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公开(公告)号：US08815573B2
公开(公告)日：2014-08-26
申请号：US13847961
申请日：2013-03-20
发明人： Stephen F. Gorfien , Richard Fike , Glenn P. Godwin , Joyce L. Wolanske , David A. Epstein , Dale Gruber , Don McClure , Paul J. Price
CPC分类号： C12N5/0682 , C07K14/61 , C12N5/0037 , C12N5/0043 , C12N5/005 , C12N5/0603 , C12N7/00 , C12N9/2402 , C12N9/2471 , C12N15/85 , C12N2500/14 , C12N2500/16 , C12N2500/22 , C12N2500/24 , C12N2500/32 , C12N2500/34 , C12N2500/35 , C12N2500/38 , C12N2500/74 , C12N2500/76 , C12N2500/90 , C12N2500/95 , C12N2501/90 , C12N2501/91 , C12N2710/10051 , C12Y302/01023
摘要： The present invention provides a cell culture medium formulation that supports the in vitro cultivation, particularly in suspension, of mammalian cells, particularly epithelial cells and fibroblast cells, and methods for cultivating mammalian cells in suspension in vitro using these media. The present invention also provides chemically defined, protein-free eukaryotic cell culture media comprising an iron chelate and zinc, which is capable of supporting the growth (and particularly the high-density growth of mammalian cells) in suspension culture, increasing the level of expression of recombinant protein in cultured cells, and/or increasing virus production in cultured cells. The invention also provides kits for use in the cultivation of a mammalian epithelial cell that comprise one or more containers, wherein a first container contains the culture medium of the invention. These kits may further comprise one or more additional containers containing one or more supplements.
摘要翻译： 本发明提供了支持哺乳动物细胞，特别是上皮细胞和成纤维细胞的体外培养，特别是悬浮液的细胞培养基制剂，以及使用这些培养基在体外悬浮培养哺乳动物细胞的方法。 本发明还提供化学上定义的，无蛋白质的真核细胞培养基，其包含铁螯合物和锌，其能够支持悬浮培养物中的生长（特别是哺乳动物细胞的高密度生长），从而增加表达水平 的培养细胞中的重组蛋白，和/或增加培养细胞中的病毒产生。 本发明还提供用于培养包含一个或多个容器的哺乳动物上皮细胞的试剂盒，其中第一容器含有本发明的培养基。 这些试剂盒还可以包含一种或多种含有一种或多种补充剂的另外的容器。
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公开(公告)号：US10066200B2
公开(公告)日：2018-09-04
申请号：US15198237
申请日：2016-06-30
发明人： Richard Fike , Shawn Barrett , Zhou Jiang , David Judd
摘要： Compositions and methods are described for preparing media, feeds, and supplements. Such methods and medias may display increased stability of labile components and may use, for example, microsuspension and/or encapsulation technologies, chelation, and optionally, coating and/or mixing the labile compounds with anti-oxidants. The compositions may withstand thermal and/or irradiation treatment and have reduced virus number. These techniques may result in product with extended shelf-life, extended release of their internal components into culture, or in product that can be added aseptically into a bioreactor using minimal volumes. The compositions and methods may optimize the bioproduction workflow and increase efficiency.
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公开(公告)号：US09410118B2
公开(公告)日：2016-08-09
申请号：US14367413
申请日：2012-12-21
发明人： Richard Fike , Shawn Barrett , Zhou Jiang , David Judd
CPC分类号： C12N5/005 , B01J13/06 , B01J13/22 , B29C67/24 , B29L2009/00 , C12N5/0018 , C12N2500/30 , C12N2500/38 , C12N2511/00 , C12N2533/40 , C12N2533/74
摘要： Compositions and methods are described for preparing media, feeds, and supplements. Such methods and medias may display increased stability of labile components and may use, for example, microsuspension and/or encapsulation technologies, chelation, and optionally, coating and/or mixing the labile compounds with anti-oxidants. The compositions may withstand thermal and/or irradiation treatment and have reduced virus number. These techniques may result in product with extended shelf-life, extended release of their internal components into culture, or in product that can be added aseptically into a bioreactor using minimal volumes. The compositions and methods may optimize the bioproduction workflow and increase efficiency.
摘要翻译： 描述了制备培养基，饲料和补品的组合物和方法。 这样的方法和介质可以显示不稳定组分的增加的稳定性，并且可以使用例如微悬浮和/或封装技术，螯合，以及任选地，将不稳定化合物与抗氧化剂包衣和/或混合。 组合物可以承受热和/或照射治疗并且具有减少的病毒数量。 这些技术可能导致产品具有延长的保质期，将其内部组分延长释放到培养物中，或可以无菌地添加到生物反应器中使用最小体积的产品中。 组合物和方法可以优化生物生产工作流程并提高效率。
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公开(公告)号：US11028361B2
公开(公告)日：2021-06-08
申请号：US16801668
申请日：2020-02-26
发明人： Richard Fike , Bruce Branchaud , Shawn Barrett
摘要： Methods for increasing the stability of, or protecting, labile components such as ethanolamine, growth factors, vitamins, etc., in compositions such as a cell culture medium. Stability of the labile compound is increased either, by derivatization of the labile compound with chemicals, or by sequestering the labile compound. Sequestering can be done either by encapsulation within a microcapsule, or by the use of sequestering agents. Encapsulation includes the encapsulation of dendrimers complexes of susceptible compounds within the microcapsule, thereby providing the controlled release of the susceptible compound that was protected.
