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    SIMULTANEOUS QUANTIFICATION OF NUCLEIC ACIDS IN DISEASED CELLS
    1.
    发明申请
    SIMULTANEOUS QUANTIFICATION OF NUCLEIC ACIDS IN DISEASED CELLS 失效
    在细胞中同时检测核酸的量

    公开(公告)号：US20070196824A1

    公开(公告)日：2007-08-23

    申请号：US11686499

    申请日：2007-03-15

    申请人： Lieven Stuyver ,  Michael Otto

    发明人： Lieven Stuyver ,  Michael Otto

    IPC分类号： C12Q1/70 ,  C12Q1/68

    CPC分类号： C07H19/06 ,  C07H19/048 ,  C07H19/10 ,  C07H19/16 ,  C07H19/20 ,  C07H21/04 ,  C12Q1/6809 ,  C12Q1/6876 ,  C12Q1/689 ,  C12Q1/6895 ,  C12Q1/701 ,  C12Q2600/136 ,  C12Q2600/142 ,  C12Q2600/158 ,  Y10S435/81 ,  C12Q2561/101 ,  C12Q2545/101 ,  C12Q2537/143

    摘要： Processes and methods for the simultaneous quantification of nucleic acids in diseased cells that are based on real-time PCR are provided. The real-time PCR protocol is an excellent tool for reliable quantification of in vitro drug screening and evaluation protocols to determine the efficacy of potential anti-viral agents. Quantification using these simultaneous PCR cycle threshold (Ct) detection techniques during one-step real-time RT-PCR (Applied Biosystems, CA) eliminates the variability resulting from quantification of end-point RT-PCR products. In addition, the mitochondrial toxicity assay is an added tool to assess potential side-effects for these chemotherapeutic agents.

    摘要翻译： 提供了基于实时PCR同时定量病变细胞中核酸的方法和方法。 实时PCR方案是用于可靠定量体外药物筛选和评估方案以确定潜在的抗病毒剂的功效的优良工具。 在一步实时RT-PCR（Applied Biosystems，CA）中使用这些同时PCR循环阈值（Ct）检测技术的定量消除了由终点RT-PCR产物定量产生的变异性。 此外，线粒体毒性测定是评估这些化学治疗剂的潜在副作用的一个附加工具。

    Compounds for the treatment of flaviviridae infections
    3.
    发明申请
    Compounds for the treatment of flaviviridae infections 审中-公开
    用于治疗黄病毒科感染的化合物

    公开(公告)号：US20050049204A1

    公开(公告)日：2005-03-03

    申请号：US10812448

    申请日：2004-03-29

    申请人： Michael Otto ,  Lieven Stuyver

    发明人： Michael Otto ,  Lieven Stuyver

    IPC分类号： A61K31/425 ,  A61K31/675 ,  A61K31/7056

    CPC分类号： A61K31/70 ,  A61K31/34 ,  A61K31/38 ,  A61K31/40 ,  A61K31/41 ,  A61K31/415 ,  A61K31/42 ,  A61K31/425 ,  A61K31/505 ,  A61K31/715

    摘要： The disclosed invention is a composition for and a method of treating a Flaviviridae infections, such as bovine viral diarrhea virus (“BVDV”), Dengue Virus (DENV), West Nile Virus (WNV) and hepatitis C virus (HCV), as well as abnormal cellular proliferation, in a host, including animals, and especially humans, using a nucleoside of general formula (I)-(V) or N-(phosphonoacetyl)-L-aspartate (PALA), or a pharmaceutically acceptable salt or prodrug thereof.

    摘要翻译： 所公开的发明是用于治疗黄病毒科感染的组合物和方法，例如牛病毒性腹泻病毒（“BVDV”），登革热病毒（DENV），西尼罗河病毒（WNV）和丙型肝炎病毒（HCV）） 作为异常的细胞增殖，使用通式（I） - （V）或N-（膦酰基乙酰基）-L-天冬氨酸（PALA）的核苷或其药学上可接受的盐或前体药物，包括动物，特别是人的宿主， 其中。

    Sequences of hepatitis C virus genotypes and their use as prophylactic, therapeutic and diagnostic agents
    5.
    发明授权
    Sequences of hepatitis C virus genotypes and their use as prophylactic, therapeutic and diagnostic agents 失效
    丙型肝炎病毒基因型序列及其作为预防，治疗和诊断剂的用途

