公开(公告)号：US20240390901A1

公开(公告)日：2024-11-28

申请号：US18792286

申请日：2024-08-01

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jon Hoshizaki ,  Mark Reed ,  David Marran ,  Patrick Leung

IPC: B01L3/00 ,  A61J1/20

Abstract: A method of applying a fluid composition to a flow cell of a sensor device includes inserting the sensor device into a holder; pipetting an aliquot of the fluid composition into a pipette tip; and applying the aliquot of the fluid composition to an opening of a fluidic connector engaged with the flow cell of the sensor device. The fluidic connector includes a body; and a plurality of fluidics interfaces formed in the body, each fluidic interface of the plurality of fluidics interfaces includes an opening, a first port in fluid communication with the opening, a second port, and a third port in fluidic communication with the second port.

公开(公告)号：US11951474B2

公开(公告)日：2024-04-09

申请号：US17304452

申请日：2021-06-21

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jonathan Schultz ,  David Marran

IPC: C12Q1/6869 ,  B01L3/00 ,  G01N27/447 ,  G01N35/10

CPC classification number: B01L3/502715 ,  B01L3/502 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/502746 ,  B01L3/502776 ,  B01L3/52 ,  C12Q1/6869 ,  G01N27/447 ,  B01J2219/00389 ,  B01J2219/00394 ,  B01L3/502769 ,  B01L2200/16 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0874 ,  B01L2300/0877 ,  B01L2400/0487 ,  G01N35/1002 ,  G01N35/1097 ,  Y10T137/0318 ,  Y10T137/0424 ,  Y10T137/85938

Abstract: The invention provides a passive fluidics circuit for directing different fluids to a common volume, such as a reaction chamber or flow cell, without intermixing or cross contamination. The direction and rate of flow through junctions, nodes and passages of the fluidics circuit are controlled by the states of upstream valves (e.g. opened or closed), differential fluid pressures at circuit inlets or upstream reservoirs, flow path resistances, and the like. Free diffusion or leakage of fluids from unselected inlets into the common outlet or other inlets at junctions or nodes is prevented by the flow of the selected inlet fluid, a portion of which sweeps by the inlets of unselected fluids and exits the fluidics circuit by waste ports, thereby creating a barrier against undesired intermixing with the outlet flow through leakage or diffusion.

公开(公告)号：US11117129B2

公开(公告)日：2021-09-14

申请号：US16543421

申请日：2019-08-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Mark Reed ,  Zheng (Derek) Xing ,  David Marran ,  Earl Zergiebel

IPC: B01L3/00 ,  C12Q1/6874

Abstract: In an example, a method for preparing a nucleotide solution includes flowing an aqueous solution from an initial solution storage of a sequencing instrument continuously through a container fluidically coupled to the sequencing instrument, the container comprising a nucleotide concentrate; and collecting the aqueous solution with nucleotide in a storage container.

公开(公告)号：US20200055046A1

公开(公告)日：2020-02-20

申请号：US16543421

申请日：2019-08-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Mark REED ,  Zheng (Derek) Xing ,  David Marran ,  Earl Zergiebel

IPC: B01L3/00 ,  C12Q1/6874

Abstract: In an example, a method for preparing a nucleotide solution includes flowing an aqueous solution from an initial solution storage of a sequencing instrument continuously through a container fluidically coupled to the sequencing instrument, the container comprising a nucleotide concentrate; and collecting the aqueous solution with nucleotide in a storage container.

公开(公告)号：US10478816B2

公开(公告)日：2019-11-19

申请号：US15348907

申请日：2016-11-10

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jonathan Schultz ,  David Marran

IPC: C12M1/00 ,  C12M3/00 ,  C12M1/34 ,  B01L3/00 ,  C12Q1/6869 ,  G01N27/447 ,  G01N35/10

Abstract: The invention provides a passive fluidics circuit for directing different fluids to a common volume, such as a reaction chamber or flow cell, without intermixing or cross contamination. The direction and rate of flow through junctions, nodes and passages of the fluidics circuit are controlled by the states of upstream valves (e.g. opened or closed), differential fluid pressures at circuit inlets or upstream reservoirs, flow path resistances, and the like. Free diffusion or leakage of fluids from unselected inlets into the common outlet or other inlets at junctions or nodes is prevented by the flow of the selected inlet fluid, a portion of which sweeps by the inlets of unselected fluids and exits the fluidics circuit by waste ports, thereby creating a barrier against undesired intermixing with the outlet flow through leakage or diffusion. The invention is particularly advantageous in apparatus for performing sensitive multistep reactions, such as pH-based DNA sequencing reactions.

