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    Substituted nucleic acid mimics
    1.
    发明授权
    Substituted nucleic acid mimics 失效
    取代的核酸模拟物

    公开(公告)号：US07825215B1

    公开(公告)日：2010-11-02

    申请号：US08612661

    申请日：1996-03-08

    申请人： Leif Christensen ,  Henrik Frydenlund Hansen ,  Peter E. Nielsen

    发明人： Leif Christensen ,  Henrik Frydenlund Hansen ,  Peter E. Nielsen

    IPC分类号： C07K2/00 ,  A61K38/00 ,  C07K1/00 ,  C12Q1/68 ,  G01N33/566 ,  C07H21/02

    CPC分类号： C07H21/00

    摘要： Compositions and methods are provided for the nucleic acid mimic determination of nucleic acids. The compositions and methods may be used in the diagnosis and treatment of diseases amenable through modulation of nucleic acids which encode proteins that are implicated in disease states. In accordance with preferred embodiments, mimics are comprised of non-naturally occurring backbones to which are appended modified heterocyclic bases. Such bases preferably have sterically bulky substituents 1, 2, or 3 atoms removed from the sites of attachment to the backbone.

    摘要翻译： 提供了用于核酸的核酸模拟测定的组合物和方法。 组合物和方法可以用于诊断和治疗通过调节编码与疾病状态有关的蛋白质的核酸而适应的疾病。 根据优选实施方案，模拟物由非天然存在的骨架组成，其中是附加的修饰的杂环碱基。 这些碱优选具有从连接于主链的位点除去的空间庞大的取代基1,2或3个原子。

    Admixture comprising a target molecule and a nucleic acid mimic comprising sterically bulky substituents
    2.
    发明授权
    Admixture comprising a target molecule and a nucleic acid mimic comprising sterically bulky substituents 失效
    包含靶分子和包含空间庞大取代基的核酸模拟物的外加剂

    公开(公告)号：US07569659B1

    公开(公告)日：2009-08-04

    申请号：US09142326

    申请日：1997-03-07

    申请人： Leif Christensen ,  Henrik Frydenlund Hansen ,  Peter E. Nielsen

    发明人： Leif Christensen ,  Henrik Frydenlund Hansen ,  Peter E. Nielsen

    IPC分类号： C12Q1/68 ,  C07K7/00

    CPC分类号： C07H21/00

    摘要： The invention concerns compositions comprising a nucleic acid mimic. The compositions may be used in the diagnosis and treatment of diseases amenable through modulation of nucleic acids which encode proteins that are implicated in disease states. In accordance with preferred embodiments, mimics are comprised of non-naturally occurring backbones to which are appended modified heterocyclic bases. Such bases preferably have sterically bulky substituents 1, 2, or 3 atoms removed from the sites of attachment to the backbone.

    摘要翻译： 本发明涉及包含核酸模拟物的组合物。 该组合物可用于诊断和治疗通过调节编码与疾病状态有关的蛋白质的核酸而适应的疾病。 根据优选实施方案，模拟物由非天然存在的骨架组成，其中是附加的修饰的杂环碱基。 这些碱优选具有从连接于主链的位点除去的空间庞大的取代基1,2或3个原子。

    Conjugated peptide nucleic acids having enhanced cellular uptake
    6.
    发明授权
    Conjugated peptide nucleic acids having enhanced cellular uptake 失效
    具有增强的细胞摄取的共轭肽核酸

    公开(公告)号：US06350853B1

    公开(公告)日：2002-02-26

    申请号：US09404430

    申请日：1999-09-23

    申请人： Peter E. Nielsen ,  Helle Knudsen

    发明人： Peter E. Nielsen ,  Helle Knudsen

    IPC分类号： A61K9127

    CPC分类号： C12N15/113 ,  A61K9/1272 ,  A61K38/00 ,  A61K47/62 ,  C07H21/00 ,  C07K14/003 ,  C12N2310/15 ,  C12N2310/3181 ,  C12Q1/6813 ,  C12Q2525/107 ,  C12Q2537/119