    公开(公告)号：US07129337B1

    公开(公告)日：2006-10-31

    申请号：US08836075

    申请日：1995-10-23

    申请人： Geert Maertens ,  Lieven Stuyver

    发明人： Geert Maertens ,  Lieven Stuyver

    IPC分类号： C12Q1/68 ,  C07H21/02 ,  A61K39/29

    CPC分类号： C07K14/005 ,  A61K38/00 ,  A61K39/00 ,  A61K2039/525 ,  C12N2770/24222

    摘要： The present invention relates to new genomic nucleotide sequences and amino acid sequences corresponding to the coding region of these genomes. The invention relates to new HCV types and subtypes sequences which are different from the known HCV types and subtypes sequences. More particularly, the present invention relates to new HCV type 7 sequences, new HCV type 9 sequences, new HCV type 10 and new HCV type 11 sequences. Also, the present invention relates to new HCV type 1 sequences of subtypes 1d, 1e, 1f and 1g; new HCV type 2 sequences of subtypes 2e, 2f, 2g, 2h, 2i, 2k and 2l; new HCV type 3 sequences of subtype 3g, new HCV type 4 sequences of subtypes 4k, 4l and 4m; a process for preparing them, and their use for diagnosis, prophylaxis and therapy. More particularly, the present invention provides new type-specific sequences of the Core, the E1 and the NS5 regions of new HCV types 7, 9, 10 and 11, as well as of new variants (subtypes) of HCV types 1, 2, 3 and 4. These new HCV sequences are useful to diagnose the presence of HCV type 1, and/or type 2, and/or type 3, and/or type 4, and/or type 7, and/or type 9, and/or type 10, and/or type 11 genotypes or serotypes in a biological sample. Moreover, the availability of these new type-specific sequences can increase the overall sensitivity of HCV detection and should also prove to be useful for prophylactic and therapeutic purposes.

    摘要翻译： 本发明涉及对应于这些基因组的编码区的新的基因组核苷酸序列和氨基酸序列。 本发明涉及与已知的HCV类型和亚型序列不同的新的HCV类型和亚型序列。 更具体地，本发明涉及新的HCV 7型序列，新型HCV 9型序列，新型HCV 10型和新型HCV 11型序列。 此外，本发明涉及新型的1型，1e型，1型和1型的HCV型1型序列; 亚型2e，2f，2g，2h，2i，2k和2l的新HCV 2型序列; 亚型3g的新HCV 3型序列，亚型4k，4l和4m的新型HCV 4型序列; 它们的制备过程及其在诊断，预防和治疗中的应用。 更具体地，本发明提供新型HCV 7型，9型，10型和11型的核心，E1和NS5区域的新型特异性序列，以及HCV类型1,2和2的新变体（亚型） 这些新的HCV序列可用于诊断HCV 1型和/或2型和/或3型和/或4型和/或7型和/或9型的存在，以及 /或10型，和/或11型基因型或血清型。 此外，这些新型特异性序列的可用性可以提高HCV检测的总体灵敏度，并且还应证明可用于预防和治疗目的。

    Process for typing HCV isolates
    6.
    发明授权
    Process for typing HCV isolates 失效
    分离HCV分离株的方法

    公开(公告)号：US06548244B2

    公开(公告)日：2003-04-15

    申请号：US09899044

    申请日：2001-07-06

    申请人： Geert Maertens ,  Lieven Stuyver ,  Rudi Rossau ,  Hugo Van Heuverswyn

    发明人： Geert Maertens ,  Lieven Stuyver ,  Rudi Rossau ,  Hugo Van Heuverswyn

    IPC分类号： C12Q170

    CPC分类号： C12Q1/707 ,  C12Q1/6834 ,  C12Q2563/131 ,  C12Q2545/101 ,  C12Q2525/101 ,  C12Q2531/113

    摘要： The invention relates to a process for genotyping any HCV isolate present in a biological sample, previously identified as being HCV positive, and for classifying said isolate according to the percentage of homology with other HCV isolates, comprising the steps of: contacting said sample in which the ribonucleotides or deoxyribonucleotides have been made accessible, if need be, under suitable denaturation, with at least one probe from about 10 to about 40 nucleotides, with said probe being liable to hybridize to a region being in the domain extending from nucleotide at position −291 to nucleotide at position −66 of the 5′ untranslated region of one of the HCV isolates represented by their cDNA sequences, with said numbering of position beginning with the first ATG codon of the open reading frame encoding the HCV polyprotein, or with said probe being complementary to the above-defined probes, detecting the complexes possibly formed between said probe and the nucleotide sequence of the HCV isolate to be identified.

    摘要翻译： 本发明涉及用于对先前鉴定为HCV阳性的生物样品中存在的任何HCV分离物进行基因分型的方法，以及根据与其它HCV分离株的同源性百分比对所述分离物进行分类的方法，包括以下步骤： 如果需要的话，核糖核苷酸或脱氧核糖核苷酸可以在合适的变性下与至少一个约10至约40个核苷酸的探针接触，所述探针易于与位置延伸的结构域延伸的区域杂交。 291到由其cDNA序列表示的HCV分离物之一的5'非翻译区的-66位的核苷酸，其中所述编号的位置开始于编码HCV多聚蛋白的开放阅读框的第一个ATG密码子，或与所述探针 与上述定义的探针互补，检测可能形成于所述探针和HCV核苷酸序列之间的复合物 分离鉴定。

