公开(公告)号：US09732112B2

公开(公告)日：2017-08-15

申请号：US14952149

申请日：2015-11-25

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Zhaochun Ma ,  Khairuzzaman Bashar Mullah ,  Robert G. Eason

IPC: C07H19/06 ,  C07H19/16 ,  C07D473/34 ,  C07D405/04 ,  C07D473/18 ,  C07D239/54 ,  C07H21/00 ,  C07H21/02 ,  C07H21/04 ,  C12Q1/68

CPC classification number: C07H19/16 ,  C07D239/54 ,  C07D405/04 ,  C07D473/18 ,  C07D473/34 ,  C07H19/06 ,  C07H21/00 ,  C07H21/02 ,  C07H21/04 ,  C12Q1/6806

Abstract: Methods for preparation of 2′,3′-dideoxynucleotides support structures, such as 2′,3′-dideoxyguanosine, 2′,3′-dideoxyadenosine, and 3′-deoxythymidine support structures are disclosed. Various methods of using such structures are also provided, such as their use for automated DNA synthesis and pyrophosphorolysis activated polymerization.

公开(公告)号：US20150337299A1

公开(公告)日：2015-11-26

申请号：US14816857

申请日：2015-08-03

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Vladimir I. BASHKIROV ,  Umberto Ulmanella ,  Robert G. Eason ,  Bradford J. Taft

IPC: C12N15/10 ,  C07H21/00 ,  C07H1/06

CPC classification number: C12Q1/6806 ,  C07H1/06 ,  C07H21/00 ,  C12N15/1093 ,  C12Q2523/301

Abstract: Methods and kits for preparing nucleic acid fragments from a sample of purified nucleic acid are provided. Alternatively, chromatin or other long polymers can be sheared with similar methods and kits.

公开(公告)号：US10329598B2

公开(公告)日：2019-06-25

申请号：US14816920

申请日：2015-08-03

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Vladimir I. Bashkirov ,  Umberto Ulmanella ,  Robert G. Eason ,  Bradford J. Taft

IPC: C07H21/02 ,  C12Q1/6806 ,  C07H1/06 ,  C07H21/00 ,  C12N15/10

Abstract: Methods and kits for preparing nucleic acid fragments from a sample of purified nucleic acid are provided. Alternatively, chromatin or other long polymers can be sheared with similar methods and kits.

公开(公告)号：US10246736B2

公开(公告)日：2019-04-02

申请号：US14816857

申请日：2015-08-03

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Vladimir I. Bashkirov ,  Umberto Ulmanella ,  Robert G. Eason ,  Bradford J. Taft

IPC: C12Q1/68 ,  C12Q1/6806 ,  C07H1/06 ,  C07H21/00 ,  C12N15/10

Abstract: Methods and kits for preparing nucleic acid fragments from a sample of purified nucleic acid are provided. Alternatively, chromatin or other long polymers can be sheared with similar methods and kits.

公开(公告)号：US20170305954A1

公开(公告)日：2017-10-26

申请号：US15643053

申请日：2017-07-06

Applicant: Life Technologies Corporation

Inventor： Zhaochun Ma ,  Khairuzzaman Bashar Mullah ,  Robert G. Eason

IPC: C07H19/16 ,  C07H21/04 ,  C07H21/02 ,  C07H19/06 ,  C07D239/54 ,  C07D473/34 ,  C07D473/18 ,  C07D405/04 ,  C12Q1/68 ,  C07H21/00

CPC classification number: C07H19/16 ,  C07D239/54 ,  C07D405/04 ,  C07D473/18 ,  C07D473/34 ,  C07H19/06 ,  C07H21/00 ,  C07H21/02 ,  C07H21/04 ,  C12Q1/6806

Abstract: Methods for preparation of 2′,3′-dideoxynucleotides support structures, such as 2′,3′-dideoxyguanosine, 2′,3′-dideoxyadenosine, and 3′-deoxythymidine support structures are disclosed. Various methods of using such structures are also provided, such as their use for automated DNA synthesis and pyrophosphorolysis activated polymerization.