These methods may improve and extend storage conditions of compositions comprising the labile compounds, improve shipping and handling of compositions comprising the labile compounds, such as dry media formulations, at room temperature rather than at lower temperatures thereby decreasing shipping costs. Stabilization of labile compounds in compositions such as dry format media can be viewed as a contribution to green technology.-
公开(公告)号：US10822584B2
公开(公告)日：2020-11-03
申请号：US15566899
申请日：2016-12-16
发明人： Mwita Phelps , Paul Gulde , Richard Fike , Mary Reynolds , Richard Hassett , Andrew Campbell
摘要： The present invention is directed generally to dry cell culture media or feeds in pellet formats which can be reconstituted into liquid media for culturing cells in vitro. Each pellet composition may comprise the same or a different composition; for example, different vitamins, amino acids, buffers, trace salts, pH, iron chelators, etc. The invention also relates to methods of making dry cell culture media by altering ratios of different pellet compositions, or, methods of making modular dry cell culture media, or customizing media formulations for growing a cell type using pellets. According to the invention, media pellets may be easier to handle either before reconstitution, during shipping and handling; and/or during reconstitution. Media pellets may be used in any container like bags including sterile, single use bags for preparing media formulations. The invention also relates to kits and culture systems using media pellets.
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公开(公告)号：US20190048312A1
公开(公告)日：2019-02-14
申请号：US16035344
申请日：2018-07-13
摘要： The present invention relates to nutritive medium, medium supplement, media subgroup and buffer formulations. The present invention provides powder nutritive medium, medium supplement and medium subgroup formulations, e.g., cell culture medium supplements (including powdered sera such as powdered fetal bovine serum (FBS)), medium subgroup formulations and cell culture media comprising all of the necessary nutritive factors that facilitate the in vitro cultivation of cells. The invention further provides powder buffer formulations that produce particular ionic and pH conditions upon reconstitution with a solvent. The invention provides methods for production of media, media supplement, media subgroup and buffer formulations, and also provides kits and methods for cultivation of prokaryotic and eukaryotic cells, particularly bacterial cells, yeast cells, plant cells and animal cells (including human cells) using these dry powder nutritive media, media supplement, media subgroup and buffer formulations.
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公开(公告)号：US20150064785A1
公开(公告)日：2015-03-05
申请号：US14367413
申请日：2012-12-21
发明人： Richard Fike , Shawn Barrett , Zhou Jiang , David Judd
CPC分类号： C12N5/005 , B01J13/06 , B01J13/22 , B29C67/24 , B29L2009/00 , C12N5/0018 , C12N2500/30 , C12N2500/38 , C12N2511/00 , C12N2533/40 , C12N2533/74
摘要： Compositions and methods are described for preparing media, feeds, and supplements. Such methods and medias may display increased stability of labile components and may use, for example, microsuspension and/or encapsulation technologies, chelation, and optionally, coating and/or mixing the labile compounds with anti-oxidants. The compositions may withstand thermal and/or irradiation treatment and have reduced virus number. These techniques may result in product with extended shelf-life, extended release of their internal components into culture, or in product that can be added aseptically into a bioreactor using minimal volumes. The compositions and methods may optimize the bioproduction workflow and increase efficiency.
摘要翻译： 描述了制备培养基，饲料和补品的组合物和方法。 这样的方法和介质可以显示不稳定组分的增加的稳定性，并且可以使用例如微悬浮和/或封装技术，螯合，以及任选地，将不稳定化合物与抗氧化剂包衣和/或混合。 组合物可以承受热和/或照射治疗并且具有减少的病毒数量。 这些技术可能导致产品具有延长的保质期，将其内部组分延长释放到培养物中，或可以无菌地添加到生物反应器中使用最小体积的产品中。 组合物和方法可以优化生物生产工作流程并提高效率。
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公开(公告)号：US20130295667A1
公开(公告)日：2013-11-07
申请号：US13847961
申请日：2013-03-20
发明人： Stephen GORFIEN , Richard Fike , Glenn Godwin , Joyce Dzimian , David Epstein , Dale Gruber , Paul Price
CPC分类号： C12N5/0682 , C07K14/61 , C12N5/0037 , C12N5/0043 , C12N5/005 , C12N5/0603 , C12N7/00 , C12N9/2402 , C12N9/2471 , C12N15/85 , C12N2500/14 , C12N2500/16 , C12N2500/22 , C12N2500/24 , C12N2500/32 , C12N2500/34 , C12N2500/35 , C12N2500/38 , C12N2500/74 , C12N2500/76 , C12N2500/90 , C12N2500/95 , C12N2501/90 , C12N2501/91 , C12N2710/10051 , C12Y302/01023
摘要： The present invention provides a cell culture medium formulation that supports the in vitro cultivation, particularly in suspension, of mammalian cells, particularly epithelial cells and fibroblast cells, and methods for cultivating mammalian cells in suspension in vitro using these media. The media comprise a basal medium and a polyanionic or polyanionic compound, preferably a polysulfonated or polysulfated compound, and more preferably dextran sulfate. The present invention also provides chemically defined, protein-free eukaryotic cell culture media comprising an iron chelate and zinc, which is capable of supporting the growth (and particularly the high-density growth of mammalian cells) in suspension culture, increasing the level of expression of recombinant protein in cultured cells, and/or increasing virus production in cultured cells.
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