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公开(公告)号:US6069000A
公开(公告)日:2000-05-30
申请号:US136628
申请日:1998-08-19
IPC分类号: A23L2/84 , A23L21/12 , A23L29/00 , A23L29/231 , C12G1/022 , C12N9/18 , C07H21/04 , C12N1/12 , C12N1/14 , C12N1/20
CPC分类号: C12Y301/01011 , A23L2/84 , A23L21/12 , A23L29/06 , A23L29/231 , C12G1/0203 , C12N9/18
摘要: The present invention relates to an enzyme with pectin esterase activity, a DNA construct encoding the enzyme with pectin esterase activity, a method of producing the enzyme, an enzyme composition comprising said enzyme with pectin esterase activity, and the use of said enzyme and enzyme composition for a number of industrial applications.
摘要翻译: 本发明涉及具有果胶酯酶活性的酶,编码具有果胶酯酶活性的酶的DNA构建体,该酶的制备方法,含有果胶酯酶活性的酶的酶组合物,以及所述酶和酶组成的用途 为一些工业应用。
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公开(公告)号:US20070116744A1
公开(公告)日:2007-05-24
申请号:US11605148
申请日:2006-11-28
申请人: Lene Andersen , Martin Schulein , Niels Lange , Mads Bjornvad , Soren Moller , Sanne O Glad , Markus Kauppinen , Kirk Schnorr , Lars Kongsbak
发明人: Lene Andersen , Martin Schulein , Niels Lange , Mads Bjornvad , Soren Moller , Sanne O Glad , Markus Kauppinen , Kirk Schnorr , Lars Kongsbak
CPC分类号: C12N9/2465 , A23K10/14 , A23K20/189 , C07K2319/20 , C11D3/386 , C12N9/88 , D06L4/40 , D06M16/003 , D21C5/005 , Y10T442/20
摘要: The present invention relates to pectate lyases comprising the amino acid sequence Asn Leu Asn Ser Arg Val Pro (NLNSRVP) (amino acids 236-242 of SEQ ID NO: 2) belonging to Family 1 of polysaccharide lyases have good performance in industrial processes under neutral or alkaline conditions such as laundering and textile processing. The pectate lyase may be derivable from Bacillus species.
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公开(公告)号:US20050070003A1
公开(公告)日:2005-03-31
申请号:US10965499
申请日:2004-10-14
申请人: Martin Schulein , Torben Henriksen , Lene Andersen , Soren Lassen , Markus Kauppinen , Lene Lange , Ruby Nielsen , Shinobu Takagi , Michiko Ihara
发明人: Martin Schulein , Torben Henriksen , Lene Andersen , Soren Lassen , Markus Kauppinen , Lene Lange , Ruby Nielsen , Shinobu Takagi , Michiko Ihara
IPC分类号: C12N15/09 , C05F11/08 , C05F17/00 , C07G99/00 , C11D3/386 , C12N1/19 , C12N9/42 , C12N15/00 , C12R1/645 , C12R1/69 , C12R1/77 , C12R1/80 , C12S3/04 , C12S11/00 , D06M13/02 , D06M13/322 , D06M13/46 , D06M16/00 , D06P5/02 , D06P5/15 , D21C5/00 , D21H11/16 , D21H11/20 , D21H17/00 , D21H21/10
CPC分类号: C12N9/2437 , A61K38/00 , C05F11/08 , C05F17/0036 , C07K2319/00 , C11D3/386 , C12P19/14 , C12Y302/01004 , D06M16/003 , D06P5/02 , D06P5/158 , D21C5/005 , D21H17/005 , D21H21/10 , Y02P20/145 , Y02W30/43
摘要: The present invention relates to enzyme preparations consisting essentially of an enzyme which has cellulytic activity and comprises a first amino acid sequence having the following sequence (SEQ ID NO: 79) ThrArgXaaXaaAspCysCysXaaXaaXaaCysXaaTrpXaa 1234567891011121314 and a second amino acid sequence having the following sequence TrpCysCysXaaCys(SEQ ID NO: 80) 12345 wherein, at position 3 of the first sequence, the amino acid is Trp, Tyr or Phe; at position 4 of the first sequence, the amino acid is Trp, Tyr or Phe; at position 8 of the first sequence, the amino acid is Arg, Lys or His; at positions 9, 10, 12 and 14, respectively, of the first sequence, and at position 4 of the second sequence, the amino acid is any of the 20 naturally occurring amino acid residues with the provisos that, in the first amino acid sequence, (i) when the amino residue at position 12 is Ser, then the amino acid residue at position 14 is not Ser, and (ii) when the amino residue at position 12 is Gly, then the amino acid residue at position 14 is not Ala, performs very well in industrial applications such as laundry compositions, for biopolishing of newly manufactured textiles, for providing an abraded look of cellulosic fabric or garment, and for treatment of paper pulp. Further, the invention relates to DNA constructs encoding such enzymes, a method for providing a gene encoding for such enzymes, a method of producing the enzymes, enzyme preparations containing such enzymes, and the use of these enzymes for a number of industrial applications.
摘要翻译: 本发明涉及酶制剂,其基本上由具有纤维素分解活性的酶组成,并且包含具有以下序列的第一氨基酸序列和具有以下序列的第二个氨基酸序列,其中在第一序列的3位是氨基酸 是Trp,Tyr或Phe; 在第一序列的位置4处,氨基酸是Trp,Tyr或Phe; 在第一序列的位置8,氨基酸是Arg,Lys或His; 在第一序列的第9,10,12和14位,第2个序列的第4位,氨基酸是20个天然存在的氨基酸残基中的任一个,条件是在第一个氨基酸序列 ,(i)当第12位的氨基残基为Ser时,第14位的氨基酸残基不为Ser,(ii)当第12位的氨基残基为Gly时,第14位的氨基酸残基为 Ala在工业应用中表现良好,例如洗衣组合物,用于生物抛光新制造的纺织品,用于提供纤维素织物或衣服的磨损外观以及纸浆处理。 此外,本发明涉及编码这些酶的DNA构建体,提供编码这种酶的基因的方法,生产酶的方法,含有这些酶的酶制剂,以及这些酶用于许多工业应用的用途。
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公开(公告)号:US20050009068A1
公开(公告)日:2005-01-13
申请号:US10849106
申请日:2004-05-19
CPC分类号: C12Y301/01005 , C12N9/18
摘要: The inventors have isolated lysophospholipases from Aspergillus (A. niger and A. oryzae) having molecular masses of about 68 kDa and amino acid sequences of 600-604 amino acid residues. The novel lysophospholipases have only a limited homology to known amino acid sequences. The inventors also isolated genes encoding the novel enzymes and cloned them into E. coli strains.
摘要翻译: 本发明人具有分子量为约68kDa和氨基酸序列为600-604个氨基酸残基的曲霉(A.niger和米曲霉)的分离的溶血磷脂酶。 新型溶血磷脂酶与已知的氨基酸序列仅具有有限的同源性。 本发明人还分离了编码新型酶的基因并将其克隆到大肠杆菌菌株中。
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