    摘要： Peptide nucleic acids conjugated to lipophilic groups and incorporated into liposomes exhibit enhanced cellular uptake and distribution. Cellular uptake and distribution of peptide nucleic acids also increases with the introduction of an amino acid side chain into the backbone of peptide nucleic acids. Methods of modulating cellular uptake and methods for treating animals are provided. The peptide nucleic acids of the invention comprise naturally-occurring nucleobases and non-naturally-occurring nucleobases attached to a polyamide backbone.

    摘要翻译： 缀合到亲脂基团并掺入脂质体的肽核酸表现出增强的细胞摄取和分布。 肽核酸的细胞摄取和分布也随着将氨基酸侧链引入肽核酸的主链而增加。 提供调节细胞摄取的方法和治疗动物的方法。 本发明的肽核酸包含天然存在的核碱基和连接到聚酰胺主链上的非天然存在的核碱基。

    Use of nucleic acid analogues in diagnostics and analytical procedures
    8.
    发明授权
    Use of nucleic acid analogues in diagnostics and analytical procedures 失效
    在诊断和分析程序中使用核酸类似物

    公开(公告)号：US06357163B1

    公开(公告)日：2002-03-19

    申请号：US08150156

    申请日：1994-05-04

    申请人： Ole Buchardt ,  Michael Egholm ,  Peter E. Nielsen ,  Rolf H. Berg

    发明人： Ole Buchardt ,  Michael Egholm ,  Peter E. Nielsen ,  Rolf H. Berg

    IPC分类号： C12Q168

    CPC分类号： C07H21/00 ,  A61K38/00 ,  C07K5/06026 ,  C07K5/06139 ,  C07K7/06 ,  C07K7/08 ,  C07K14/003 ,  C12Q1/68 ,  C12Q1/6813 ,  C12Q1/6832 ,  C12Q1/6869 ,  C12Q2535/107 ,  C12Q2525/107

    摘要： Methods of capture, recognition, detection, identification or quantitation of nucleic acids and diagnostics uses generally are described in which are used: (a) a peptide nucleic acid (PNA) comprising a polyamide backbone bearing a plurality of ligands at respective spaced locations along said backbone, said ligands being each independently naturally occurring nucleobases, non-naturally occurring nucleobases or nucleobase-binding groups, each said ligand being bound directly or indirectly to a nitrogen atom in said backbone, and said ligand bearing nitrogen atoms mainly being separated from one another in said backbone by from 4 to 8 intervening atoms; or (b) a nucleic acid analogue capable of hybridizing to a nucleic acid of complementary sequence to form a hybrid which is more stable against denaturation by heat than a hybrid between the conventional deoxyribonucleotide corresponding to said analogue and said nucleic acid; or (c) a nucleic acid analogue capable of hybridizing to a double stranded nucleic acid in which one strand has a sequence complementary to said analogue, so as to displace the other strand from said one strand.

    摘要翻译： 通常描述了核酸和诊断用途的捕获，识别，检测，鉴定或定量的方法，其中使用：（a）肽核酸（PNA），其包含聚酰胺主链，所述聚酰胺主链在所述 所述配体各自独立地是天然存在的核碱基，非天然存在的核碱基或核碱基结合基团，每个所述配体直接或间接地结合到所述主链中的氮原子，并且所述配位体氮原子主要彼此分离 在所述骨架中通过4至8个插入的原子; 或（b）能够与互补序列核酸杂交的核酸类似物，以形成与对应于所述类似物的所述类似物和所述核酸的常规脱氧核糖核苷酸之间的杂交体相比，通过加热变性更稳定的杂交体; 或（c）能够与双链核酸杂交的核酸类似物，其中一条链具有与所述类似物互补的序列，以便从所述一条链取代另一条链。