
CPC分类号： C12N9/1029 , C12P13/04
摘要： The present invention describes a method for generating a serine derivative and an optically active isomer thereof by a convenient technique, and an enzyme and the like useful in the method. In the presence of the following protein (A) and/or (B) having an enzymatic activity, an α-amino acid is reacted with an aldehyde to form a serine derivative: (A) a protein comprising the amino acid sequence of SEQ ID NO:5, and (B) a protein comprising an amino acid sequence of SEQ ID NO: 5, but which includes substitution, deletion, insertion and addition of one or more amino acids and is able to catalyze the reaction to form the serine derivative.
摘要翻译： 本发明描述了通过方便的技术产生丝氨酸衍生物和光学活性异构体的方法，以及在该方法中有用的酶等。 在具有酶活性的下列蛋白质（A）和/或（B）的存在下，α-氨基酸与醛反应形成丝氨酸衍生物：（A）包含SEQ ID NO：1的氨基酸序列的蛋白质 （B）包含SEQ ID NO：5的氨基酸序列但包括取代，缺失，插入和加入一个或多个氨基酸的蛋白质，并且能够催化该反应以形成丝氨酸衍生物 。
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5.发明申请公开(公告)号：US20100255548A1
公开(公告)日：2010-10-07
申请号：US12758433
申请日：2010-04-12
IPC分类号： C12P13/04
CPC分类号： C12N9/1096 , C12P17/10
摘要： A D-aminotransferase can be modified so as to efficiently produce (2R,4R)-monatin having high sweetness intensity from 4-(indol-3-ylmethyl)-4-hydroxy-2-oxoglutaric acid by substituting an amino acid at least at one of positions (positions 100, 180 to 183, 243 and 244) involved in efficiently producing the (2R,4R)-monatin in an amino acid sequence of a wild-type D-aminotransferase represented in SEQ ID NO:2.
摘要翻译： 可以修饰D-氨基转移酶，以便通过用氨基酸至少在4-（吲哚-3-基甲基）-4-羟基-2-氧代戊二酸代替氨基酸，有效地产生具有高甜度强度的（2R，4R） 在SEQ ID NO：2所示的野生型D-氨基转移酶的氨基酸序列中有效产生（2R，4R） - 单抗的位置（位置100,180〜183,243和244）之一。
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公开(公告)号：US07745182B2
公开(公告)日：2010-06-29
申请号：US12345922
申请日：2008-12-30
CPC分类号： C12P13/04 , C12N9/1014
摘要： A method for producing β-hydroxy amino acid and its optically-active isomer is provided. The β-hydroxy amino acid is produced by reacting a predetermined D-α-amino acid and 5,10-methylene tetrahydrofolic acid in the presence of an enzyme derived from a microorganism belonging to the genera Paracoccus, Aminobacter, or Ensifer.
摘要翻译： 提供了一种生产β-羟基氨基酸及其光学活性异构体的方法。 β-羟基氨基酸通过使预定的D-α-氨基酸和5,10-亚甲基四氢叶酸在衍生自属于副球菌属，氨基杆菌属或Ensifer属的微生物的酶存在下反应来制备。
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公开(公告)号：US20090229880A1
公开(公告)日：2009-09-17
申请号：US11885152
申请日：2006-03-31
申请人： Kunihiko Watanabe
发明人： Kunihiko Watanabe
摘要： One aspect of the present invention can include a conductor to be installed on a vehicle for high current use including a stranded copper wire connected to an end portion of a single-core aluminum cable, an intermediary conductor made of copper is connected to the stranded copper wire and an end face of a core of the single-core aluminum cable is cold welded connected to an end face of a welding shaft formed on the intermediary conductor having approximately a same diameter as the core of the single-core aluminum cable.
摘要翻译： 本发明的一个方面可以包括安装在用于大电流的车辆上的导体，包括连接到单芯铝电缆的端部的绞合铜线，由铜制成的中间导体连接到绞合的铜 电线和单芯铝电缆的芯的端面被冷焊接连接到形成在中间导体上的焊接轴的端面，其具有与单芯铝电缆的芯大致相同的直径。
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公开(公告)号：US07556947B2
公开(公告)日：2009-07-07
申请号：US12345887
申请日：2008-12-30
CPC分类号： C12P13/04 , C12N9/1014
摘要： A method for producing β-hydroxy amino acid and its optically-active isomer is provided. The β-hydroxy amino acid is produced by reacting a predetermined D-α-amino acid and 5,10-methylene tetrahydrofolic acid in the presence of an enzyme derived from a microorganism belonging to the genera Paracoccus, Aminobacter, or Ensifer.
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公开(公告)号：US07351569B2
公开(公告)日：2008-04-01
申请号：US11066542
申请日：2005-02-28
摘要： 4-(Indol-3-ylmethyl)-4-hydroxy-2-oxoglutarate, which is useful as an intermediate in the synthesis of monatin, may be synthesized from indole pyruvic acid and pyruvic acid (and/or oxaloacetic acid) by using a novel aldolase derived from the genus Pseudomonas, Erwinia, Flavobacterium, or Xanthomonas.
摘要翻译： 可用作合成莫纳甜的中间体的4-（吲哚-3-基甲基）-4-羟基-2-氧戊二酸，可以由吲哚丙酮酸和丙酮酸（和/或草酰乙酸）通过使用 衍生自假单胞菌属，欧文氏菌属，黄杆菌属或黄单胞菌属的新型醛缩酶。
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公开(公告)号：US07297800B2
公开(公告)日：2007-11-20
申请号：US10876468
申请日：2004-06-28
申请人： Masakazu Sugiyama , Kunihiko Watanabe , Nao Funakoshi , Yusuke Amino , Shigeru Kawahara , Tadashi Takemoto
发明人： Masakazu Sugiyama , Kunihiko Watanabe , Nao Funakoshi , Yusuke Amino , Shigeru Kawahara , Tadashi Takemoto
IPC分类号： C07D209/00 , C12P17/00
摘要： The present invention relates to a process for producing efficiently glutamic acid derivatives (including salts thereof) such as monatin by converting a substituted α-keto acid of formula (1) into a glutamic acid derivative of formula (2) in the presence of an enzyme catalyzing conversion of the same
摘要翻译： 本发明涉及通过在式（2）的存在下将式（1）的取代的α-酮酸转化为式（2）的谷氨酸衍生物而有效地生产谷氨酸衍生物（包括其盐）的莫纳甜的方法 催化其转化
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