公开(公告)号：US12076722B2

公开(公告)日：2024-09-03

申请号：US18107887

申请日：2023-02-09

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jon Hoshizaki ,  Patrick Leung ,  David Marran ,  Mark Reed

IPC: B01L3/00 ,  A61J1/20 ,  B01F23/00 ,  B01F23/41 ,  B01F101/23 ,  B23Q17/24 ,  C12M1/34 ,  C12Q1/04 ,  C12Q1/18 ,  C12Q1/686 ,  G01N21/3577 ,  G01N21/359 ,  G01N21/39 ,  G01N21/45 ,  G01N21/64 ,  G01N30/12 ,  G01N30/68 ,  G01N30/70 ,  G01N30/72 ,  G01N30/88 ,  G01N33/00 ,  G01N33/18 ,  G01N33/50 ,  G01N33/68

CPC classification number: B01L3/502738 ,  A61J1/2058 ,  B01L3/502715 ,  B01L3/50273 ,  B01L2300/0816

Abstract: A fluidic coupler to engage a plurality of flow cells of a sensor device includes a body and a plurality of fluidics interfaces formed in the body. Each fluidic interface of the plurality of fluidics interfaces includes an opening, a first port in fluid communication with the opening, a second port, and a third port in fluidic communication with the second port.

公开(公告)号：US11040344B2

公开(公告)日：2021-06-22

申请号：US16687672

申请日：2019-11-18

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jonathan Schultz ,  David Marran

IPC: B01L3/00 ,  C12Q1/6869 ,  G01N27/447 ,  G01N35/10

Abstract: The invention provides a passive fluidics circuit for directing different fluids to a common volume, such as a reaction chamber or flow cell, without intermixing or cross contamination. The direction and rate of flow through junctions, nodes and passages of the fluidics circuit are controlled by the states of upstream valves (e.g. opened or closed), differential fluid pressures at circuit inlets or upstream reservoirs, flow path resistances, and the like. Free diffusion or leakage of fluids from unselected inlets into the common outlet or other inlets at junctions or nodes is prevented by the flow of the selected inlet fluid, a portion of which sweeps by the inlets of unselected fluids and exits the fluidics circuit by waste ports, thereby creating a barrier against undesired intermixing with the outlet flow through leakage or diffusion.

公开(公告)号：USD914909S1

公开(公告)日：2021-03-30

申请号：US29660063

申请日：2018-08-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Designer： David Marran ,  Jon A. Hoshizaki ,  Earl Zergiebel ,  Mark T. Reed

公开(公告)号：US09964515B2

公开(公告)日：2018-05-08

申请号：US14862930

申请日：2015-09-23

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jonathan M. Rothberg ,  James Bustillo ,  Mark James Milgrew ,  Jonathan Schultz ,  David Marran ,  Todd Rearick ,  Kim L. Johnson

IPC: G01N27/403 ,  G01N27/414 ,  C12Q1/68 ,  H01L21/82

CPC classification number: G01N27/4145 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N27/4148 ,  H01L21/82 ,  C12Q2565/607

Abstract: An apparatus comprising a chemical field effect transistor array in a circuit-supporting substrate is disclosed. The transistor array has disposed on its surface an array of sample-retaining regions capable of retaining a chemical or biological sample from a sample fluid. The transistor array has a pitch of 10 μm or less and a sample-retaining region is positioned on at least one chemical field effect transistor which is configured to generate at least one output signal related to a characteristic of a chemical or biological sample in such sample-retaining region.

公开(公告)号：US20240182965A1

公开(公告)日：2024-06-06

申请号：US18437474

申请日：2024-02-09

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Mark Reed ,  Chiu Tai Andrew Wong ,  David Marran

IPC: C12Q1/6874

CPC classification number: C12Q1/6874

Abstract: A sequencing system includes an automated sequencing instrument adapted to determine variant calls for one or more extracted polynucleotide samples with a performance of at least 98.5% raw read accuracy and a run time in a range of 5 hours to 14 hours to determine variant calls for 4 extracted polynucleotide samples using a targeted assay with one DNA pool per sample and an average amplicon size in a range of 100 to 120 bases.