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1.发明申请Apparatus for processing a sample in a liquid droplet and method of using the same 有权用于处理液滴中的样品的装置及其使用方法公开(公告)号：US20100285573A1
公开(公告)日：2010-11-11
申请号：US11984197
申请日：2007-11-14
申请人： Kwong Joo Leck , Nam Yong Kim , Jackie Y. Ying , Li Li , Yong Yeow Lee
发明人： Kwong Joo Leck , Nam Yong Kim , Jackie Y. Ying , Li Li , Yong Yeow Lee
IPC分类号： C12M1/34
CPC分类号： G01N1/31 , B01L3/5088 , B01L2300/0609 , B01L2300/0829 , B01L2300/089 , B01L2300/161 , B01L2400/0415 , C12M25/01 , G01N33/54373 , G01N2035/1037
摘要： The invention provides an apparatus and a method of processing a biological and/or chemical sample in a liquid droplet. The apparatus comprises a processing compartment, which is defined by a reservoir and an immobilisation member. The processing compartment is further adapted to accommodate a medium, which is immiscible with the liquid droplet, and of a lower surface energy than the liquid of the liquid droplet. The reservoir is defined by a circumferential wall and a base. The immobilisation member is arranged within the reservoir and comprises a surface that is patterned in such a way that it comprises at least one predefined immobilisation area. The predefined immobilisation area within the patterned surface is of a higher surface energy than the medium. Furthermore the at least one predefined area is of a higher surface energy than the remaining surface and of a sufficient width in the plane of the surface to allow, in said hydrophobic medium, the immobilisation of the liquid droplet on the hydrophilic area via hydrophilic-hydrophilic or hydrophobic-hydrophobic interactions. The remaining surface is of at most about the same surface energy as the medium. In the method of the invention the medium is disposed into the apparatus, such that the predefined immobilisation area is entirely covered by the medium. The liquid droplet is disposed onto the predefined immobilisation area, whereby the liquid droplet is immobilised thereon via hydrophilic-hydrophilic or hydrophobic-hydrophobic interactions. A process is performed on the biological and/or chemical sample in said liquid droplet.
摘要翻译： 本发明提供了一种在液滴中处理生物和/或化学样品的装置和方法。 该装置包括由储存器和固定构件限定的处理室。 处理室还适于容纳与液滴不混溶的介质和比液滴的液体低的表面能。 储存器由圆周壁和底座限定。 固定构件布置在储存器内并且包括以使得其包括至少一个预定义的固定区域的方式图案化的表面。 图案化表面内的预定义的固定区域具有比介质更高的表面能。 此外，至少一个预定义的区域具有比剩余表面更高的表面能并且在表面的平面中具有足够的宽度，以允许在所述疏水介质中通过亲水亲水性将液滴固定在亲水区域上 或疏水 - 疏水相互作用。 剩余的表面与介质的表面能最多相同。 在本发明的方法中，将介质放置在设备中，使得预定义的固定区域被介质完全覆盖。 将液滴设置在预定义的固定区域上，由此通过亲水亲水或疏水 - 疏水相互作用将液滴固定在其上。 对所述液滴中的生物和/或化学样品进行处理。
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2.发明授权Apparatus for processing a sample in a liquid droplet and method of using the same 有权用于处理液滴中的样品的装置及其使用方法公开(公告)号：US08691147B2
公开(公告)日：2014-04-08
申请号：US11984197
申请日：2007-11-14
申请人： Kwong Joo Leck , Nam Yong Kim , Jackie Y. Ying , Li Li , Yong Yeow Lee
发明人： Kwong Joo Leck , Nam Yong Kim , Jackie Y. Ying , Li Li , Yong Yeow Lee
CPC分类号： G01N1/31 , B01L3/5088 , B01L2300/0609 , B01L2300/0829 , B01L2300/089 , B01L2300/161 , B01L2400/0415 , C12M25/01 , G01N33/54373 , G01N2035/1037
摘要： The invention provides an apparatus and a method of processing a biological and/or chemical sample in a liquid droplet. The apparatus comprises a processing compartment, which is defined by a reservoir and an immobilization member. The processing compartment is further adapted to accommodate a medium, which is immiscible with the liquid droplet, and of a lower surface energy than the liquid of the liquid droplet. The reservoir is defined by a circumferential wall and a base. The immobilization member is arranged within the reservoir and comprises a surface that is patterned in such a way that it comprises at least one predefined immobilization area. The predefined immobilization area within the patterned surface is of a higher surface energy than the medium. The liquid droplet is disposed onto the predefined immobilization area, whereby the liquid droplet is immobilized thereon via hydrophilic-hydrophilic or hydrophobic-hydrophobic interactions.