    Process for typing of HCV isolates
    8.
    发明申请
    Process for typing of HCV isolates 失效
    HCV分离株的分型方法

    公开(公告)号：US20100120121A1

    公开(公告)日：2010-05-13

    申请号：US11826099

    申请日：2007-07-12

    申请人： Geert Maertens ,  Lieven Stuyver ,  Rudi Rossau ,  Hugo Van Heuverswyn

    发明人： Geert Maertens ,  Lieven Stuyver ,  Rudi Rossau ,  Hugo Van Heuverswyn

    IPC分类号： C12N7/01 ,  C07H21/00

    CPC分类号： C12Q1/707

    摘要： The invention relates to a process for genotyping any HCV isolate present in a biological sample, previously identified as being HCV positive, and for classifying said isolate according to the percentage of homology with other HCV isolates, comprising the steps of: contacting said sample in which the ribonucleotides or deoxyribonucleotides have been made accessible, if need be, under suitable denaturation, with at least one probe from about 10 to about 40 nucleotides, with said probe being liable to hybridize to a region being in the domain extending from nucleotide at position −291 to nucleotide at position −66 of the 5′ untranslated region of one of the HCV isolates represented by their cDNA sequences, with said numbering of position beginning with the first ATG codon of the open reading frame encoding the HCV polyprotein, or with said probe being complementary to the above-defined probes, detecting the complexes possibly formed between said probe and the nucleotide sequence of the HCV isolate to be identified.

    摘要翻译： 本发明涉及用于对先前鉴定为HCV阳性的生物样品中存在的任何HCV分离物进行基因分型的方法，以及根据与其它HCV分离株的同源性百分比对所述分离物进行分类的方法，包括以下步骤： 如果需要的话，核糖核苷酸或脱氧核糖核苷酸可以在合适的变性下与至少一个约10至约40个核苷酸的探针接触，所述探针易于与位置延伸的结构域延伸的区域杂交。 291到由其cDNA序列表示的HCV分离物之一的5'非翻译区的-66位的核苷酸，其中所述编号的位置开始于编码HCV多蛋白的开放阅读框的第一个ATG密码子，或与所述探针 与上述探针互补，检测可能在所述探针和核苷酸序列之间形成的复合物 HCV分离株被鉴定。

    Method for typing and detecting HBV
    10.
    发明申请
    Method for typing and detecting HBV 有权
    HBV的分型和检测方法

    公开(公告)号：US20050175990A1

    公开(公告)日：2005-08-11

    申请号：US10606879

    申请日：2003-06-27

    申请人： Lieven Stuyver ,  Rudi Rossau ,  Geert Maertens

    发明人： Lieven Stuyver ,  Rudi Rossau ,  Geert Maertens

    IPC分类号： C12N15/36 ,  C12Q1/70 ,  C12Q1/68

    CPC分类号： C12Q1/706

    摘要： The present invention relates to a method for detection and/or genetic analysis of HBV in a biological sample, comprising hybridizing the polynucleic acids of the sample with a combination of at least two nucleotide probes, with said combination hybridizing specifically to a mutant target sequence chosen from the HBV RT pol gene region and/or to a mutant target sequence chosen from the HBV preCore region and/or to a mutant target sequence chosen from the HBsAg region of HBV and/or to a HBV genotype-specific target sequence, with said target sequences being chosen from FIG. 1, and with said probes being applied to known locations on a solid support and with said probes being capable of hybridizing to the polynucleic acids of the sample under the same hybridization and wash conditions, or with said probes hybridizing specifically with a sequence complementary to any of said target sequences, or a sequence where T of said target sequence is replaced by U; and detecting the hybrids formed; and inferring the HBV genotype and/or mutants present in said sample from the differential hybridization signal(s) obtained. The invention further relates to sets of nucleotide probes and possibly primers useful in said methods as well as to their use in a method for typing and/or detecting HBV and to assay kits using the same.

    摘要翻译： 本发明涉及用于生物样品中HBV的检测和/或遗传分析的方法，包括将所述样品的多核酸与至少两个核苷酸探针的组合杂交，所述组合特异性与选择的突变靶序列杂交 从HBV RT pol基因区域和/或选自HBV preCore区域的突变靶序列和/或选自HBV的HBsAg区域和/或HBV基因型特异性靶序列的突变靶序列，其中所述 靶序列选自图1。 1，并且所述探针被施加到固体支持物上的已知位置，并且所述探针能够在相同的杂交和洗涤条件下与样品的多核酸杂交，或者与所述探针特异性地与任何与任何 或所述靶序列的T被U代替的序列; 并检测形成的杂种; 并从所获得的差异杂交信号中推断存在于所述样品中的HBV基因型和/或突变体。 本发明还涉及可用于所述方法的核苷酸探针和可能的引物的集合，以及它们在用于分型和/或检测HBV的方法中的用途以及使用其的测定试剂盒。