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    Enzymes with aminopeptidase activity
    7.
    发明授权
    Enzymes with aminopeptidase activity 失效
    具有氨基肽酶活性的酶

    公开(公告)号：US06413559B1

    公开(公告)日：2002-07-02

    申请号：US09718709

    申请日：2000-11-22

    申请人： Markus Sakari Kauppinen ,  Joan Qi Si ,  Tina Spendler ,  Claus Dambmann ,  Torben Halkier ,  Peter Rahbek Østergaard ,  Shamkant Anant Patkar ,  Kim Hansen

    发明人： Markus Sakari Kauppinen ,  Joan Qi Si ,  Tina Spendler ,  Claus Dambmann ,  Torben Halkier ,  Peter Rahbek Østergaard ,  Shamkant Anant Patkar ,  Kim Hansen

    IPC分类号： A21D200

    CPC分类号： C12N9/48 ,  A21D8/042

    摘要： The present invention relates to a 35 kDa enzyme exhibiting aminopeptidase activity which is derived from a fungal microorganism, a DNA construct comprising a DNA sequence encoding said enzyme, a recombinant expression vector comprising said DNA construct, and a cell comprising said DNA sequence. The present invention also provides a method for producing said enzyme exhibiting aminopeptidase activity, and an enzyme preparation comprising said enzyme, a bread-improving or dough-improving composition comprising the aminopeptidase of the invention. Finally the invention relates to the use of said enzyme exhibiting aminopeptidase activity or enzyme preparations or compositions thereof.

    摘要翻译： 本发明涉及一种表现出来自真菌微生物的氨基肽酶活性的35kDa酶，包含编码所述酶的DNA序列的DNA构建体，包含所述DNA构建体的重组表达载体和包含所述DNA序列的细胞。 本发明还提供了生产表现出氨基肽酶活性的酶的方法，以及包含所述酶的酶制剂，包含本发明的氨基肽酶的面包改良组合物或面团改良组合物。 最后，本发明涉及所述显示氨基肽酶活性的酶或酶制剂或其组合物的用途。

    Method for preparing modified polypeptides
    9.
    发明申请
    Method for preparing modified polypeptides 审中-公开
    制备修饰多肽的方法

    公开(公告)号：US20090088559A1

    公开(公告)日：2009-04-02

    申请号：US12011979

    申请日：2008-01-30

    申请人： Torben Halkier ,  Anders Hjelholt Pedersen ,  Jens Sigurd Okkels ,  Kim Vilbour Andersen

    发明人： Torben Halkier ,  Anders Hjelholt Pedersen ,  Jens Sigurd Okkels ,  Kim Vilbour Andersen

    IPC分类号： C07K14/00

    CPC分类号： C12N15/1058 ,  C07K1/107 ,  C07K14/31 ,  C07K19/00 ,  C12N15/1034

    摘要： Methods for producing polypeptide with altered immunogenicity or improved stability properties are disclosed. The methods involve a) expressing a diversified population of nucleotide sequences encoding a polypeptide of interest, b) screening the polypeptides expressed in step a) for function, immunogenicity and/or stability, c) selecting functional polypeptides having altered immunogenicity and/or increased stability, e.g. functional in vivo half-life as compared to the polypeptide of interest, and d) optionally subjecting the nucleotide sequence encoding the polypeptide selected in step c) to one or more repeated cycles of steps a)-c). In a further step the expressed polypeptides of step a) or c) can be conjugated to at least one non-polypeptide moiety.

    摘要翻译： 公开了产生具有改变的免疫原性或改善的稳定性的多肽的方法。 所述方法包括：a）表达编码感兴趣多肽的核苷酸序列的多样化群体，b）筛选功能，免疫原性和/或稳定性在步骤a）中表达的多肽，c）选择具有改变的免疫原性和/或增加稳定性的功能性多肽 ，例如 与目的多肽相比具有功能的体内半衰期，和d）任选地将编码步骤c）中选择的多肽的核苷酸序列经历步骤a）-c）的一个或多个重复循环。 在另一步骤中，步骤a）或c）的表达多肽可以与至少一个非多肽部分缀合。

    Method for down-regulating GDF-8 activity
    10.
    发明授权
    Method for down-regulating GDF-8 activity 失效
    降低GDF-8活性的方法

    公开(公告)号：US07056512B1

    公开(公告)日：2006-06-06

    申请号：US09620586

    申请日：2000-07-20

    申请人： Steen Klysner ,  Soren Mouritsen ,  Torben Halkier

    发明人： Steen Klysner ,  Soren Mouritsen ,  Torben Halkier

    IPC分类号： A61K39/00 ,  A61K39/38

    CPC分类号： C07K14/475 ,  A61K38/00 ,  A61K39/00 ,  A61K39/0005 ,  A61K48/00 ,  A61K2039/523 ,  A61K2039/53 ,  A61K2039/55516 ,  A61K2039/55522 ,  C07K2319/00

    摘要： Disclosed are novel methods for increasing muscle mass by means of immunization against Growth Differentiation Factor 8 (GDF-8, myostatin). Immunization is preferably effected by administration of analogues of GDF-8 which are capable of inducing antibody production against homologous GDF-8. Especially preferred as an immunogen is homologous GDF-8 which has been modified by introduction of one single or a few foreign, immunodominant and promiscuous T-cell epitopes while substantially preserving the tertiary structure of the homologous GDF-8. Also disclosed are nucleic acid vaccination against GDF-8 and vaccination using live vaccines as well as methods and means useful for the vaccination. Such methods and means include methods for identification of useful immunogenic GDF-8 analogues, methods for the preparation of analogues and pharmaceutical formulations, as well as nucleic acid fragments, vectors, transformed cells, polypeptides and pharmaceutical formulations.

    摘要翻译： 公开了通过免疫接种生长分化因子8（GDF-8，肌生成抑制素）来增加肌肉质量的新方法。 免疫优选通过施用能够诱导针对同源GDF-8的抗体产生的GDF-8的类似物来实现。 特别优选的是作为免疫原的同源GDF-8，其通过引入一个或多个外来的免疫显性和混杂的T细胞表位而被修饰，同时基本上保留了同源的GDF-8的三级结构。 还公开了针对GDF-8的核酸疫苗接种和使用活疫苗的疫苗接种以及用于疫苗接种的方法和装置。 这些方法和手段包括鉴定有用的免疫原性GDF-8类似物的方法，制备类似物和药物制剂的方法，以及核酸片段，载体，转化细胞，多肽和药物制剂。