摘要翻译： 本发明提供了一种在液滴中处理生物和/或化学样品的装置和方法。 该装置包括由储存器和固定构件限定的处理室。 处理室还适于容纳与液滴不混溶的介质和比液滴的液体低的表面能。 储存器由圆周壁和底座限定。 固定构件布置在储存器内并且包括以使得其包括至少一个预定义的固定区域的方式图案化的表面。 图案化表面内的预定义的固定区域具有比介质更高的表面能。 将液滴设置在预定义的固定区域上，由此通过亲水亲水或疏水 - 疏水相互作用将液滴固定在其上。
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公开(公告)号：US11260386B2
公开(公告)日：2022-03-01
申请号：US14732423
申请日：2015-06-05
申请人： Yongmei Li , Li Li
发明人： Yongmei Li , Li Li
摘要： The present disclosure relates to, inter alia, an easy-to-operate, fully closed component, which can be part of an instrument, for purification of biomolecules from biological samples, and subsequent transfer, and testing of the biomolecules, as well as an instrument comprising the component, and a method for using the component.
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公开(公告)号：US20190388590A1
公开(公告)日：2019-12-26
申请号：US16300121
申请日：2017-05-08
申请人： Stephanie Elizabeth BARRETT , Marian E. GINDY , Li LI , Ryan S. TELLER , Seth P. FORSTER , Merck Sharp & Dohme Corp.
IPC分类号： A61L27/54 , A61K9/00 , A61K31/7076 , A61K47/32 , A61K47/34
摘要： This invention relates to novel implant drug delivery systems for long-acting delivery of antiviral drugs. These compositions are useful for the treatment or prevention of human immunodeficiency virus (HIV) infection.
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公开(公告)号：US09878328B2
公开(公告)日：2018-01-30
申请号：US13811638
申请日：2011-07-25
申请人： Namyong Kim , Siah Chong Cheong , Melvin Han Wen Lye , Mark Siew Peng Phong , Li Li , Teow Soon Seah
发明人： Namyong Kim , Siah Chong Cheong , Melvin Han Wen Lye , Mark Siew Peng Phong , Li Li , Teow Soon Seah
CPC分类号： B01L3/5085 , B01L3/50853 , B01L3/50855 , B01L2200/0689 , B01L2300/0618 , B01L2300/0829 , C12M23/12 , C12M23/34
摘要： This describes an apparatus comprising a plate with a number of elements having a hydrophilic surface arranged in an array with an overlay having a hydrophobic surface. A height of the overlay is between about 5% and 100% of the diameter of the elements. The application also discloses a wall circumferential to the plate; and a removable grid insertable into the apparatus, said grid comprising dividers enclosing a number of through-holes, said through-holes spaced in the grid to allow alignment of the through-holes of the grid over the elements in the plate when said grid is inserted into the apparatus, said dividers of the grid inserted into the apparatus form sides of wells bottomed by the plate and at least one element on said plate.
