公开(公告)号：US08741680B2

公开(公告)日：2014-06-03

申请号：US13849343

申请日：2013-03-22

Applicant: Life Technologies Corporation

Inventor： Keith G. Fife ,  Kim L. Johnson ,  Mark James Milgrew

IPC: H01L21/00

CPC classification number: G01N27/4148 ,  C12Q1/6869 ,  G01J1/46 ,  G01N27/4143 ,  G01N27/4145 ,  G01N33/00 ,  G01R29/26 ,  H01L27/14609 ,  H01L27/14632 ,  H01L27/14643 ,  H01L29/66 ,  H01L2924/0002 ,  H04N5/374 ,  Y10T436/25875 ,  H01L2924/00

Abstract: A two-transistor (2T) pixel comprises a chemically-sensitive transistor (ChemFET) and a selection device which is a non-chemically sensitive transistor. A plurality of the 2T pixels may form an array, having a number of rows and a number of columns. The ChemFET can be configured in a source follower or common source readout mode. Both the ChemFET and the non-chemically sensitive transistor can be NMOS or PMOS device.

Abstract translation: 双晶体管（2T）像素包括化学敏感晶体管（ChemFET）和作为非化学敏感晶体管的选择器件。 多个2T像素可以形成具有多行和多列的阵列。 ChemFET可以配置为源跟踪器或公共源读出模式。 ChemFET和非化学敏感晶体管都可以是NMOS或PMOS器件。

公开(公告)号：US12038406B2

公开(公告)日：2024-07-16

申请号：US17064948

申请日：2020-10-07

Applicant: Life Technologies Corporation

Inventor： Kim L. Johnson ,  Jeremy Jordan ,  Peter M Levine ,  Mark James Milgrew

IPC: G01N27/414 ,  C12Q1/6869 ,  G01N33/00 ,  G01R29/26 ,  H01L27/146 ,  H01L29/66 ,  H04N25/76 ,  G01J1/46

CPC classification number: G01N27/4148 ,  C12Q1/6869 ,  G01N27/4143 ,  G01N27/4145 ,  G01N33/00 ,  G01R29/26 ,  H01L27/14609 ,  H01L27/14632 ,  H01L29/66 ,  H04N25/76 ,  G01J1/46 ,  H01L27/14643 ,  H01L2924/0002 ,  Y10T436/25875 ,  H01L2924/0002 ,  H01L2924/00

Abstract: The described embodiments may provide a chemical detection circuit that may comprise a plurality of first output circuits at a first side and a plurality of second output circuits at a second side of the chemical detection circuit. The chemical detection circuit may further comprise a plurality of tiles of pixels each placed between respective pairs of first and second output circuits. Each tile may include four quadrants of pixels. Each quadrant may have columns with designated first columns interleaved with second columns. Each first column may be coupled to a respective first output circuit in first and second quadrants, and to a respective second output circuit in third and fourth quadrants. Each second column may be coupled to a respective second output circuit in first and second quadrants, and to a respective first output circuit in third and fourth quadrants.

公开(公告)号：US11137369B2

公开(公告)日：2021-10-05

申请号：US15971857

申请日：2018-05-04

Applicant: Life Technologies Corporation

Inventor： Jonathan M. Rothberg ,  Mark James Milgrew ,  Jonathan Schultz ,  David Marran ,  Todd Rearick ,  Kim L. Johnson ,  James Bustillo

IPC: G01N27/414 ,  C12Q1/6874 ,  H01L21/82 ,  C12Q1/6869

Abstract: An apparatus comprising a chemical field effect transistor array in a circuit-supporting substrate is disclosed. The transistor array has disposed on its surface an array of sample-retaining regions capable of retaining a chemical or biological sample from a sample fluid. The transistor array has a pitch of 10 μm or less and a sample-retaining region is positioned on at least one chemical field effect transistor which is configured to generate at least one output signal related to a characteristic of a chemical or biological sample in such sample-retaining region.

公开(公告)号：US20160168634A1

公开(公告)日：2016-06-16

申请号：US15043296

申请日：2016-02-12

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Todd REARICK ,  Mark James Milgrew ,  Jonathan Schultz ,  Chris Papalias ,  Kim L. Johnson

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  G06F19/22

Abstract: In one implementation, a method is described. The method includes determining an operational characteristic of sensors of a sensor array. The method further includes selecting a group of sensors in the array based on the operational characteristic of sensors in the group. The method further includes enabling readout of the sensors in the selected group. The method further includes receiving output signals from the enabled sensors, the output signals indicating chemical reactions occurring proximate to the sensors of the sensor array.