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公开(公告)号：US09749435B2
公开(公告)日：2017-08-29
申请号：US13355441
申请日：2012-01-20
申请人： Li Li
发明人： Li Li
摘要： A method and apparatus to proxy notification service connections between a mobile client and a notification server. In one embodiment of the invention, a proxy receives a proxy setup request for the notification service from the mobile client. The proxy further establishes a notification connection with the notification server for the mobile client and maintains the notification connection without waking an application processor of the mobile client. The proxy receives a notification from the notification service and forwards the notification to the mobile client.
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公开(公告)号：US20170114631A1
公开(公告)日：2017-04-27
申请号：US14924611
申请日：2015-10-27
CPC分类号： E21B47/122 , E21B17/003 , E21B17/1021 , E21B17/1078 , E21B33/14 , E21B47/0005 , G01V11/002
摘要： A downhole system includes a tubular having a longitudinal axis, and a conductive trace applied on the tubular. The conductive trace is configured to deliver a signal between uphole and downhole locations of the tubular.
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公开(公告)号：US09603218B1
公开(公告)日：2017-03-21
申请号：US14657966
申请日：2015-03-13
申请人： Christopher Lee Bohler , Li Li
发明人： Christopher Lee Bohler , Li Li
IPC分类号： H05B33/08
CPC分类号： H05B33/0866 , H05B33/0851 , H05B33/0896
摘要： A method of iteratively adjusting a color of light emitted by a light source includes setting a weighted target color to an initial color of a light emitted by a light source. The method further includes determining a target color of the light by a color controller to iteratively adjust a color of the light to the target color of the light. The method also includes determining an updated weighted target color as a combination of a portion of the weighted target color and a portion of the target color. The method further includes adjusting the color of the light by the color controller to the weighted target color.
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公开(公告)号：US09535075B2
公开(公告)日：2017-01-03
申请号：US13635667
申请日：2011-03-25
申请人： Minnie M. Sarwal , Tara Sigdel , Amit Kaushal , Li Li , Wenzhong Xiao , Atul J. Butte , Purvesh Khatri
发明人： Minnie M. Sarwal , Tara Sigdel , Amit Kaushal , Li Li , Wenzhong Xiao , Atul J. Butte , Purvesh Khatri
CPC分类号： G01N33/6893 , C12Q1/6809 , C12Q1/6883 , C12Q2600/158 , G01N2800/245
摘要： Aspects of the present invention include methods for determining a transplant category of a subject having a transplant. Common mechanisms of rejection injury are uncovered across different tissue transplants, and provide a means to understand rational drug design. Various sources of tissues are examined form the patient for understanding AR mechanism (graft biopsy), as well as monitoring by minimal invasive means (blood) or non-invasive means (urine for the kidney allograft). For biomarker discovery different categories of markers are examined such as genes, proteins, peptides and antibodies. These biomarkers can help determine the subject's transplant category (e.g., acute allograft rejection (AR), stable allograft (STA), BK viremia, BK nephritis, drug toxicity or chronic allograft injury (CAI), and the like). Also provided are compositions, systems, kits and computer program products that find use in practicing the subject methods. The methods and compositions find use in a variety of applications.
摘要翻译： 本发明的方面包括用于确定具有移植物的受试者的移植类别的方法。 排除损伤的常见机制在不同的组织移植中被发现，并且提供了解理性药物设计的手段。 从患者身上检查各种来源的组织，以了解AR机制（移植物活检），以及通过最小侵入性手段（血液）或非侵入性手段（尿同种异体移植物的尿液）进行监测。 对于生物标志物发现，检查不同类别的标记物，例如基因，蛋白质，肽和抗体。 这些生物标志物可以帮助确定受试者的移植类别（例如急性同种异体移植排斥（AR），稳定的同种异体移植物（STA），BK病毒血症，BK肾炎，药物毒性或慢性同种异体移植物损伤（CAI）等）。 还提供了用于实践主题方法的组合物，系统，试剂盒和计算机程序产品。 这些方法和组合物可用于各种应用。
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