Abstract translation: 在一个实现中，描述了一种方法。 该方法包括确定传感器阵列的传感器的操作特性。 该方法还包括基于组中的传感器的操作特性来选择阵列中的一组传感器。 该方法还包括使得能够读出所选择的组中的传感器。 所述方法还包括接收来自所述使能的传感器的输出信号，所述输出信号指示在所述传感器阵列的传感器附近发生的化学反应。

公开(公告)号：US11692964B2

公开(公告)日：2023-07-04

申请号：US16924055

申请日：2020-07-08

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Mark James Milgrew ,  Jonathan M. Rothberg ,  James Bustillo

IPC: G01N27/414 ,  G01N27/27 ,  C12Q1/6869 ,  C12Q1/6874

CPC classification number: G01N27/4145 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N27/27 ,  G01N27/414 ,  Y10T29/49002

Abstract: Methods and apparatus relating to FET arrays for monitoring chemical and/or biological reactions such as nucleic acid sequencing-by-synthesis reactions. Some methods provided herein relate to improving signal (and also signal to noise ratio) from released hydrogen ions during nucleic acid sequencing reactions.

公开(公告)号：US10100357B2

公开(公告)日：2018-10-16

申请号：US15043296

申请日：2016-02-12

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Todd Rearick ,  Mark James Milgrew ,  Jonathan Schultz ,  Chris Papalias ,  Kim L. Johnson

IPC: C12Q1/68 ,  G06F19/22 ,  C12Q1/6874

Abstract: In one implementation, a method is described. The method includes determining an operational characteristic of sensors of a sensor array. The method further includes selecting a group of sensors in the array based on the operational characteristic of sensors in the group. The method further includes enabling readout of the sensors in the selected group. The method further includes receiving output signals from the enabled sensors, the output signals indicating chemical reactions occurring proximate to the sensors of the sensor array.

公开(公告)号：US09964515B2

公开(公告)日：2018-05-08

申请号：US14862930

申请日：2015-09-23

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jonathan M. Rothberg ,  James Bustillo ,  Mark James Milgrew ,  Jonathan Schultz ,  David Marran ,  Todd Rearick ,  Kim L. Johnson

IPC: G01N27/403 ,  G01N27/414 ,  C12Q1/68 ,  H01L21/82

CPC classification number: G01N27/4145 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N27/4148 ,  H01L21/82 ,  C12Q2565/607

Abstract: An apparatus comprising a chemical field effect transistor array in a circuit-supporting substrate is disclosed. The transistor array has disposed on its surface an array of sample-retaining regions capable of retaining a chemical or biological sample from a sample fluid. The transistor array has a pitch of 10 μm or less and a sample-retaining region is positioned on at least one chemical field effect transistor which is configured to generate at least one output signal related to a characteristic of a chemical or biological sample in such sample-retaining region.

公开(公告)号：US11874250B2

公开(公告)日：2024-01-16

申请号：US17823696

申请日：2022-08-31

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jonathan M. Rothberg ,  Wolfgang Hinz ,  John F. Davidson ,  Antoine M. van Oijen ,  John Leamon ,  Martin Huber ,  Mark James Milgrew ,  James Bustillo

IPC: G01N27/414 ,  C12Q1/6874 ,  H01L21/82 ,  C12Q1/6869

CPC classification number: G01N27/4145 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N27/4148 ,  H01L21/82 ,  C12Q1/6874 ,  C12Q2565/607

Abstract: Methods and apparatus relating to FET arrays for monitoring chemical and/or biological reactions such as nucleic acid sequencing-by-synthesis reactions. Some methods provided herein relate to improving signal (and also signal to noise ratio) from released hydrogen ions during nucleic acid sequencing reactions.

公开(公告)号：US11448613B2

公开(公告)日：2022-09-20

申请号：US16101337

申请日：2018-08-10

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jonathan M. Rothberg ,  Wolfgang Hinz ,  John F. Davidson ,  Antoine M. van Oijen ,  John Leamon ,  Martin Huber ,  Mark James Milgrew ,  James Bustillo

IPC: G01N27/414 ,  C12Q1/6874 ,  H01L21/82 ,  C12Q1/6869

Abstract: Methods and apparatus relating to FET arrays for monitoring chemical and/or biological reactions such as nucleic acid sequencing-by-synthesis reactions. Some methods provided herein relate to improving signal (and also signal to noise ratio) from released hydrogen ions during nucleic acid sequencing reactions.

公开(公告)号：US20160033448A1

公开(公告)日：2016-02-04

申请号：US14794507

申请日：2015-07-08

Applicant: Life Technologies Corporation

Inventor： Mark James Milgrew

IPC: G01N27/414

CPC classification number: G01N27/4145 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N27/27 ,  G01N27/414 ,  Y10T29/49002

Abstract: Methods and apparatus relating to FET arrays for monitoring chemical and/or biological reactions such as nucleic acid sequencing-by-synthesis reactions. Some methods provided herein relate to improving signal (and also signal to noise ratio) from released hydrogen ions during nucleic acid sequencing reactions.